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Effect of Nicotinamide on Proliferation, Differentiation, and Energy Metabolism in Bovine Preadipocytes
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 Title & Authors
Effect of Nicotinamide on Proliferation, Differentiation, and Energy Metabolism in Bovine Preadipocytes
Liu, Xiaomu; Fu, Jinlian; Song, Enliang; Zang, Kun; Wan, Fachun; Wu, Naike; Wang, Aiguo;
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This study examined the effects of nicotinamide on proliferation, differentiation, and energy metabolism in a primary culture of bovine adipocytes. After treatment of cells with 100-500 nicotinamide, cell growth was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and cellular lipid content was assessed by Oil Red O staining and a triglyceride (TG) assay. Several factors related to energy metabolism, namely adenosine triphosphatase (ATPase) activity, nitric oxide (NO) content, nitric oxide synthase (NOS) activity, the number of mitochondria and the relative expression of glyceraldehydes-3-phosphate dehydrogenase (GAPDH), peroxisome proliferator-activated receptor- () and inducible NOS (iNOS), were also investigated. Results showed that nicotinamide induced both proliferation and differentiation in bovine preadipocytes. Nicotinamide decreased NO production by inhibiting NOS activity and iNOS mRNA expression, and controlled lipolytic activity by increasing ATPase activity and the number of mitochondria. The present study provides further evidence of the effects of nicotinamide on lipid and energy metabolism, and suggests that nicotinamide may play an important role in the development of bovine adipose tissue in vivo. This emphasizes the importance of investigating bovine adipose tissue to improve our understanding of dairy cow physiology.
Nicotinamide;Bovine Preadipocytes;Nitric Oxide;Triglyceride;Lipolysis;Energy Balance;
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Andrade, J., M. Conde, R. Ramirez, J. Monteseirin, J. Conde, F. Sobrino and F. J. Bedoya. 1996. Protection from nicotinamide inhibition of interleukin-1 beta-induced rin cell nitric oxide formation is associated with induction of mnsod enzyme activity. Endocrinology.137(11):4806-4810 crossref(new window)

Bai, L., W. J. Pang, Y. J. Yang and G. S. Yang. 2008. Modulation of sirt1 by resveratrol and nicotinamide alters proliferation and differentiation of pig preadipocytes. Mol. Cell. Biochem. 307(1-2):129-140 crossref(new window)

Barak, Y., M. C. Nelson, E. S. Ong, Y. Z. Jones, P. Ruiz-Lozano, K. R. Chien, A. Koder and R. M. Evans. 1999. Ppar gamma is required for placental, cardiac, and adipose tissue development. Mol. Cell 4(4):585-595 crossref(new window)

Barroso, I., B. Benito, C. Garci-Jimenez, A. Hernandez, M. J. Obregon and P. Santisteban. 1999. Norepinephrine, triiodothyronine and insulin upregulate glyceraldehyde-3-phosphate dehydrogenase mrna during brown adipocyte differentiation. Eur. J. Endocrinol. 141:169-179 crossref(new window)

Beloor, J., H. K. Kang, C. Yun, S. H. Kim and Y. S. Moon. 2009. Low lysine treatment increases adipogenic potential of bovine intramuscular preadipocytes. Asian-Aust. J. Anim. Sci. 22(5):721-726

Bossy-Wetzel, E. and S. A. Lipton. 2003. Nitric oxide signaling regulates mitochondrial number and function. Cell Death Differ. 10(7):757-760 crossref(new window)

Campbell, J. M., M. R. Murphy, R. A. Christensen and T. R. Overton. 1994. Kinetics of niacin supplements in lactating dairy cows. J. Dairy Sci. 77:566-575 crossref(new window)

Catalan, V., J. Gomez-Ambrosi, F. Rotellar, C. Silva, A. Rodriguez, J. Salvador, M. J. Gil, J. A. Cienfuegos and G. Fruhbeck. 2007. Validation of endogenous control genes in human adipose tissue: Relevance to obesity and obesity-associated type 2 diabetes mellitus. Horm. Metab. Res. 39(7):495-500 crossref(new window)

Cetkovic-Cvrlje, M., S. Sandler and D. L. Eizirik. 1993. Nicotinamide and dexamethasone inhibit interleukin-1-induced nitric oxide production by rinm5f cells without decreasing messenger ribonucleic acid expression for nitric oxide synthase. Endocrinology.133(4):1739-1743 crossref(new window)

Collins, P. B. and S. Chaykin. 1972. The management of nicotinamide and nicotinic acid in the mouse. J. Biol. Chem. 247(3):778-783

Engeli, S., J. Janke, K. Gorzelniak, J. Bohnke, N. Ghose, C. Lindschau, F. C. Luft and A. M. Sharma. 2004. Regulation of the nitric oxide system in human adipose tissue. J. Lipid Res. 45(9):1640-1648 crossref(new window)

Goff, J. P. and R. L. Horst. 1997. Physiological changes at parturition and their relationship to metabolic disorders. J. Dairy Sci. 80(7):1260-1268 crossref(new window)

