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Improvement of Functional Properties of Ovotransferrin by Phosphorylation through Dry-heating in the Presence of Pyrophosphate
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 Title & Authors
Improvement of Functional Properties of Ovotransferrin by Phosphorylation through Dry-heating in the Presence of Pyrophosphate
Hayashi, Yoko; Li, Can-Peng; Enomoto, Hirofumi; Ibrahim, Hisham R.; Sugimoto, Yasushi; Aoki, Takayoshi;
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 Abstract
Ovotransferrin (OTf) was phosphorylated by dry-heating in the presence of pyrophosphate at pH 4.0 and for 1 and 5 d, and the functional properties of phosphorylated OTf (PP-OTf) were investigated. The phosphorus content of OTf increased to 0.91% as a result of phosphorylation and the electrophoretic mobility of PP-OTf also increased. Although the solubility of dry-heated OTf slightly decreased, the decrease was reduced by phosphorylation. The stability against heat-induced insolubilization of OTf was somewhat improved by phosphorylation, but more than 70% of PP-OTf was insolubilized when it was heated at for 10 min at pH 7.0. However, heat-induced insolubilization of PP-OTf was reduced when it was heated in the presence of phosphorylated ovalbumin. This may explain the excellent stability of phosphorylated egg white protein against heat-induced insolubilization which was reported previously. The emulsifying property of OTf was also somewhat improved by phosphorylation. The calcium phosphate-solubilizing ability of PP-OTf was enhanced. Although the degree of phosphorylation of OTf by dry-heating in the presence of pyrophosphate was similar to that of ovalbumin, the improvement of properties of PP-OTf was considerably different from those of phosphorylated ovalbumin.
 Keywords
Ovotransferrin;Phosphorylation;Dry-heating;Functional Property;
 Language
English
 Cited by
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 References
1.
Aoki, T. 1989. Incorporation of individual casein constituents into casein aggregates cross-linked by colloidal calcium phosphate in artificial casein micelles. J. Dairy Res. 56:613-618. crossref(new window)

2.
Aoki, T., K. Kitahata, T. Fukumoto, Y. Sugimoto, H. R. Ibrahim, T. Kimura, Y. Kato and T. Matuda. 1997. Improvement of functional properties of $\beta$-lactoglobulin by conjugation with glucose-6-phosphate through the Maillard reaction. Food Res. Int. 30:401-406. crossref(new window)

3.
Aoki, T., T. Fukumoto, T. Kimura, Y. Kato and T. Matsuda. 1994. Whey protein- and egg white protein-glucose 6-phosphate conjugates with calcium phosphate-solubilizing properties. Biosci. Biotecnol. Biochem. 58:1727-1728. crossref(new window)

4.
Campbell, N. F., F. F. Shih and W. E. Marshall. 1992. Enzymatic phosphorylation of soy protein isolate for improved functional properties. J. Agric. Food Chem. 40:403-406. crossref(new window)

5.
Chen, P. S., T. Y. Toribara and H. Warner. 1956. Microdetermination of phosphorus. Anal. Chem. 28:1756-1758. crossref(new window)

6.
Feeney, R. E. 1975. Chemical changes in food proteins. In: Evaluation of proteins for humans (Ed. C. E. Bodwell). AVI Publishers, Westport, CT. pp. 233-254.

7.
Kato, A., H. R. Ibrahim, H. Watanabe, K. Honma and K. Kobayashi. 1989. New approach to improve the gelling and surface functional properties of dried egg white by heating in dry state. J. Agric. Food Chem. 37:433-437. crossref(new window)

8.
Kato, Y., T. Aoki, N. Kato, R. Nakamura and T. Matsuda. 1995. Modification of ovalbumin with glucose 6-phosphate by amino-carbonyl reaction. Improvement of protein heat stability and emulsifying activity. J. Agric. Food Chem. 43:301-305. crossref(new window)

9.
Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:680-685. crossref(new window)

10.
Li, C. P., A. S. Salvador, H. R. Ibrahim, Y. Sugimoto and T. Aoki. 2003. Phosphorylation of egg white proteins by dry-heating in the presence of phosphate. J. Agric. Food Chem. 51:6808-6815. crossref(new window)

11.
Li, C. P., H. R. Ibrahim, Y. Sugimoto, H. Hatta and T. Aoki. 2004. Improvement of functional properties of egg white protein through phosphorylation by dry-heating in the presence of pyrophosphate. J. Agric. Food Chem. 52:5752-5758. crossref(new window)

12.
Li, C. P., Y. Hayashi, H. Shinohara, H. R. Ibrahim, Y. Sugimoto, J. Kurawaki, N. Matsudomi and T. Aoki. 2005. Phosphorylation of ovalbumin by dry-heating in the presence of pyrophosphate: Effect on protein structure and some properties. J. Agric. Food Chem. 53:4962-4967. crossref(new window)

13.
Lowry, O. H., N. J. Rosebrough, A. L. Farr and R. J. Randall. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193:265-275.

14.
Matheis, G. 1991. Phosphorylation of food proteins with phosphorus oxychloride-Improvement of functional and nutritional properties: A review. Food Chem. 39:13-26. crossref(new window)

15.
Matheis, G. and J. R. Whitaker. 1984. Chemical phosphorylation of food proteins: An overview and a prospectus. J. Agric. Food Chem. 32:699-705. crossref(new window)

16.
Matsuda, T., K. Watanabe and Y. Sato. 1981. Heat-induced aggregation of egg white proteins as studied by vertical flatsheet polyacrylamide gel electrophoresis. J. Food Sci. 46:1829-1834. crossref(new window)

17.
Pearce, K. N. and J. E. Kinsella. 1978. Emulsifying properties of proteins: Evaluation of a turbidimetric technique. J. Agric. Food Chem. 26:716-723. crossref(new window)

18.
Seguro, K. and M. Motoki. 1989. Enzymatic phosphorylation of soybean proteins by protein kinase. Agric. Biol. Chem. 53:3263-3268. crossref(new window)

19.
Sitohy, M., J.-M. Chobert and T. Haertle. 1995. Phosphorylation of $\beta$-lactoglobulin under mild conditions. J. Agric. Food Chem. 43:59-62. crossref(new window)

20.
Watanabe, K., J. Q. Xu and M. Shimoyamada. 1999. Inhibiting effect of egg white dry-heated at $120^{\circ}C$ on heat aggregation and coagulation of egg white and characteristics of dry-heated egg white. J. Agric. Food Chem. 47:4083-4088. crossref(new window)

21.
Yamashita, H., J. Ishibashi, Y.-H. Hong and M. Hirose. 1998. Involvement of ovotransferrin in the thermally induced gelation of egg white at around $65^{\circ}C$, Biosci. Biotechnol. Biochem. 62:593-595. crossref(new window)