Advanced SearchSearch Tips
In vitro Culture Conditions for the Mouse Preantral Follicles Isolated by Enzyme Treatment
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
In vitro Culture Conditions for the Mouse Preantral Follicles Isolated by Enzyme Treatment
Kim, Dong-Hoon; Seong, Hwan-Hoo; Lee, Ho-Joon;
  PDF(new window)
In order to investigate the factors affecting the culture of mouse preantral follicles in vitro, we examined the effect of culture media, protein supplements, and culture period on their growth. The oocyte diameter (initial size: ) was progressively increased during culture, and the maximum size () was reached on day 10 of the in vitro culture. The chromatin configuration in the germinal vesicle (GV) oocyte progressively shifted from a non-surrounded nucleolus (NSN) to a surrounded nucleolus (SN). On day 10 of the culture, most of the oocytes progressed to the SN pattern. The survival and metaphase II rates of the oocytes in alpha-minimal essential medium (alpha-MEM) were significantly higher (p<0.05) than those in Waymouth and tissue culture medium (TCM)-199. As a protein source, fetal bovine serum (FBS) was more suitable for the culture of mouse preantral follicles as compared to human follicular fluid (hFF) and bovine serum albumin (BSA); the optimal concentration of FBS was 5%. These results suggest that in a culture of mouse preantral follicles, alpha-MEM and 5% FBS are an optimal medium and a protein source, respectively; further, the 10 days of culture is required for the complete growth of oocytes in this culture system.
Chromatin Pattern;Media;Mouse Preantral Follicle;Protein;
 Cited by
In Vitro Growth of Bovine Preantral Follicle under Different Culture Conditions,;;;;;;;;;

Reproductive and Developmental Biology, 2009. vol.33. 4, pp.189-194
Expression Levels of mRNA-Encoding PDGF Receptors in Goat Ovaries and the Influence of PDGF on the In Vitro Development of Caprine Pre-Antral Follicles, Reproduction in Domestic Animals, 2011, 47, 5, 695  crossref(new windwow)
Boland, N. I., P. G. Humpherson, H. J. Leese and R. G. Gosden. 1993. Pattern of lactate production and steroidogenesis during growth and maturation of mouse ovarian follicles in vitro. 48:798-806. crossref(new window)

Bouniol-Baly, C., L. Hamraoui, J. Guibert, N. Beaujean, M. S. Szollosi and P. Debey. 1999. Differential transcriptional activity associated with chromatin configuration in fully grown mouse germinal vesicle oocytes. Biol. Reprod. 60:580-587. crossref(new window)

Carroll, J., D. G. Whittingham and M. J. Wood. 1991. Effect of dibutyryl cyclic adenosin monophosphate on granulosa cell proliferation, oocyte growth and meiotic maturation in isolated mouse primary ovarian follicles cultured in collagen gels. J. Reprod. Fertil. 92:197-207. crossref(new window)

Choi, J. K., J. H. Lee, S. T. Lee, M. H. Choi, S. P. Gong, E. J. Lee and J. M. Lim. 2007. Developmental competence of intrafollicular oocytes derived from preantral follicle culture with different protocols after parthenogenetic activation. Asian-Aust. J. Anim. Sci. 20:1190-1195.

Christmann, L., T. Jung and R. M. Moor. 1994. MPF components and meiotic competence in growing pig oocytes. Mol. Reprod. Dev. 38:85-90. crossref(new window)

Cortvrindt, R., J. Smitz and A. C. Van Steirteghem. 1996. In-vitro maturation, fertilization and embryo development of immature oocytes from early preantral follicles from prepuberal mice in a simplified culture system. Hum. Reprod. 11:2656-2666.

Debey, P., M. S. Szollosi, D. Szollosi, D. Vautier, A. Girousse and D. Besombes. 1993. Competent mouse oocytes isolated from antral follicles exhibit different chromatin organization and follow different maturation dynamics. Mol. Reprod. Dev. 36:59-74. crossref(new window)

Eppig, J. J. and S. M. Downs. 1987. The effect of hypoxanthine on mouse oocyte growth and development in vitro: maintenance of meiotic arrest and gonadotrophin-induced oocyte maturation. Dev. Biol. 119:313-321. crossref(new window)

Eppig, J. J. and A. C. Schroeder. 1989. Capacity of mouse oocytes from preantral follicles to undergo embryogenesis and development to live young after growth, maturation and fertilization in vitro. Biol. Reprod. 41:268-276. crossref(new window)

Eppig, J. J., A. C. Schroeder and M. J. O'Brien. 1992. Developmental capacity of mouse oocytes matured in vitro: effects of gonadotrophic stimulation, follicular origin and oocyte size. J. Reprod. Fert. 95:119-127. crossref(new window)

Gutierrez, C. G., J. H. Ralph, E. E. Telfer, I. Wilmut and R. Webb. 2000. Growth and antrum formation of bovine preantral follicles in long-term culture in vitro. Biol. Repord. 62:1322- 1328. crossref(new window)

Harada, M., T. Miyano, K. Matsumura, S. Osaki, M. Miyake and S. Kato. 1997. Bovine oocytes from early antral follicles grow to meiotic competence in vitro: effect of FSH and hypoxanthine. Theriogenol. 46:743-755.

