Advanced SearchSearch Tips
Regulation of Lipoprotein Lipase by Fasting in Epididymal and Mesenteric Adipocytes of Rats
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Regulation of Lipoprotein Lipase by Fasting in Epididymal and Mesenteric Adipocytes of Rats
Lee, Jae Joon; Chung, Chung Soo; Lee, Myung Yul;
  PDF(new window)
There are marked variations in the activity of lipoprotein lipase (LPL) among adipose depots. The aim of this study was to compare the mechanisms of 24 h of fasting on LPL regulation between epididymal (EPI) adipocytes and mesenteric (MES) adipocytes in rats. 1-Day fasting consistently decreased activities of heparin-releasable LPL, total extractable LPL and cellular LPL markedly in both EPI and MES fat pads. LPL activity in MES fat pads was relatively lower than in the EPI fat pads. Consistent with data on LPL activity, the levels of expression of LPL mRNA in both nutritional states were lower in MES than EPI adipose tissue and isolated adipocytes. The decreased LPL activity after 1 day of fasting in MES adipocytes was explained mainly by a 50% decrease in the relative abundance of LPL mRNA level and a parallel 50% decrease in relative rate of LPL synthesis. In contrast, fasting of 1 day in EPI adipocytes decreased total LPL activity by 47% but did not affect LPL mRNA level or relative rate of LPL synthesis. A decrease in overall protein synthesis contributed to the decreased LPL activity after 1 day fasting both in EPI and MES adipocytes. In MES adipocytes the decrease in LPL activity, LPL mRNA and LPL synthesis were comparable, but in EPI adipocytes the changes in LPL activity were substantially larger than the changes in LPL mRNA level and LPL synthesis. Therefore, fasting decreased fat cell size, LPL activity, LPL mRNA level and relative rate of LPL synthesis in rats, and these effects were more marked in the MES adipocytes. These results clearly demonstrate the regional variations in the metabolic response of adipose tissue and LPL functions to fasting.
Adipose Tissue;Adipocytes;Fasting;Lipoprotein Lipase;Rat;
 Cited by
The Effects of Fasting and Grazing on Na-glucose Cotransporter-1 (SGLT-1) Gene Expression of Rectal Epithelia in Beef Cattle,Kozakai, Takaharu;Imura, K.;Nakajima, K.;Sakanoue, S.;Watanabe, N.;

Asian-Australasian Journal of Animal Sciences, 2009. vol.22. 2, pp.232-237 crossref(new window)
Bergo, M., G. Olivecrona and T. Olivecrona. 1996. Forms of lipoprotein lipase in rat tissues: in adipose tissue the proportion of inactive lipase increases on fasting. Biochem. J. 313:893-898.

Choi, S. H., K. W. Lim, H. G. Lee, Y. J. Kim and M. K. Song. 2007. Supplementation effects of C18:2 or C18:3 rich-oils on formations of CLA and TVA, and lipogenesis in adipose tissues of sheep. Asian-Aust. J. Anim. Sci. 20:1417-1423.

Chomzczynski, P. and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidium thiocyanate-phenolchlorofrom extraction. Anal. Biochem. 162:156-159.

Cruz, M. L. and D. H. Williamson. 1992. Refeeding meal-fed rats increase lipoprotein lipase activity and deposition of dietary $(^{14}C)$ lipid in white adipose tissue and decreases oxidation to $^{14}CO_2$. Biochem. J. 285:771-778.

Cryer, A. 1981. Tissue lipoprotein lipase activity and its action in lipoprotein metabolism. Int. J. Biochem. 13:525-541. crossref(new window)

Digirolamo, M., S. Mendlinger and J. A. Fertig. 1971. Simple method determines fat cell size and number in four mammalian species. Am. J. Physiol. 221:850-858.

Doolittle, M. H., O. Ben-Zeev, J. Elovson, D. Wartin and T. G. Kirchgessner. 1990. The response of lipoprotein lipase to feeding and fasting. Evidence for post translational regulation. J. Biol. Chem. 265:4570-4577.

Eckel, R. H. 1989. Lipoprotein lipase: a multifunctional enzyme relevant to common metabolic disease. New Eng. J. Med. 320:1060-1068. crossref(new window)

Folch, J., M. Lee and G. H. Sloane-Stanely. 1957. A simple method for the isolation and purification of total lipid from animal tissue. J. Biol. Chem. 226:497-509.

Fried, S. K. and J. G. Kral. 1987. Sex differences in regional distribution of fat cell size and lipoprotein lipase activity in morbidly obese patients. Int. J. Obes. 11:129-140.

Fried, S. K. and R. Zechner. 1989. Effects of catechin/tumor necrosis factor on human adipose tissue lipoprotein lipase activity, mRNA levels, and biosynthesis. J. Lipid Res. 30:1917-1923.

Fried, S. K., N. Velazquez and J. Nobel. 1990. Nutrition-induced variations in responsiveness to insulin effects on lipoprotein lipase activity in isolated rat fat cells. J. Nutr. 120:1087-1095.

