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The Effects of Dietary Turkish Propolis and Vitamin C on Performance, Digestibility, Egg Production and Egg Quality in Laying Hens under Different Environmental Temperatures
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The Effects of Dietary Turkish Propolis and Vitamin C on Performance, Digestibility, Egg Production and Egg Quality in Laying Hens under Different Environmental Temperatures
Seven, Pinar Tatli;
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In this study, the effects of propolis and vitamin C (L-ascorbic acid) supplementation in diets were investigated on feed intake (FI), body weight (BW), body weight gain (BWG), feed conversion rate (FCR) and digestibility and on egg production and qualities (weight, mortality, shell thickness) in laying hens exposed to heat stress. A total of 150 Hyline White Leghorn, aged 42 weeks, hens was divided into five groups of 30 hens. Chicks were randomly divided into 1 positive control, 1 control and 3 treatment groups. The chicks were kept in cages in temperature-controlled rooms at for 24 h/d (positive control, Thermoneutral, TN group) or for 9 h/d from 08.00-17.00 h followed by for 15 h (control, heat stress, HS group) and fed a basal diet or basal diet supplemented with vitamin C (250 mg/kg of L- ascorbic acid/kg of diet) or two levels of propolis (2 and 5 g of ethanol extracted propolis/kg of diet). Increased FI (p<0.05) and improvement in FCR (p<0.05), hen day egg (p<0.05) and egg weight (p<0.05) were found in Vitamin C and propolis-supplemented laying hens reared under heat stress conditions. Mortality rate was higher in the control group than TN, vitamin C and propolis groups (p<0.05). Digestibility of dry matter, organic matter, crude protein and ether extract improved with increasing of both dietary vitamin C and propolis (p<0.05). Vitamin C or propolis supplementation did not affect either the percentage shape index, yolk index or haugh unit and albumen index (p>0.05). However, the egg shell thickness and egg shell weight appeared to be increased in Vitamin C and propolis groups in comparison to HS group birds (p<0.05). In conclusion, dietary supplementation of laying hens with anti-oxidants (vitamin C and propolis) can attenuate heat stress-induced oxidative damage. These positive effects were evidenced by increased growth performance and digestibility, improvement of egg shell thickness and egg weight in comparison to non-supplemented birds. Moreover, supplementation with propolis (5 g/kg diet) was the most efficient treatment.
Vitamin C;Propolis;Heat Stres;Laying Hen;
 Cited by
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아세아태평양축산학회지, 2010. vol.23. 11, pp.1482-1489 crossref(new window)
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Acikgoz, Z., B.Yucel and O. Altan. 2005. The effects of propolis supplementation on broiler performance and feed digestibility. Archiv fur Geflugelkunde 69:117-122.

AOAC. 1995. Official Methods of Analysis, 16th ed. Association of Official Analytical Chemists, Arlington, VA, 4.1-4.17.

Bains, B. S. 1996. The role of vitamin C in stress management. Missset World Poult. 12:438-442.

Banomi, A., B. M. Banomi, A. Quarantellia, A. Sabbioni and P. Superchi. 2002. The use of propolis in ducks feeding. Riv. Sci. Aliment. 31:15-28.

Borrelli, F., P. Maffia, L. Pinto, A.Ianaro, A. Russo, F.Capasso and A. Lalenti. 2002. Phytochemical compounds involved in the antiinflammatory effectof propolis extract. Fitoterapia. 73:353-36. crossref(new window)

Buhatel, T., S. Vesa, A. Dimitrin and I. Moldovan. 1983. Contribution to knowledge of the stimulative effect of propolis on piglet and pullets. Agronomic Cluj-Napoca Zootehnie Medicina Veterinara. 37:45-48

Blonska, M., J. Bronikowska, G. Pretsz, Z. P. Czuba, S. Scheller and W. Krol. 2004. Effects of ethanol extract of propolis (EEP) and flavones on inducible gene expression in J774a, 1 makrophages. J. Ethnopharmacol. 91:25-30. crossref(new window)

Cerci, I. H., P. Tatli, M. A. Azman and N. Birben. 2003. The effect of restricted feed on feed intake, egg production and feed conversion in pullets. Indian Vet. J. 80:1153-1157.

