JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Some Factors Affecting Freezing of Boar Semen in 5 ml Maxi-straws
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Some Factors Affecting Freezing of Boar Semen in 5 ml Maxi-straws
Dai, J.J.; Wu, C.F.; Zhang, Defu; Yin, F.Z.; Zhang, T.Y.; Liu, D.; Wu, H.L.; Li, L.L.; Yang, S.T.; Wang, L.;
  PDF(new window)
 Abstract
A series of experiments were conducted to determine the suitable freezing and thawing temperatures for the freezing of boar semen in 5 ml maxi-straws. The ultrastructure, in vitro fertilization (IVF) and artificial insemination (AI) of frozen-thawed semen were also be evaluated. The 5 cm freezing height gave the best results not only in post-thaw motility rate (54.00%), but also in normal acrosome morphology rate (NAR) (80.23%). There was no significant difference in the post-thaw motility between different thawing temperatures and corresponding thawing times (p>0.05); the group of and 25 s gave the highest motility rate (45.00%). As a whole, not only from the motility but also the NAR, thawing at was better than the other two treatments. In the freezing packages, 5 ml maxi-straw gave a little lower mobility (40%), viability rate (49.58%), plasma membrane integrity rate (53.91%) and NAR (52.65%) than the 0.25 ml straw, but there was no significant difference between the two straw volumes (p>0.05). The IVF capacity of frozen-thawed semen in this experiment was similar to fresh semen. From ultrastructure observation, the main damage to boar spermatozoa after freezing was seen in the acrosome, such as swelling and formation of vesicles. After AI in recipient Shanghai White sows, frozen-thawed semen from 5 ml maxi-straws and pellets produced 72.2% and 80% conception rate and 7.8 and 8 litter sizes, respectively, and there was no significant difference between the 5 ml maxi-straw and the pellet (p>0.05).
 Keywords
Boar;Semen;Freeze;5 ml Maxi-straw;
 Language
English
 Cited by
1.
Percoll 분리된 미니돼지 정액에서 LEY와 Triladyl을 이용한 동결융해후의 정자 성상 비교,이상희;유한준;이용승;정희태;양부근;김대영;박춘근;

Reproductive and Developmental Biology, 2010. vol.34. 1, pp.41-46
2.
Percoll 분리된 미니돼지 정액의 체외 수정 능력에 있어서 동결보존액의 영향,이상희;유한준;이용승;정희태;양부근;김대영;박춘근;

Reproductive and Developmental Biology, 2011. vol.35. 1, pp.85-91
 References
1.
Abeydeera, L. R and B. N. Day. 1997. Fertilization and subsequent development in vitro of pig oocytes inseminated in a modified Tris-buffered medium with frozen-thawed ejaculated spermatozoa. Biol. Reprod. 57:729-734 crossref(new window)

2.
Almlid, T., S. E. Stavne and L. A. Johnson. 1987. Fertility evaluation of the straw freezing technique for boar semen under practical I.A. conditions. Zuchthygiene (Beri). 22:193-202

3.
Almlid, T. and P. O. Hofmo. 1996. A brief review of frozen semen application under Norwegian AI service conditions. Reprod. Dom. Anim. 31:169-173 crossref(new window)

4.
Amann, R. P. 2005. Weaknesses in reports of "fertility" for horses and other species. Theriogenol. 63:698-715 crossref(new window)

5.
Berger, T., K. O. Turner, S. Meizel and J. L. Hedrick. 1985. Zona pellucida induced acrosome reaction in boar sperm. Biol. Reprod. 40:525-530 crossref(new window)

6.
Bwanga, C. O., M. M. de Braganca, S. Einarsson and H. Rodriguez-Martinez. 1990. Cryopreservation of boar semen in Mini- and Maxi-straws. J. Vet. Med. A, 37:651-658 crossref(new window)

7.
Bwanga, C. O. 1991. Cryopreservation of boar semen. Acta. Vet. Scand. 32:431-453

8.
Cabrita, E., V. Robles, R. Alvarez and M. P. Herráez. 2001. Cryopreservation of rainbow trout sperm in large volume straws: application to large scale fertilization. Aquaculture. 201(3-4):301-314 crossref(new window)

