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Effect of Genistein on Antioxidative Defence System and Membrane Fluidity in Chick Skeletal Muscle Cells
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 Title & Authors
Effect of Genistein on Antioxidative Defence System and Membrane Fluidity in Chick Skeletal Muscle Cells
Jiang, Z.Y.; Jiang, S.Q.; Lin, Y.C.; Ma, X.Y.; Xi, P.B.; Cao, T.; Wang, X.Q.;
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 Abstract
This study was conducted to investigate the protective effect of genistein on the antioxidative defence system and membrane fluidity in chick skeletal muscle cells after supplementation with 0, 20, 40, and genistein in treated cells for 24 h. Genistein supplementation recovered the decreased activity of total superoxide dismutase induced by , significantly increased glutathione peroxidase activity (p<0.05) and decreased malondialdehyde production (p<0.05). The treatment of 80 mol/L genistein in treated cells decreased the secretion of creatine kinase (p<0.05). Fluorescence polarization values and microviscosities observed with treated cells were significantly higher than those observed with no treated cells. The addition of genistein improved the increased fluorescence polarization value (p<0.05) caused by treatment. The microviscosity value was significantly decreased by adding genistein (p<0.05). In conclusion, genistein protected skeletal muscle cells from oxidative damage by improving antioxidative status and membrane fluidity.
 Keywords
Genistein;Lipid Peroxidation;Membrane Fluidity;Antioxidation;Chick Skeletal Muscle Cells;
 Language
English
 Cited by
1.
Cloning of Chicken Microsomal Glutathione S-transferase 1 Gene (MGST1) and Identification of Its Different Splice Variants,;;;;;;;;

아세아태평양축산학회지, 2009. vol.22. 2, pp.155-161 crossref(new window)
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