JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Effects of Cell Status of Bovine Oviduct Epithelial Cell (BOEC) on the Development of Bovine IVM/IVF Embryos and Gene Expression in the BOEC Used or Not Used for the Embryo Culture
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effects of Cell Status of Bovine Oviduct Epithelial Cell (BOEC) on the Development of Bovine IVM/IVF Embryos and Gene Expression in the BOEC Used or Not Used for the Embryo Culture
Jang, H.Y.; Jung, Y.S.; Cheong, H.T.; Kim, J.T.; Park, C.K.; Kong, H.S.; Lee, H.K.; Yang, B.K.;
  PDF(new window)
 Abstract
The objective of this study was to investigate the effects of cell status of BOEC on development of bovine IVM/IVF embryos and gene expression in BOEC before or after culturing of embryos. The developmental rates beyond morula stage in the BOEC co-culture group was significantly higher than in the control group (p<0.05). In particular, blastocyst production in the BOEC co-culture group (28.3%) was dramatically increased compared with the control group (7.2%). In the in vitro development of bovine IVM/IVF embryos according to cell status, the developmental rates beyond morula stage in the primary culture cell (PCC) co-culture group were the highest of all experimental groups. Expression of genes related to growth (TGF- EGF and IGFBP), apoptosis (Bax, Caspase-3 and p53) and antioxidation (CuZnSOD, MnSOD, Catalase and GPx) in different status cells of BOEC for embryo culture was detected by RT-PCR. While EGF gene was detected in isolated fresh cells (IFC) and PCC, TGF- and IGFBP were found in IFC or PCC after use in the embryo culture, respectively. Caspase-3 and Bax genes were detected in all experimental groups regardless of whether the BOEC was used or not used in the embryo culture. However, p53 gene was found in IFC of both conditions for embryo culture and in frozen/thawed culture cells (FPCC) after use in the embryo culture. Although antioxidant genes examined were detected in all experimental groups before using for the embryo culture, these genes were not detected after use. This study indicated that the BOEC co-culture system used for in vitro culture of bovine IVF embryos can increase the developmental rates, and cell generations and status of BOEC might affect the in vitro development of bovine embryos. The BOEC monolayer used in the embryo culture did not express the growth factors (TGF- and EGF) and enzymatic antioxidant genes, thereby improving embryo development in vitro.
 Keywords
Bovine Oviduct Epithelial Cell;Bovine IVM/IVF Embryos;Growth Factor Gene;Apoptosis Gene;Antioxidant Gene;
 Language
English
 Cited by
1.
The Effect of Cryopreservation on the Mouse Embryos at Various-pronuclear Stages,;;;;;;;;

Asian-Australasian Journal of Animal Sciences, 2009. vol.22. 2, pp.174-180 crossref(new window)
2.
Stage-specific Expression of Lanosterol 14${\alpha}$-Demethylase in Mouse Oocytes in Relation to Fertilization and Embryo Development Competence,;;;;;;;;

Asian-Australasian Journal of Animal Sciences, 2009. vol.22. 3, pp.319-327 crossref(new window)
3.
Lmbr1 Expression in Early Embryo Development Stages in White Leghorn and Chinese Silky,;;;

Asian-Australasian Journal of Animal Sciences, 2009. vol.22. 1, pp.7-12 crossref(new window)
4.
Bovine Oocytes Can Be Penetrated in Modified Tris-buffered Medium,;;

Asian-Australasian Journal of Animal Sciences, 2009. vol.22. 4, pp.500-506 crossref(new window)
 References
1.
Bavister, B. D. 1992. Co-culture for embryo development: is it really necessary? Hum. Reprod. 7(10):1339-1341.

2.
Brackett, B. G., D. Bousquet, M. L. Boice, W. J. Donawick, J. F. Evans and M. A. Dressel. 1982. Normal development following in vitro fertilization in the cow. Biol. Reprod. 27(1):147-158. crossref(new window)

3.
Brackett, B. G. and G. Oliphant. 1975. Capacitation of rabbit spermatozoa in vitro. Biol. Reprod. 12:260-274. crossref(new window)

4.
Carolan, C., P. Lonergan, H. Khatir and P. Mermillod. 1996. In vitro production of bovine embryos using individual oocytes. Mol. Reprod. Dev. 45(2):145-150. crossref(new window)

5.
Carolan, C., P. Lonergan, A. Van Langendonckt and P. Mermillod. 1995. Factors affecting bovine embryo development in synthetic oviduct fluid following oocyte maturation and fertilization in vitro. Theriogenol. 43(6):1115-1128. crossref(new window)

6.
Chomczynski, P. and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162(1):156-159.

7.
de Lamirande, E. and C. Gagnon. 1992. Reactive oxygen species and human spermatozoa. I. Effect on the motility of intact spermatozoa and on sperm axonemes. J. Androl. 13:368-378.

