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Low Lysine Treatment Increases Adipogenic Potential of Bovine Intramuscular Preadipocytes
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 Title & Authors
Low Lysine Treatment Increases Adipogenic Potential of Bovine Intramuscular Preadipocytes
Beloor, Jagadish; Kang, Hye Kyeong; Yun, Cheol-Heui; Kim, Sang Hoon; Moon, Yang Soo;
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The molecular mechanism of adipocyte differentiation has been well documented. However, the effect of specific nutrients such as lysine on adipocyte differentiation is poorly understood especially in ruminant animals. Therefore, the aim of the present study was to elucidate the influence of lysine on adipocyte differentiation and adipogenic genes in cultured bovine preadipocytes. The preadipocytes were treated with different concentrations of lysine (40, 160, 320 mg/L) or troglitazone (10 ) for 2 days and then subsequently cultured in differentiation medium until day 6. Expression levels of were significantly higher (p<0.001) in 40 and 160 mg/L lysine-treated cells compared to 320 mg/L treatment. Though there was an increasing trend in expression levels with the decreasing lysine concentration, the results were not significant. The preadipocyte factor (pref-1), expression significantly (p<0.001) reduced with decreasing lysine concentration. The Oil red O staining results were better in 40 mg/L treated cells compared to 160 and 320 mg/L lysine treated cells. Our overall results indicate that insufficient supply of lysine increases the adipogenic potential in bovine intramuscular preadipocytes.
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아세아태평양축산학회지, 2009. vol.22. 9, pp.1320-1327 crossref(new window)
Adeola, O. and L. G. Young. 1989. Dietary protein-induced changes in porcine muscle respiration, protein synthesis and adipose tissue metabolism. J. Anim. Sci. 67:664-673 crossref(new window)

Aso, H., I. Abe, K. Nakajima, T. Ozutsumi, Yamaguchi and Y. Takamori. 1995. A preadipocyte clonal line from bovine intramuscular adipose tissue, Biochem. Biophys. Res. Commun. 213:369-375 crossref(new window)

Bluher, M. 2005. Transgenic adipocyte differentiation, animal models for the study of adipose tissue biology. Best Pract. Res. Clin. Endocrinol. Metab. 19:605-623 crossref(new window)

Butterwith, S. C. 1994. Molecular events in adipocyte development. Pharmacol. Ther. 61:399-411 crossref(new window)

Candek-Potokar, M., L. Lefaucheur, B. Zlender and M. Bonneau. 1998. Effect of age and/or weight at slaughter on longissimus dorsi muscle: biochemical traits and sensory quality in pigs. Meat Sci. 48:287-300 crossref(new window)

Castell, A.G., R. L. Cliplef, L. M. Poste-Flynn and G. Butler. 1994. Performance, carcass and pork characteristics of castrates and gilts self-fed diets differing in protein content and lysine: Energy ratio. Canadian J. Anim. Sci. 74:519-528 crossref(new window)

Fernyhough, M. E., D. L. Helterline, J. L. Vierck, G. J. Hausman, R. A. Hill and M. V. Dodson. 2005. Dedifferentiation of mature adipocytes to form adipofibroblasts: more than just a possibility. Adipocytes 1:17-24 crossref(new window)

Fernyhough, M. E., E. Okine, G. J. Hausman, J. L. Vierck and M. V. Dodson. 2007. Invited review: PPAR-gamma and GLUT-4 expression as differentiation markers for preadipocyte conversion to become an adipocyte. Domest. Anim. Endocrinol. 33:367-378 crossref(new window)

Gondret, F. and B. Lebret. 2002. Feeding intensity and dietary protein level affect adipocyte cellularity and lipogenic capacity of muscle homogenates in growing pigs, without modification of the expression of sterol regulatory element binding protein. J. Anim. Sci. 80:3184-3193

Hausman, D. B. and M. DiGirolamo. 2001. The biology of white adipocyte proliferation. Obes. Rev. 2:239-254 crossref(new window)

Hong, Y. H., Y. Nishimura, D. Hishikawa, H. Tsuzuki, H. Miyahara, C. Gotoh, K. C. Choi, D. D. Feng, C. Chen, H. G. Lee, K. Katoh, S. G. Roh and S. Sasaki. 2005. Acetate and propionate short chain fatty acids stimulate adipogenesis via GPCR43. Endocrinol. 146:5092-5099 crossref(new window)

Jeong, H. Y., M. S. Lee, H. Kim, Y. H. Park, D. Yoon, S. J. Moon, A. Hosoda, H. D. Kim, S. Saeki and M. J. Kang. 2008. Cloning, expression, and regulation of bovine cellular retinoic acid-binding protein-2 (CRABP-2) during adipogenesis. Asian-Aust. J. Anim. Sci. 21:1551-1558

Katsumata, M., M. Matsumoto, S. Kobayashi and Y. Kaji. 2007. Reduced dietary lysine enhances proportion of oxidative fibers in porcine skeletal muscle. Anim. Sci. J. 79:347-353

Kawada, T., Y. Kamei and E. Sugimoto. 1996. The possibility of active form of vitamins A and D as suppressors on adipocyte development via ligand-dependent transcriptional regulators. Int. J. Obes. Relat. Metab. Disord. 20:52-57

Kerr, B. J., F. K. McKeith and R. A. Easter. 1995. Effect on performance and carcass characteristics of nursery to finisher pigs fed reduced crude protein, amino acid-supplemented diets, J. Anim. Sci. 73:433-440

Kokta, T. A., M. V. Dodson, A. Gertler and R. A. Hill. 2004. Intercellular signalling between adipose tissue and muscle tissue. Domest. Anim. Endocrinol. 27:303-331 crossref(new window)

Lee, S. C., H. J. Lee, D. W. Kim and K. I. Han. 2000. Effect of hormones on proliferation of stromal vascular cells from Hanwoo cattle adipose tissue. Asian-Aust. J. Anim. Sci. 13:161-166

Ntambi, M. and Y. C. Kim. 2000. Adipocyte differentiation and gene expression, J. Nut. 130:3122-3126

Ritchie, H. D., S. R. Rust, R. A. Merkel and W. G. Bergen. 1993. Getting rid of excess fat is not an easy task. Feedstuffs 13

SAS. 1999. SAS/STAT Software for PC. Release 6.11, SAS Institute, Cary, NC, USA

Spiegelman, B. M. and J. S. Flier. 1996. Flier, Adipogenesis and obesity: rounding out the big picture. Cell 87:377-389 crossref(new window)

Sugihara, H., N. Yonemitsu, S. Miyabara and S. Toda. 1987. Proliferation of unilocular fat cells in primary culture. J. Lipid Res. 28:1038-1045

Tanaka, S., E. Takahashi, T. Matsui and H. Yano. 2001. Zinc promotes adipocyte differentiation in vitro. Asian-Aust. J. Anim. Sci. 14:966-969

Wood, J. D., S. N. Brown, G. R. Nute, F. M. Whittington, A. M. Perry, S. P. Johnson and M. Enser. 1996. Effects of breed, feed level and conditioning time on the tenderness of pork. Meat Sci. 44:105-112 crossref(new window)