Chee, K. M., M. K. Chung, J. H. Choi and Y. K. Chung. 2005. Effects of dietary vitamins C and E on egg shell quality of broiler breeder hens exposed to heat stress. Asian-Aust. J. Anim. Sci. 18:545-551.

Cheng, T. K., C. N. Coon and M. L. Hamre. 1990. Effect of environmental stress on the ascorbic acid requirement of laying hens. Poult. Sci. 69:774-780.

Ciftci, M., O. N. Ertas and T. Guler. 2005. Effects of Vitamin E and Vitamin C dietary supplementation on egg production and egg quality of laying hens exposed to a chronic heat stres. Revue Med. Vet. 156:107-111.

Demir, E., O. Ozturkcan, M. Gorgulu, H. R. Kutlu and F. Okan. 1995. Sicak kosullarda yumurta tavugu rasyonlarina eklenen vitamin A ve C'nin yumurta ozelliklerine etkileri. J. Agric. Fac. C.U. 10:123-132.

Denli, M., S. Cankaya, S. Silici, F. Okan and A. N. Uluocak. 2005. Effect of dietary addition of Turkish propolis on the growth performance, carcass characteristics and serum variables of quail (Coturnix coturnix japonica). Asian-Aust. J. Anim. Sci. 18:848-854.

Dimov,V., N. Ivanovska, N. Manolova, V. Bankova, N. Nikolav and S. Popov. 1991. Immunomodulatoryn action of propolis. Influence on antiinfectious protection and macrophage function. Apidologie 22:155-162. crossref(new window)

Eisen, E. J., B. B.Bohren and H. E. Mckean. 1962. The haugh unit as a measure of egg albumen quality. Poult. Sci. 41:1461-1468.

El-Boushy, A. R., P. C. M. Simons and G. Wiertz. 1968. Structure and ultrastructure of the hen's egg shell as influenced by environmental temperature, humidity and vitamin C additions. Poult. Sci. 47:456-467.

Filizciler, M., I. H. Cerci and P. Tatli. 2002: Effects of night feeding on SPF (Specific Pathogen Free) white egg layers under heat stress (in Turkish). Turkish J. Vet. Anim. Sci. 26:439-446.

Foucher, D. 1982. Tesis. La propolis et son utilisation en Pharmacie. Th. Doct. Pharm. Clermont-Ferrand, France.

Freeman, B. M. 1987. The stres syndrome. World's Poult. Sci. J. 43:15-19. crossref(new window)

Ghisalberti, E. L. 1979. Propolis a Review. Bee World 60:59-84.

Giurgea, R., V. Toma, H. Popescu and C. Linicencu. 1981. Effects of standardized propolis extracts on certain blood constituents in chickens. Clujul- Medical. 54:151-154.

Haro, A., I. Lopez-Aliaga, F. Lisbona, M. Barrionuevo, M. J. M. Alferez and M. S. Campos. 2000. Beneficial effect of pollen and/or propolis on the metabolism of iron, calcium, phosphorus, and magnesium in rats with nutritional ferropenic anemia. J. Agric. Food Chem. 48:5715-5722. crossref(new window)

Havsteen, B. H. 2002. The biochemistry and medical significance of the flavonoids. Pharmacol. Therapeut. 96:67-202. crossref(new window)

Ipek, A., O. Canbolat and A. Karabulut. 2007. The effect of vitamin E and vitamin C on the performance of Japanase Quails (Coturnix Coturnics Japonica) reared under heat stress during growth and egg production period. Asian-Aust. J. Anim. Sci. 20:252-256.

Kimoto, N., H. Hirose Masao, M. Kawabe, T. Satoh, M. Hideki and T. Shira. 1999. Post-initiation effects of a super critical extract of propolis in a rat two-stage carcinogenesis model in female F344 rats. Cancer Letters. 147:221-227. crossref(new window)

Kumazawa, S., T. Hamaska and T. Nakayama. 2004. Antioxidant activity of propolis of various geographic origins. Food Chem. 84:329-339. crossref(new window)

Mc Kee, J. S. and P. C. Harrison. 1995. Effects of supplemental ascorbic acid on the performance of broiler chickens exposed to multiple concurrent stressors. Poult. Sci. 74:1772-1785.

