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Effect of Galacto-mannan-oligosaccharides or Chitosan Supplementation on Cytoimmunity and Humoral Immunity in Early-weaned Piglets
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 Title & Authors
Effect of Galacto-mannan-oligosaccharides or Chitosan Supplementation on Cytoimmunity and Humoral Immunity in Early-weaned Piglets
Yin, Y.-L.; Tang, Z.R.; Sun, Z.H.; Liu, Z.Q.; Li, T.J.; Huang, R.L.; Ruan, Z.; Deng, Z.Y.; Gao, B.; Chen, L.X.; Wu, G.Y.; Kim, S.W.;
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Immunomodulatory feed additives might offer alternatives to antimicrobial growth promoters in pig production. This experiment was designed to determine the effects of dietary galacto-mannan-oligosaccharide (GMOS) and chitosan oligosaccharide (COS) supplementation on the immune response in early-weaned piglets. Forty 15-day-old piglets (DurocLandraceYorkshire) with an average live body weight of were weaned and randomly assigned to 4 treatment groups that were fed maize-soybean meal diets containing either basal, 110 mg/kg of lincomycin, 250 mg/kg of COS or 0.2% GMOS, respectively, over a 2-week period. Another six piglets of the same age were sacrificed on the same day at the beginning of the study for sampling, in order to obtain baseline values. Interleukin (IL)-1gene expression in peripheral blood monocytes, jejunal mucosa and lymph nodes, as well as serum levels of IL-1 IL-2 and IL-6, IgA, IgG, and IgM, were evaluated for 5 pigs from each group at 15 and 28 days of age. The results indicate that weaning stress resulted in decreases in serum antibody and cytokine levels. Dietary supplementation with GMOS or COS enhanced (p<0.05) IL-1gene expression in jejunal mucosa and lymph nodes, as well as serum levels of IL-1 IL-2, IL-6, IgA, IgG and IgM compared to supplementation with lincomycin. These findings suggest that GMOS or COS may enhance the cell-mediated immune response in early-weaned piglets by modulating the production of cytokines and antibodies, which shows that GMOS or COS have different effects than the antibiotic on animal growth and health.
Oligosaccharides;Interleukin-1;-2 and -6;Gene Expression;Immune Function;Piglets;
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Arthington, J. D., L. R. Corah and F. Blecha. 1996. Effect of molybdenum-induced copper deficiency on acute-phase protein concentrations, superoxide dismutase activity, leukocyte numbers, and lymphocyte proliferation in beef heifers inoculated with bovine herpesvirus-1. J. Anim. Sci. 74:211-217. crossref(new window)

Bach Knudsen, K. E. 2001. Development of antibiotic resistance and options to replace antimicrobials in animal diets. Proc. Nutr. 60:291-299. crossref(new window)

Bassaganya-Riera, J. K. Hontecillas-Magarzo, M. Bregendahl, J. Wannemuehler and D. R. Zimmerman. 2001. Effects of dietary conjugated linoleic acid in nursery pigs of dirty and clean environments on growth, empty body composition, and immune competence. J. Anim. Sci. 79:714-721. crossref(new window)

Bezirtzoglou, E., M. B. Romond and C. Romond. 1989. Modulation of Clostridium perfringens intestinal colonization in infants delivered by caesarean section. Infection. 17:232-236. crossref(new window)

Blok, M. C., H. A. Vahl, A. E. de Lange, G.van de Braak, M. Hemke and M. Hessing. 2002. Nutrition and health of the gastrointestinal tract (1st ed). Netherlands,USA.

Davis, M. E., C. V. Maxwell, D. C. Brown, B. Z. de Rodas, Z. B. Johnson, E. B. Kegley, D. H. Hellwig and R. A. Dvorak. 2002. Effect of dietary mannan oligosaccharides and (or) harmacological additions of copper sulfate on growth performance and immunocompetence of weanlingand growing/finishing pigs. J. Anim. Sci. 80:2887-2894. crossref(new window)

Deng, Ze-Yuan, J. W. Zhang, G. Y. Wu, Y. L. Yin, Z. Ruan, T. J. Li, W. Y. Chu, X. F. Kong, Y. M. Zhang, Y. W. Fan, R. Liu and R. L. Huang. 2007b. Dietary supplementation with polysaccharides from Semen cassiae enhances immunoglobulin production and interleukin gene expression in early-weaned piglets. J. Sci. Food Agric. 87:1868-1873. crossref(new window)

Deng, Ze-yuan, J. W. Zhang, J. Li, Y. W. Fan, S. W. Cao, R. L. Huang, Y. L. Yin, H. Y. Zhong and T. J. Li. 2007. Effect of polysaccharides of cassiae seeds on the intestinal microflora of piglets. Asia Pac. J. Clin. Nutr. 16(Suppl 1):143-147.

Fuller, R. 1992. History and development of probiotics. In: Probiotics Scientific Basis. (Ed. R. Fuller), pp. 1-8. Chapman and Hall, London.

