Advanced SearchSearch Tips
Effects of Glucagon-like Peptide-2 on Morphology, Proliferation and Enzyme Activity of Intestinal Enterocyte Cells of Weaned Piglets In vitro
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effects of Glucagon-like Peptide-2 on Morphology, Proliferation and Enzyme Activity of Intestinal Enterocyte Cells of Weaned Piglets In vitro
Jia, Gang; Jiang, RongChuan; Wang, KangNing;
  PDF(new window)
This study was conducted according to the single-factor design principle to investigate in vitro the effects of different glucagon-like peptide-2 (GLP-2) concentrations (0, , , , and mol/L) on the morphology, proliferation and enzyme activity of intestinal enterocyte cells of 28-d-old weaned piglets. These cells were primary cultured in 4 pieces of 24-well cell culture plate. After having been grown for 48 h in culture media with hGLP-2, the ileal enterocyte cells of 28-d-old weaned piglets exhibited the typical characteristics of simple columnar epithelium. Compared with the control groups, the quantities of treated cells significantly increased (p<0.05) and their corresponding absorption values in 540 nm (MTT OD) also significantly increased (p<0.01). Likewise, lactic acid concentration, total protein content and protein retention significantly increased (p<0.05). , -ATP enzyme activity was more active (p<0.05), although the activity of alkaline phosphatase, lactic acid dehydrogenase and creatine phosphokinase in culture media significantly decreased (p<0.01). To summarize, the results indicated that GLP-2 in vitro is capable of promoting the proliferation of intestinal enterocyte cells of 28-d weaned piglets, restraining their apoptosis and maintaining the integrity of their morphology.
Glucagon-like Peptide-2, Weaned Piglets;Intestinal Enterocyte Cell;Cell Proliferation;Enzyme Activity;
 Cited by
Benjamin, M. A., D. M. McKay, P. C. Yang, H. Cameron and M. H. Perdue. 2000. Glucagon-like peptide-2 enhances intestinal epithelial barrier function of both transcellular and paracellular pathways in the mouse. Gut. 47:112-119 crossref(new window)

Boushey, R. P., B. Yusta and D. J. Drucker. 2001. Glucagon-like peptide (GLP)-2 reduces chemotherapy associated mortality and enhances cell survival in cells expressing a transfected GLP-2 receptor. Cancer Res. 61:687-693

Burrin, D. G.., B. Stoll, R. Jiang, Y. Petersen, J. Elnif, R. K. Buddington, M. Schmidt, J. J. Holst, B. Hartmann and P. T. Sangild. 2000. GLP-2 stimulates intestinal growth in premature TPN-fed pigs by suppressing proteolysis and apoptosis. Am. J. Physiol. Gastr. L. Physiol. 279:G1249-G1256

Burrin, D. G., B. Stoll and X. F. Guan. 2003. Glucagon-like peptide 2 function in domestic animals. Domes. Anim. Endocrin. 23:103-122 crossref(new window)

Burrin, D. G., B. Stoll, X. F. Guan, L. W. Cui, X. Y. Chang and J. J. Holst. 2005. Glucagon-like peptide 2 dose-dependently activates intestinal cell survival and proliferation in neonatal piglets. Endocrinol. 146:(1)22-32 crossref(new window)

Cottrell, J. J., B. Stoll, R. K. Buddington, J. E. Stephens, L. Cui, X. Chang and D. G. Burrin. 2006. Glucagon-like peptide-2 protects against TPN-induced intestinal hexose malabsorption in enterally refed piglets. Am. J. Physiol. Gastr. L. Physiol. 290:2(1),G293-G300

Estall, J. L. and D. J. Drucker. 2003. Dual regulation of cell proliferation and survival via activation of Glucagon-like peptide-2 receptor signaling. J. Nutr. 133:3708-3711

Estall, J. L., B. Yusta and D. J. Drucker. 2004. Lipid raftdependent Glucagon-like peptide-2 receptor trafficking occurs independently of agonist-induced desensitization. Mol. Biol. Cell. 15:3673-3687 crossref(new window)

Evans, G. S., N. Flint and A. S. Somers. 1992. The development of a method for the preparation of rat intestinal cell primary cultures. J. Cell Sci. 101:219-231 crossref(new window)

Han, S. Y., M. K. Gupta, S. J. Uhm and H. T. Lee. 2009. Isolation and in vitro culture of pig spermatogonial stem cell. Asian-Aust. J. Anim. Sci. 22:187-193

Hartmann, B., J. Thulesen, H. Kissow, S. Thulesen, C. Orskov, C. Ropke, S. S. Poulsen and J. J. Holst. 2000. Dipeptidyl peptidase IV inhibition enhances the intestinotrophic effect of glucagon-like peptide-2 in rats and mice. Endocrinol. 141:4013-4020 crossref(new window)

He, Y. P., S. H. W. Chu and W. A. Walker. 1993. Nucleotide supplements alter proliferation and differentiation of cultured human (Caco-2) and rat (IEC-6) intestinal epithelial cells. J. Nutr. 123:1017-1027

Kelly, C. M., P. Tyers, M. Borg, C. N. Svendsen, S. B. Dunnett and A. E. Rosser. 2005. EGF and FGF-2 responsiveness of rat and mouse neural precursors derived from the embryonic CNS. Brain Res. Bull. 68:83-98 crossref(new window)

