Advanced SearchSearch Tips
Effect of Chromium Dietary Supplementation on the Immune Response and Some Blood Biochemical Parameters of Transport-stressed Lambs
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effect of Chromium Dietary Supplementation on the Immune Response and Some Blood Biochemical Parameters of Transport-stressed Lambs
Al-Mufarrej, S.I.; Al-Haidary, I.A.; Al-Kraidees, M.S.; Hussein, M.F.; Metwally, H.M.;
  PDF(new window)
Forty-eight Naemi lambs (avg. BW 31.7 kg) were transported by truck for a distance of 1,450 km from Al-Jouf to Riyadh, Saudi Arabia. On arrival day, the lambs were randomly allocated to four groups receiving diets supplemented with 0.0, 0.3, 0.6 and 0.9 ppm organic chromium (Cr). Each group consisted of four separately housed replicates of three lambs each. The animals were fed ad libitum on a grower diet for 84 days. Blood samples were obtained shortly before transportation, upon arrival and at weekly intervals thereafter from all lambs for analysis of plasma and serum. Plasma glucose and serum cortisol, total protein, albumin, urea-N and total cholesterol concentrations were determined. A cursory clinical examination of the lambs, along with rectal temperature, was undertaken at different intervals during the experiment. The lambs were inoculated each with 2 ml i.v. chicken red blood cells (CRBC) on days 0, 21, and 42. Serum total, IgG and IgM antibody titers were determined at weekly intervals post-immunization. An in vivo intradermal hypersensitivity test was carried out on 6 lambs from each group on days 10 and 70. Transportation of the lambs resulted in a significant (p<0.001) elevation of serum cortisol, total protein and albumin levels, as well as increased plasma glucose concentration, with corresponding decrease in total cholesterol, while blood urea-N remained largely unchanged. These constituents returned to normal levels during subsequent weeks, with no significant differences in their concentrations being observed between the Cr-supplemented groups and controls. Rise in rectal temperature after transportation was reduced to a greater extent (p<0.05) in Cr-supplemented versus control lambs. Total, IgG and IgM antibody titers against CRBC rose significantly (p<0.05) during immunizations in all groups, with significantly and linearly higher (p<0.05) total and IgG titers in Cr-supplemented versus control lambs. By contrast, no significant effect due to Cr supplementation was recorded in IgG titers, which increased equally in Cr-fed and control groups. Skin thickness in response to intradermal inoculation of phytohaemagglutinin (PHA) was also significantly (p<0.01) increased as a result of Cr supplementation. These results indicate that dietary Cr supplementation might be useful during stress especially for enhancing immune responses in transport-stressed lambs.
High Chromium Yeast;Immune Response;Some Blood Parameters;Lambs;
 Cited by
Effect of Supplemental Chromium Levels on Performance, Digestibility and Carcass Characteristics of Transport-stressed Lambs,;;;;;;

아세아태평양축산학회지, 2009. vol.22. 8, pp.1124-1132 crossref(new window)
Anderson, R. A. 1987. Chromium in Trace Elements in Human and Animal Nutrition. Vol. 15th Ed., (Ed. W. Mertz). pp. 225-244. New York: Academic Press, Inc.

Anderson, R. A. 1994. Stress effects on chromium nutrition of humans and farm animals. Biotechnology in the Feed Industry. Proceedings of Alltech's Tenth Annual Symposium (Ed. T. P. Lyons and K. A. Jacques). pp. 267-274. Nottingham University Press, Nottingham, UK.

Anderson, R. A. 1998. Recent advances in the clinical and biochemical manifestation of chromium deficiency in human and animal nutrition. J. Trace Elem. Exp. Med. 11:241-250. crossref(new window)

Bennett, B. W., R. P. Kerschen and C. F. Nockels. 1989. Stress induced hematological changes in feedlot cattle. Agri-Practice. 10:16-28.

Borgs, P. and B. A. Mallard. 1998. Immune endocrine interactions in agricultural species: chromium and its effects on health and performance. Domestic Anim. Endocrinol. 15(4):431-438. crossref(new window)

Burton, J. L., B. A. Mallard and D. N. Mowat. 1993. Effect of supplemental chromium on immune response of pre-parturient and early lactation dairy cows. J. Anim. Sci. 71:1532-1539.

Chang, X. and D. N. Mowat. 1992. Supplemental chromium for stressed and growing feeder calves. J. Anim. Sci. 70:559-565.

Chang, X., B. A. Mallard and D. N. Mowat. 1994. Proliferation of peripheral blood lymphocytes of feeder calves in response to chromium. Nutr. Res. 14:851-864. crossref(new window)

Chang, X., D. N. Mowat and B. A. Mallard. 1995. Supplemental chromium and niacin for stressed feeder calves. Can. J. Anim. Sci. 75:351-358. crossref(new window)

Cole, N. A., T. H. Camp, L. D. Rowe Jr., D. G. Stevens and D. P. Hutcheson. 1988. Effect of transport on feeder calves. Am. J. Vet. Res. 49:178-183.

Cole, N. A., J. B. McLaren and D. P. Hutcheson. 1982. Influence of preweaning and B-vitamin supplementation of the feedlot receiving diet on calves subjected to marketing and transit stress. J. Anim. Sci. 54:911-917.

