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Genetic Variation and Divergence among Swamp Buffalo, River Buffalo and Cattle: A Microsatellite Survey on Five Populations in China
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 Title & Authors
Genetic Variation and Divergence among Swamp Buffalo, River Buffalo and Cattle: A Microsatellite Survey on Five Populations in China
Zhang, Yi; Sun, Dongxiao; Yu, Ying; Zhang, Yuan;
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Domestic buffalo and cattle are two extremely important livestock species in worldwide agricultural production. In this paper, to investigate genetic diversity and divergence among swamp buffalo, river buffalo and cattle, 30 microsatellite markers were screened on 168 individuals sampled from five populations. Substantial differences were observed among the three groups of animals with respect to allele frequency distribution, allele size and polymorphism. The cattle sample (Mongolian) showed significantly higher genetic variability (0.674 of gene diversity, p<0.01), and the swamp and river buffalo samples displayed similar degree of genetic variation (0.536 in swamp and 0.546 in river, p = 0.92). Results of both phylogenetic tree and multivariate analysis could distinguish three groups of animals, suggesting their deep evolutionary divergence. Additionally, using genetic distance, we estimated a divergence time of 1.7 million years between swamp and river buffalo that strongly supported distinct genetic origins for the two buffalo types.
Buffalo;Cattle;Microsatellite;Genetic Diversity;Divergence;
 Cited by
De Novo Transcriptome Assembly of the Chinese Swamp Buffalo by RNA Sequencing and SSR Marker Discovery, PLOS ONE, 2016, 11, 1, e0147132  crossref(new windwow)
Barker, J. S. F., S. G. Tan, O. S. Selvarah and T. K. Mukherjee. 1997a. Genetic variation within and relationships among populations of Asian water buffalo (Bubalus bubalis). Anim. Genet. 28:1-13. crossref(new window)

Barker, J. S. F., S. S. Moore, D. J. S. Hetzel, D. Evans, S. G. Tan and K. Byrne. 1997b. Genetic diversity of Asian water buffalo (Bubalus bubalis): micro satellite variation and a comparison with protein-coding loci. Anim. Genet. 28: 103-115. crossref(new window)

Baumung, R., H. Simianer and I. Hoffmaun. 2004. Genetic diversity studies in farm animals - a survey. J. Anim. Breed. Genet. 121:361-373. crossref(new window)

Belkhir, K., P. Borsa, J. Goudet, L. Chikhi and F. Bonhomme. 1998. Genetix, logicial sous windows TM pour la genetique des populations. Montpellier. France. Available from

Botstein, D., R. L. White, M. Skolnick and R. W. Davis. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am. J. Human Genet. 32: 314-331.

Cockrill, W. R. 1974. The husbandry and health of the domestic buffalo. FAO, Rome.

Ellegren, H., S. Moore, N. Robinson, K. Byrne, W. Ward and B. C. Sheldon. 1997. Microsatellite evolution - a reciprocal study of repeat lengths at homologous loci in cattle and sheep. Mol. Biol. Evol. 14:854-860.

Estoup, A., L. Garnery, M. Solignac and J. M. Cornuet. 1995. Microsatellite variation in honey bee (Apis Mellifera L.) populations: hierarchical genetic structure and test of the infinite allele and stepwise mutation models. Genetics 140: 679-695.

Goldstein, D. B., A. R. Linares, L. L. Cavalli-Sforza and H. W. Feldman. 1995. An evaluation of genetic distance for use with micro satellite loci. Genetics 139:463-471.

Goudet, J. 2001. FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3). Available from

Hoffmann, I., P. A. Marsan, J. S. F. Barker, E. G. Cothran, O. Hanotte, J. A. Lenstra, D. Milan, S. Weigend and H. Simianer. 2004. New MoDAD marker sets to be used in diversity studies for the major farm animal species: recommendations of a joint ISAG/FAO working group. In: Proc. 29th International Conference on Animal Genetics. Tokyo, Japan. p. 107

Kumar, S., M. Nagarajan, J. S. Sandhu, N. Kumar, V. Behl and G. Nishanth. 2007. Mitochondrial DNA analyses of Indian water buffalo support a distinct genetic origin of river and swamp buffalo. Anim. Genet. 38:227-232. crossref(new window)

Lebart, L., A. Morineau and K. M. Warwick. 1984. Multivariate Descriptive Statistical Analysis. John Wiley & Sons, INC., New York

Lei, C. Z., W. Zhang, H. Chen, F. Lu, Q. L. Ge, R. Y. Liu, R. H. Dang, Y. Y. Yao, L. B. Yao, Z. F. Lu and Z. L. Zhao. 2007. Two maternal lineages revealed by mitochondrial DNA D-loop sequences in Chinese native water buffaloes (Bubalus bubalis). Asian-Aust. J. Anim. Sci. 20:471-476

Nei, M., F. Tajima and Y. Tateno. 1983. Accuracy of estimated phylogenetic trees from molecular data. II. Gene frequency data. J. Mol. Evol. 19:153-170. crossref(new window)

Ota, T. 1993. DISPAN: Genetic distance and phylogenetic analysis. Pennsylvania State University, University Park, PA

Ritz, L. R., M-L. Glowatzki-mullis, D. E. MacHugh and C. Gaillard. 2000. Phylogenetic analysis of the tribe Bovini using micro satellites. Anim. Genet. 31: 178-185. crossref(new window)

Sambrook, J., E. F. Fritsch and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York, NY.

Savage, D. E. and D. E. Russell. 1983. Mammalian paleo faunas of the world. Reading, MA

Scherf, B. D. 2000. World watch list for domestic animal diversity. 3nd edn. FAO, Rome

Sena, L., M. P. C. Schneider, B. Brenig, R. L. Honeycutt, J. E. Womack and L. C. Skow. 2003. Polymorphisms in MHC-DRA and -DRB alleles of water buffalo (Bubalus bubalis) reveal different features from cattle DR alleles. Anim. Genet. 34: 1-10. crossref(new window)

Sraphet, S., B. Moolmuang, A. Na-Chiangrnai, S. Panyim, D. R. Smith and K. Triwitayakorn. 2008. Use of cattle micro satellite markers to assess genetic diversity of thai swamp buffalo (Bubalus bubalis). Asian-Aust. J. Anim. Sci. 21:177-180.

Sukla, S., B. R. Yadav and T. K. Bhattacharya. 2006. Characterization of Indian riverine buffaloes by micro satellite markers. Asian-Aust. J. Anim. Sci. 19:1556-1560.

Tanaka, K., C. D. Solis, J. S. Masangkay, K. Maeda, Y. Kawamoto and Y. Namikawa. 1996. Phylogenetic relationship among all living species of the genus Bubalus based on DNA sequences of the cytochrome b gene. Biochem. Genet. 34:443-452. crossref(new window)

Vowles, E. J. and W. Amos. 2006. Quantifying ascertainment bias and species-specific length differences in human and chimpanzee micro satellites using genome sequences. Mol. Biol. Evol. 23:598-607. crossref(new window)

Zhang, Y., D. Sun, Y. Yu and Y. Zhang. 2006. A Y-liuked SNP in SRY gene differentiated Chinese indigenous swamp buffalo and introduced river buffalo. Asian-Aust. J. Anim. Sci. 19:1240-1244.

Zhang, Y., D. Sun, Y. Yu and Y. Zhang. 2007. Genetic diversity and differentiation of Chinese domestic buffalo based on 30 microsatellite markers. Anim. Genet. 38:569-575. crossref(new window)