Advanced SearchSearch Tips
Intermediary Metabolism of Plasma Acetic Acid, Glucose and Protein in Sheep Fed a Rice Straw-based Diet
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Intermediary Metabolism of Plasma Acetic Acid, Glucose and Protein in Sheep Fed a Rice Straw-based Diet
Alam, M.K.; Ogata, Y.; Sako, Y.; Al-Mamun, M.; Sano, H.;
  PDF(new window)
The present study was conducted to determine plasma acetate, glucose and protein metabolism using dilution of isotopes [[1-]Na acetate, [U-]glucose and [1-]leucine (Leu)] in sheep fed rice straw (Oriza japonica L.). Four sheep were assigned to either rice straw (RS-diet) or mixed hay (MH-diet) with a crossover design. Nitrogen (N) intake and N digestibility were lower (p = 0.002 and p = 0.02, respectively) for RS-diet than MH-diet, but N retention did not differ (p>0.10) between the diets. Concentrations of rumen acetate tended to be lower (p = 0.07), and propionate was higher (p = 0.02) for RS-diet than MH-diet. Concentrations of plasma lactate, non-esterified fatty acids, Leu and -ketoisocaproic acid did not differ (p>0.10) between the diets, but plasma glucose and urea concentrations were lower (p = 0.01 and p = 0.003, respectively) for RS-diet than MH-diet. Turnover rate of plasma acetate did not differ (p = 0.39) between the diets, and plasma glucose and Leu turnover rates were numerically lower (p = 0.15 and p = 0.14, respectively) for RS-diet than MH-diet. Whole body protein synthesis and degradation did not differ (p>0.10) between the diets. Thus it can be concluded that the intermediary metabolism of acetate, glucose and protein on rice straw is comparable to mixed hay in sheep.
Rice Straw;Intermediary Metabolism;Mixed Hay;Stable Isotope;Sheep;
 Cited by
Effects of Chinese herbal medicine on plasma glucose, protein and energy metabolism in sheep, Journal of Animal Science and Biotechnology, 2013, 4, 1, 51  crossref(new windwow)
Effects of Rice Straw Supplemented with Urea and Molasses on Intermediary Metabolism of Plasma Glucose and Leucine in Sheep, Asian-Australasian Journal of Animal Sciences, 2016, 29, 4, 523  crossref(new windwow)
Acorda, J. A., M. Okamoto and N. Yoshida. 1992. Nutritive value of rice straw processed with sodium hydroxide, soybean meal, urea, cage layer manure and molasses. J. Jpn. Soc. Grassl. Sci. 37:405-411.

Al-Mamun, M., K. Goto, S. Chiba and H. Sano. 2009. Responses of plasma acetate metabolism to hop (Humulus lupulus L.) in sheep. Int. J. Biol. Sci. 5:287-292. crossref(new window)

Al-Mamun, M., Y. Hanai, C. Tanaka, Y. Tamura and H. Sano. 2008. Responses of whole body protein synthesis and degradation to plantain herb in sheep exposed to heat. Arch. Anim. Nutr. 62:219-229. crossref(new window)

Al-Mamun, M., C. Ito, A. Sato, T. Fujita and H. Sano. 2007. Comparison of the [$^2H_5$]phenylalanine model with [$1-^{13}C$]leucine method to determine whole body protein synthesis and degradation in sheep fed two levels. Asian-Aust. J. Anim. Sci. 20:1517-1524. crossref(new window)

AOAC. 1995. Official methods of analysis. 16th edn. Association of Official Analytical Chemists, Arlington, Virginia.

Calder, A. G. and A. Smith. 1988. Stable isotope ratio analysis of leucine and ketoisocaproic acid in blood plasma by gas chromatography/mass spectrometry. Use of tertiary butyldimethylsilyl derivatives. Rapid Commun. Mass Spectrom. 2:14-16. crossref(new window)

Castillo, A. R., E. Kebreab, D. E. Beever, J. H. Barbi, J. D. Sutton,H. C. Kirby and J. France. 2001. The effect of protein supplementation on nitrogen utilization in lactating dairy cows fed grass silage diets. J. Anim. Sci. 79:247-253.

Chilliard, Y., A. Feraly, Y. Faulconnier, M. Bonnet, J. Rouel and F. Bocquier. 2000. Adipose tissue metabolism and its role in adaptations to undernutrition in ruminants. Proc. Nutr. Soc. 59:127-134. crossref(new window)

Evans, E., J. G. Buchanan-Smith, G. K. MacLeod and J. B. Stone. 1974. Glucose metabolism in cows fed low and high-roughage diets. J. Dairy Sci. 58:672-677.

Fujita, T., M. Kajita and H. Sano. 2006. Responses of whole body protein synthesis, nitrogen retention and glucose kinetics to supplemental starch in goats. Comp. Biochem. Physiol. B. 144:180-187.

Fujita, T., M. Kajita and H. Sano. 2007. Effects of non protein energy intake on whole body protein synthesis, nitrogen retention and glucose turnover in goats. Asian-Aust. J. Anim. Sci. 20:536-542. crossref(new window)

Harris, P. M., P. A. Skene, V. Buchan, E. Milne, A. G. Calder, S. E.Anderson, A. Connell and G. E. Lobely. 1992. Effect of food intake on hind-limb and whole body protein metabolism in young growing sheep: Chronic studies based on arterio-venous techniques. Br. J. Nutr. 68:389-407. crossref(new window)

Hossain, M. S., M. N. Haque, S. A. Aziz, M. S. Mazumder, M. L.Ali, and A. T. M. M-E-Elahi. 2002. Effect of urea molasses straw on the productive and reproductive performance of indigenous cows under the village conditions of Bangladesh. Pakistan J. Biol. Sci. 9:997-999.

