JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Effects of Sucrose and Trehalose on the Freezability of Markhoz Goat Spermatozoa
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effects of Sucrose and Trehalose on the Freezability of Markhoz Goat Spermatozoa
Khalili, B.; Farshad, Abbas; Zamiri, M.J.; Rashidi, A.; Fazeli, P.;
  PDF(new window)
 Abstract
The present study was conducted to observe the effect of increased osmolality of basic tris extender supplemented with trehalose and sucrose on post-thawing quality (motility, progressive motility, viability, the rate of acrosome abnormality, total abnormality and membrane integrity) of Markhoz goat spermatozoa. Fresh semen samples were evaluated for motility and sperm concentration. Only semen samples with motility more than 70% and sperm concentration higher than sperm/ml were used for cryopreservation. In Exp. 1, trehalose (50, 75 or 100 mM) and sucrose (40, 60 or 80 mM) were added to a basic tris diluent. Based on the results of experiment 1, the goal of Exp. 2 was to investigate the combinational effects of the highest and lowest concentrations ( or ) of trehalose and sucrose. As the control, semen was diluted and frozen in the tris diluent without trehalose or sucrose. The results in Exp. 1 showed that all evaluated spermatozoa characteristics improved significantly after freezing and thawing (p<0.05) and at the same time the increase of trehalose and sucrose concentrations in basic extenders was seen, with the best results obtained for extenders containing 70 and 100 mM trehalose and 80 mM sucrose. Comparing these results with those of control diluents, the effects of supplementation were significantly (p<0.05) better. In Exp. 2, the results showed no significant differences (p>0.05) between and extenders, but the results of were slightly better than obtained with diluents. Furthermore, the results of this experiment indicated that the sperm characteristics in the isotonic control extender were significantly (p<0.05) lower than examined extenders. In conclusion, the results of this study indicated that goat sperm can tolerate hypertonic trehalose and sucrose solutions better than isotonic control diluents in the freezing period. In particular, these positive effects have been shown for acrosome integrity, which is very important for the fertilization capacity of sperm. The data indicated that addition of trehalose plus sucrose to the freezing extender can be recommended for cryopreservation of goat spermatozoa, but more data is needed on pregnancy rate, acrosome reaction and IVF to ascertain the real effect.
 Keywords
Markhoz Goat;Cryopreservation;Semen;Trehalose;Sucrose;
 Language
English
 Cited by
 References
1.
Abdelhakeam, A. A., E. F. Graham, I. A.Vazquez and K. M. Chaloner. 1991. Studies on the absence of glycerol in unfrozen and frozen ram semen: Development of an extender for freezing- effect of osmotic pressure, egg yolk levels, type of sugars, and method of dilution. Cryobiology 28:43-49 crossref(new window)

2.
Aboagla, E. M. E. and T. Terada. 2003. Trehalose-enhanced fluidity of the goat sperm membrane and its protection during freezing. Biol. Reprod. 69:1245-1250 crossref(new window)

3.
Aisen, E. G., V. H. Medina and A. Venturino. 2002. Cryopreservation and post-thawed fertility of ram semen frozen in different trehalose concentrations. Theriogenology 57:1801-1808 crossref(new window)

4.
Amann, R. and B. Pickett. 1987. Principles of cryopreservation and a review of stallion spermatozoa. J. Equine Vet. Sci. 7:145-73 crossref(new window)

5.
Curry, M. R., J. D. Millar and P. F. Watson. 1994. Calculated optimal cooling rates for ram and human sperm cryopreservation fail to confirm with empirical observations. Biol. Reprod. 51:1014-1021 crossref(new window)

6.
De Leeuw, F. E., A. M. De Leeuw, J. H. Den Dass and V. Colenbrander. 1993. Effects of various cryoprotective agents and membrane stabilizing compounds on bull sperm membrane integrity after cooling and freezing. Cryobiology 30:32-44 crossref(new window)

7.
Evans, G. and W. M. S. Maxwell. 1987. Salamon's artificial insemination of sheep and goats. University Press, Sydney, NSW, Australia

8.
Farshad, A. and S. Akhondzadeh. 2008. Effects of sucrose and glycerol during the freezing step of cryopreservation on the viability of goat spermatozoa. Asian-Aust. J. Anim. Sci. 21:1721-1727

