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Molecular Cloning, Tissue Distribution and Segmental Ontogenetic Regulation of b0,+ Amino Acid Transporter in Lantang Pigs
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 Title & Authors
Molecular Cloning, Tissue Distribution and Segmental Ontogenetic Regulation of b0,+ Amino Acid Transporter in Lantang Pigs
Zhi, Ai-Min; Feng, Ding-Yuan; Zhou, Xiang-Yan; Zou, Shi-Geng; Huang, Zhi-Yi; Zuo, Jian-Jun; Ye, Hui; Zhang, Chang-Ming; Dong, Ze-Min; Liu, Zhun;
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Cationic amino acid transporter (HGMW-approved gene symbol SLC7A9, solute carrier family 7, member 9) plays a crucial role in amino acid nutrition. In the present study, we describe the cloning and sequencing of porcine . Based on the sequence of porcine deposited in the NCBI (National Center for Biotechnological Information), we identified a putative porcine homologue. Using rapid amplification of cDNA ends (RACE), the full-length cDNA encoding porcine was isolated. The porcine cDNA was 1,680 bp long, encoding a 487 amino acid trans-membrane protein. The predicted amino acid sequence was found to have 88.9% and 87.1% identity with human and mouse , respectively. Real-time RT-PCR indicated porcine transcripts expressed in heart, kidney, muscle and small intestine. The small intestine had the highest mRNA abundance while the muscle had the lowest (p<0.05). Along the longitudinal axis, the ileum had the highest mRNA abundance while the colon had the lowest (p<0.05). The mRNA level was highest on day 7 and 90 in the duodenum (p<0.05). It increased from day 1 to day 26 in the jejunum (p>0.05) and had the highest abundance on day 60 (p<0.05). There was, however, no difference between day 1, 7, 26, 30, 90 and 150 (p>0.05). The strongest expression appeared on day 7 in the ileum before weaning, and then decreased till day 30 but rose gradually again from day 60 to 150 (p<0.05).
Cationic Amino Acid Transporter;;SLC7A9;Ontogenetic Regulation;
 Cited by
Molecular Cloning, Tissue Distribution and Expression of Porcine y+L Amino Acid Transporter-1,;;;;;;;;

아세아태평양축산학회지, 2010. vol.23. 2, pp.272-278 crossref(new window)
Arriza, J. L., M. P. Kavanaugh, W. A. Fairman, Y. N. Wu, G. H. Murdoch, R. A. North and S. G. Amara. 1993. Cloning and expression of a human neutral amino acid transporter with structural similarity to the glutamate transporter gene family. J. Biol. Chem. 268:15329-15332.

Buddington, R. K., J. Elnif, A. A. Puchal-Gardiner and P. T. Sangild. 2001. Intestinal apical amino acid absorption during development of the pig. Am. J. Physiol. Regul. Integr. Comp. Physiol. 280:241-247. crossref(new window)

Chairoungdua, A., H. Segawa, J. Y. Kim, K.-I. Miyamoto, H. Haga, Y. Fukui, K. I. Mizoguchi, H. Ito, E. Takeda, H. Endou, Y. Kanai. 1999. Identification of an amino acid transporter associated with the cystinuria-related type II membrane glycoprotein. J. Biol. Chem. 274:28845-28848. crossref(new window)

Chaudhry, F. A., R. J. Reimer, D. Krizaj, D. Barber, J. Storm-Mathisen, D. R. Copenhagen and A. R. H. Edwards. 1999. Molecular analysis of system N suggests novel physiological roles in nitrogen metabolism and synaptic transmission farrukh a chaudhry. Cell. 99:769-780. crossref(new window)

Chillaron, J., R. Estevez, C. Mora, C. A. Wagner, H. Suessbrich, F. Lang, J. L. Gelpi, X. Testar, A. E. Busch, A. Zorzano and M. Palacin. 1996. Obligatory amino acid exchange via systems b$^{0,+}$-like and y$^+$L-like. A tertiary active transport mechanism for renal reabsorption of cystine and dibasic amino acids. J. Biol. Chem. 271:17761-17770. crossref(new window)