Hauner, H. 2005. Secretory factors from human adipose tissue and their functional role. Proc. Nutr. Soc. 64(2):163-169 crossref(new window)

Hauschildt, S., P. Scheipers, W. G. Bessler and A. Mulsch. 1992. Induction of nitric oxide synthase in l929 cells by tumournecrosis factor alpha is prevented by inhibitors of poly (adpribose) polymerase. Biochem. J. 288( Pt 1):255-260

Heuer, C., Y. H. Schukken and P. Dobbelaar. 1999. Postpartum body condition score and results from the first test day milk as predictors of disease, fertility, yield, and culling in commercial dairy herds. J. Dairy Sci. 82(2):295-304 crossref(new window)

Hungate, R. E. 1966. The rumen and its microbes. Academic Press, New York

Jaster, E. H., D. F. Bell and T. A. Mcpherron. 1983. Nicotinic acid and serum metabolite concentrations of lactating dairy cows fed supplemental niacin. J. Dairy Sci. 66:1039-1045 crossref(new window)

Jaster, E. H. and N. E. Ward. 1990. Supplemental nicotinic acid or nicotinamide for lactating dairy cows. J Dairy Sci. 73(10):2880-2887 crossref(new window)

Jeong, Y. H., S. M. Lee, H. Kim, H. Y. Park, D. Yoon, S. J. Moon, A. Hosoda, D. Kim, S. Saeki and M. Kang. 2008. Cloning, expression, and regulation of bovine cellular retinoic acidbinding Protein-II (CRABP-II) during adipogenesis. Asian-Aust. J. Anim. Sci. 21(11):1551-1558

Kaanders, J. H., L. A. Pop, H. A. Marres, I. Bruaset, F. J. van den Hoogen, M. A. Merkx and A. J. van der Kogel. 2002. Arcon: Experience in 215 patients with advanced head-and-neck cancer. Int. J. Radiat. Oncol. Biol. Phys. 52(3):769-778 crossref(new window)

Komatsu, T., F. Itoh, S. Mikawa and K. Hodate. 2003. Gene expression of resistin in adipose tissue and mammary gland of lactating and non-lactating cows. J. Endocrinol. 178(3):R1-5 crossref(new window)

Kronfeld, D. S. and F. Raggi. 1964. Nicotinamide coenzyme concentrations in mammary biopsy samples from ketotic cows. Biochem. J. 90(1):219-224

Lehnert, S. A., A. Reverter, K. A. Byrne, Y. Wang, G. S. Nattrass, N. J. Hudson and P. L. Greenwood. 2007. Gene expression studies of developing bovine longissimus muscle from two different beef cattle breeds. BMC Dev. Biol. 7:95-108 crossref(new window)

Lewis, J. E., Y. Shimizu and N. Shimizu. 1982. Nicotinamide inhibits adipocyte differentiation of 3t3-l1 cells. FEBS Lett. 146(1):37-41 crossref(new window)

Li, F., Z. Z. Chong and K. Maiese. 2006. Cell life versus cell longevity: The mysteries surrounding the nad+ precursor nicotinamide. Curr. Med. Chem. 13(8):883-895 crossref(new window)

Lin, S. J. and L. Guarente. 2003. Nicotinamide adenine dinucleotide, a metabolic regulator of transcription, longevity and disease. Curr. Opin. Cell Biol. 15(2):241-246 crossref(new window)

Liu, Y. M., J. M. Lacorte, N. Viguerie, C. Poitou, V. Pelloux, B. Guy-Grand, C. Coussieu, D. Langin, A. Basdevant and K. Clement. 2003. Adiponectin gene expression in subcutaneous adipose tissue of obese women in response to shortterm very low calorie diet and refeeding. J. Clin. Endocrinol. Metab. 88:5881-5886 crossref(new window)

Livak, K. J. and T. D. Schmittgen. 2001. Analysis of relative gene expression data using real-time quantitative pcr and the 2(-delta delta c(t)) method. Methods.25(4):402-408 crossref(new window)

Magni, G., A. Amici, M. Emanuelli, G. Orsomando, N. Raffaelli and S. Ruggieri. 2004. Enzymology of nad+ homeostasis in man. Cell. Mol. Life Sci. 61(1):19-34 crossref(new window)

Medina-Gomez, G., S. Gray and A. Vidal-Puig. 2007. Adipogenesis and lipotoxicity: Role of peroxisome proliferator-activated receptor gamma (ppargamma) and ppargammacoactivator-1 (pgc1). Public Health Nutr. 10(10A):1132-1137 crossref(new window)

Morrison, R. F. and S. R. Farmer. 2000. Hormonal signaling and transcriptional control of adipocyte differentiation. J. Nutr. 130(12):3116S-3121S

Nisoli, E., E. Clementi, C. Paolucci, V. Cozzi, C. Tonello, C. Sciorati, R. Bracale, A. Valerio, M. Francolini, S. Moncada and M. O. Carruba. 2003. Mitochondrial biogenesis in mammals: The role of endogenous nitric oxide. Science.299(5608):896-899 crossref(new window)