Gomez, G. G., O. Philips, R. A. Goforth. 1998. Effect of immunoglobulin source on survival, growth and haematological and immunological variables in pigs. J. Anim. Sci. 76:1-7.

Guigoz, R.Y. F., C. G. Perruisseau, I. Rochat and E. J. Schiffrin. 2002. Effects of oligosaccharide on the faecal flora and nonspecific immune system in elderly people. Nutr. Res. 22:13-25. crossref(new window)

Hicks, T. A., J. McGlone, C. S. Whisnant, H. G. Kattesh and R. L. Norman. 1998. Behavioral, endocrine, immune and performance measures for pigs exposed to acute stress. J. Anim. Sci. 76:474-483. crossref(new window)

Huang, Rui-lin, Yin Yu-Long, Mei-xiang Li, Guo-yao Wu, Tie-jun Li, Li-li Li, Cheng-bo Yang, Jun Zhang, Bin Wang, Zhe-yuan Deng, Yong-gang Zhang, Zhi-ru Tang, Ping Kang, Yu-ming Guo. 2007. Dietary oligochitosan supplementation. enhances immune status of broilers. J. Sci. Food and Griculture. 87:153-159. crossref(new window)

Kelly, S., C. M. Swanson, M. Grieshop, A. Elizabeth, M. Flickinger, R. Neal, G. C. Merchen and J. Fahey. 2002. Effects of supplemental fructooligosaccharides and mannanoligosaccharides on colonic microbial populations, immune function and fecal odor components in the canine. J. Nutr. 132: 1717S-1719S. crossref(new window)

Kitazawa, H., T. Ino, Y. Kawai, T. Itoh and T. Saito. 2002. A novel immunostimulation aspect of Lactobacillus gaseri, induction of 'Gasserokine' as chemoattractants for macrophages. J. Food. Microbiol. 77:29-38. crossref(new window)

Kong, X. F., Y. L. Hu, Y. L. Yin, G. Y. Wu, R. Rui, D. Y. Wang and C. B. Yang. 2006. Chinese herbal ingredients are effective immune stimulators for chickens infected with the Newcastle Disease virus. Poult. Sci. 85:2169-2175. crossref(new window)

Kong, X.-F., G. Y. Wu, Y. L. Yin, H. J. Liu, F. G. Yin, T. J. Li, R. L. Huang, P. Kang, F. F. Xing, M. Z. Fan, C. B. Yang and Q. H. He. 2007a. Dietary supplementation with Chinese herbal ultrafine powder enhances cellular and humorl immunity in earlyweaned piglets. Livest. Sci. 108:94-98. crossref(new window)

Kong, X. F., Y. L. Yin, G. Y. Wu, Y. Q. Hou, H. J. Liu, F. G. Yin, T. J. Li, R. L. Huang, Z. Ruan, H. Xiong, Z. Y. Deng, M. Y. Xie, Y. P. Liao, L. X. Chen and S. W. Kim. 2007b. Dietary supplementation with acanthopanax senticosus extract modulates cellular and humoral immunities in weaned piglets. Asian-Aust. J. Anim. Sci. 20:1453-1461. crossref(new window)

Kong, X. F., G. Y. Wu, Y. P. Liao, Z. P. Hou, H. J. Liu, F. G. Yin, T. J. Li, R. L. Huang, Y. M. Zhang, D. Deng, P. Kang, R. X. Wang, Z. Y. Tang, C. B. Yang, Z. Y. Deng, H. Xiong, W. Y. Chu, Z. Yuan, M.Y. Xie and Y. L. Yin. 2007c. Effects of Chinese herbal ultra-fine powder as a dietary additive on growth performance, serum metabolites and intestinal health in early-weaned piglets. Livest. Sci. 108:272-275. crossref(new window)

Li, P., Y. L. Yin, D. Li, S. W. Kim and G. Wu. 2007a. Amino acids and immune function. Br. J. Nutr. 98:237-252. crossref(new window)

Li, L.-L., Y.-L. Yin, Y.-H. Liu, D.-X. Hou, Z.-P. Hou, C.-B. Yang, X.-J. Yang. 2007b. Intramuscular administration of zinc metallothionein to preslaughter stressed pigs improves antioxidative status and porl quality. Asian-Aust. J. Anim. Sci. 20:761-767. crossref(new window)

Monsan, P. 1950. Oligosaccharide feed additives. In: Biotechnology in animal feeds and animal feeding (Ed. R. J. Wallace and A. Chesson), pp. 232. VCH, New York.

Morita, H., F. He, T. Fuse, A. C. Ouwehand, H. Hashimoto, M. Hosoda, K. Mizumachi and J. Kurisaki. 2002. Adhesion of lactic acid bacteria to Caco-2 cells and their effect on cytokine secretion. Microbiol. Immunol. 46:293-297. crossref(new window)

National Research Council (NRC). 1998. Nutrient requirements for swine. 10th ed. Washington, DC In: National Academy Press.