Chillaron, J., R. Roca, A. Valencia, A. Zorzano and M. Palacin. 2001. Heteromeric amino acid transporters: biochemistry, genetics, and physiology. Am. J. Physiol. Renal. Physiol. 281:995-1018.

de Castro, E., C. J. A. Sigrist, A. Gattiker, V. Bulliard, P. S. Langendijk-Genevaux, E. Gasteiger, A. Bairoch, N. Hulo. 2006. ScanProsite: detection of PROSITE signature matches and pro rule-associated functional and structural residues in proteins. Nucl. Acids Res. 34:362-365.

Feliubadalo, L., M. Font, J. Purroy, F. Rousaud, X. Estivill, V. Nunes, E. Golomb, M. Centola, I. Aksentijevich, Y. Kreiss, B. Goldman, M. Pras, D. L. Kastner, E. Pras, P. Gasparini, L. Bisceglia, E. Beccia1, M. Gallucci, L. D. Sanctis, A. Ponzone, G. F. Rizzoni, L. Zelante, M. T. Bassi, Jr, A. L. G. Manzoni, A. D. Grandi, M. Riboni, J. K. Endsley, A. Ballabio, G. Borsani, N. Reig, E. Fernández, R. Estevez, M. Pineda, D. Torrents, M. Camps, J. Lloberas1, A. Zorzano and M. Palacin. 1999. Non-type I cystinuria caused by mutations in SLC7A9, encoding a subunit (b$^{0,+}$AT) of rBAT. Nat.Genet. 23:52-57.

Font-Llitjos, M., L. Feliubadalo, M. Espino, R. Cleries, S. Manas, I. M. Frey, S. Puertas, G. Colell, S. Palomo, J. Aranda, J. Visa, M. Palacin and V. Nunes. 2007. Slc7a9 knockout mouse is a good cystinuria model for antilithiasic pharmacological studies. Am. J. Physiol. Renal. Physio. 293:732-740. crossref(new window)

Font, M., L. Feliubadalo, X. Estivill, V. Nunes, E. Golomb, Y. Kreiss, E. Pras, L. Bisceglia, A. P. d'Adamo, L. Zelante, P. Gasparini, M. T. Bassi, A. L. George, Jr., M. Manzoni, M. Riboni, A. Ballabio, G. Borsani, N. Reig, E. Fernandez, A. Zorzano, J. Bertran and M. Palacin. 2001. Functional analysis of mutations in SLC7A9, and genotype-phenotype correlation in non-type I cystinuria. Hum. Mol. Genet. 10:305-316. crossref(new window)

Johnson, L. R. 1997. Page 130 in Gastrointestinal Physiology, 6th ed. Mosby Inc. St. Louis, MI.

Kanai1, Y. and H. Endou1. 2001. Heterodimeric amino acid transporters: molecular biology and pathological and pharmacological relevance. Curr. Drug. Metab. 2:339-354. crossref(new window)

Kekuda, R., P. D. Prasad, Y.-J. Fei, V. Torres-Zamorano, S. Sinha, T. L. Yang-Feng, F. H. Leibach and V. Ganapathy. 1996. Cloning of the sodium-dependent, broad-scope, neutral amino acid transporter b$^{0}$ from a human placental choriocarcinoma cell line. J. Biol. Chem. 271:18657-18661. crossref(new window)

Kekuda, R., V. Torres-Zamorano, Y. J. Fei, P. D. Prasad, H. W. Li, L. D. Mader, F. H. Leibach and V. Ganapathy. 1997. Molecular and functional characterization of intestinal Na$^{(+)}$-dependent neutral amino acid transporter b$^{0}$. Am. J. Physiol. Gastrointest Liver Physiol. 272:1463-1472. crossref(new window)

