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The Effect of Roselle (Hibicus sabdariffa Linn.) Calyx as Antioxidant and Acidifier on Growth Performance in Postweaning Pigs
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 Title & Authors
The Effect of Roselle (Hibicus sabdariffa Linn.) Calyx as Antioxidant and Acidifier on Growth Performance in Postweaning Pigs
Aphirakchatsakun, Wantana; Angkanaporn, Kris; Kijparkorn, Suwanna;
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Two experiments, involving a total of 100 crossbred pigs (HampshireLandraceDuroc) aged 5 weeks, were used to evaluate the effect of Roselle (Hibicus sabdariffa Linn.) calyx as an antioxidant and acidifier on growth performance. Experiment 1: growth performance response of pigs fed basal corn-soy diet was compared with that of pigs consuming diets that contained 4, 8 and 12% Roselle in powder form, 4 g/kg acidifier (FraAcid Dry) or 100 mg/kg antibiotic (Aurofac). All diets were isocaloric and isonitrogenous. Twenty-four castrated male and twelve female pigs were randomly allocated into 6 treatments which comprised 3 replicates (2 male and 1 female) of 2 pigs each. The results demonstrated no beneficial weight gain and feed intake response among treatments at 7, 9 and 11 weeks of age (p>0.05). However, feed-to-gain ratios (FCR) of Roselle-fed groups were significantly different from the basal-diet group at 7 weeks of age (p<0.05). Pigs fed with 8% Roselle had the lowest FCR. Therefore, 8% was set as an appropriate level of Roselle in feed. Experiment 2 was conducted to determine antioxidant and acidifier properties. Pigs were fed similar diets to Experiment 1 except that Roselle was fed to only one treatment at the level of 8%. Sixty-four piglets, 32 castrated male and female each were randomly allocated into 4 treatments which comprised 4 replicates (2 male and 2 female) of 4 pigs each. Body weight was measured. At 7 and 9 weeks of age, four pigs from each treatment were randomly selected. The pH in their gastrointestinal tract, pepsin activity in stomach mucosa, trypsin activity in the pancreas and protein and fat digestibility in the ileum were measured to investigate acidifier properties. For antioxidant properties, malondialdehyde (MDA) and glutathione concentration in plasma and liver were used as parameters. The results showed no significant difference in weight gain, pH or pepsin activity among all treatments at both ages (p>0.05). Nevertheless, trypsin activity and fat digestibility of the Roselle-fed group were higher than the basal-diet group at 7 weeks of age (p<0.05). Glutathione in plasma was significantly different between antibiotic-fed and basal-diet groups (p<0.05). Significant differences were found only in some parameters and between the basal-diet group and the other groups. Therefore, it cannot be precisely concluded that Roselle in powder form has clear acidifier and antioxidant properties in postweaning pigs. Further studies should be undertaken to clearly confirm both properties.
Roselle;Antioxidant;Acidifier;Growth Performances;Enzyme Activity;Malondialdehyde;Ileal Digestibility;Postweaning Pigs;
 Cited by
Ali, B. H., H. M. Mousa and E. S. Mougy. 2003. The effect of water extract and anthocayanins of Hibicus sabdariffa L. on paracetamol induced hepatoxicity in rats. Phyto. Res. 17 (Suppl.1):56-59 (Abstr.). crossref(new window)

AOAC. 1990. Methods of Analysis. 15th edn. Association of Official Analytical Chemists, Arington, Virginia.

Beutler, E., O. Duron and B. Mikus. 1963. Improved method for the determination of blood glutathione. J. Lab. Clin. Med. 16:882-889.

Cera, K. R., D. C. Mahan and G. A. Reinhart. 1989. Apparent fat digestibility and performance responses of postweaning swine fed diets supplemented with coconut oil, corn oil or tallow. J. Anim. Sci. 67:2040.

Choct, M. and G. Annison. 1992. The inhabitation of nutrient digestion by wheat pentosans. Br. J. Nutr. 67:123-132. crossref(new window)

Chu, M. N., R. C. Solevilla, B. Q. Guevera and P. S. Santos. 1987. Antimicrobial properties of Hibicus sabdariffa. Acta Manila. 36:3-5.

Cranwell, P. D. 1995. Development of the neonatal gut and enzyme systems. pp. 99-154 in The Neonatal Pig. Development and Survival. Wallingford, Oxon. CAB International.

Dibner, J. J. and P. Buttin. 2002. Use of organic acids as a model to study the impact of gut microflora on nutrition and metabolism. J. Appl. Poult. Res. 11:453-463.

Doyle, M. E. 2001. Alternatives to antibiotic use for growth promotion in animal husbandry. Food research institute, university of Wisconsin-Madison. pp. 1-17. http://www.wise. edu/fri/briefs/antibiot.pdf.

Duh, P. D. and G. C. Yen. 1997. Antioxidative activity of three herbal water extracts. Food Chem. 60(4):639-645. crossref(new window)

Falkowski, J. F. and F. X. Aherne. 1984. Fumaric and citric acid as feed additives in starter pig nutrition. J. Anim. Sci. 58:935.

Gauthier, R. 2002. Intestinal health, the key to productivity (the case of organic acids). Precongreso Cientifico Avicola IASA. Mexico.

Gupta, Y. K., M. H. Veerendra and A. K. Srivastava. 2003. Effect of Centella asiatica on pentylenetetrazole-induced kindling, cognition and oxidative stress in rats. Pharmacol. Biochem. Behav. 74:579-585. crossref(new window)

Kemme. 1998. The relationship between graded doses of Lupro- Cid and the apparent total tract, digestibility of total phosphorus and calcium in growing pigs. http://www. 01600011.pdf.

