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Milk Conjugated Linoleic Acid (CLA) Profile and Metabolic Responses of Dairy Cows Fed with High-temperature-micro-time (HTMT) Treated Diets Containing High Quantity Extruded Soybean (ESB)
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 Title & Authors
Milk Conjugated Linoleic Acid (CLA) Profile and Metabolic Responses of Dairy Cows Fed with High-temperature-micro-time (HTMT) Treated Diets Containing High Quantity Extruded Soybean (ESB)
Lee, H.G.; Hong, Z.S.; Wang, J.H.; Xu, C.X.; Jin, Y.C.; Kim, T.K.; Kim, Y.J.; Song, M.K.; Choi, Yun.-Jaei;
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A feeding trial was conducted to examine the effect of high-temperature-micro-time (HTMT) processing of diets containing extruded soybean (ESB) in high quantity on milk fat production, metabolic responses, and the formation of conjugated linoleic acid (CLA) and trans-vaccenic acid (TVA). Twenty-one multiparous Holstein cows in mid-lactation were blocked according to milk yield in the previous lactation. Cows within each block were randomly assigned to either normal concentrate or HTMT treated diets containing ESB (7.5% HTMT-ESB and 15% HTMT-ESB). It was hypothesized that the HTMT-ESB would affect the undegradable fatty acids in the rumen and, thus, would modify the fatty acid profile of milk fat. Both 7.5% and 15% HTMT-ESB did not affect milk yield, fat, protein, lactose and solid-not-fat (SNF), but the proportion of cis-9, trans-11 CLA in milk fat was significantly increased by these treatments. Content of TVA in milk fat was not affected by HTMT-ESB. The HTMT-ESB influenced the fatty acid profile in milk fat, but there was little difference between 7.5% and 15% of supplementation. HTMT-ESB feeding significantly decreased the concentration of plasma insulin and glucose, while plasma growth hormone (GH), triglyceride (TG), non-esterified fatty acid (NEFA) and HDLcholesterol were increased by 7.5% and 15% ESB-HTMT supplementation in comparison to the control group (p<0.05). However, no significant difference was observed in plasma LDL-cholesterol, insulin like growth factor (IGF)-1, T3, T4, and leptin concentrations among treatments (p>0.05). The present results showed that cis-9, trans-11 CLA production was increased by HTMT treatment of dietary ESB without reduction of milk fat, and the unchanged milk fat and yield was assumed to be associated with the constant level of thyroid hormones, leptin, and IGF-1.
HTMT;Milk Fat;CLA;Metabolic Response;Holstein Cow;
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AOAC. 1984. Official methods of analysis (14th ed.). Association of Official Analytical Chemists, Washington, DC

AOAC. 1990. Official methods of analysis. Association of Official Analytical Chemists, Washington, DC. Index No. 973.18

Amstalden, M., M. R. Garcia, S. W. Williams, R. L. Stanko, S. E. Nizielski, C. D. Morrison, D. H. Keisler and G. L. Williams. 2000. Leptin gene expression, circulating leptin, and luteinizing hormone pulsatility are acutely responsive to shortterm fasting in prebubertal heifers: relationships to circulating insulin and insulin-like growth factor I. Biol. Reprod. 63:127-133 crossref(new window)

Atwell, D. G., E. H. Jaster, K. J. Moore and R. L. Fernando. 1988. Evaluation of high oil and corn silage for lactating dairy cows. J. Dairy Sci. 71:2689-2698 crossref(new window)

Bass, J. J., J. M. Oldham, S. C. Hodgkinson, P. J. Fowke, H. Sauerwein, P. Molan, B. H. Breier and P. D. Gluckman. 1991. Influence of nutrition and bovine growth hormone (GH) on hepatic GH binding, insulin-like growth factor-I and growth of lambs. J. Endocrinol. 128:181-186 crossref(new window)

Bateman, H. G. and T. C. Jenkins. 1998. Influence of soybean oil in high fiber diets fed to nonlactating cows on ruminal unsaturated fatty acids and nutrient digestibility. J. Dairy Sci. 81:2451-2458 crossref(new window)

Bell, J. A. and J. J. Kennelly. 2000. Producing conjugated linoleic acid enriched milk through practical dairy nutrition. Conference abstract: 8th World Congress on Clinical Nutrition, Bankok, Thailaid. December. pp. 17-20

Belury, M. A. 1995. Conjugated dienoic linoleate. a polyunsaturated fatty acid with unique chemoprotective properties. Nutr. Rev. 53:83-89 crossref(new window)

