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Use of Nitrate-nitrogen as a Sole Dietary Nitrogen Source to Inhibit Ruminal Methanogenesis and to Improve Microbial Nitrogen Synthesis In vitro
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 Title & Authors
Use of Nitrate-nitrogen as a Sole Dietary Nitrogen Source to Inhibit Ruminal Methanogenesis and to Improve Microbial Nitrogen Synthesis In vitro
Guo, W.S.; Schaefer, D.M.; Guo, X.X.; Ren, L.P.; Meng, Qingxiang;
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An in vitro study was conducted to determine the effect of nitrate-nitrogen used as a sole dietary nitrogen source on ruminal fermentation characteristics and microbial nitrogen (MN) synthesis. Three treatment diets were formulated with different nitrogen sources to contain 13% CP and termed i) nitrate-N diet (NND), ii) urea-N diet (UND), used as negative control, and iii) tryptone-N diet (TND), used as positive control. The results of 24-h incubations showed that nitrate-N disappeared to background concentrations and was not detectable in microbial cells. The NND treatment decreased net production, but also decreased net production and increased net production. Total VFA concentration was lower (p<0.05) for NND than TND. Suppression of production and total VFA concentration may be linked to increased concentration of . The MN synthesis was greater (p<0.001) for NND than UND or TND (5.74 vs. 3.31 or 3.34 mg/40 ml, respectively). Nitrate addition diminished methane production as expected, but also increased MN synthesis.
Nitrate Nitrogen;Ruminal Methanogenesis;Microbial Nitrogen Synthesis;Gas Production;Rumen Fermentation;
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