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Responses of Dairy Cows to Supplemental Highly Digestible Rumen Undegradable Protein and Rumen-protected Forms of Methionine
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Responses of Dairy Cows to Supplemental Highly Digestible Rumen Undegradable Protein and Rumen-protected Forms of Methionine
Sun, T.; Yu, X.; Li, S.L.; Dong, Y.X.; Zhang, H.T.;
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Metabolizable protein (MP) supply and amino acid balance in the intestine were manipulated through selection of highly digestible rumen-undegradable protein (RUP) sources and protected methionine (Met) supplementation. Four ruminallycannulated, multiparous Holstein cows averaging 19313 days in milk were used in a 44 Latin square design to assess N utilization and milk production responses to changes in RUP level, post-ruminal RUP digestibility and protected Met supplementation. Treatments were A) 14.0% crude protein (CP), 8.0% rumen degradable protein (RDP) and 6.0% RUP of low intestinal digestibility (HiRUP-LoDRUP); B) 14.1% CP, 8.1% RDP and 6.0% RUP of high intestinal digestibility (HiRUP-HiDRUP); C) 13.1% CP, 7.9% RDP and 5.2% RUP of high intestinal digestibility (LoRUP-HiDRUP), and D) 13.1% CP, 7.9% RDP and 5.2% RUP of high intestinal digestibility plus rumen escape sources of Met (LoRUP-HiDRUP+Met). Experimental diets were formulated to have similar concentrations of RDP, net energy of lactation (), neutral detergent fiber (NDF), acid detergent fiber (ADF), calcium, phosphorus and ether extract using the NRC model (2001). Results showed that dry matter intake (DMI), production of milk fat and protein were similar among treatments. Milk production was similar for diet HiRUP-LoDRUP, HiRUP-HiDRUP and LoRUP-HiDRUP+Met, and significantly higher than diet LoRUP-HiDRUP. Milk fat and protein percentage were higher for cows receiving HiDRUP treatments, with the greatest increases in the diet LoRUP-HiDRUP+Met. There was no significant change in ruminal pH, and volatile fatty acid (VFA) concentration among all treatments. Apparent digestibility of dry matter (DM), CP, NDF and ADF and estimated bacterial CP synthesis were similar for all treatments. Nitrogen intakes, blood and milk urea-N concentrations were significantly higher for cows receiving HiRUP diets. Urine volume and total urinary N excretion were significantly lowered by LoRUP diets. Lowering dietary RUP level while supplementing the highly digestible RUP source with rumen escape sources of Met resulted in similar milk production, maximal milk fat and protein concentration and maximum N efficiency, indicating that post-ruminal digestibility of RUP and amino acid balance in the small intestine can be more important than total RUP supplementation.
Rumen Undegradable Protein;Methionine;Nitrogen Efficiency;
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AOAC. 1990. Official methods of analysis. 15th Edition. Association of Official Analytical Chemists International, Arlington, VA

Armentano, L. E., S. J. Bertics and G. A. Ducharme. 1997. Response of lactating cows to methionine or methionine plus lysine added to high protein diets based on alfalfa and heated soybeans. J. Dairy Sci. 80:1194-1199 crossref(new window)

Belal, S. O., Y. A. Abdullah, S. A. Mofleh, T. K. Rami, H. T. Hosam and I. Q. Rasha. 2008. Effect of methionine supplementation on performance and carcass characteristics of Awassi ram lambs fed finishing diets. Asian-Aust. J. Anim. Sci. 21:831-837

Broderick, G. A. and J. H. Kang. 1980. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. J. Dairy Sci. 63:64-75 crossref(new window)

Broderick, G. A. and M. K. Clayton. 1997. A statistical evaluation of animal and nutritional factors influencing concentrations of milk urea nitrogen. J. Dairy Sci. 80: 2964-2971 crossref(new window)

Calsamiglia, S. and M. D. Stern. 1995. A three-step in vitro procedure for estimating intestinal digestion of protein in ruminants. J. Anim. Sci. 73:1459-1465 crossref(new window)

Erasmus, L. J., P. M. Botja and C. W. Cruywagen. 1993. Amino acid profile and intestinal digestibility in dairy cows of rumenundegradable protein from various feedstuffs. J. Dairy Sci. 77: 541-551 crossref(new window)