Bessa, R. J., J. Santos-Silva, J. M. Ribeiro and A. V. Portugal. 2000. Reticulo-rumen biohydrogenation and the enrichment of ruminat edible products with linoleic acid conjugated isomers. Appl. Environ. Microbiol. 63:201-211 crossref(new window)

Block, E. L., D. Muller, L. C. Griel and D. L. Garwood. 1981. Brown midrib-3 corn silage and heated extruded soybeans for early lactating dairy cows. J. Dairy Sci. 64:1813-1819 crossref(new window)

Breier, B. H. and H. Sauerwein. 1995. Regulation of growth in ruminants by the somatotropic axis. In: Ruminant physiology: Digenstion, metabolism, growth and reproduction (Ed. W. Engelhardt, S. Leonhard-Marek, G. Breves and D. Giesecke). Stuttgart: Enke Verlag. pp. 102-123

Breier, B. H., P. D. Gluckman and J. J. Bass. 1988. The somatotrophic axis in young steers: influence of nutritional status and oestradiol-17β on hepatic high- and low-affinity somatotrophic biding sites. J. Endodrinol. 116:169-177 crossref(new window)

Chilliard, Y. 1993. Dietary fat and adipose tissue metabolism in ruminants, pigs, and rodents: a review. J. Dairy Sci. 76(12): 3897-3931 crossref(new window)

Chouinard, P. Y., V. Girard and G. J. Brisson. 1998. Fatty acid profile and physical properties of milk fat from cows fed calcium salts of fatty acids with varying unsaturation. J. Dairy Sci. 81:471-481 crossref(new window)

Chouinard, P. Y., L. Corneau, W. R. Butler, Y. Chilliard, J. K. Drackley and D. E. Bauman. 2001. Effect of dietary lipid source on conjugated linoleic acid concentrations in milk fat. J. Dairy Sci. 84:680-690 crossref(new window)

Daughaday, W. H., I. K. Mariz and S. L. Blethen. 1980. Inhibition of assess of bound somatomedin to membrane receptor and immunobinding sites: A comparision of radioreceptor and radioimmunoassay of somatomedin in native and acid-ethanol extraction serum. J. Clin. Endocrinol. Metab. 51:781-788 crossref(new window)

Dhiman, T. R., G. R. Anand, L. D. Satter and M. W. Pariza. 1999. Conjugated linoleic acid content of milk from cows fed different diets. J. Dairy Sci. 82:2146-2156 crossref(new window)

Duncan, D. B. 1955. Multiple ranges and multiple F test. Biometrics 11:1-42 crossref(new window)

Duckett, S. K., J. G. Andrae and F. N. Owens. 2002. Effect of high oil corn or added corn oil on ruminal biohydrogenation of fatty acids and conjugated linoleic acid formation in beef steers fed finishing diets. J. Anim. Sci. 80:3353-3360

Folch, J., M. Lee and G. H. Sloan-Stanley. 1957. A simple method for the isolation and purification of total lipids from animal tissue. J. Biol. Chem. 226:497-509

Gaynor, P. J., D. R. Waldo, A. V. Capuco, R. A. Erdman, L. W. Douglass and B. B. Teter. 1995. Milk fat depression, the glucogenic theory, and trans-C18:1 fatty acids. J. Dairy Sci. 78:2008-2015 crossref(new window)

Gaynor, P. J., R. A. Erdman, B. B. Teter, J. Sampugna, A. V. Capuco, D. R. Waldo and M. Hamosh. 1994. Milk fat yield and composition during abomasal infusion of cis or trans octadecenoates in Holstein cows. J. Dairy Sci. 77:157 crossref(new window)

Griinari, J. M., B. A. Corl, S. H. Lacy, P. Y. Chouinard, K. V. Nurmela and D. E. Bauman. 2000. Conjugated linoleic acid is synthesized endogenously in lactating dairy cows by Delta(9)- desaturase. J. Nutr. 130:2285-2291

Griinari, J. M., D. A. Dwyer, M. A. McGuire and D. E. Bauman. 1996. Partially hydrogenated fatty acids and milk fat depression. J. Dairy Sci. 79(Suppl.1):177(abstr)

Grum, D. E., J. K. Drackley, R. S. Younker, D. W. LaCount and J. J. Veenhuizen. 1996. Nutrition during the dry period and hepatic lipid metabolism of periparturient dairy cows. J. Dairy Sci. 79:1850-1864 crossref(new window)

Grummer, R. R. and D. J. Carroll. 1991. Effects of dietary fat on metabolic disorders and reproductive performance of dairy cattle. J. Anim. Sci. 69:3838-3852

Guillaume, B., D. E. Ottwerby, M. D. Stern, J. G. Linn and D. G. Johnson. 1991. Raw or extruded soybeans and rumenprotected methionine and lysine in alfalfa-based diets for dairy cows. J. Dairy Sci. 74:1912-1917 crossref(new window)