Kil, D.Y., L.G. Piao, H. F. Long, J. S. Lim, M. S. Yun, C. S. Kong, W. S. Ju, H. B. Lee and Y. Y. Kim. 2006. Effects of organic or inorganic acid supplementation on growth performance, nutrient digestibility and white blood cell counts in weanling pigs. Asian-Aust. J. Anim. Sci. 19:252-261.

Kirchgessner, M. and F. X. Roth. 1982. Fumaric acid as a feed additive in pig nutrition. Pig News Info. 3:259.

Kommera, S. K., R. D. Mateo, F. J. Neher and S. W. Kim. 2006. Phytobiotics and organic acids as potential alternatives to the use of antibiotics in nursery pigs diets. Asian-Aust. J. Anim. Sci. 19:1784-1789.

Kramonwan, S. and S. Narumon. 2002. Study on Antibacterial activity against Diarrheal Bacterias of Thai Medicinal Plants. Project of Pharmacology. Chulalongkorn University. pp. 26 (In Thais).

Liu, C. L., J. M. Wang, C. Y. Chu, M. T. Cheng and T. H. Tseng. 2002. In vivo protective effect of protocatechuic acid on tertbutyl hydroperoxide-induced rat hepatotoxicity. Food. Chem. Toxicol. 40:635-641. crossref(new window)

Lowry, O. H., N. J. Rosebrough, A. L. Farr and R. Randall. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193:265-275.

Mahady, G. B. 2002. Are medicinal plants a potential alternative for conventional antibiotics in animal husbandry? Review Article. Thai. J. Phyto. 9(1):30-62.

May, R. W. and J. M. Bell. 1971. Digestible and metabolizable energy values of some feeds for the growing pigs. Can. J. Anim. Sci. 51:271-278. crossref(new window)

Mayerly, N., F. Romero, Y. Quiroz, G. Parra, L. Bonet and B. Rodriguez-iturbe. 2000. Melatonin attenuates actue renal failure and oxidative stress induced by mercuric chloride in rats. Anim. Physiol. Renal. Phystol. pp. 910-918.

Nanthawan, B. and C. Auranuch. 1998. Thai native Herbs No 1. Bangkok, Prachachon Press. pp. 14-18 (In Thais).

National Research Council, NRC. 1998. Nutrient Requirements of Swine. 10th Ed. National Academy Press, Washington, DC.

Noblet, J. and J. M. Perez. 1993. Prediction of digestibility of nutrients and energy values of pig diets from chemical analysis. J. Anim. Sci. 71:3389-3398.

Ohkawa, H., N. Ohishi and K. Yagi. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95:351-358. crossref(new window)

Partanen, H. K. and Z. Mroz. 1999. Organic acids for performance enhancement in pig diets. Nutr. Rev. 12:117-145. crossref(new window)

Peris, S. and F. Calafat. 1994. Acidification and other physiological additives. Cahe. Opt. Medit. 54:63-70.

Pettigrew, J. E., Jr. and R. L. Moser. 1991. Fat in swine nutrition. pp. 133-146 in Swine Nutrition (Ed. E. R. Miller, D. E. Ullrey and A. J. Lewis). Stoneham, UK: Butterworth-Heinemann.

Ravindran, V. and E. T. Kornegay. 1993. Acidification of weaned pig diets: A review. J. Sci. Food Agric. 62:313-940. crossref(new window)

Rick, W. 1965. Methods of enzymatic analysis. Academic press. NewYork and London. pp. 807-814.

Sales, J. and G. P. J. Janssens. 2003. Acid insoluble ash as a marker in digestibility studies. J. Anim. Feed Sci. 12(3):383-401.

SAS Institue Inc. 1989. SAS/STAT User's Guide: Version 6. 4th edn. SAS Institue Inc., Cary. North Carolina.

Stahly, T. S. 1984. Use of fats in diets for growing pigs. pp. 313- 331 in Fat in Animal Nutrition (Ed. J. Wise), London: Butterworth.

Steel, R. G. D. and J. H. Torrie. 1980. Principles and Procedures of Statistics: A Biometrical Approach. 2nd edn. McGraw-Hill Book Company, New York.

Thaela, M. J., M. S. Jenson, S. G. Pierzynowski, S. Jakob and B. B. Jenson. 1998. Effect of lactic acid supplementation on pancreatic secretion in pigs after weaning. J. Anim. Feed Sci. 7 (Suppl. 1):181-183 (Abstr.).

Tsai, P. J., J. Mcintosh, B. Camden and B. R. Jordan. 2002. Anthocyanin and antioxidant capacity in Roselle (Hibicus sabdariffa Linn) extract. Food Res. Inter. 35:351-356. crossref(new window)

Tseng, T. H., E. S. Kao, C. Y. Chu, F. P. Chou, W. L. Lin and C. J. Wang. 1997. Protective effects of dried flower extracts of Hibicus sadbariffa L. against oxidative stress in rat primary hepatocytes. Food Chem. Toxicol. 35:1159-1164. crossref(new window)

Wang, C. J., J. M. Wang, W. L. Lin, C. Y. Chu, F. P. Chou and T. H. Tseng. 2000. Protective effect of Hibicus anthocyanins against tert-butyl hydroxide-induced hepatic toxicity in rats. Food Chem. Toxicol. 38:411-416. crossref(new window)

Wilson, R. H. and J. Leibholz. 1981. Digestive in the pig between 7 and 35 days of age. 1. The performance of pigs given milk and soybean proteins. Br. J. Nutr. 45:301-319. crossref(new window)