Advanced SearchSearch Tips
Dietary L-arginine Supplementation Improves Intestinal Function in Weaned Pigs after an Escherichia coli Lipopolysaccharide Challenge
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Dietary L-arginine Supplementation Improves Intestinal Function in Weaned Pigs after an Escherichia coli Lipopolysaccharide Challenge
Liu, Yulan; Han, Jie; Huang, Jingjing; Wang, Xiaoqiu; Wang, Fenglai; Wang, Junjun;
  PDF(new window)
This study was conducted to determine whether L-arginine (Arg) supplementation could improve intestinal function in weaned pigs after an Escherichia coli lipopolysaccharide (LPS) challenge. Treatments included: i) non-challenged control (CONTR, pigs fed a control diet and injected with sterile saline); ii) LPS-challenged control (LPS, pigs fed the same control diet and challenged by injection with Escherichia coli LPS); iii) LPS+0.5% Arg (pigs fed a 0.5% Arg diet and challenged with LPS); and iv) LPS+1.0% Arg (pigs fed a 1.0% Arg diet and challenged with LPS). On d 16, pigs were administrated with LPS or sterile saline. D-xylose was orally administrated at 2 h following LPS challenge, and blood samples were collected at 3 h following LPS challenge. At 6 h post-challenge, pigs were sacrificed and intestinal mucosa samples were collected. Supplementation of Arg attenuated LPS-induced damage in gut digestive and barrier functions, as indicated by an increase in ileal lactase activity, and duodenal and ileal diamine oxidase activities (p<0.05). Arg administration also prevented the increase of jejunal malondialdehyde content and the decrease of ileal superoxide dismutase activity by LPS challenge (p<0.05). Furthermore, the jejunal nitric oxide level and inducible nitric oxide synthase activity were also improved after Arg supplementation (p<0.05). These results indicate that Arg supplementation has beneficial effects in alleviating the impairment of gut function induced by LPS challenge.
Arginine;Lipopolysaccharide;Weaned Pigs;Intestinal Function;
 Cited by
Effect of Glutamine, Glutamic Acid and Nucleotides on the Turnover of Carbon (δ13C) in Organs of Weaned Piglets,;;;;;;;;;

아세아태평양축산학회지, 2016. vol.29. 8, pp.1152-1158 crossref(new window)
LPS in weaned pigs, Innate Immunity, 2012, 18, 6, 804  crossref(new windwow)
LPS challenge, Innate Immunity, 2013, 19, 3, 242  crossref(new windwow)
Asparagine attenuates intestinal injury, improves energy status and inhibits AMP-activated protein kinase signalling pathways in weaned piglets challenged with Escherichia coli lipopolysaccharide, British Journal of Nutrition, 2015, 114, 04, 553  crossref(new windwow)
Effect of Glutamine, Glutamic Acid and Nucleotides on the Turnover of Carbon (δ13C) in Organs of Weaned Piglets, Asian-Australasian Journal of Animal Sciences, 2015, 29, 8, 1152  crossref(new windwow)
Fatty acids, inflammation and intestinal health in pigs, Journal of Animal Science and Biotechnology, 2015, 6, 1  crossref(new windwow)
Effects of l-carnitine and/or maize distillers dried grains with solubles in diets of gestating and lactating sows on the intestinal barrier functions of their offspring, British Journal of Nutrition, 2016, 116, 03, 459  crossref(new windwow)
Asparagine improves intestinal integrity, inhibits TLR4 and NOD signaling, and differently regulates p38 and ERK1/2 signaling in weanling piglets after LPS challenge, Innate Immunity, 2016, 22, 8, 577  crossref(new windwow)
N-Acetylcysteine improves intestinal function in lipopolysaccharides-challenged piglets through multiple signaling pathways, Amino Acids, 2017, 49, 12, 1915  crossref(new windwow)
Aspartate attenuates intestinal injury and inhibits TLR4 and NODs/NF-κB and p38 signaling in weaned pigs after LPS challenge, European Journal of Nutrition, 2017, 56, 4, 1433  crossref(new windwow)
Therapeutic Potential of Amino Acids in Inflammatory Bowel Disease, Nutrients, 2017, 9, 9, 920  crossref(new windwow)
AOAC. 1990. Official methods of analysis (15th Ed.). Association of Official Analytical Chemists, Washington, DC

