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Proteomic Approach Analysis of Mammary Membrane Proteins Expression Profiles in Holstein Cows
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 Title & Authors
Proteomic Approach Analysis of Mammary Membrane Proteins Expression Profiles in Holstein Cows
Yang, Yong-xin; Cao, Sui-zhong; Zhang, Yong; Zhao, Xing-xu;
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 Abstract
To investigate host defense mechanisms for protecting the mammary gland from mastitis infection, the membrane fraction of mammary tissues from Holstein cows was purified by differential velocity centrifugation, and then the sodium dodecyl sulfate-polyacrylamid gel electrophoresis (SDS-PAGE) separated proteins were identified by ion trap mass spectrometer equipped with a Surveyor high performance liquid chromatography (HPLC) system. A total of 183 proteins were identified. Bioinformatics software was applied to analyse physicochemical characteristics of the identified proteins and to predict biochemical function. These data may provide valuable information to investigate the mechanisms of mammary gland milk secretion and infectious disease, and enable a clear identification of proteins and potential protein targets for therapies.
 Keywords
Holstein Cows;Mammary Gland;Proteome;Bioinformatics Software;
 Language
English
 Cited by
 References
1.
Baeker, R., S. Haebel, K. Schlatterer and B. Schlatterer. 2002. Lipocalin-type prostaglandin D synthase in milk: a new biomarker for bovine mastitis. Prostaglandins Other Lipid Mediat. 67(1):75-88 crossref(new window)

2.
Benz, J. and A. Hofmann. 1997. Annexins: from structure to function. Biol. Chem. 378(3-4):177-183 crossref(new window)

3.
Butt, R. H. and J. R. Coorssen. 2005. Postfractionation for enhanced proteomic analyses: routine electrophoretic methods increase the resolution of standard 2D-PAGE. J. Proteome Res. 4(3):982-991 crossref(new window)

4.
Churchward, M. A., R. H. Butt, J. C. Lang, K. K. Hsu and J. R. Coorssen. 2005. Enhanced detergent extraction for analysis of membrane proteomes by two-dimensional gel electrophoresis. Proteome Sci. 3(1):5 crossref(new window)

5.
Cole, A. M., Y. H. Kim, S. Tahk, T. Hong, P. Weis, A. J. Waring and T. Ganz. 2001. Calcitermin, a novel antimicrobial peptide isolated from human airway secretions. FEBS. Lett. 504(1-2):5-10 crossref(new window)

6.
Daniels, K. M., K. E. Webb, Jr., M. L. McGilliard, M. J. Meyer, M. E. Van Amburgh and R. M. Akers. 2006. Effects of body weight and nutrition on mammary protein expression profiles in Holstein heifers. J. Dairy Sci. 89(11):4276-4288 crossref(new window)

7.
Darling, D. L., J. Yingling and A. Wynshaw-Boris. 2005. Role of 14-3-3 proteins in eukaryotic signaling and development. Curr. Top. Dev. Biol. 68:281-315 crossref(new window)

8.
Fountoulakis, M. and L. Suter. 2002. Proteomic analysis of the rat liver. J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 782(1-2):197-218 crossref(new window)

9.
Hogarth, C. J., J. L. Fitzpatrick, A. M. Nolan, F. J. Young, A. Pitt and P. D. Eckersall. 2004. Differential protein composition of bovine whey: a comparison of whey from healthy animals and from those with clinical mastitis. Proteomics 4(7):2094-2100 crossref(new window)

10.
Kashino, Y. 2003. Separation methods in the analysis of protein membrane complexes. J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 797(1-2):191-216 crossref(new window)

11.
Kim, S. M., M. Y. Park, K. S. Seo, D. H. Yoon, H.-G. Lee, Y. J. Choi and S. H. Kim. 2006. Analysis of differentially expressed proteins in bovine longissimus dorsi and biceps femoris muscles. Asian-Aust. J. Anim. Sci. 19(10):1496-1502

12.
Lehner, I., M. Niehof and J. Borlak. 2003. An optimized method for the isolation and identification of membrane proteins. Electrophoresis 24(11):1795-1808 crossref(new window)