9.
Cheon, Y. M., H. K. Kim, C. B. Yang, Y. J. Yi and C. S. Park. 2002. Effect of season influencing semen characteristics, frozenthawed sperm viability and testosterone concentration in Duroc boars. Asian-Aust. J. Anim. Sci. 15(4):500-503

10.
Corcuera, B. D. 1996. Efecto de la presi´on osm´otica de los diluyentes de congelaci´on sobre la viabilidad del espermatozoide de verraco. Thesis UCM, School of Biological Sciences, Madrid

11.
Cordova-Izquierdo, A., J. H. Oliva, B. Lleo, C. Garcia-Artiga, B. D. Corcuera and J. F. Perez-Gutierrez. 2006. Effect of different thawing temperatures on the viability, in vitro fertilizing capacity and chromatin condensation of frozen boar semen packaged in 5 ml straws. Anim. Reprod. Sci. 92:145-154 crossref(new window)

12.
Cordova, A., J. F. Perez, B. Lleo, C. Garcia Artiga and S. Martin Rillo. 2001. In vitro fertilizing capacity of deep frozen boarsemen packaged in 0.5 and 5 ml straws. Reprod. Domest. Anim. 36:199-202

13.
Courtens, J. L. and M. Paquignon. 1985. Ultrastructure of fresh, frozen and frozen-thawed spermatozoa of the boar. In: Deep freezing of boar semen (Ed. L. A. Johnson and K. Larsson). Swedish University of Agricultural Sciences. Uppsala, pp. 61-87

14.
Eriksson, B. M. and H. Rodriguez-Martinez. 2000. Effect of freezing and thawing rates on the post-thaw viability of boar spermatozoa. Anim. Reprod. Sci. 63:205-220 crossref(new window)

15.
Fiser, P. S. 1990. Combined effect of glycerol concentration and cooling velocity on mobility and acrosomal integrity of boar spermatozoa frozen in 0.5 ml straws. Mol. Reprod. Dev. 25:123-129 crossref(new window)

16.
Fiser, P. S., R. W. Fairfull, C. Hansen, P. L. Panich, J. N. B. Shrestha and L. Underhill. 1993. The effect of warming velocity on motility and acrosome integrity of boar sperm as influenced by the rate of freezing and glycerol level. Mol. Reprod. Dev. 34:190-195 crossref(new window)

17.
Gil, M. A., L. R. Abeydeera, B. N. Day, J. M. Vazquez, J. Roca and E. Martinez. 2003. Effects of the volume of medium and number of oocytes during in vitro fertilizagtion on embryo development in pigs. Theriogenol. 60:767-776 crossref(new window)

18.
Hammitt, D. G., P. A. Martin and T. Callanan. 1989. Correlations between heterospermic fertility and assays of porcine seminal quality before and after cryopreservation. Theriogenol. 32:385-399 crossref(new window)

19.
Jiang, Z. L., Q. W. Li, W. Y. Li, J. H. Hu, H. W. Zhao and S. S. Zhang. 2007. Effect of low density lipoprotein on DNA integrity of freezing-thawing boar sperm by neutral comet assay. Anim. Reprod. Sci. 99:401-407 crossref(new window)

20.
Johnson, L. A., J. G. Aalbers, C. M. T. Willems and W. Sybema. 1981. Use of boar spermatozoa for artificial insemination. I. Fertilizing capacity of fresh and frozen spermatozoa in sows in 36 farms. J Anim Sci. 52:1130-1136 crossref(new window)

21.
Mazur, P. 1984. Freezing of live cells: mechanisms and implications. Am. J. Physiol. 247:125-142

22.
Nagai, T., K. Niwa and A. Iritani. 1984. Effect of sperm concentration during preincubation in a defined medium on fertilization in vitro of pig follicular oocytes. J. Reprod. Fertil. 70:271-275 crossref(new window)

23.
Nagai, T., T. Takahashi, H. Masuda, Y. Shioya, M. Kuwayama, M. Fukushima, S. Iwasaki and A. Hamada. 1988. In vitro fertilization of frozen boar spermatozoa. J. Reprod. Fertil. 84:585-591 crossref(new window)

