JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Expression Analysis of miRNAs in Porcine Fetal Skeletal Muscle on Days 65 and 90 of Gestation
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Expression Analysis of miRNAs in Porcine Fetal Skeletal Muscle on Days 65 and 90 of Gestation
Chen, Jian-hai; Wei, Wen-Juan; Xiao, Xiao; Zhu, Meng-Jin; Fan, Bin; Zhao, Shu-Hong;
  PDF(new window)
 Abstract
MiRNAs (microRNAs) are a class of small non-coding RNA molecules of ~21 nucleotides that down- regulate the expression of target genes at post-transcriptional level. In this study, we first accomplished a preliminary scan of miRNA expression using 65 and 90 day fetal pig skeletal muscle samples by microarray hybridization, and 34 miRNAs showed strong positive signals. Five of these miRNAs were selected for further investigation by real-time RT-PCR. The statistical analyses indicated that three miRNAs exhibited significant differential expression (p<0.05) during porcine muscle development from 65 to 90 days of gestation, e.g., miR-24 and miR-424 were down-regulated while miR-133a was up-regulated. Multi-tissue RT-PCR was performed to detect the expression patterns of the five miRNA precursors. The results showed that most of these precursor miRNAs were ubiquitously expressed in different porcine tissues.
 Keywords
Pig;MicroRNA;Expression;Skeletal Muscle;
 Language
English
 Cited by
1.
Effects of Castration on Androgen Receptor, IGF-I Ea, MGF and Myostatin Gene Expression in Skeletal Muscles of Male Pigs,;;;;;;;;

Asian-Australasian Journal of Animal Sciences, 2009. vol.22. 8, pp.1069-1077 crossref(new window)
2.
Effect of Thyroid Hormone on the Gene Expression of Myostatin in Rat Skeletal Muscle,;;;;;

Asian-Australasian Journal of Animal Sciences, 2009. vol.22. 2, pp.275-281 crossref(new window)
 References
1.
Ambros, V. 2003. MicroRNA pathways in flies and worms: growth, death, fat, stress, and timing. Cell 113:673-676. crossref(new window)

2.
Bartel, D. P. 2004. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116:281-297. crossref(new window)

3.
Bernstein, E., S. Y. Kim, M. A. Carmell, E. P. Murchison, H. Alcorn, M. Z. Li, A. A. Mills, S. J. Elledge, K. V. Anderson and G. J. Hannon. 2003. Dicer is essential for mouse development. Nat Genet. 35:215-217. crossref(new window)

4.
Cagnazzo, M., M. F. te Pas, J. Priem, A. A. de Wit, M. H. Pool, R. Davoli and V. Russo. 2006. Comparison of prenatal muscle tissue expression profiles of two pig breeds differing in muscle characteristics. J. Anim. Sci. 84:1-10.

5.
Chen, J. F., E. M. Mandel, J. M. Thomson, Q. Wu, T. E. Callis, S. M. Hammond, F. L. Conlon and D. Z. Wang. 2006. The role of microRNA-1 and microRNA-133 in skeletal muscle proliferation and differentiation. Nat. Genet. 38:228-233. crossref(new window)

6.
Chul, W. K., K. T. Chang, Y. H. Hong, W. Y. Jung, E. J. Kwon, K. K. Cho, K. H. Chung, B. W. Kim, J. G. Lee, J. S. Yeo, Y. S. Kang and Y. K. Joo. 2005. cDNA microarray analysis of the gene expression profile of wwine muscle. Asian-Aust. J. Anim. Sci. 18:1080-1087.

7.
Esau, C., X. Kang, E. Peralta, E. Hanson, E. G. Marcusson, L. V. Ravichandran, Y. Sun, S. Koo, R. J. Perera, R. Jain, N. M. Dean, S. M. Freier, C. F. Bennett, B. Lollo and R. Griffey. 2004. MicroRNA-143 regulates adipocyte differentiation. J. Biol. Chem. 279:52361-52365. crossref(new window)

8.
Eun, C., W. Y. Jung, E. J. Kwon, D. H. Park, K. H. Chung, K. K. Cho and C. W. Kim. 2007. Cloning and expression analysis of the subunit of porcine prolyl 4-hydroxylase. Asian-Aust. J. Anim. Sci. 20:1655-1661.