Chander, V. and K. Chopra. 2005. Renal protective effect of molsidomine and L-arginine in ischemia-reperfusion induced injury in rats. J. Surg. Res. 128:132-139

Corl, B. A., J. Odle, X. M. Niu, A. J. Moeser, L. A. Gatlin, O. T. Phillips, A. T. Blikslager and J. M. Rhoads. 2008. Arginine activates intestinal $p70^{s6k}$ and protein synthesis in piglet rotavirus enteritis. J. Nutr. 138:24-29

Fang, Y. Z., S. Yang and G. Y. Wu. 2002. Free radicals, antioxidants, and nutrition. Nutrition 18:872-879 crossref(new window)

Fink, M. P., J. B. Antonsson, H. L. Wang and H. R. Rothschild. 1991. Increased intestinal permeability in endotoxic pigs. Mesenteric hypoperfusion as an etiologic factor. Arch. Surg. 126:211-218

Fotiadis, C., S. Adamis, E. P. Misiakos, M. Genetzakis, P. T. Antonakis, D. K. Tsekouras, V. G. Gorgoulis, G. C. Zografos, A. Papalois, M. Fotinou and D. Perrea. 2007. The prophylactic effect of L-arginine in acute ischaemic colitis in a rat model of ischaemia/reperfusion injury. Acta Chir. Belg. 107:192-200

Gao, C. J., W. Chai, L. X. Xu, G. H. Zhang, H. Zhang, L. C. Han and X. D. Sun. 2006. Protective effects of hyperoxygenated solution preconditioning on intestinal ischemia-reperfusion injury in rabbits. J. Surg. Res. 135:268-274 crossref(new window)

Gurbuz, A. T., J. Kunzelman and E. E. Ratzer. 1998. Supplemental dietary arginine accelerates intestinal mucosal regeneration and enhances bacterial clearance following radiation enteritis in rats. J. Surg. Res. 74:149-154 crossref(new window)

Hampson, D. J. and D. E. Kidder. 1986. Influence of creep feeding and weaning on brush border enzyme activities in the piglet small intestine. Res. Vet. Sci. 40:24-31

Han, J., Y. L. Liu, W. Fan, J. Chao, Y. Q. Hou, Y. L. Yin, H. L. Zhu, G. Q. Meng and Z. Q. Che. 2008. Dietary L-arginine supplementation alleviates immunosuppression induced by cyclophosphamide in weaned pigs. Amino Acids 10.1007/s00726-008-0184-9

Hou, Y. Q., Y. L. Liu, J. Hu and W. H. Shen. 2006. Effects of lactitol and tributyrin on growth performance, small intestinal morphology and enzyme activity in weaned pigs. Asian-Aust. J. Anim. Sci. 19:1470-1477

Kobayashi, H., T. Nonami, T. Kurokawa, Y. Takeuchi, A. Harada, A. Nakao and H. Takagi. 1995. Role of endogenous nitric oxide in ischemia-reperfusion injury in rat liver. J. Surg. Res. 59:772-779 crossref(new window)

Kohli, R., C. J. Meininger, T. E. Haynes, W. Yan, J. T. Self and G. Wu. 2004. Dietary L-arginine supplementation enhances endothelial nitric oxide synthesis in streptozotocin-induced diabetic rats. J. Nutr. 134:600-608

Kruzel, M. L., Y. Harari, C. Y. Chen and G. A. Castro. 2000. Lactoferrin protects gut mucosal integrity during endotoxemia induced by lipopolysaccharide in mice. Inflammation 24:33-44 crossref(new window)

Liu, G. P., W. P. Zhou, W. X. Zhu, G. M. Cheng, S. J. Jiang and S. G. He. 2003. The protective effect of L-arginine on the intestine barrier of rats with obstructive jaundice. J. Hepatobiliary Surg. 11:153-154 (Chinese)