24.
Pursel, V. G and L. A. Johnson. 1975. Freezing of boar spermatozoa: fertilizing capacity with concentrated semen and a new thawing procedure. J. Anim. Sci. 40:99-102

25.
Pursel, V. G and C. S. Park. 1985. Freezing and thawing procedures for boar spermatozoa (Ed. L. A. Johnson and K. Larsson). Deep freezing of boar semen. Uppsala, Sweden:147-166

26.
Pursel, V. G. and C. S. Park. 1987. Duration of thawing on post thaw acrosome morphology and motility of boar spermatozoa frozen in 5 ml maxi-straws. Theriogenol. 28:683-690 crossref(new window)

27.
Royere, D., S. Hamamah, J. C. Nicolle and J. Lansac. 1991. Chromatin alterations induced by freeze-thawing influence the fertilizing ability of human sperm. Int. J. Androl. 14:328-332 crossref(new window)

28.
Salamon, S., I. Wilmut and C. Polge. 1973. Deep freezing of boar semen. I. Effects of diluent composition, protective agents, and method of thawing on survival of spermatozoa. Aust. J. Biol. Sci. 26:219-230

29.
Sell$\acute{e}$s, J., J. Gadea, R. Romar, C. Matás and S. Ruiz. 2003. Analysis of in vitro fertilizing capacity to evaluate the freezing procedures of boar semen and to predict the subsequent fertility. Reprod. Dom. Anim. 38:66-72 crossref(new window)

30.
Soderquist, L., N. Madrid-Bury and H. Rodriguez-Martinez. 1997. Assessment of ram sperm membrane integrity following different thawing procedures. Theriogenol. 48:115-1125

31.
Thomas, C. A., D. L. Garner, M. J. DeJarnette and C. E. Marshall. 1998. Effect of cryopreservation on bovine sperm organelle function and viability as determined by flow cytometry. Biol. Reprod. 58:786-793 crossref(new window)

32.
Wang, W. H., K. Niwa and K. Okuda. 1991. In vitro penetration of pig oocytes matured in culture by frozen-thawed ejaculated spermatozoa. J. Reprod. Fertil. 93:491-496 crossref(new window)

33.
Watson, P. F. 1995. Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function. Reprod. Fertil. Dev. 7:871-889 crossref(new window)

34.
Weitze, K. F., D. Rath and G. Baron. 1987. Deep freezing of boar semen in plastic straws. Dstch.Tierartzl. Wschr. 94:485-488 crossref(new window)

35.
Westendorf, P., L. Richter and H. Treu. 1975. Zur Tiefgefrierung von ebersperma labor-und besamungsergebnisse mit dem hulsenberger pailletten-verfahren. Dstch. Tierartzl. Wschrr. 82:261-267

36.
Woelders, H. and D. Besten. 1993. Cryopreservation of boar semen with small between- boar variation of post- thaw sperm survival. Cryobiol. 30:645 crossref(new window)

37.
Woelders, H. and A. P. Malva. 1998. How important is the cooling rate in cryopreservation of (bull) semen, and what is its relation to thawing rate and glycerol concentration? Reprod. Dom. Anim. 33:299-305 crossref(new window)

38.
Wu, C. H., R. Rui, J. J. Dai, C. Y. Zhang, S. Q. Ju, B. Xie, X. Lu and X. F. Zheng. 2006. Dffects of cryopreservation on the developmental compentence, ultrastructure and cytoskeletal structure of porcine oocytes. Mol. Reprod. Dev. 73:1454-1462 crossref(new window)

39.
Yi, Y. J., H. J. Ko, S. H. Lee, C. B. Yang, D. S. Son, H. K. Kim and C. S. Park. 2004. In vitro fertilization and development of pig oocytes inseminated with boar sperm by different sperm washing media after thawing of the frozen straws. Asian-Aust. J. Anim. Sci. 17(2):164-167

40.
Zhou, J. B., K. Z. Yue, M. J. Luo, Z. L. Chang, H. Liang, Z. Y. Wang and J. H. Tan. 2004. Effect of extenders and temperatures on sperm viability and fertilizing capacity of Harbin white boar smen during long-term liqudi storage. Asian-Aust. J. Anim. Sci. 17(11):1501-1508