9.
Giraldez, A. J., R. M. Cinalli, M. E. Glasner, A. J. Enright, J. M. Thomson, S. Baskerville, S. M. Hammond, D. P. Bartel and A. F. Schier. 2005. MicroRNAs regulate brain morphogenesis in zebrafish. Sci. 308:833-83. crossref(new window)

10.
Kim, H. J., X. S. Cui, E. J. Kim, W. J. Kim and N. H. Kim. 2006. New porcine microRNA genes found by homology search. Genome. 49:1283-1286. crossref(new window)

11.
Kim, S. M., M. Y. Park, K. S. Swo, D. H. Yoon, H-G. Lee, Y. J. Choi and S. H. Kim. 2006. Analysis of differentially expressed proteins in bovine longissimus dorsi and biceps femores muscles . Asian-Aust. J. Anim. Sci. 19:1496-1502.

12.
Lee, R. C., R. L. Feinbaum and V. Ambros. 1993. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell. 75:843-854. crossref(new window)

13.
Nam-Kuk, K., J-H. Lim, M-J. Song, O-H. Kim, B-Y. Park, M-J. Kim, I-H. Hwang and C-S. Lee. 2007. Developmental proteomic profiling of porcine skeletal muscle during postnatal development. Asian-Aust. J. Anim. Sci. 20:1612-1617.

14.
Olsen, P. H. and V. Ambros. 1999. The lin-4 regulatory RNA controls developmental timing in Caenorhabditis elegans by blocking LIN-14 protein synthesis after the initiation of translation. Dev. Biol. 216:671-680. crossref(new window)

15.
Sawera, M., J. Gorodkin, S. Cirera and M. Fredholm. 2005. Mapping and expression studies of the mir17-92 cluster on pig chromosome 11. Mamm Genome. 16:594-598. crossref(new window)

16.
Shi, R. and V. L. Chiang. 2005. Facile means for quantifying microRNA expression by real-time PCR. Bio Techniques. 39:519-525.

17.
Swatland, H. J. 1994. Structure and development of meat animals and poultry. Technomic Publishing Company. Inc. Pennsylvania. pp. 495-563.

18.
Tang, Z. L., Y. Li, P. Wan, X. P. Li, S. H. Zhao, B. Liu, B. Fan, M. J. Zhu, M. Yu and K. Li. 2007. Long SAGE analysis of skeletal muscle at three prenatal stages in Tongcheng and Landrace pigs. Genome Biol. 8(6):R115.1-R115.18. crossref(new window)

19.
Te Pas, M. F., A. A. C. de Wit, J. Priem, M. Cagnazzo, R. Davoli, V. Russo and M. H. Pool. 2005. Transcriptome expression profiles in prenatal pigs in relation to myogenesis. J. Muscle Res. Cell Motil. 26:157-165. crossref(new window)

20.
Wernersson, R., M. H. Schierup, F. G. Jorgensen, J. Gorodkin, F. Panitz, H. H. Staerfeldt, O. F. Christensen, T. Mailund, H. Hornshoj, A. Klein, J. Wang, B. Liu, S. Hu, W. Dong, W. Li, G. K. Wong, J. Yu, C. Bendixen, M. Fredholm, S. Brunak, H. Yang and L. Bolund. 2005. Pigs in sequence space: a 0.66X coverage pig genome survey based on shotgun sequencing. BMC Genomics. 6(1):70. crossref(new window)

21.
Xu, P., M. Guo and B. A. Hay. 2004. MicroRNAs and the regulation of cell death. Trends Genet. 20:617-624. crossref(new window)

22.
Zhao, S. H., D. Kuhar, J. K. Lunney, H. D. Dawson, C. Guidry, J. Uthe, S. Bearson, J. Recknor, D. Nettleton and C. K. Tuggle. 2006. Gene expression profiling in salmonella choleraesuisinfected porcine lung using a long oligonucleotide microarray. Mamm Genome. 17:777-789. crossref(new window)

23.
Zhao, S. H., D. Nettleton, W. Liu, C. Fitzsimmons, C. W. Ernst, N. E. Raney and C. K. Tuggle. 2003. Complementary DNA macroarray analyses of differential gene expression in porcine fetal and postnatal muscle. J. Anim. Sci. 81:2179-2188.

24.
Zhao, S. H., J. Yu, B. Liu, B. Fan, M. J. Zhu, T. A. Xiong, M. Yu and K. Li. 2005. The porcine FBXO32 gene: map assignment, SNP detection and tissue expression. Anim. Genet. 36:451-452. crossref(new window)