Kim, Y. G., J. D. Lohakare, J. H. Yun, S. Heo and B. J. Chae. 2007. Effect of feeding levels of microbial fermented soy protein on the growth performance, nutrient digestibility and intestinal morphology in weaned piglets. Asian-Aust. J. Anim. Sci. 20:399-404

Koldovsky, O., J. Britton, J. Grimes and P. Schaudies. 1991. Milkborne epidermal growth factor (EGF) and its processing in developing gastrointestinal tract. Endocrine Regulations 25:58-62

Martin, G. R., L. E. Wallace and D. L. Sigalet. 2004. Glucagonlike peptide-2 induces intestinal adaptation in parenterally fed rats with short bowel syndrome. Am. J. Physiol. Gastr. L. Physiol. 286:G964-G972 crossref(new window)

Munroe, D. G., A. K. Gupta, F. Kooshesh, T. B. Vyas, G. Rizkalla, H. Wang, L. Demchyshyn, Z. J. Yang, R. K. Kamboj, H. Chen, K. McCallum, M. Sumner-Smith, D. J. Drucker and A. Crivich. 1999. Prototypic G-protein-coupled receptor for the intestinotrophic factor glucagon-like peptide 2. Proc. Natl. Acad. Sci. USA. 96:1569-1573 crossref(new window)

Nielsen, T. T., P. T.Sangild, J. Elnif, K. Sorensen, T. Leser, J. J. Holst, B. Hartmann, B. B. Jensen and M. S. Hedemann. 2003. Effects of GLP-2 treatment and antibiotics on gut structure and function during pig weanling diarrhea. In: Proceedings of the 9th International Symposium on Digestive Physiology in Pigs. Banff, Alberta, Canada. pp. 161-163

Pedersen, N. B., K. R. Hjollund, A. H. Johnsen, C. Orskov, M. M. Rosenkilde, B. Hartmann and J. J. Holst. 2008. Porcine glucagon-like peptide-2: structure, signaling, metabolism and effects. Regul. Peptides. 146:310-320 crossref(new window)

Petersen, Y. M., D. G. Burrin, M. Schmidt, B. Hartmann, J. J. Holst and P. T. Sangild. 2001. Glucagon-like peptide 2 has differential effects on small intestinal growth and function in fetal and neonatal pigs. Am. J. Physiol. Regul. Integr. Comp. Physiol. 281:R1986-R1993

Petersen, Y. M., J. Elnif, M. Schmidt and P. T. Sangild. 2002. Glucagon-like peptide 2 enhances maltase-glucoamylase and sucrase-isomaltase gene expression and activity in parenterally fed premature neonatal piglets. Pediatr. Res. 52:498-503

Petersen, Y. M., B. Hartmann, J. J. Holst, I. L. Huerou-Luron, C. R. Bj$\o$rnvad and P. T. Sangild. 2003. Introduction of enteral food increases plasma GLP-2 and decreases GLP-2 receptor mRNA abundance during pig development. J. Nutr. 133:1781-1786

Sangild, P. T., K. A. Tappenden, C. Malo, Y. M. Petersen, J. Elnif, A. L.Bartholome and R. K. Buddington. 2006. Glucagon-like peptide 2 stimulates intestinal nutrient absorption in parenterally fed newborn pigs. J. Pediatr. Gastr. Nutr.. 43:160-167 crossref(new window)

Sangild, P. T., C. Malo, M. Schmidt, Y. M. Petersen, J. Elnif, J. J. Holst and R. K. Buddington. 2007. Glucagon-like peptide 2 has limited efficacy to increase nutrient absorption in fetal and preterm pigs. Am. J. Physiol. Regul. Integr. Comp. Physiol. 293:R2179-R2184 crossref(new window)

Sigalet, D. L., O. Bawazir, G. R. Martin, L. E. Wallace, G. Zaharko, A. Miller and A. Zubaidi. 2006. Glucagon-like peptide-2 induces a specific pattern of adaptation in remnant jejunum. Digest. Dis. Sci. 51:1557-1566 crossref(new window)

Velazquez, E., J. M. Ruiz-Albusac and E. Blazquez. 2003. Glucagon-like peptide-2 stimulates the proliferation of cultured rat astrocytes. Eur. J. Biochem. 270:3001-3009 crossref(new window)

Washizawa, N., L. H. Gu, L. Gu, K. P. Openo, D. P. Jones and T. R. Ziegler. 2004. Comparative effects of glucagon-like peptide-2 (GLP-2), growth hormone (GH), and keratinocyte growth factor (KGF) on markers of gut adaptation after massive small bowel resection in rats. JPEN-J Parenter Enter. Nutr. 28:399-409 crossref(new window)

Yang, Y. X., Y. G. Kim, J. D. Lohakare, J. H. Yun, J. K. Lee, M. S. Kwon, J. I. Park, J. Y. Choi and B. J. Chae. 2007. Comparative efficacy of different soy protein sources on growth performance, nutrient digestibility and intestinal morphology in weaned pigs. Asian-Aust. J. Anim. Sci. 20:775-783