Erwin, E. S., G. J. Marco and E. M. Emery. 1961. Volatile fatty acid analysis of blood and rumen fluid by gas chromatography. J. Dairy Sci. 44:1768-177 crossref(new window)

Flis, S. A. and M. A. Wattiaux. 2005. Effects of parity and supply of rumen-degraded and undegraded protein on production and nitrogen balance in holsteins. J. Dairy Sci. 88:2096-2106 crossref(new window)

Huber, J. T., R. S. Emery, W. G. Bergen, J. S. Liesman, L. Kung, K. J. King, R. W. Gardner and M. Checketts. 1984. Influences of methionine hydroxy analog on milk and milk fat production, blood serum lipids, and plasma amino acids. J. Dairy Sci. 67:2525-2531 crossref(new window)

Kalscheur, K. F., V. I. Baldwin, B. P. Glenn and R. A. Kohn. 2006. Milk production of dairy cows fed differing concentrations of rumen-degraded protein. J. Dairy Sci. 89:249-259 crossref(new window)

Koenig, K. M., L. M. Rode, C. D. Knight and M. Vazquez-Anon. 2002. Rumen degradation and availability of various amounts of liquid methionine hydroxy analog in lactating dairy cows. J. Dairy Sci. 85:930-938 crossref(new window)

Lundquist, R. G., J. G. Linn and D. E. Otterby. 1983. Influence of dietary energy and protein on yield and composition of milk from cows fed methionine hydroxy analog. J. Dairy Sci. 66:475-491 crossref(new window)

McCarthy, R. D., G. A. Porter and L. C. Griel. 1968. Bovine ketosis and depressed fat test in milk: a problem of methionine metabolism and serum lipoprotein aberration. J. Dairy Sci. 51: 459-466 crossref(new window)

National Research Council 2001. Nutrient requirements of dairy cattle. 7th revised ed. Natl. Acad. Sci., Washington, DC

Noftsger, S. and N. R. St-Pierre. 2003. Supplementation of Methionine and selection of highly digestible rumen undegradable protein to improve nitrogen efficiency for milk production. J. Dairy Sci. 86:958-969 crossref(new window)

Olmos Colmenero, J. J. and G. A. Broderick. 2006. Effect of dietary crude protein concentration on milk production and nitrogen utilization in lactating dairy cows. J. Dairy Sci. 89: 1704-1712 crossref(new window)

Reynal, S. M., G. A. Broderick, S. Ahvenjarvi and P. Huhtanen. 2003. Effect of feeding protein supplements of differing degradability on omasal flow of microbial and undegraded protein. J. Dairy Sci. 86:1292-1305 crossref(new window)

Rulquin, H. and L. Delaby. 1997. Effects of the energy balance of dairy cows on lactational responses to rumen-protected methionine. J. Dairy Sci. 80:2513-2522 crossref(new window)

Santos, F. A. P., J. E. P. Santos, C. B. Theurer and J. T. Huber. 1998. Effects of rumen-undegradable protein on dairy cow performance: A 12-year literature review. J. Dairy Sci. 81:3182-3213 crossref(new window)

SAS Institute Inc. 1999. SAS/STAT user's guide: Version 8. 4th edn. SAS Institute Inc., Cary, North Carolina

Schwab, C. G. 1998. Methionine analogs for dairy cows: A subject revisited. 1998 California Animal Nutr. Conf., Fresno, CA

St-Pierre, N. and C. S. Thraen. 1999. Animal grouping strategies, sources of variation, and economic factors affecting nutrient balance on dairy farms. J. Anim. Sci. 77(Supp. 2):72-83

Vagnoni, D. B., G. A. Broderick, M. K. Clayton and R. D. Hatfield. 1997. Excretion of purine derivatives by Holstein cows abomasally infused with incremental amounts of purines. J. Dairy Sci. 80:1695-1702 crossref(new window)

Van Soest, P. J., J. B. Robertson and B. A. Lewis. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74: 3583-3597

Wattiaux, M. A. and K. L. Karg. 2004. Protein level for alfalfa and corn silage based diets: I. Lactational response and milk urea nitrogen. J. Dairy Sci. 87:3480-3491 crossref(new window)