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Net Portal Fluxes of Nitrogen Metabolites in Holstein Steers Fed Diets Containing Different Dietary Ratios of Whole-crop Corn Silage and Alfalfa Hay
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 Title & Authors
Net Portal Fluxes of Nitrogen Metabolites in Holstein Steers Fed Diets Containing Different Dietary Ratios of Whole-crop Corn Silage and Alfalfa Hay
EL-Sabagh, M.; Imoto, S.; Yukizane, K.; Yokotani, A.; Sugino, T.; Obitsu, T.; Taniguchi, K.;
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The objectives of the present study were to investigate the effects of different dietary ratios of whole-crop corn silage and alfalfa hay on nitrogen (N) digestion, duodenal flow and metabolism across the portal-drained viscera (PDV) of growing beef steers, and to elucidate their relationships. Four steers (2367 kg BW) fitted with duodenal cannulae and chronic indwelling catheters into the portal and mesenteric veins and abdominal aorta were used in a 44 Latin square design. Animals were fed (at 12-h intervals) the 4 diets consisting of whole-crop corn silage (C) and alfalfa hay (A) in 80:20 (C8A2), 60:40 (C6A4), 40:60 (C4A6) and 20:80 (C2A8) ratios of which dietary crude protein (CP) was 10.5, 12.0, 13.5 and 15.0% of dry matter (DM), respectively. Feeding level was restricted to 95% of ad libitum intake to measure N digestion, blood flow and net flux of N across the PDV. Digestibility of DM and neutral detergent fiber and digestible energy intake linearly increased as the ratio of alfalfa hay increased. The N intake, duodenal flow and intestinal disappearance increased linearly with increasing alfalfa hay. Arterial and portal concentrations of -amino N showed a quadratic response to increasing levels of alfalfa hay and were the highest in steers fed the C6A4 diet. The net PDV release of -amino N and ammonia N increased linearly with increasing alfalfa hay, but urea N uptake by PDV did not differ among diets. As a percentage of apparently digested N in the total gut, net PDV release of -amino N linearly decreased from 66 to 48% with increasing alfalfa hay. Conversely, net PDV recovery of -amino N to intestinal N disappearance varied with increasing alfalfa hay accounting for 49, 50, 58 and 61% on C8A2, C6A4, C4A6 and C2A8 diets, respectively. Net PDV uptake of urea N, relative to apparently digested N, linearly decreased from 81 to 25% as alfalfa hay increased from 20 to 80% of DM intake. Considering PDV uptake of urea N, microbial efficiency and conversion of total tract digested N to PDV -amino N net supply, a diet consisting of 80% whole-crop corn silage and 20% alfalfa hay (10.5% CP) was the best, while considering the quantities of intestinal N disappearance and -amino N absorption, a diet of 20% whole-crop corn silage and 80% alfalfa hay (15% CP) would be preferred. The proportion of -amino N recovered by PDV relative to the intestinal N disappearance may vary with energy intake level of mixed forage diets.
Growing Steer;Wholecrop Corn Silage;Alfalfa Hay;Digestion;Nitrogen Metabolism;Portal-drained Viscera;
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