Orban, J. I., J. A. Patterson, O. Adeola, A. L. Sutton and G. N. Richards. 1997. Growth performance and intestinal microbial population of growing pigs fed diets containing sucrose thermal oligosaccharide caramel. J. Anim. Sci. 75:170-175 crossref(new window)

Pierre, F., P. Perrin, M. Champ, F. Bornet, K. Meflah and J. Menanteau. 1997. Short-chain fructo-oligosaccharides reduce the occurrence of colon tumors, and develop gut-associated lymphoid tissue in Min mice. Cancer. Res. 57:225-228

Pie, S., J. P. Lalles, F. Blazy, J. Laffitte, B. Seve and I. P. Oswald. 2004. Weaning is associated with an upregulation of expression of inflammatory cytokines in the intestine of piglets. J. Nutr. 134:641-647 crossref(new window)

Ruan, Z., G. Zang, Y.-L. Yin, T.-J. Li, R.-L. Huang, S. W. Kim, G. Y. Wu and Z. Y. Deng. 2007. Dietary requirement of true digestible phosphorus and total calcium for growing pigs. Asian-Aust. J. Anim. Sci. 20:1236-1242 crossref(new window)

Savage, T. F., E. I. Zakrewska, Jr. and J. R. Andreasen. 1997. Oligosaccharide supplemented diets to poults on performance and the morphology of the small intestine. Poult. Sci. 76(Suppl. 1), 139 (Abstr)

Sekine, K., T. Toida, M. Saito, M. Kuboyama, T. Kawashima and Y. Hashimoto. 1985. A new morphologically characterized cellwall preparation (whole peptidoglycan) from Bifidobacterium infantis with a higher efficacy on the regression on an established tumor in mice. Cancer. Res. 45:1300-1307

Spring, P., C. Wenk, K. A. Dawson and K. E. Newman. 2000. The effects of dietary mannanoligosaccharides on cecal parameters and the concentrations of enteric bacteria in the ceca of salmonella-challenged broiler chicks. Poult. Sci. 79:205-211 crossref(new window)

Tang, Z. Y., Y. L. Yin, C. N. Nyachoti, R. L. Huang, T. J. Li, C. B. Yang, X. J. Yang, J. H. Gong, J. Peng, D. S. Qi, J. J. Xing, Z. H. Sun and M. Z. Fan. 2005. Effect of dietary supplementation of chitosan and galacto-mannan-oligosaccharide on serum parameters and the insulin-like growth factor-I mRNA expression in early-weaned piglets. Domest. Anim. Endocri. 28:430-441 crossref(new window)

Tokunaga, T., T. Oku and N. Hosoya. 1989. Utilization and excretion of a new sweetener, fructoolisaccharide (Neosugar) in rats. J. Nutr. 119:45-53

Touchette, K. J., J. A. Carroll, G. L. Allee, R. L. Matteri, C. J. Dyer, L. A. Beausang and M. E. Zannelli. 2002. Effect of spray-dried plasma and lipopolysaccharide exposure on weaned pigs: I. Effects on the immune axis of weaned pigs. J. Anim. Sci. 80:494-501 crossref(new window)

Van, H. E., M. T. Coffey and J. W. Spears. 1996. Effects of immune challenge, dietary energy density, and source of energy on performance and immunity in weanling pigs. J. Anim. Sci. 74:2431-2440 crossref(new window)

Van Loo, J., J. Cummings and N. Delzenne. 1999. Functional food properties of non-digestible oligosaccharides: a consensus report from the ENDO project. Br. J. Nutr. 81:121-132 crossref(new window)

White, L. A., M. C. Newman, G. L. Cromwell, M. D. Lindemann. 2002. Brewers dried yeast as a source of mannan oligosaccharides for weanling pigs. J. Anim. Sci. 80:2619-2628

Wu, G. 1995. Urea synthesis in enterocytes of developing pigs. Biochem. J. 312:717-723 crossref(new window)

Wu, G. 1996. Effects of concanavalin A and phorbol myristate acetate on glutamine metabolism and proliferation of porcine intestinal intraepithelial lymphocytes. Comp. Biochem. Physiol. A. 114:363-368 crossref(new window)

Xin, Y. H., Z. W. Yi and R. X Zhi. 2000. New feedstuff additivechitosan. Feed. Res. (China) 8:18-19

Yang, F., J. J. Wang, X. J. Li, T. Y. Ying, S. Y. Qiao, D. F. Li and G. Wu. 2007. Two-dimensional gel electrophoresis and mass spectrometry analysis of interactions between Lactobacillus Fermentum I5007 and intestinal epithelial cells. Electrophoresis 28:4330-4339 crossref(new window)

Yasunori, M. and K. Yoshiyuki. 2004. Effects of various lowmolecular-weight chitosans are due to increased natural killer activity of intestinal intraepithelial lymphocytes in sarcoma 180-bearing mice. J. Nutr. 134:945-950 crossref(new window)

Yoshida, T., R. Kruger and V. Inglish. 1995. Augmentation of nonspecific protection in African catfish by the long-term oral administration of immunostimulants. J. Fish. Dis. 18:195-198 crossref(new window)