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Evaluation of Genetic Variation and Phylogenetic Relationship among North Indian Cattle Breeds
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 Title & Authors
Evaluation of Genetic Variation and Phylogenetic Relationship among North Indian Cattle Breeds
Sharma, Rekha; Pandey, A.K.; Singh, Y.; Prakash, B.; Mishra, B.P.; Kathiravan, P.; Singh, P.K.; Singh, G.;
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 Abstract
In the present study, genetic analyses of diversity and differentiation were performed on four breeds of Indian zebu cattle (Bos indicus). In total, 181 animals belonging to Ponwar, Kherigarh, Gangatiri and Kenkatha breeds were genotyped for 20 cattle specific microsatellite markers. Mean number of alleles observed per locus (MNA) varied between 5.75 (Kenkatha) to 6.05 (Kherigarh). The observed and expected heterozygosity for the breeds varied from 0.48 (Gangatiri) to 0.58 (Kherigarh) and 0.65 (Kenkatha) to 0.70 (Kherigarh), respectively. estimates of all the breeds indicated significant deficit of heterozygotes being 28.8%, 25.9%, 17.7% and 17.7% for Gangatiri, Ponwar, Kherigarh and Kenkatha, respectively. The estimates demonstrated that 10.6% was the average genetic differentiation among the breeds. Nei's genetic distance DA and Cavalli- Sforza and Edwards Chord distance () and the phylogenetic tree constructed from these reflected the close genetic relationship of Gangatiri and Kenkatha, whereas Ponwar appears to be more distant.
 Keywords
Cattle;Genetic Variation;India;Microsatellite;
 Language
English
 Cited by
 References
1.
Acharya, R. M. and P. N. Bhat. 1984. Livestock and poultry genetic resources of India. IVRI, Research Bulletin No. 1, Indian Veterinary Research Institute, Izatnagar, U.P. India

2.
Barker, J. S. F. 1999. Conservation of livestock breed diversity. AGRI. 25:33-43

3.
Barker, J. S. F., S. S. Moore, D. J. S. Hetzel, D. Evans, S. G. Tan and K. Byrne. 1997. Genetic diversity of Asian water buffalo (Bubalis bubalis): microsatellite variation and a comparison with protein coding loci. Anim. Genet. 28:103-115

4.
Bassam, B. J., G. Coetano-Anolles and P. M. Gresshoff. 1991. Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal. Biochem. 196:80-83 crossref(new window)

5.
Blott, S. C., J. L. Williams and C. S. Haley. 1998. Genetic variation within the Hereford breed of cattle. Anim. Genet. 29:202-211 crossref(new window)

6.
Bradley, D. G., D. G. Mac Hugh, R. T. Loftus, R. S. Sow, C. H. Hoste and E. P. Cunningham. 1994. Zebu-taurine variation in Y chromosome DNA: a sensitive assay for introgression in West African trypnotolerant cattle populations. Anim. Genet. 25:7-12

7.
Cavalli-Sforza, L. L. and A. W. F. Edwards. 1967. Phylogenetic analysis models and estimation procedure. Am. J. Human Genet. 19:233-257 crossref(new window)

8.
Crawford, A. M. and R. P. Cuthbertson. 1996. Mutations in sheep microsatellites. Genome Res. 6:876-879 crossref(new window)

9.
Dieringer, D. and C. Schlotterer. 2003. MICROSATELLITE ANANLYZER (MSA): A platform independent analysis tool for large microsatellite data sets. Molecular Ecology Notes 3:167-169 crossref(new window)

10.
Edwards, C., G. Dolf, C. Looft, R. Loftus and D. Bradley. 2000. Relationship between the endangered Pustertaler-Sprinzen and three related European cattle breeds as analyzed with 20 microsatellite loci. Anim. Genet. 31:329-332 crossref(new window)

11.
Felsenstein, J. 1993. PHYLIP: Phylogeny inference package. Version 3.5p, Department of Genetics, University of Washington, Seattle,WA

12.
Goldstein, D. V., A. Ruiz-Linares, L. L. Cavalla-Sforza and M. W. Heldman. 1995. Genetic absolute dating based on the origin of modern humans. Proc. Natl. Acad. Sci. USA. 92:6723-6727 crossref(new window)