Harfoot, C. G. and G. P. Hazlewood. 1988. Lipid metabolism in the rumen. In: The rumen microbial ecosystem (Ed. P. N. Hobson). Elsevier Appl. Sci., London and NY. pp. 285-322

Houseknecht, K. L., J. P. Vanden Heuvel, S. Y. Moya-Camarena, C. P. Portocarrero, L. W. Peck, K. P. Nickel and M. A. Belury. 1998. Dietary conjugated linoleic acid normalizes impaired glucose tolerance in the Zucker diabetic fatty fa/fa rat. Biochem. Biophys. Res. Commun. 244:678-682 crossref(new window)

Ivan, M., P. S. Mir, K. M. Koenig, L. M. Rode, L. Neill, T. Entz and Z. Mir. 2001. Effects of dietary sunflower seed oil on rumen protozoa population and tissue concentration of conjugated linoleic acid in sheep. Small Rumin. Res. 41:215-227 crossref(new window)

Jenkins, T. C. 1993. Lipid metabolism in the rumen. J. Dairy Sci. 76(12):3851-3863 crossref(new window)

Kelly, M. L., J. R. Berry, D. A. Dwyer, J. M. Griinari, P. Y. Chouinard, M. E. V. Amburgh and D. E. Bauman. 1998. Dietary fatty acid sources affect conjugated linoleic acid concentrations in milk from lactating dairy cows. J. Nutr. 128: 881-885

Kelly, M. L., J. R. Berry, D. A. Dwyer, J. M. Griinari, P. Y. Chouinard, M. E. V. Amburgh and D. E. Bauman. 1998. Dietary fatty acid sources affect conjugated linoleic acid concentrations in milk from lactating dairy cows. J. Nutr. 128: 881-885

Kepler, C. R., K. P. Hirons, J. J. McNeill and S. B. Tove. 1966. Intermediates and products of the biohydrogenation of linoleic acid by Butyrivibrio fibrisolvens. J. Biol. Chem. 241:1350-1354 crossref(new window)

Kim, Y. J., R. H. Liu, D. R. Bond and J. B. Russell. 2000. Effect of linoleic acid concentration on conjugated linoleic acid production by Butyrivibrio fibrisolvens A38. Appl. Environ. Microbiol. 66:5226-5230 crossref(new window)

Kim, Y. J., K. W. Lee and H. J. Lee. 2003. Increase of conjugated Linoleic acid level in milk fat by bovine feeding regimen and ure fractionation. J. Microbiol. Biotechnol. 13:22-28

LaCount, D. W., J. K. Drackley, T. M. Cicela and J. H. Clark. 1995. High oil corn as silage or grain for dairy cows during an entire lactation. J. Dairy Sci. 78:1745-1754 crossref(new window)

Lee, H. G., R. A. Vega, L. T. Phung, N. Matsunaga, H. Kuwayama and H. Hidari. 2000. The effect of growth hormone releasing peptide-2 (KP102) administration on plasma insulin-like growth factor (IGF)-I and IGF-binding proteins in Holstein steers on different planes of nutrition. Domest. Anim. Endocrinol. 18(3):293-308 crossref(new window)

Lee, H. G., Y. J. Choi, S. R. Lee, H. Kuwayama, H. Hidari and S. K. You. 2005. Effects of dietary protein and growth hormonereleasing peptide (GHRP-2) on plasma IGF-1 and IGFBPs in Holstein steers. Domest. Anim. Endocrinol. 28:134-146 crossref(new window)

Lee, H. S., E. J. Kim, J. S. Cho, H. J. Cho, S. K. Lee, K. C. Jung and J. H. Y. Park. 1994. Conjugated linoleic acid decreases phosphorylated Akt levels in Mat-LyLu (MLL) rat prostate cancer cells. Food Sci. Biotechnol. 13:353-357

Lj$\varnothing$kjel, K., O. M. Harstad and A. Skrede. 2000. Effect of heat treatment of soybean meal and fish meal on amino acid digestibility in mink and dairy cows. Anim. Feed Sci. Technol. 84:83-95 crossref(new window)

Loor, J. J., A. Ferlay, A. Ollier, M. Doreau and Y. Chilliard. 2005. Relationship among trans and conjugated fatty acids and bovine milk fat yield due to dietary concentrate and linseed oil. J. Dairy Sci. 88:726-740 crossref(new window)

Loor, J. J., J. H. Herbein and C. E. Polan. 2002. Trans18:1 and 18:2 isomers in blood plasma and milk fat of grazing cows fed a grain supplement containing solvent-extracted or mechanically extracted soybean meal. J. Dairy Sci. 85:1197-1207 crossref(new window)