Gray, J. M. and G. N. Wade. 1980. Cytoplasmic estrogen, but not progestin, binding sites in male rat adipose tissue. Endocrine. 239:E237-E241.

Hirsch, J. and E. Gallan. 1968. Methods for the determination of adipose cell size in man and animals. J. Lipid Res. 9:110-119.

Iverius, P. and J. D. Brunzell. 1985. Human adipose tissue lipoprotein lipase: changes with feeding and relation to postheparin plasma enzyme. Am. J. Physiol. E249:E109-E114.

Ladu, M. J., H. Kapas and W. K. Palmer. 1991. Regulation of lipoprotein lipase in adipose tissue and muscles during fasting. Biochim. Biophys. Acta. 878:330-337.

Lee, J. J., P. J. Smith and S. K. Fried. 1998. Mechanisms of decreased lipoprotein lipase activity in adipocytes of starved rats depend on duration of starvation. J. Nutr. 128:940-946.

Lien, T. F. and D. F. Jan. 2003. Enzyme activities related to lipid metabolism in the liver and adipose tissue of Tsaiya ducks under fasting and ad libitum feeding conditions. Asian-Aust. J. Anim. Sci. 16:403-408.

Liu, R., Y. Wang, D. Sun, Y. Yu and Y. Zhang. 2006. Association between polymorphisms and lipoprotein lipase gene and chicken fat deposition. Asian-Aust. J. Anim. Sci. 19:1409-1414.

Llado, I., A. Pons and A. Palou. 1999. Effect of fasting on lipoprotein lipase activity in different depots of white and brown adipose tissue in diet-induced overweight rats. J. Nutr. Biochem. 10:609-614. crossref(new window)

Oliver, J. D. and M. P. Rogers. 1993. Stimulation of lipoprotein lipase synthesis by refeeding, insulin and dexamethasone. Biochem. J. 292:525-530.

Panarotto, D., J. Poisson, G. Devroede and P. Maheux. 2000. Lipoprotein lipase steady-state and mRNA levels are lower in human omental versus subcutaneous abdominal adipose tissue. Metabolism. 49:1224-1227. crossref(new window)

Pedersen, S. B., J. D. Borglum, T. Moller-Petersen and B. Richelsen. 1992. Characterization of nuclear corticosteroid receptors in rat adipocytes. Regional variations and modulatory effects of hormones. Biochem. Biophys. Acta. 1134:303-308. crossref(new window)

Rebuffe-Scrive, M., U. A. Walsh, B. Mcewen and J. Rodin. 1992. Effects of chronic stress and exogenous glucocorticoids on regional fat distribution and metabolism. Physiol. Behav. 52:583-590. crossref(new window)

Rodbell, M. 1964. The metabolism of isolated fat cells. I. Effects of hormones on glucose metabolism and lipolysis. J. Biol. Chem. 239:375-380.

Semb, H. and T. Olivecrona. 1986. Lipoprotein lipase in guinea pig tissue: molecular size and rates of synthesis. Biochim. Biophys. Acta. 878:330-337.

Semb, H. and T. Olivecrona. 1989. Two different mechanisms are involved in the nutritional regulation of lipoprotein lipase in guinea pig adipose tissue. Biochim. J. 262:505-511.

Sponarova, J., K. J. Mustard, O. Horkova, P. Flachs, M. Rossmeisl, P. Brauner, K. Bardova, M. Thomason-Hughes, R. Braunerova, P. Janovska, D. G. Hardie and J. Kopecky. 2005. Involvement of AMP-activated protein kinase in fat depot-specific metabolic changes during starvation. FEBS Letters. 579:6105-6110. crossref(new window)

Sugden, M. C., M. J. Holness and R. M. Howard. 1993. Changes in lipoprotein lipase activities in adipose tissue, heart and skeletal muscle during continuous or interrupted feeding. Biochem. J. 292:113-119.

Sugden, M. C., R. M. Grimshaw and M. J. Holness. 1999. Caloric restriction leads to regional specialization of adipocyte function in the rat. Biochim. Biophys. Acta. 1437:202-213.

Tchernof, A., C. Belanger, A. S. Morisset, C. Richard, J. Mailloux, P. Laberge and P. Dupont. 2006. Regional differences in adipose tissue metabolism in women. Minor effect of obesity and body fat distribution. Diabetes. 55:1353-2006. crossref(new window)

Vannier, C., D. Gaillard, P. Grimaldi, E. Z. Amri, P. Djian, C. Cermolacce, C. Forest, J. Etienne, R. Negrel and G. Ailhaud. 1985. Adipose conversion of OB17 cells and hormone-related events. Int. J. Obesity. 9 (supple 1):41-53.

Wu, G., G. Olivecrona and T. Olivecrona. 2003. The distribution of lipoprotein lipase in rat adipose tissue. J. Biol. Chem. 278:11925-11930. crossref(new window)

Zechner, R. 1990. Rapid and simple isolation procedure for lipoprotein lipase from human milk. Biochim. Biophys. Acta. 1044:20-25.