Liu, K. X., T. Rinne, W. He, F. Wang and Z. Y. Xia. 2007. Propofol attenuates intestinal mucosa injury induced by intestinal ischemia-reperfusion in the rat. Can. J. Anesth. 54:366-374 crossref(new window)

Liu, Y., H. Liu and Q. Y. Kong. 2005. The roles of superoxide dismutase, nitric oxide synthase and nitric oxide in the antitumor mechanism of arsenic trioxide. Acta Acad. Med. Xuzhou. 25:8-12

Liu, Y. L., J. J. Huang, Y. Q. Hou, H. L. Zhu, S. J. Zhao, B. Y. Ding, Y. L. Yin, G. F. Yi, J. X. Shi and W. Fan. 2008a. Dietary arginine supplementation alleviates intestinal mucosal disruption induced by Escherichia coli lipopolysaccharide in weaned pigs. Br. J. Nutr. 100:552-560 crossref(new window)

Liu, Y. L., J. Lu, J. X. Shi, Y. Q. Hou, H. L. Zhu, S. J. Zhao, H. M. Liu, B. Y. Ding, Y. L. Yin and G. F. Yi. 2008b. Increased expression of the peroxisome proliferator-activated receptor $\gamma$ in the immune system of weaned pigs after Escherichia coli lipopolysaccharide challenge. Vet. Immunol. Immunopathol. 124:82-92 crossref(new window)

Lowry, O. H., N. J. Rosebrough, A. L. Farr and R. J. Randall. 1951. Protein measurement with folin phenol reagent. J. Biol. Chem. 193:265-275

Mansoori, B., H. Nodeh, M. Modirsanei, S. Rahbari and P. Aparnak. 2009. d-Xylose absorption test: A tool for the assessment of the effect of anticoccidials on the intestinal absorptive capacity of broilers during experimental coccidiosis. Anim. Feed Sci. Technol. 148:301-308 crossref(new window)

Mercer, D. W., G. S. Smith, J. M. Cross, D. H. Russell, L. Chang and J. Cacioppo. 1996. Effects of lipopolysaccharide on intestinal injury: potential role of nitric oxide and lipid peroxidation. J. Surg. Res. 63:185-192 crossref(new window)

Miguez, I., G. Marino, B. Rodríguez and C. Taboada. 2004. Effects of dietary L-arginine supplementation on serum lipids and intestinal enzyme activities in diabetic rats. J. Physiol. Biochem. 60:31-37 crossref(new window)

NRC. 1998. Nutrient requirements of swine (10th Ed.). National Academic Press, Washington, DC

Orlando, G. F., G. Wolf and M. Engelmann. 2008. Role of neuronal nitric oxide synthase in the regulation of the neuroendocrine stress response in rodents: insights from mutant mice. Amino Acids 35:17-27 crossref(new window)

Peng, X., H. Yan, Z. Y. You, P. Wang and S. L. Wang. 2004. Effects of enteral supplementation with glutamine granules on intestinal mucosal barrier function in severe burned patients. Burns 30:135-139 crossref(new window)

Rhoden, E. L., L. Pereira-Lima, C. R. Rhoden, M. L. Lucas, C. Teloken and A. Bello-Klein. 2001. Role of the L-arginine/nitric oxide pathway in renal ischaemia-reperfusion in rats. Eur. J. Surg. 167:224-228 crossref(new window)

Schleiffer, R. and F. Raul. 1996. Prophylactic administration of Larginine improves the intestinal barrier function after mesenteric ischaemia. Gut 39:194-198 crossref(new window)

Semrad, S. D. 2005. Malassimilation syndromes in large animals. In: Merck veterinary manual, ninth ed. (Ed. C. M. Kahn). Merck Co. Inc., Whitehouse Station, NJ, USA. pp. 301-306

Spanos, C. P., P. Papaconstantinou, P. Spanos, M. Karamouzis, G. Lekkas and C. Papaconstantinou. 2007. The effect of Larginine and aprotinin on intestinal ischemia-reperfusion injury. J. Gastrointest. Surg. 11:247-255 crossref(new window)

Sukhotnik, I., J. Mogilner, M. M. Krausz, M. Lurie, M. Hirsh, A. G. Coran and E. Shiloni. 2004. Oral arginine reduces gut mucosal injury caused by lipopolysaccharide endotoxemia in rat. J. Surg. Res. 122:256-262 crossref(new window)