Mir, Z., L. A. Goonewardene, E. Okine, S. Jaegar and H. D. Scheer. 1999. Effect of feeding canola oil on constituents, conjugated linoleic acid (CLA) and fatty acid profiles in goats milk. Small Rumin. Res. 33:137-143 crossref(new window)

Murphy, J. J., J. F. Connolly and G. P. McNeill. 1995. Effects on cow performance and milk fat composition of feeding full fat soybeans and rapeseeds to dairy cows at pasture. Livest. Prod. Sci. 44:13-25 crossref(new window)

National Research Council. 1989. Nutrient requirement of dairy cattle. $6^{th}$ rev. ed. Natl. Acad. Sci. Washington, DC

Newbold, J. A., R. B. Heap, C. G. Prosser, R. H. Phipps, F. Adriaens and D. L. Hard. 1997. The effect of bovine somatotropin and diet on somatotropin binding sites in hepatic tissue of lactating dairy cows. J. Dairy Sci. 80:1085-1091 crossref(new window)

Nicolosi, R. J., E. J. Rogers, D. Kritchevski, J. A. Scimeca and P. J. Huth. 1997. Dietary conjugated linoleic acid reduces plasma lipoproteins and early aortic atherogenesis in hypercholesterolemic hamsters. Artery 22:266-277

Palmquist, D. L. 1988. The feeding value of fats. In: Feed Science: World Animal Science. Disciplinary Approach B4. Elsevier, Amsterdam. (Ed. E. R. Orskov). pp. 293-311

Park, Y., K. J. Albright, W. Liu, J. M. Storkson, M. E. Cook and M. W. Pariza. 1997. Effect of conjugated linoleic acid on body composition in mice. Lipids 32:853-858 crossref(new window)

Plath-Gabler, A., C. Gabler, F. Sinowatz, B. Berisha and D. Schams. 2001. The expression of the IGF family and GH receptor in the bovine mammary gland. J. Endocrinol. 168:39-48 crossref(new window)

Lepage, G. and C. C. Roy. 1986. Direct transesterification of all classes of lipids in a one-step reaction. J. Lipid Res. 27:114-120

Roh, S., I. J. Clarke, R. Xu, J. W. Goding, K. Loneragan and C. Chen. 1998. The in vitro effect of leptin on basal and growth hormone-releasing hormone-stimulated growth hormone secretion from the ovine pituitary gland. Neuroendocrinology 68:361-364 crossref(new window)

SAS. 1985. SAS User′s guide: Statistical Analysis Systems Institute, Inc., Cary, NC

Tollet, P., B. Enberg and A. Mode. 1990. Growth hormone (GH) regulation of cytochrome P-450IIC12, Insulin-like growth factor-I (IGF-I), and GH receptor messenger RNA expression in primary rat hepatocytes: a hormonal interplay with insulin, IGF-1 and thyroid hormone. Mol. Endocrinol. 4:1934-1942 crossref(new window)

Tsuchiya, T., Y. Nagao, A. Ozawa, M. Matsumoto, K. Sugahara, T. Kubo and H. Kato. 1998. Decrease of the obese gene expression in bovine subcutaneous adipose tissue by fasting. Biosci. Biotechnol. Biochem. 62:2068-2069 crossref(new window)

Van Dijk, H. J., G. D. O'Dell, P. R. Perry and L. W. Grimes. 1983. Extruded versus raw ground soybeans for dairy cow in early lactation. J. Dairy Sci. 66:2521-2533 crossref(new window)

Wang, J. H. and M. K. Song. 2001. Effect of sources and levels of carbohydrates on fermentation characteristics and hydrogenation of linoleic acid by rumen bacteria in vitro. Asian-Aust. J. Anim. Sci. 14:48-53

Wang, J. H. and M. K. Song. 2003. PH affects the in vitro formation of cis-9, trans-11CLA and trans11-octadecenoic acid by ruminal bacteria when incubated with oilseeds. Asian-Aust. J. Anim. Sci. 16:1743-1748

Wang, J. H., M. K. Song, Y. S. Son and M. B. Chang. 2002. Addition effect of seed-associated or free linseed oil on the formation of cis-9, trans-11 conjugated linoleic acid and octadecenoic acid by ruminal bacteria in vitro. Asian-Aust. J. Anim. Sci. 15:1115-1120

Wolf, M., S. H. Ingbar and A. C. Moses. 1989. Thyroid hormone and growth hormone interact to regulate insulin-like growth factor-1 messenger ribonucleic acid and circulation levels in the rat. Endocrinology 125:2905-2914 crossref(new window)