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Effect of Different Rumen-degradable Carbohydrates on Rumen Fermentation, Nitrogen Metabolism and Lactation Performance of Holstein Dairy Cows
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 Title & Authors
Effect of Different Rumen-degradable Carbohydrates on Rumen Fermentation, Nitrogen Metabolism and Lactation Performance of Holstein Dairy Cows
Khezri, A.; Rezayazdi, K.; Mesgaran, M. Danesh; Moradi-Sharbabk, M.;
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 Abstract
Four multiparous lactating Holstein cows fitted with rumen cannulae were fed diets varying in the amount and source of rumen-degradable carbohydrates (starch vs. sucrose) to examine their effects on rumen fermentation, nitrogen metabolism and lactation performance. A Latin square with four diets and four periods of 28 days each was employed. Corn starch and sucrose were added to diets and corn starch was replaced with sucrose at 0 (0 S), 2.5 (2.5 S), 5.0 (5.0 S) 7.5% (7.5 S) of diet dry matter in a total mixed ration (TMR) containing 60% concentrate and 40% forage (DM basis). Replacing corn starch with sucrose did not affect (p>0.05) ruminal pH which averaged 6.41, but the ruminal pH for 7.5 S decreased more rapidly at 2 h after morning feeding compared with other treatments. Sucrose reduced () ruminal concentration (13.90 vs. 17.09 mg/dl) but did not affect peptide-N concentration. There was no dietary effect on total volatile fatty acids (110.53 mmol/L) or the acetate to propionate ratio (2.72). No differences (p>0.05) in molar proportion of most of the individual VFA were found among diets, except for the molar proportion of butyrate that was increased () with the inclusion of sucrose. Total branched chain volatile fatty acids tended to increase () for the control treatment (0 S) compared with the 7.5 S treatment. Dry matter intake, body weight changes and digestibility of DM, OM, CP, NDF and ADF were not affected by treatments. Sucrose inclusion in the total mixed ration did not affect milk yield, but increased milk fat and total solid percentage (). Sucrose tended () to increase milk protein percentage (3.28 vs. 3.05) and reduced () milk urea nitrogen concentration (12.75 vs. 15.48 mg/dl), suggesting a more efficient utilization of the rapidly available nitrogen components in the diet and hence improving nitrogen metabolism in the rumen.
 Keywords
Sucrose;Rumen Degradable Carbohydrates;Rumen Fermentation;Nitrogen Metabolism;Lactation Performance;
 Language
English
 Cited by
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Asian-Australasian Journal of Animal Sciences, 2010. vol.23. 7, pp.972-979 crossref(new window)
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Effects of Synchronization of Carbohydrate and Protein Supply on Ruminal Fermentation, Nitrogen Metabolism and Microbial Protein Synthesis in Holstein Steers,;;;;;;

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 References
1.
Allison, M. J. 1970. Nitrogen metabolism of ruminal microorganisms. In: Physiology of digestion and metabolism in the ruminant (Ed. A. T. Phillipson), Oriel Press, Newcastle upon Tyne, UK. p. 456

2.
AOAC. 1999. Official methods of analysis. 17th edn. Association of Official Analytical Chemists, Arlington, Virginia

3.
Ariza, P., A. Bach, M. D. Stern and M. B. Hall. 2001. Effects of carbohydrates from citrus pulp and hominy feed on microbial fermentation in continuous culture. J. Anim. Sci. 79:2713-2718

4.
Broderick, G. A., N. D. Luchini, W. J. Smith, S. Reynal, G. A. Varga and V. A. Ishler. 2000. Effect of replacing dietary starch with sucrose on milk production in lactating dairy cows. J. Dairy Sci. 83(Suppl. 1):248(Abstr.)

5.
Chen, G., J. B. Russell and C. J. Sniffen. 1987. A procedure for measuring peptides in rumen fluid and evidence that peptide uptake can be a rate-limiting step in ruminal protein degradation. J. Dairy Sci. 70:1211-1219 crossref(new window)

6.
Crook, W. M. and W. E. Simpson. 1971. Determination of ammonium in Kjeldahl digest of crops by an automated procedure. J. Sci. Food Agric. 22:9 crossref(new window)

7.
Hall, M. B. and C. Herejk. 2001. Differences in yields of microbial crude protein from in vitro fermentation of carbohydrates. J. Dairy Sci. 84:2486-2493 crossref(new window)

8.
Heldt, J. S., R. C. Cochran, C. P. Mathis, B. C. Woods, K. C. Olson, E. C. Titgemeyer, T. G. Nagaraja, E. S. Vanzant and D. E. Johnson. 1999. Effects of level and source of carbohydrate and level of degradable protein on intake and digestion of lowquality tallgrass-prairie hay by beef steers. J. Anim. Sci. 77:2846-2854

9.
Hristov, A. N. and J.-P. Jouany. 2005. Factors affecting the efficiency of nitrogen utilization in the rumen. In: Nitrogen and phosphorus nutrition of cattle and environment (Ed. A. N. Hristov and E. Pfeffer). CAB International, Wallingford, UK. pp. 117-166

10.
Huhtanen, P. and H. Khalili. 1991. Sucrose supplements in cattle given grass silage-based diet. 3. Rumen pool size and digestion kinetics. Anim. Feed Sci. Technol. 33:275-287 crossref(new window)