Cole, N. A., W. A. Phillips and D. P. Hutcheson. 1986. The effect of pre-fast diet and transport on nitrogen metabolism of calves. J. Anim. Sci. 62:1719-1731.

Fordham, D. P., G. A. Lincoln, A. Ssewannyana and R. G. Rodway. 1989. Plasma $\beta$-Endorphin and cortisol concentrations in lambs after handling, transport and slaughter. Anim. Prod. 49:103-107. crossref(new window)

Galyean, M. L., R. W. Lee and M. E. Hubbert. 1981. Influence of fasting and transit on ruminal blood metabolites in beef steers. J. Anim. Sci. 53:7-18.

Gentry, L. R., J. M. Fernandez, T. L. Ward, T. W. White, L. L. Southern, T. D. Bidner, D. L. Thompson, D. W. Jr., Horohov, A. M. Chapa and T. Sahlu. 1999. Dietary protein and chromium tripicolinate in suffolk wether lambs: effects on production characteristics, metabolic and hormonal responses, and immune status. J. Anim. Sci. 77:1284-1294.

Hutcheson, D. P., N. A. Cole and J. B. McLaren. 1984. Effects of pretransit diets and post-transit potassium levels for feeder calves. J. Anim. Sci. 58:700-707.

Hutcheson, D. P. 1990. Nutrition critical in getting calves started right. Feedstuffs. 62(11):14.

Hutcheson, D. P. and N. A. Cole. 1986. Management of transitstress syndrome in Cattle: Nutritional and environmental effects. J. Anim. Sci. 62:555-560.

Kegley, E. B., J. W. Spears and T. T. Brown, Jr. 1997a. Effect of shipping and chromium supplementation on performance, immune responses, and disease resistance of steers. J. Anim. Sci. 75:1956-1964.

Kegley, E. B., J. W. Spears and Eisemann. 1997b. Performance and glucose metabolism in calves fed a chromium-nicotinic acid complex or chromium chloride. J. Dairy Sci. 80:1744-1750. crossref(new window)

Kent, J. E. and R. Ewbank. 1986. The effect of road transportation on the blood constituents and behaviour of calves. III. Three months old. Br. Vet. J. 142:326-335.

Lindemann, M. D., C. M. Wood, A. F. Harper, E. T. Kornegay and R. A. Anderson. 1995. Dietary chromium picolinate additions improve gain: feed and carcass characteristics in growingfinishing pigs and increase litter size in reproducing sows. J. Anim. Sci. 73:457-465.

Mertz, W. 1993. Chromium in human nutrition: A review. J. Nutr. 123:626-633.

Moonsie-Shageer, S. and D. N. Mowat. 1993. Effect of level of supplemental chromium on performance, serum constituents, and immune status of stressed feeder calves. J. Anim. Sci. 71:232-238.

Mowat, D. N., X. Chang and W. Z. Yang. 1993. Chelated chromium for stressed feeder calves. Can. J. Anim. Sci. 73:49-55. crossref(new window)

Nielson, F. 1994. Chromium. In Modern Nutrition in Health and Disease, 8th ed. Lea & Febiger, Phil. 264-268.

Nockels, C. F. 1994. Understanding stress in cattle. Biotechnolongy in the feed industry: Proceeding of Alltech's 10th. pp. 255-265. Annual Symposium. Leicester shiro UK.

Nockels, C. F. 1990. Effect of stress on mineral requirements. Western Nutr. Conf. p. 27. Calgary, AB, Canada.

NRC. 1985. Nutrient Requirements of sheep (6th Ed.). National Academy Press, Washington, DC.

Okada, S., H. Tsukade and M. Tezuka. 1989. Effect of chromium (III) on nuclear RNA synthesis. Biol. Trace. Elem. Res. 21:35-9. crossref(new window)

SAS Institute. 1998. SAS User's Guide. Version 6.12. SAS Institute, Inc., Cary, NC.

Selye, H. 1956. The stress of life. McGraw Hill Book Co., New York.

Solomon and Delhanty 1966. The nature of antibodies to goat erythrocytes in the developing chicken. Immunology. 1966 Aug; 11(2):103-113.

Thiel, R. J. 1996. Serious Nutrition for Health Care Professionals, 2nd ed. Center for Natural Health Research, Arroyo Grande (CA).

Van Heugten, E. and J. W. Spears. 1997. Immune response and growth of stressed weanling pigs fed diets supplemented with organic or inorganic forms of chromium. J. Anim. Sci. 75:409-416.

Vinson, J. A. and P. Bose. 1984. The effect of high chromium yeast on the blood glucose control and blood lipids of normal and diabetic human subjects. Nutr. Reports Intl, 30(4):911-918.

Ward, T. L., L. L. Southern and T. D. Bidner. 1997. Interactive effects of dietary chromium tripicolinate and crude protein level in growing-finishing pigs provided inadequate and adquate pen space. J. Anim. Sci. 75:1001-1008.

Weser, U. and J. Koolman. 1969. Reactivity of some transition metals on nuclear protein biosynthesis in rat liver. Experientia 26:246-247.

Witlin, B. 1967. Detection of antibodies by microtitration techniques. Mycopath. Mycol. App. 33:241-257. crossref(new window)

Wright, A. J., D. N. Mowat and B. A. Mallard. 1994. Supplemental chromium and bovine respiratory disease vaccines for stressed feeder calves. Can. J. Anim. Sci. 74:287-295. crossref(new window)