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Molecular Cloning, Tissue Distribution and Segmental Ontogenetic Regulation of b0,+ Amino Acid Transporter in Lantang Pigs
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 Title & Authors
Molecular Cloning, Tissue Distribution and Segmental Ontogenetic Regulation of b0,+ Amino Acid Transporter in Lantang Pigs
Zhi, Ai-Min; Feng, Ding-Yuan; Zhou, Xiang-Yan; Zou, Shi-Geng; Huang, Zhi-Yi; Zuo, Jian-Jun; Ye, Hui; Zhang, Chang-Ming; Dong, Ze-Min; Liu, Zhun;
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Cationic amino acid transporter (HGMW-approved gene symbol SLC7A9, solute carrier family 7, member 9) plays a crucial role in amino acid nutrition. In the present study, we describe the cloning and sequencing of porcine . Based on the sequence of porcine deposited in the NCBI (National Center for Biotechnological Information), we identified a putative porcine homologue. Using rapid amplification of cDNA ends (RACE), the full-length cDNA encoding porcine was isolated. The porcine cDNA was 1,680 bp long, encoding a 487 amino acid trans-membrane protein. The predicted amino acid sequence was found to have 88.9% and 87.1% identity with human and mouse , respectively. Real-time RT-PCR indicated porcine transcripts expressed in heart, kidney, muscle and small intestine. The small intestine had the highest mRNA abundance while the muscle had the lowest (p<0.05). Along the longitudinal axis, the ileum had the highest mRNA abundance while the colon had the lowest (p<0.05). The mRNA level was highest on day 7 and 90 in the duodenum (p<0.05). It increased from day 1 to day 26 in the jejunum (p>0.05) and had the highest abundance on day 60 (p<0.05). There was, however, no difference between day 1, 7, 26, 30, 90 and 150 (p>0.05). The strongest expression appeared on day 7 in the ileum before weaning, and then decreased till day 30 but rose gradually again from day 60 to 150 (p<0.05).
Cationic Amino Acid Transporter;;SLC7A9;Ontogenetic Regulation;
 Cited by
Molecular Cloning, Tissue Distribution and Expression of Porcine y+L Amino Acid Transporter-1,Zhi, Ai-min;Zhou, Xiang-yan;Zuo, Jian-jun;Zou, Shi-geng;Huang, Zhi-yi;Wang, Xiao-lan;Tao, Lin;Feng, Ding-yuan;

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