13.
Luche, S., V. Santoni and T. Rabilloud. 2003. Evaluation of nonionic and zwitterionic detergents as membrane protein solubilizers in two-dimensional electrophoresis. Proteomics 3(3):249-253 crossref(new window)

14.
Mitaku, S., M. Ono, T. Hirokawa, S. Boon-Chieng and M. Sonoyama. 1999. Proportion of membrane proteins in proteomes of 15 single-cell organisms analyzed by the SOSUI prediction system. Biophys. Chem. 82(2-3):165-171 crossref(new window)

15.
Moss, S. E. and R. O. Morgan. 2004. The annexins. Genome Biol. 5(4):219 crossref(new window)

16.
Ohsaki, H., M. Okada, S. Sasazaki, T. Hinenoya, T. Sawa, S. Iwanaga, H. Tsuruta, F. Mukai and H. Mannen. 2007. Proteomic comparison between Japanese black and holstein cattle by two-dimensional gel electrophoresis and identification of proteins. Asian-Aust. J. Anim. Sci. 20(5):638-644

17.
Patton, W. F. 1999. Proteome analysis. II. Protein subcellular redistribution: linking physiology to genomics via the proteome and separation technologies involved. J. Chromatogr. B. Biomed. Sci. Appl. 722(1-2):203-223 crossref(new window)

18.
Rabilloud, T., T. Blisnick, M. Heller, S. Luche, R. Aebersold, J. Lunardi and C. Braun-Breton. 1999. Analysis of membrane proteins by two-dimensional electrophoresis: comparison of the proteins extracted from normal or Plasmodium falciparuminfected erythrocyte ghosts. Electrophoresis 20(18):3603-3610 crossref(new window)

19.
Rahbar, A. M. and C. Fenselau. 2004. Integration of Jacobson's pellicle method into proteomic strategies for plasma membrane proteins. J. Proteome Res. 3(6):1267-1277 crossref(new window)

20.
Reinhardt, T. A. and J. D. Lippolis. 2006. Bovine milk fat globule membrane proteome. J. Dairy Res. 73(4):406-416 crossref(new window)

21.
Smolenski, G., S. Haines, F. Y. Kwan, J. Bond, V. Farr, S. R. Davis, K. Stelwagen and T. T. Wheeler. 2007. Characterisation of host defence proteins in milk using a proteomic approach. J. Proteome Res. 6(1):207-215 crossref(new window)

22.
Sordillo, L. M., K. Shafer-Weaver and D. DeRosa. 1997. Immunobiology of the mammary gland. J. Dairy Sci. 80(8): 1851-1865 crossref(new window)

23.
Tzivion, G., V. S. Gupta, L. Kaplun and V. Balan. 2006. 14-3-3 proteins as potential oncogenes. Semin. Cancer Biol. 16(3):203-213 crossref(new window)

24.
van Heusden, G. P. 2005. 14-3-3 proteins: regulators of numerous eukaryotic proteins. IUBMB Life 57(9):623-629 crossref(new window)

25.
Wehmhoner, D., G. Dieterich, E. Fischer, M. Baumgartner, J. Wehland and L. Jansch. 2005. 'LaneSpector', a tool for membrane proteome profiling based on sodium dodecyl sulfate -polyacrylamide gel electrophoresis/liquid chromatographytandem mass spectrometry analysis: application to Listeria monocytogenes membrane proteins. Electrophoresis 26(12):2450-2460 crossref(new window)

26.
Wu, C. C. and J. R. Yates, 3rd. 2003. The application of mass spectrometry to membrane proteomics. Nat. Biotechnol. 21(3):262-267 crossref(new window)

27.
Zhang, L., J. Xie, X. Wang, X. Liu, X. Tang, R. Cao, W. Hu, S. Nie, C. Fan and S. Liang. 2005. Proteomic analysis of mouse liver plasma membrane: use of differential extraction to enrich hydrophobic membrane proteins. Proteomics 5(17):4510-4524 crossref(new window)

28.
Zucht, H. D., M. Raida, K. Adermann, H. J. Magert and W. G. Forssmann. 1995. Casocidin-I: a casein-alpha s2 derived peptide exhibits antibacterial activity. FEBS Lett. 372(2-3):185-188 crossref(new window)