11.
Jones, D. F., W. H. Hoover and T. K. Miller-Webster. 1998. Effects of concentrations of peptides on microbial metabolism in continuous culture. J. Anim. Sci. 76:611-616

12.
Khalili, H. and P. Huhtanen. 1991. Sucrose supplements in cattle given grass silage-based diet. 2. Digestion of cell wall carbohydrates. Anim. Feed Sci. Technol. 33:263-273 crossref(new window)

13.
Lee, M. R. F., R. J. Merry, D. R. Davies, J. M. Moorby, M. O. Humphreys, M. K. Theodorou, J. C. MacRae and N. D. Scollan. 2003. Effect of increasing availability of watersoluble carbohydrates on in vitro rumen fermentation. Anim. Feed Sci. Technol. 104:59-70 crossref(new window)

14.
Leng, R. A. 1970. Formation and production of volatile fatty acids in the rumen. In: Physiology of digestion and metabolism in the ruminant (Ed. A T. Phillipson). Oriel Press, Newcastle upon Tyne, UK. pp. 406-421

15.
Mesgaran, M. D. and D. S. Parker. 1995. The effect of dietary protein and energy sources on ruminal accumulation of low molecular weight peptides in sheep. Anim. Sci. 60:535

16.
Miron, J., E. Yosef, D. Ben-Ghedalia, L. E. Chase, D. E. Bauman and R. Solomon. 2002. Digestibility by dairy cows of monosaccharide constituents in total mixed ration containing citrus pulp. J. Dairy Sci. 85:89-94 crossref(new window)

17.
Mould, F. L., E. R. $\phi$rskov and S. O. Mann. 1984. Associative effects of mixed feeds. I. Effect of type and level of supplementation and the influence on the rumen fluid pH on cellulolysis in vivo and dry matter digestion on various roughages. Anim. Feed Sci. Technol. 10:15-30 crossref(new window)

18.
National Research Council 2001. Nutrient Requirements of Dairy Cattle. 7th rev. edn. Natl. Acad. Sci., Washington, DC

19.
Nombekela, S. W. and M. R. Murphy. 1995. Sucrose supplementation and feed intake of dairy cows in early lactation. J. Dairy Sci. 78:880-885 crossref(new window)

20.
Ordway, R. S., V. A. Ishler and G. A. Varga. 2002. Effects of sucrose supplementation on dry matter intake, milk yield, and blood metabolites of periparturient Holstein dairy cows. J. Dairy Sci. 85:879-888 crossref(new window)

21.
Pate, F. 1983. Molasses in beef nutrition. Natl. Feed Ingredients Assoc., W. Des Moines, IA

22.
Robles, V., L. A. González, A. Ferret, X. Manteca and S. Calsamiglia. 2007. Effects of feeding frequency on intake, ruminal fermentation, and feeding behavior in heifers fed highconcentrate diets. J. Anim. Sci. 85:2538-2547 crossref(new window)

23.
Sannes, R. A., M. A. Messman and D. B. Vagnoni. 2002. Form of rumen-degradable carbohydrate and nitrogen on microbial protein synthesis and protein efficiency of dairy cows. J. Dairy Sci. 85:900-908 crossref(new window)

24.
SAS Institute Inc. 2000. SAS/STAT User's Guide: Version 8.1th edn. SAS Institute Inc., Cary, North Carolina

25.
Satter, L. D. and L. L. Slyter. 1974. Effect of ammonia concentration on rumen microbial protein production in vitro. Br. J. Nutr. 32:199-208 crossref(new window)

26.
Sniffen, C. J., J. D. O'Connor, P. J. Van Soest, D. G. Fox and J. B. Russell. 1992. A net carbohydrate and protein system for evaluating cattle diets. II. Carbohydrate and protein availability. J. Anim. Sci. 70:3562-3577

27.
Strobel, H. J. and J. B. Russell. 1986. Effect of pH and energy spilling on bacterial protein synthesis by carbohydrate-limited cultures of mixed rumen bacteria. J. Dairy Sci. 69:2941-2947 crossref(new window)

28.
Supelco, Inc. 1975. GC Separation of VFA C2-C5. Tech. Bull. 749D. Supelco, Inc., Bellefonte, PA

29.
Tamminga, S. 1992. Nutrition management of dairy cows as a contribution to pollution control. J. Dairy Sci. 75:345-357 crossref(new window)

30.
Vallimont, J. E., F. Bargo, T. W. Cassidy, N. D. Luchini, G. A. Broderick and G. A. Varga. 2004. Effects of replacing dietary starch with sucrose on ruminal fermentation and nitrogen metabolism in continuous culture. J. Dairy Sci. 87:4221-4229 crossref(new window)

31.
Van Keulen, J. and B. A. Young. 1977. Evaluation of acidinsoluble ash as a natural marker in ruminant digestibility studies. J. Anim. Sci. 44:282-287

32.
Van Soest, P. J., J. B. Robertson and B. A. Lewis. 1991. Methods for dietary fiber, neutral fiber and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3588-3597

33.
Van Soest, P. J. 1994. Nutritional ecology of the ruminant. 2nd Ed. Cornell Univ. Press, Ithaca, NY

34.
Varga, G. A. 2003. Soluble carbohydrates for lactating dairy cows. In: Proceedings of tri state dairy nutrtion. Conferance., Fort Wayne, IN. p. 59

35.
Wallace, R. J. 1996. Ruminal microbial metabolism of peptides and amino acids. J. Nutr. 126:1326S-1334S crossref(new window)