Krogh, A., B. Larsson, G. V. Heijne and E. L. L. Sonnhammer. 2001. Predicting transmembrane protein topology with a hidden markov model: application to complete genomes. J. Molec. Biol. 305:567-580. crossref(new window)

Livak, K. J. and T. D. Schmittgen. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-$\Delta$CT method. Methods 25:402-408. crossref(new window)

Mastroberardino, L., B. Spindler, R. Pfeiffer, P. J. Skelly, J. Loffing, C. B. Shoemaker and F. Verrey. 1998. Amino-acid transport by heterodimers of 4F2hc/CD98 and members of a permease family. Nature 395(6699):288-291. crossref(new window)

Munck, L. K., M. L. Grondahl, J. E. Thorboll, E. Skadhauge and B. G. Munck. 2000. Transport of neutral, cationic and anionic amino acids by systems B, b$^{0,+}$, XAG, and ASC in swine small intestine. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 126:527-537. crossref(new window)

Palacin, M., R. Estevez, J. Bertran and A. Zorzano. 1998. Molecular biology of mammalian plasma membrane amino acid transporters. Physiol. Rev. 78:969-1054. crossref(new window)

Pfeiffer, R., B. Spindler, J. Loffing, P. J. Skelly, C. B. Shoemaker and F. Verrey. 1998. Functional heterodimeric amino acid transporters lacking cysteine residues involved in disulfide bond. FEBS. 439:157-162. crossref(new window)

Pfeiffer, R., J. Loffing, G. Rossier, C. Bauch, C. Meier, T. Eggermann, D. Loffing-Cueni, L. C. Kuhn and F. Verrey. 1999. Luminal heterodimeric amino acid transporter defective in cystinuria. Mol. Biol. Cell 10:4135-4147. crossref(new window)

Rajan, D. P., R. Kekuda, W. Huang, H. Wang, L. D. Devoe, F. H. Leibach, P. D. Prasad and V. Ganapathy. 1999. Cloning and Expression of a b$^{0,+}$-like amino acid transporter functioning as a heterodimer with 4F2hc instead of rBAT. A new candidate gene for cystinuria. J. Biol. Chem. 274:29005-29010. crossref(new window)

Reig, N., J. Chillaron, P. Bartoccioni, E. Fernandez, A. Bendahan, A. Zorzano, B. Kanner, M. Palacin and J. Bertran. 2002. The light subunit of system b$^{0,+}$ is fully functional in the absence of the heavy subunit. The EMBO J. 21:4906-4914. crossref(new window)

Shi-bin Yuan, Dai-wen Chen, K.-Y. Zhang and B. Yu. 2007, Effects of oxidative stress on growth performance, nutrient digestibilities and activities of antioxidative enzymes of weanling pigs. Asian-Aust. J. Anim. Sci. 20:1600-1605. crossref(new window)

Van Winkle, L. J., A. L. Campione and J. M. Gorman. 1988. Na$^+$- independent transport of basic and zwitterionic amino acids in mouse blastocysts by a shared system and by processes which distinguish between these substrates. J. Biol. Chem. 263:3150-3163.

Varoqui, H., H. Zhu, D. Yao, H. Ming and J. D. Erickson. 2000. Cloning and functional identification of a neuronal glutamine transporter. J. Biol. Chem. 275:4049-4054. crossref(new window)

Wagner, C. A., F. Lang and S. Broer. 2001. Function and structure of heterodimeric amino acid transporters. Am. J. Physiol. Cell Physiol. 281:1077-1093. crossref(new window)

Wang, J. F., T. Lundh, B. Westrm and J. E. Lindberg. 2005. The Effect of complementary access to milk replacer to piglets on the activity of brush border enzymes in the piglet small intestine. Asian-Aust. J. Anim. Sci. 18:1617-1622. crossref(new window)