8.
Donnay, I., A. Van Langendonckt, P. Auquier, B. Grisart, A. Vansteenbrugge, A. Massip and F. Dessy. 1997. Effects of coculture and embryo number on the in vitro development of bovine embryos. Theriogenol. 47(8):1549-1561. crossref(new window)

9.
Gardner, D. K. and M. Lane. 2005. Ex vivo early embryo development and effects on gene expression and imprinting. Reprod. Fertil. Dev. 17:361-370. crossref(new window)

10.
Guerin, P., E. I. Mouatassim and Y. Menezo. 2001. Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings. Hum. Reprod. Update. 7:175-189. crossref(new window)

11.
Halliwell, B. and O. I. Aruoma. 1991. DNA damage by oxygenderived species. Its mechanism and measurement in mammalian systems. FEBS. Lett. 281:9-19. crossref(new window)

12.
Harvey, M. B., M. Y. Arcellana-Panlilio, X. Zhang, G. A. Schultz and A. J. Watson. 1995. Expression of genes encoding antioxidant enzymes in preimplantation mouse and cow embryos and primary bovine oviduct cultures employed for embryo coculture. Biol. Reprod. 53(3):532-540. crossref(new window)

13.
Jang, H. Y., H. S. Kong, K. D. Choi, G. J. Jeon, B. K. Yang, C. K. Lee and H. K. Lee. 2005. Effect of melatonin on gene expression of IVM/IVF porcine embryos. Asian-Aust. J. Anim. Sci. 18:17-21.

14.
Jurisicova, A. and B. M. Acton. 2004. Deadly decisions: the role of genes regulating programmed cell death in human preimplantation embryo development. Reprod. 128:281-291. crossref(new window)

15.
Johnson, M. H. and M. H. Nasr-Esfahani. 1994. Radical solutions and cultural problems: could free oxygen radicals be responsible for the impaired development of preimplantation mammalian embryos in vitro. Bioessays 16(1):31-38. crossref(new window)

16.
Kim, D. H., D. S. Ko, H. C. Lee, H. J. Lee, W. I. Park and S. S. Kim. 2004. Comparison of maturation, fertilization, development and gene expression of mouse oocytes grown in vitro and in vivo. J. Assist. Reprod. Genet. 21:233-240.

17.
Kim, J. Y., M. C. Park, H. Park, Y. S. Park, H. Park, J. H. Lee and J. M. Kim. 2007. Addition of macromolecules to PZM-3 culture medium on the development and hatching of in vitro porcine embryos. Asian-Aust. J. Anim. Sci. 20:1820-1826.

18.
Kreysing, U., T. Nagai and H. Niemann. 1997. Male-dependent variability of fertilization and embryo development in two bovine in vitro fertilization systems and the effects of casein phosphopeptides (CPPs). Reprod. Fertil. Dev. 9(4):465-474. crossref(new window)

19.
Lee, Y. S., S. A. Ock, S. K. Cho, B. G. Jeon, T. Y. Kang, S. Y. Choe and G. J. Rho. 2007. Effect of donor cell types and passages on preimplantation development and apoptosis in porcine cloned embryos. Asian-Aust. J. Anim. Sci. 20:711-717.

20.
Li, J., C. A. Bombeck, S. Yang, Y. M. Kim and T. R. Billiar. 1999. Nitric oxide suppresses apoptosis via interrupting caspase activation and mitochondrial dysfunction in cultured hepatocytes. J. Biol. Chem. 274:17325-17333. crossref(new window)

21.
Liu, L. P. S., S. T. H. Chan, P. C. Ho and W. S. B.Yeung. 1998. Partial purification of embryotrophic factors from human oviductal cells. Hum. Reprod. 13:1613-1619. crossref(new window)

22.
Muller, M., S. Wilder, D. Bannasch, D. Israeli, K. Lehlbach, M. Li-wever, S. L. Friedman, P. R. Galle, M. Stremmel, M. Oren and P. H. Krammer. 1998. p53 activates the CD95 (APO-1/Fas) gene in response to DNA damage by anticancer drugs. J. Exp. Med. 188:2033-2045. crossref(new window)

23.
Nasr-Esfahani, M. H., N. J. Winston and M. H. Johnson. 1992. Effects of glucose, glutamine, ethylenediaminetetra acetic acid and oxygen tension on the concentration of reactive oxygen species and on development of the mouse preimplantation embryo in vitro. J. Reprod. Fertil. 96:219-231. crossref(new window)

24.
Orsi, N. M. and J. B. Reischl. 2006. Mammalian embryo coculture: Trials and tribulations of a misunderstood method. Theriogenol. (in press).

25.
Ouhibi, N., J. Hamidi, J. Guillaud and Y. Menezo. 1990. Coculture of 1-cell mouse embryos on different cell supports. Hum. Reprod. 5:737.

26.
Rexroad, C. E. and A. M. Powell. 1988. Coculture of ovine ova with oviductal cells in medium 199. J. Anim. Sci. 66:947.

27.
Rief, S., F. Sinowatz-Stojkovic, R. Einspanier, E. Wolf and K. Prelle. 2002. Effect of a novel co-culture system on development, metabolism and gene expression of bovine embryos produced in vitro. Reprod. 124:543-556. crossref(new window)

28.
Uberti, D., E. Yavin, S. Gil, K. R. Ayasola, N. Goldfinger and A. Rotter. 1999. Hydrogen peroxide induces nuclear translocation of p53 and apoptosis in cells of oligodendroglia origin. Mol. Brain Res. 65:167-175. crossref(new window)

29.
Vanroose, G., A. Van Soom and A. de Kruif. 2001. From co-culture to defined medium: State if the art and practical considerations. Reprod. Dom. Anim. 36:25-28. crossref(new window)

30.
Yim, S. V., K. M. Kim, S. S. Kim, J. A. Han, H. Z. Lea and Y. M. Kim. 2000. Serotonin induces apoptosis in PGT-beta pineal gland tumor cells. Jpn. J. Pharmacol. 84:71-74. crossref(new window)