Oikarinen, A., J. Makela, T. Vuorio and E. Vuorio. 1991. Comparison on collagen gene expression in the developing chick embryo tendon and heart. Tissue and development timedependent action of dexamethasone. Biochim. Biophys. Acta. 1089:40-46 crossref(new window)

Otonkoski, T., G. M. Beattie, M. I. Mally, C. Ricordi and A. Hayek. 1993. Nicotinamide is a potent inducer of endocrine differentiation in cultured human fetal pancreatic cells. J. Clin. Invest. 92(3):1459-1466 crossref(new window)

Pellat-Deceunynck, C., J. Wietzerbin and J. C. Drapier. 1994. Nicotinamide inhibits nitric oxide synthase mrna induction in activated macrophages. Biochem. J. 297( Pt 1):53-58

Pires, J. A. and R. R. Grummer. 2007. The use of nicotinic acid to induce sustained low plasma nonesterified fatty acids in feedrestricted holstein cows. J. Dairy Sci. 90(8):3725-3732 crossref(new window)

Rolland, V., I. Dugail, X. L. Liepvre and M. Lavau. 1995a. Evidence of increased glyceraldehyde-3-phosphate dehydrogenase and fatty acid synthetase promoteractivities in transiently transfected adipocytes from genetically obese rats. J. Biol. Chem. 270:1102-1106 crossref(new window)

Rolland, V., X. L. Liepvre, M. L. Houbiguian, M. Lavau and I. Dugail. 1995b. C/EBP alpha expression in adipose tissue of genetically obese zucker rats. 207:761-767 crossref(new window)

Choi, K. C., Y. B. Shrestha, S. G. Roh, D. Hishikawa, M. Kuno, H. Tsuzuki, Y. H. Hong and S. Sasaki. 2003. The role of phosphatidylinositol 3-kinase and mitogenic activated protein kinase on the differentiation of ovine preadipocytes. Asian-Aust. J. Anim. Sci. 16(8):1199-1204

Rosen, E. D., P. Sarraf, A. E. Troy, G. Bradwin, K. Moore, D. S. Milstone, B. M. Spiegelman and R. M. Mortensen. 1999. PPAR gamma is required for the differentiation of adipose tissue in vivo and in vitro. Mol. Cell 4(4):611-617 crossref(new window)

Schmittgen, T. D. and K. J. Livak. 2008. Analyzing real-time pcr data by the comparative ct method. Nat. Protoc. 3(6):1101-1108 crossref(new window)

Sirover, M. 1999. New insights into an old protein: The functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase. Biochem. Biophy. Acta. 432:159-184

Stanley, S., K. Wynne, B. McGowan and S. Bloom. 2005. Hormonal regulation of food intake. Physiol. Rev. 85(4):1131-1158 crossref(new window)

Talke, H., K. P. Maier, M. Kersten and W. Gerok. 1973. Effect of nicotinamide on carbohydrate metabolism in the rat liver during starvation. Eur. J. Clin. Invest. 3(6):467-474 crossref(new window)

Ulbrich, S. E., S. Rehfeld, S. Bauersachs, E. Wolf, R. Rottmayer, S. Hiendleder, M. Vermehren, F. Sinowatz, H. H. Meyer and R. Einspanier. 2006. Region-specific expression of nitric oxide synthases in the bovine oviduct during the oestrous cycle and in vitro. J. Endocrinol. 188(2):205-213 crossref(new window)

Vaca, P., G. Berna, F. Martin and B. Soria. 2003. Nicotinamide induces both proliferation and differentiation of embryonic stem cells into insulin-producing cells. Transplant. Proc. 35(5):2021-2023 crossref(new window)

Williams, A. and D. Ramsden. 2005. Nicotinamide: A double edged sword. Parkinsonism Relat. Disord. 11(7):413-420 crossref(new window)

Yan, H., E. Aziz, G. Shillabeer, A. Wong, D. Shanghavi, A. Kermouni, M. Abdel-Hafez and D. C. Lau. 2002. Nitric oxide promotes differentiation of rat white preadipocytes in culture. J. Lipid Res. 43(12):2123-2129 crossref(new window)

Yang, J., L. K. Klaidman, A. Nalbandian, J. Oliver, M. L. Chang, P. H. Chan and J. D. J. Adams. 2002. The effects of nicotinamide on energy metabolism following transient focal cerebral ischemia in wistar rats. Neurosci. Lett. 333(2):91-94 crossref(new window)

Yang, J., S. P. Zhao, J. Li and S. Z. Dong. 2008. Effect of niacin on adipocyte leptin in hypercholesterolemic rabbits. Cardiovasc. Pathol. 17(4):219-225 crossref(new window)

Yu, B.-L., Z. Shui-Ping, X. Xiao-Zhu and S. Z. Dong. 2007. Effect of niacin on cholesterol efflux in 3T3-L1 cells. Chinese Journal of Arteriosclerosis.15(4):289-292