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Low Lysine Treatment Increases Adipogenic Potential of Bovine Intramuscular Preadipocytes
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 Title & Authors
Low Lysine Treatment Increases Adipogenic Potential of Bovine Intramuscular Preadipocytes
Beloor, Jagadish; Kang, Hye Kyeong; Yun, Cheol-Heui; Kim, Sang Hoon; Moon, Yang Soo;
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 Abstract
The molecular mechanism of adipocyte differentiation has been well documented. However, the effect of specific nutrients such as lysine on adipocyte differentiation is poorly understood especially in ruminant animals. Therefore, the aim of the present study was to elucidate the influence of lysine on adipocyte differentiation and adipogenic genes in cultured bovine preadipocytes. The preadipocytes were treated with different concentrations of lysine (40, 160, 320 mg/L) or troglitazone (10 ) for 2 days and then subsequently cultured in differentiation medium until day 6. Expression levels of were significantly higher (p<0.001) in 40 and 160 mg/L lysine-treated cells compared to 320 mg/L treatment. Though there was an increasing trend in expression levels with the decreasing lysine concentration, the results were not significant. The preadipocyte factor (pref-1), expression significantly (p<0.001) reduced with decreasing lysine concentration. The Oil red O staining results were better in 40 mg/L treated cells compared to 160 and 320 mg/L lysine treated cells. Our overall results indicate that insufficient supply of lysine increases the adipogenic potential in bovine intramuscular preadipocytes.
 Keywords
Adipogenesis;Preadipocytes;Lysine;Bovine;
 Language
English
 Cited by
1.
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아세아태평양축산학회지, 2009. vol.22. 9, pp.1320-1327 crossref(new window)
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