Nagei, T., R. Inoue, H. Inoue and N. Suzuki. 2003. Prepartion and antioxidant properties of water extract of propolis. Food Chem. 80:29-33. crossref(new window)

Namgoong, S. Y., K. H. Son, H. W. Chang, S. S. Kang and H. P. Kim. 1994. Effects of naturally occurring flavonoids on mitogen-induced lymphocyte proliferation and mixed lymphocyte culture. Life Sci. 54:313-320. crossref(new window)

Njoku, P. C. and A. O. U. Nwazota. 1989. Effect of dietary inclusion of ascorbic acid and palm oil on the performance of laying hens in a hot tropical environment. Br. Poult. Sci. 30:831-840. crossref(new window)

NRC. 1994. Nutrient requirements of poultry National Academy Press.Washington, DC. Ninth Revised Edition, 19-34.

Onbasılar, E. E. and F. T. Aksoy. 2005. Stres parameters and immune response of layers under different cage flor and density conditions. Livest. Prod. Sci. 95:255-263. crossref(new window)

Petry, H. and W. Rapp. 1971. Zur Problematik der Chromoxidebestimmung in erdauungsversuchen. Z. Tierphysiol. Tierernahr. Futterm.-Kde. 27:181-189.

Prytzyk, E., A. P. Dantas, K. Salomao, A. S. Pereira, V. S. Bankova, S. L. D. Castro and F. R. A.Neto. 2003. Flavonoids and trypanocidal activity of bulgarian propolis. J. Ethnopharmacol. 88:189-193. crossref(new window)

Sahin, N. and K. Sahin. 2001. Optimal dietary concentrations of vitamin C and chromium picolinate for alleviating the effect of low ambient temperature (6.2$^{\circ}$C) on egg production, some egg characteristics, and nutrient digestibility in laying hens. Vet. Med.-Czech. 46:229-236.

Shalmany, S. K. and M. Shivazad. 2006. The effect of diet propolis supplementation on ross broiler chicks performance. Intl. J. Poult. Sci. 5:84-88. crossref(new window)

SPSS. 1999. Spss For Windows, 10.1 Spss Inc.

Taheri, H. R., H. R. Rahmani and J. Pourreza. 2005. Humoral immunity of broilers is affected by oil extracted propolis (OEP) in the diet. Intl. J. Poult. Sci. 4:414-417. crossref(new window)

Tatli Seven, P. 2006. Effects of selenium and vitamin C supplemented high energy diet on physical performance, nutrient retention and relative organ weights in cold stressed broilers (15$^{\circ}$C). International Symposium on Selenium In Health And Disease, October 12-13, Ankara, Turkey, p. 45.

Tatli Seven, P., I. Seven, M. Yılmaz and G. Simsek. 2008. The effects of Turkish propolis on growth and carcass characteristics in broilers under heat stress. Anim. Feed Sci. Technol. in press.

Tatli Seven, P., S. Yilmaz, I. Seven and B. Dalkilc. 2006. Effects of dietary supplementation of antioxidants (selenium and vitamin C), triiodothyronine (T$_3$) hormone and iodine on biochemical parameters and antioxidant enzyme activities in cold stressed broilers (15$^{\circ}$C). International Symposium on Selenium in Health and Disease, October 12-13, Ankara, Turkey, p. 44.

Terpstra, K. and N. De Hart. 1974. The estimation of urinary nitrogen and fecal nitrogen in poultry. Excreta. Z. Tierphysiol. Tierernahr. Futtem.-Kde. 32:306-320.

Toma, V., H. Popescu and C. Polinicencu. 1981. Effect of standardized propolis extracts on certain blood constituents of chicken. Clujul- Medical. 54:151-154.

Valle, M. L. 2000. Quantitative determination of antibacterian capacities of propolis. Apiacta. 35:152-161.

Wang, B. J., Y. H. Lien and Z. R. Yu. 2003. Supercritical fluid extractive fractionation-study of the antioxidant activities of propolis. Food Chem. 86:237-243. crossref(new window)

Webster, A. J. F. 1983. Nutrition and the thermal environment in nutritional physiology of farm animals (Ed. J. A. F. Rook and P. C. Thomas). New York, Longman, pp. 639-669.