9.
Fernandez-Santos, M. R., F. Martinez-Pastor, V. Garcia-Macias, M. C. Esteso, A. J. Soler, P. de Paz, L. Anel and J. J. Garde. 2007. Extender osmolality and sugar supplementation exert a complex effect on the cryopreservation of Iberian red deer (Cervus elaphus hispanicus) epididymal spermatozoa. Theriogenology 67:738-753 crossref(new window)

10.
Hammerstedt, R. H., J. K. Graham and J. P. Nolan. 1990. Cryopreservation of mammalian sperm: what we ask them to survive. J. Androl. 11:73-88

11.
Honadel, T. E. and G. J. Killian. 1988. Cryopreservation of murine embryos with trehalose and glycerol. Cryobiology 25:331-337 crossref(new window)

12.
Isachenko, E. 2003. Vitrification of mammalian spermatozoa in the absence of cryoprotectans: from pst practical difficulties to present. Reprod. Biol. 6:191-200

13.
Liu, Z., R. H. Foote and C. C. Brockett. 1998. Survival of bull sperm frozen at different rates in media varying in osmolarity. Cryobiology 37:219-230 crossref(new window)

14.
Molinia, F. C., G. Evans, P. I. Casares and W. M. C. Maxwell. 1994a. Effect of monosaccharide and disaccharides in Trisbased diluents on motility, acrosome integrity and fertility of pellet frozen ram spermatozoa. Anim. Reprod. Sci. 36:113-122 crossref(new window)

15.
Molinia, F. C., G. Evans and W. M. C. Maxwell. 1994b. In vitro evaluation of zwitterion buffers in diluents for freezing ram spermatozoa. Reprod. Nutr. Dev. 34:491-500 crossref(new window)

16.
Purdy, P. H. 2006. A review on goat sperm cryopreservation. Small Rumin. Res. 63:215-225 crossref(new window)

17.
Matsuoka, T., H. Imai, H. Kohno and Y. Fukui. 2006. Effects of bovine serum albumin and trehalose in semen diluents for improvement of frozen-thawed ram spermatozoa. J. Reprod. Dev. 52:675-683 crossref(new window)

18.
Revell, S. G. and R. A. Mrode. 1994. An osmotic resistance test for bovine semen. Anim. Reprod. Sci. 36:77-86 crossref(new window)

19.
SAS. 1996. SAS/STAT Software: Changes and enhancements through release 6.12. SAS Institute Inc., Cary, NC, USA

20.
Sztein, J. M., J. Noble, S. Farley and L. E. Mobraaten. 2001. Comparison of permeating and non permeating cryoprotectants for mouse sperm cryopreservation. Cryobiology 42:28-39 crossref(new window)

21.
Storey, B. T., E. E. Noiles and K. A. Thompson. 1998. Comparison of glycerol, other polyols, trehalose, and raffinose to provide a defined cryoprotectant medium for mouse sperm cryopreservation. Cryobiology 37:46-58 crossref(new window)

22.
Watson, P. F. 2000. The causes of reduced fertility with cryopreserved semen. Anim. Reprod. Sci. 61:481-492 crossref(new window)

23.
Watson, P. F. and I. C. A. Martin. 1975. Effects of egg Yolk, glycerol and the freezing rate on the viability and acrosomal structures of frozen ram spermatozoa. Aust. J. Biol. Sci. 28:153-159

24.
Weitze, K. F. 1977. Untersuchungen zur Tiefgefriekonservierung von Kaninchensperma. Habil- Schr., Tieraerztl. Hochs. Hannover, Germany

25.
Woelders, H., A. Matthijs and B. Engel. 1997. Effects of trehalose and sucrose, osmolality of the freezing medium, and cooling rate on viability and intactness of bull sperm after freezing and thawing. Cryobiology 35:93-105 crossref(new window)

26.
Yildiz, C., A. Kaya, M. Aksoy and T. Tekeli. 2000. Influence of sugar supplementation of the extender on motility, viability and acrosomal integrity of dog spermatozoa during freezing. Theriogenology 54:579-585 crossref(new window)