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Isolation and Characterization of Microsatellite Markers in Tsaiya Duck
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 Title & Authors
Isolation and Characterization of Microsatellite Markers in Tsaiya Duck
Hsiao, M.C.; Liu, H.C.; Hsu, Y.C.; Hu, Y.H.; Li, S.H.; Lee, S.R.;
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An enrichment library of GATA-repeats from genomic DNA was constructed in this study to isolate and characterize microsatellite loci in Tsaiya duck (Anas platyrhynchos). Thirty-three microsatellite markers were developed and used to detect polymorphisms in 30 Tsaiya ducks. A total of 177 alleles were observed and all loci except APT022 were polymorphic. The number of alleles ranged from 2 to 9 with an average of 5.5 per microsatellite locus. The observed and expected heterozygosity of these polymorphic markers ranged from 0.07 to 0.93 with an average number of 0.60 and 0.10 to 0.86 with an average number of 0.61, respectively. Among the polymorphic markers, the observed heterozygosities of 23 loci were higher than 0.50 (69.70%). The polymorphism information content (PIC) in the 32 loci ranged from 0.09 to 0.83 with an average of 0.57. Seven of the 33 duck microsatellite loci had orthologs in the chicken genome, but only APT004 had a similar core repeat to chickens. These microsatellite markers will be useful in constructing a genetic linkage map for the duck and a comparative mapping with the chicken can also provide a valuable tool for studies related to biodiversity and population genetics in this duck species.
Microsatellite Marker;Polymorphism;Tsaiya Duck;
 Cited by
Botstein, D., R. L. White, M. Skolnick and R. W. Davis. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am. J. Hum. Genet. 32:314-331.

Denk, A. G., B. Gautschi, K. Carter and B. Kempenaers. 2004. Seven polymorphic microsatellite loci for paternity assessment in the mallard (Anas platyrhynchos). Mol. Ecol. Notes 4:506-508. crossref(new window)

Guay, P. -J. and R. A. Mulder. 2005. Isolation and characterization of microsatellite markers in musk duck (Biziura lobata: Aves), and their application to other waterfowl species. Mol. Ecol. Notes 5:249-252. crossref(new window)

Hamilton, M. B., E. L. Pincus, A. Di Fiore and R. C. Fleischer. 1999. Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites. Biotechniques 27:500-507.

Hsu, Y. C., L. L. Severinghaus, Y. S. Lin and S. H. Li. 2003. Isolation and characterization of microsatellite DNA markers from the Lanyu scops owl (Otus elegans botelensis). Mol. Ecol. Notes 3:595-597. crossref(new window)

Huang, Y. H., J. F. Tu, X. B. Cheng, B. Tang, X. X. Hu, Z. L. Liu, J. D. Feng, Y. K. Lou, L. Lin, K. Xu, Y. L. Zhao and N. Li. 2005. Characterization of 35 novel microsatellite DNA markers from the duck (Anas platyrhynchos) genome and cross-amplification in other birds. Genet. Sel. Evol. 37:455-472. crossref(new window)

Huang, Y. H., Y. H. Zhao, C. S. Haley, S. P. Hu, J. P. Hao, C. X. Wu and N. Li. 2006. A genetic and cytogenetic map for the duck (Anas platyrhynchos). Genetics 173:287-296. crossref(new window)

Kong, H. S., J. D. Oh, J. H. Lee, K. J. Jo, B. D. Sang, C. H. Choi, S. D. Kim, S. J. Lee, S. H. Yeon, G. J. Jeon and H. K. Lee. 2006. Genetic variation and relationships of korean native chickens and foreign breeds using 15 microsatellite markers. Asian-Aust. J. Anim. Sci. 19:1546-1550.

Liu, G. Q., X. P. Jiang, J. Y. Wang and Z. Y. Wang. 2006. Correlations between heterozygosity at microsatellite loci, mean d2 and body weight in a chinese native chicken. Asian-Aust. J. Anim. Sci. 19:1671-1677.

Maak, S., K. Neumann, G. von Lengerken and R. Gattermann. 2000. First seven microsatellites developed for the Peking duck (Anas platyrhynchos). Anim. Genet. 31:228-241. crossref(new window)

Maak, S., K. Wimmers, S. Weigend and K. Neumann. 2003. Isolation and characterization of 18 microsatellites in the Peking duck (Anas platyrhynchos) and their application in other waterfowl species. Mol. Ecol. Notes 3:224-227. crossref(new window)

Marshall, T. C., J. Slate, L. E. B. Kruuk and J. M. Pemberton. 1998. Statistical confidence for likelihood-based paternity inference in natural populations. Mol. Ecol. 7:639-655. crossref(new window)

Meuwissen, T. H. E. and M. E. Goddard. 1996. The use of marker haplotypes in animal breeding schemes. Genet. Sel. Evol. 28:161-176. crossref(new window)

Munoz-Fuentes, V., N. Gyllenstrand, J. J. Negro, A. J. Green and C. Vila. 2005. Microsatellite markers for two stifftail ducks: the white-headed duck, Oxyura leucocephala, and the ruddy duck, O. jamaicensis. Mol. Ecol. Notes 5:263-265. crossref(new window)

Paulus, K. B. and R. Tiedemann. 2003. Ten polymorphic autosomal microsatellite loci for Eider duck Somateria mollissima and their cross-species applicability among waterfowl species (Anatidae). Mol. Ecol. Notes 3:250-252. crossref(new window)

Reed, K. M., J. Holm, M. Morrisson, S. Leroux and A. Vignal. 2005. Assignment of non-informative turkey genetic markers through comparative approaches. Cytogenet. Genome Res. 109:527-532. crossref(new window)

Stai, S. M. and C. R. Hughes. 2003. Characterization of microsatellite loci in wild and domestic Muscovy ducks (Cairina moschata). Anim. Genet. 34:387-389.

Stallings, R. L., A. F. Ford, D. Nelson, D. C. Torney, C. E. Hildebrand and R. K. Moyzis. 1991. Evolution and distribution of $(GT)_n$ repetitive sequences in mammalian genomes. Genomics 10:807-815. crossref(new window)