13.
Goudet, J. 1995. FSTAT (version1.2): A computer programme to calculate F-statistics. J. Hered. 86:485-486

14.
Kantanen, J., I. Olsaker, L. E. Holm, S. Lian, J. Vilkki, K. Brusgaard, E. Eythorsdottir, B. Danell and S. Adalsteinsson. 2000. Genetic diversity and population structure of 20 North European cattle breeds. J. Hered. 91:446-457 crossref(new window)

15.
Livestock Census. 2003. Ministry of Statistics and Programme Implementation, Govt. of India

16.
MacHugh, D. E., R. T. Loftus, P. Cunningham and D. G. Bradley. 1998. Genetic structure of seven European cattle breeds assessed using 20 microsatellite markers. Anim. Genet. 29:333-340 crossref(new window)

17.
Maniatis, T., J. Sambrook and E. F. Fritish. 1982. Molecular cloning: A laboratory manual. 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York

18.
Mukesh, M., M. Sodhi, S. Bhatia and B. P. Mishra. 2004. Genetic diversity of Indian native cattle breeds as analyzed with 20 microsatellites. J. Anim. Breed. Genet. 121:416-424 crossref(new window)

19.
Naveen Kumar, S., C. S. Nagaraja, M. G. Govindaiah, S. M. Saravanan and K. Karthickeyan. 2006. Molecular characterization of hallikar breed of cattle using microsatellite markers. Asian-Aust. J. Anim. Sci. 19:622-626

20.
Nei, M. 1976. Mathematical models of speciation and genetic distance. In: Population genetics and ecology (Ed. S. Karlin and E. Nevo). pp. 723-766. Academic press, New York

21.
Nei, M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genet. 89:583-590 crossref(new window)

22.
Nei, M. 1983. Genetic polymorphism and the role of nutrition in evolution. In: Evolution of genes and proteins (Ed. M. Nei and R. Koehn). pp. 165-190. Sunderland, MA: Sinauer Associates

23.
Nei, M. 1987. Molecular evolutionary genetics. Columbia University Press, NewYork

24.
Page, R. D. 1996. TREEVIEW: An application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences, 12:357-358 crossref(new window)

25.
Pandey, A. K., R. Sharma, Y. Singh, B. Prakash and S. P. S. Ahlawat. 2006. Evaluation of genetic variability in kenkatha cattle by microsatellite markers. Asian-Aust. J. Anim. Sci. 19:1685-1690

26.
Pritchard, J. K., M. Stephens and P. Donnelly. 2000. Inference of population structure using multilocus genotype data. Genet. 155:945-959

27.
Schmid, M., N. Saitbekova, C. Gaillard and G. Dolf. 1999. Genetic diversity in Swiss cattle breeds. J. Anim. Breed. Genet. 116:1-8 crossref(new window)

28.
Shriver, M. D., L. Jin, E. Boerwinkle, R. Deka, R. E. Ferrell and R. Chakraborty. 1995. A novel measure of genetic distance for highly polymorphic tandem repeat loci. Genet. 12:914-920 crossref(new window)

29.
Sodhi, M., M. Mukesh, B. P. Mishra, K. R. Mitkari, B. Prakash and S. P. S. Ahlawat. 2005. Evaluation of Genetic differentiation in Bos indicus cattle breeds from Marathwada region of India using microsatellite polymorphism. Anim. Biotech. 16:127-137 crossref(new window)

30.
Takazaki, N. and M. Nei. 1996 Genetic distances and reconstruction of phylogenetic trees from microsatellite DNA. Genet. 144:389-399 crossref(new window)

31.
Weir, B. S. and C. C. Cockerham. 1984. Estimating F-statistics for the analysis of population structure. Evol. 38:1358-1370 crossref(new window)

32.
Yeh, Francis C., R-C Yang, B. J. Boyle Timothy, Z-H Ye and Judy X. Mao. 1999. POPGENE version 1.32, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Canada. (http://www.ualberta.ca/-fyeh/fyeh)