Sukhotnik, I., H. Helou, J. Mogilner, M. Lurie, A. Bernsteyn, A. G. Coran and E. Shiloni. 2005. Oral arginine improves intestinal recovery following ischemia-reperfusion injury in rat. Pediatr. Surg. Int. 21:191-196 crossref(new window)

Verdonk, J. M. A. J., M. A. M. Spreeuwenberg, G. C. M. Bakker and M. W. A. Verstegen. 2001. Nutrient intake level affects histology and permeability of the small intestine in newly weaned piglets. In Digestive physiology of pigs (Ed. J. E. Lindberg and B. Ogle). pp. 332-334. Wallingford, UK: CABI Publishing

Wang, J. J., L. X. Chen, D. F. Li, Y. L. Yin, X. Q. Wang, P. Li, L. J. Dangott, W. X. Hu and G. Y. Wu. 2008a. Intrauterine growth restriction affects the proteomes of the small intestine, liver, and skeletal muscle in newborn pigs. J. Nutr. 138:60-66

Wang, J. J., L. X. Chen, P. Li, X. L. Li, H. J. Zhou, F. L. Wang, D. F. Li, Y. L. Yin and G. Y. Wu. 2008b. Gene expression is altered in piglet small intestine by weaning and dietary glutamine supplementation. J. Nutr. 138:1025-1032

Wang, W. W., S. Y. Qiao and D. F. Li. 2009. Amino acids and gut function. Amino Acids 37:105-110 crossref(new window)

Wu, G., F. W. Bazer, T. A. Cudd, W. S. Jobgen, S. W. Kim, A. Lassala, P. Li, J. H. Matis, C. J. Meininger and T. E. Spencer. 2007a. Pharmacokinetics and safety of arginine supplementation in animals. J. Nutr. 137:1673S-1680S crossref(new window)

Wu, G., F. W. Bazer, T. A. Davis, L. A. Jaeger, G. A. Johnson, S. W. Kim, D. A. Knabe, C. J. Meininger, T. E. Spencer and Y. L. Yin. 2007b. Important roles for the arginine family of amino acids in swine nutrition and production. Livest. Sci. 112:8-22 crossref(new window)

Wu, G., D. A. Knabe and S. W. Kim. 2004. Arginine nutrition in neonatal pigs. J. Nutr. 134:2783S-2390S

Wu, G., C. J. Meininger, D. A. Knabe, F. W. Bazer and J. M. Rhoads. 2000. Arginine nutrition in development, health and disease. Curr. Opin. Clin. Nutr. Metab. Care 3:59-66 crossref(new window)

Yao, K., Y. L. Yin, W. Y. Chu, Z. Q. Liu, D. Deng, T. J. Li, R. L. Huang, J.S. Zhang, B. Tan, W. C. Wang and G. Y. Wu. 2008. Dietary arginine supplementation increases mTOR signaling activity in skeletal muscle of neonatal pigs. J. Nutr. 138:867-872 crossref(new window)

Zhan, Z. F., D. Y. Ou, X. S. Piao, S. W. Kim, Y. H. Liu and J. J. Wang. 2008. Dietary arginine supplementation affects microvascular development in the small intestine of earlyweaned pigs. J. Nutr. 138:1304-1309

Zhang, C., Z. Y. Sheng, S. Hu, J. C. Gao, S. Yu and Y. Liu. 2002. The influence of apoptosis of mucosal epithelial cells on intestinal barrier integrity after scald in rats. Burns 28:731-737

Zhang, L. P., C. Y. Yang, Y. P. Wang, F. Cui and Y. Zhang. 2008. Protective effect of polydatin against ischemia/reperfusion injury in rat heart. Acta Physiologica Sinica 60:161-168

Zheng, C. T., T. Wang, Z. N. Lu, S. G. Zou, R. J. Xu and J. S. Zhang. 1999. EFfects of oral insulin and trypsinized formula milk on small intestinal growth and development in newborn pigs. Acta Veterinaria et Zootechnica Sinica. 30:405-413 (Chinese)