Hartshorne, G. M. 1997. In vitro culture of ovarian follicles. Rev. Reprod. 2:94-104. crossref(new window)

Hartshorne, G. M., I. L. Sargent and D. H. Barlow. 1994. Meiotic progression of mouse oocytes throughout follicle growth and ovulation in vitro. Hum. Reprod. 9:352-359.

Hirao, Y., T. Miyano and S. Kato. 1990. Fertilization of in vitro grown mouse oocytes. Theriogenol. 34:1071-1077. crossref(new window)

Hulshof, S. C., J. R. Figueiredo, J. F. Beckers, M. M. Bevers, J. A. van der Donk and R. van den Hurk. 1995. Effects of fetal bovine serum, FSH and 17beta-estradiol on the culture of bovine preantral follicles. Theriogenol. 44:217-226. crossref(new window)

Johnson, L. D., D. F. Albertini, L. K. McGinnis and J. D. Biggers. 1995. Chromatin organization, meiotic status and meiotic competence acquisition in mouse oocytes from cultured ovarian follicles. J. Reprod. Fertil. 104:277-284. crossref(new window)

Kim, D-H., D-S. Ko, H-C. Lee, H-J. Lee, W-I. Park, S. S. Kim, JK. Park, B-C. Yang, S-B. Park, W-K .Chang and H-T. Lee. 2004. Comparison of maturation, fertilization, development, and gene expression of mouse oocytes grown in vitro and in vivo. J. Assist. Reprod. Genet. 21:233-240. crossref(new window)

Li, R., D. M. Phillips and J. P. Mather. 1995. Activin promotes ovarian follicle development in vitro. Endocrinol. 136:849-856. crossref(new window)

Liu, X., K. Andoh, H. Yokota, J. Kobayashi, Y. Abe, K. Yamada, H. Mizunuma and Y. Ibuki. 1998. Effects of growth hormone, activin and follistatin on the development of preantral follicle from immature female mice. Endocrinol. 139:2342-2347. crossref(new window)

Mattson, B. A. and D. F. Albertini. 1990. Oogenesis: chromatin and microtubule dynamics during meiotic prophase. Mol. Reprod. Dev. 25:374-383. crossref(new window)

Nayudu, P. L. and S. M. Osborn. 1992. Factors influencing the rate of preantral and antral growth of mouse ovarian follicles in vitro. J. Reprod. Fertil. 95:349-362. crossref(new window)

Qvist, R., L. F. Blackwell, H. Bourne and J. B. Brown. 1990. Development of mouse ovarian follicles from primary to preovulatory stages in vitro. J. Reprod. Fertil. 89:169-180. crossref(new window)

Roy, S. K. and B. J. Treacy. 1993. Isolation and long-term culture of human preantral follicles. Fertil. Steril. 59:783-790.

Spears, N., N. I. Boland, A. A. Murray and R. G. Gosden. 1994. Mouse oocytes derived from in vitro grown primary ovarian follicles are fertile. Hum. Reprod. 9:527-532.

Torrance, C., E. Telfer and R. G. Gosden. 1989. Quantitative study of the development of isolated mouse pre-antral follicles in collagen gel culture. J. Reprod. Fertil. 87:367-374. crossref(new window)

Wickramasinghe, D., K. M. Ebert and D. F. Albertini. 1991. Meiotic competence acquisition is associated with the appearance of M-phase characteristics in growing mouse oocytes. Dev. Biol. 143:162-172. crossref(new window)

Zuccotti, M., P. Giorgi Rossi, A. Martinez, S. Garagna, A. Forabosco and C. A. Redi. 1998. Meiotic and developmental competence of mouse antral oocytes. Biol. Reprod. 58:700-704. crossref(new window)

Zuccotti, M., A. Piccinelli, P. Giorgi Rossi, S. Garagna and C. A. Redi. 1995. Chromatin organization during mouse oocyte growth. Mol. Reprod. Dev. 41:479-485. crossref(new window)