Advanced SearchSearch Tips
Lmbr1 Expression in Early Embryo Development Stages in White Leghorn and Chinese Silky
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Lmbr1 Expression in Early Embryo Development Stages in White Leghorn and Chinese Silky
Zhang, Ying; Xu, Weizhuo; Li, Ning;
  PDF(new window)
Lmbr1 is regarded as a key gene that controls the digital model formation in early developmental stages of the chicken. However, there are few reports of lmbr1 expression levels and tendencies in 4-toe and 5-toe chicken species. Therefore, the objective of this study was to compare the lmbr1 expression in White Leghorn (4-toe) and Chinese Silky (5-toe). Firstly, total RNA was extracted from 14 different embryonic development stages (HH3 to HH31) in White Leghorn and Chinese Silky. Secondly, dramatic gene expression changes of lmbr1 were monitored by RT-PCR, which indicated a general up-down-up tendency with subtle differences between these two species. Moreover, Q-PCR reactions were performed to quantitate the expression level of lmbr1 in the 14 selected developmental stages. These data demonstrated a first lmbr1 expression peak of 18.68 and 15.32, a lmbr1 expression trough of 6.61 and 1.80, and a second lmbr1 expression peak of 22.33 and 12.48 in White Leghorn and Chinese Silky, respectively. Finally, embryonic in situ hybridization analysis identified that lmbr1 expressed in the ectoderm in HH21, HH23 and HH24 developmental stages in both species.
Polydactyly;Lmbr1;Embryo Development;White Leghorn;Chinese Silky;
 Cited by
Clark, R. M., P. C. Marker and D. M. Kingsley. 2000. A novel candidate gene for mouse and human preaxial polydactyly with altered expression in limbs of Hemimelic extra-toes mutant mice. Genomics 67:19-27 crossref(new window)

Clark, R. M., P. C. Marker, E. Roessler, A. Dutra, J. C. Schimenti, M. Muenke and D. M. Kingsley. 2001. Reciprocal mouse and human limb phenotypes caused by gain- and loss-of-function mutations affecting Lmbr1. Genetics. 159:715-726 crossref(new window)

Hamburger, V. and H. L. Hamilton. 1951. A series of normal stages in the development of the chick embryo. J. Morphol. 88:49-92 crossref(new window)

Hill, R. E., S. J. Heaney and L. A. Lettice. 2003. Sonic hedgehog:restricted expression and limb dysmorphologies. J. Anat. 202:13-20 crossref(new window)

Horikoshi, T., N. Endo, M. Shibata, P. Heutink, R. E. Hill and S. Noji. 2003. Disruption of the C7orf2/Lmbr1 genic region is associated with preaxial polydactyly in humans and mice. J. Bone Miner. Metab. 21:1-4 crossref(new window)

Huang, Y. Q., N. Li, X. M. Deng, X. Qiu and W. Chen. 2004. Chicken polydactyly and its candidate gene. Chin. J. Anim. Sci. 40:39-41

Huang, Y. Q., X. M. Deng, Z. Q. Du, X. Qiu, X. Du, W. Chen, M. Morisson, S. Leroux, F. A. Ponce de Leon, Y. Da and N. Li. 2006. Single nucleotide polymorphisms in the chicken Lmbr1 gene are associated with chicken polydactyly. Gene. 374:10-18 crossref(new window)

Jang, H. Y., Y. S. Jung, H. T. Cheong, J. T. Kim, C. K. Park, H. S. Kong, H. K. Lee and B. K. Yang. 2008. Effects of cell status of bovine oviduct epithelial cell (BOEC) on the development of bovine IVM/IVF embryos and gene expression in the BOEC used or not used for the embryo culture. Asian-Aust. J. Anim. Sci. 21:980-987

Lettice, L. A., A. E. Hill, P. S. Devenney and R. E. Hill. 2008. Point mutations in a distant sonic hedgehog cis-regulator generate a variable regulatory output responsible for preaxial polydactyly. Hum. Mol. Genet. 17:978-985 crossref(new window)

Lettice, L. A., T. Horikoshi, S. J. Heaney, M. J. van Baren, H. C. van der Linde, G. J. Breedveld, M. Joosse, N. Akarsu, B. A. Oostra, N. Endo, M. Shibata, M. Suzuki, E. Takahashi, T. Shinka, Y. Nakahori, D. Ayusawa, K. Nakabayashi, S. W. Scherer, P. Heutink, R. E. Hill and S. Noji. 2002. Disruption of a long-range cis-acting regulator for Shh causes preaxial polydactyly. Proc. Natl. Acad. Sci. USA. 99:7548-7553 crossref(new window)

Loomis, C. A., R. A. Kimmel, C. X. Tong, J. Michaud and A. L. Joyner. 1998. Analysis of the genetic pathway leading to formation of ectopic apical ectodermal ridges in mouse Engrailed-1 mutant limbs. Development 125:1137-1148 crossref(new window)

Pitel, F., R. Berge, G. Coquerelle, R. P. Crooijmans, M. A. Groenen, A. Vignal and M. Tixier-Boichard. 2000. Mapping the naked neck (NA) and polydactyly (PO) mutants of the chicken with microsatellite molecular markers. Genet. Sel. Evol. 32:73-86 crossref(new window)

Maas, S. A. and J. F. Fallon. 2004. Isolation of the chicken Lmbr1 coding sequence and characterization of its role during chick limb development. Dev. Dyn. 229:520-528 crossref(new window)

Streit, A. and C. D. Stern. 2001. Combined whole-mount in situ hybridization and immunohistochemistry in avian embryos. Methods. 23:339-344 crossref(new window)

Tickle, C., D. Summerbell and L. Wolpert. 1975. Positional signaling and specification of digits in chick limb morphogenesis. Nature (Lond.). 254:199-202 crossref(new window)

Tickle, C. 1981. The number of polarizing region cells required to specify additional digits in the developing chick wing. Nature (Lond.). 289:295-298 crossref(new window)

Warren, D. C. 1944. Inheritance of Polydactylism in the Fowl. Genetics 29:217-231

Wu, W., R. F. Xu, X. Guo, C. H. Li and C. X. Wu. 2008. Characterization of embryonic feather follicle development in the Chinese indigenous Jilin White Goose. Asian-Aust. J. Anim. Sci. 21:346-352