Huggett, A. G. and D. A. Nixon. 1957. Enzymatic determination of blood glucose. Biochem. J. 66:12.

Krishnamurti, C. R. and S. M. Janssens. 1988. Determination of leucine metabolism and protein turnover in sheep, using gasliquid chromatography-mass spectrometry. Br. J. Nutr. 59:155-164. crossref(new window)

Konig, B. A., J. D. Oldham and D. S. Parker. 1984. The effect of abomasal infusion of casein on acetate, palmitate and glucose kinetics in cows during early lactation. Br. J. Nutr. 52:319-328. crossref(new window)

Lapierre, H., J. P. Blouin, J. F. Bernier, C. K. Reynolds, P.Dubreuil and G. E. Lobley. 2002. Effect of supply of metabolizable protein on whole body and splanchnic leucine metabolism in lactating cows. J. Dairy Sci. 48:193-198.

Moreau, N. M., S. M. Goupry, J. P. Antiganac, F. J. Monteau, B. J.Le Biezec, M. M. Champ, L. J. Martin and H. J. Dumon. 2003.Simultaneous measurement of plasma concentrations and $^{13}C$-enrichment of short-chain fatty acids, lactic acid and ketone bodies by gas chromatography coupled to mass spectrometry. J. Chromatogr. B. 784:395-403. crossref(new window)

National Agriculture and Food Research Organization. 2006. Japanese feeding standard for dairy cattle. Japan Livestock Industry Association.

National Research Council. 1985. Nutrient requirements of sheep. 6th Ed. National Academy Press. Washington, DC.

Ortigues-Marty, I., J. Vernet and L. Majdoub. 2003. Whole body glucose turnover in growing and non-productive adult ruminants: meta-analysis and review. J. Reprod. Nutr. Dev. 43:371-383. crossref(new window)

Pethick, D. W. and D. B. Lindsay. 1982. Acetate metabolism in lactating sheep. Br. J. Nutr. 48:319-328. crossref(new window)

Prior, R. L. 1978. Effect of level of feed intake on lactate and acetate metabolism and lipogenesis in vivo in sheep. J. Nutr. 108:926-935.

Rocchiccioli, F., J. P. Leroux and P. Cartier. 1981. Quantitation of 2-ketoacids in biological fluids by gas chromatography chemical ionization mass spectrometry of otrimethylsilylquinoxalinol derivatives. Biomed. Mass Spectrom. 8:160-164. crossref(new window)

Rodriguez, N. R., E. C. Prigge, D. C. Lough and W. H. Hoover.1985. Glucogenic and hormonal responses to abomasal casein and ruminal volatile fatty acid infusions in lactating goats. J. Dairy Sci. 68:1968-1975. crossref(new window)

Salman, F. M., R. I. El-Kadi, H. Abdel-Rahman, S. M. Ahmed, M. I. Mohammad and M. M. Shoukry. 2008. Biologically treated sugar beet pulp as a supplement in goat rations. Int. J. Agric. Biol. 10:412-416.

Sano, H. and T. Fujita. 2006. Effect of supplemental calcium propionate on insulin action to blood glucose metabolism in adult sheep. Reprod. Nutr. Dev. 46:9-18. crossref(new window)

Sano, H., T. Fujita, M. Murakami and A. Shiga. 1996. Stimulative effect of epipherine on glucose production and utilization rates in sheep using a stable isotope. Domest. Anim. Endocrionol. 13:445-451. crossref(new window)

Sano, H., M. Kajita and T. Fujita. 2004. Effect of dietary protein intake on plasma leucine flux, protein synthesis, and degradation in sheep. Comp. Biochem. Physiol. B. 139:163-168. crossref(new window)

Sano, H., D. N. Mowat, R. O. Ball and D. R. Trout. 1997. Effect of supplemental chromium on whole-body kinetics of glucose, lactate and propionate in rams fed a high grain diet. Comp. Biochem. Physiol. B. 118:117-121. crossref(new window)

Sano, H., H. Sawada, A. Takenami and M. Al-Mamun. 2009. Effects of diet and cold exposure on rates of plasma leucine turnover and protein synthesis in sheep. J. Agric. Sci. (Camb.) 147:91-97. crossref(new window)

Sano, H., A. Takebayashi, Y. Kodama, K. Nakamura, H. Ito, Y.Arino, T. Fujita, H. Takahashi and K. Ambo. 1999. Effects of feed restriction and cold exposure on glucose metabolism in response to feeding and insulin in sheep. J. Anim. Sci. 77:2564-2573.

Sano, H., Y. Tamura and A. Shiga. 2002. Metabolism and glucose kinetics in sheep fed plantain and orchard grass and exposed to cold. NZ. J. Agric. Res. 45:171-177. crossref(new window)

Santoso, B., B. Mwenya, C. Sar and J. Takahashi. 2006. Ruminal fermentation and nitrogen metabolism in sheep fed a silagebased diet supplemented with Yucca schidigera and nisin. Anim. Feed Sci. Technol. 129:187-195. crossref(new window)

SAS. 1996. $SAS/STAT^{\circledR}$ Software: Changes and Enhancements through Release 6.11. SAS Inst. Inc. Cary, NC.

Schroeder, G. F., E. C. Titgemeyer, M. S. Awadeh, J. S. Smith and D. P. Gnad. 2006. Effects of energy level on methionine utilization by growing steers. J. Anim. Sci. 84:1497-1504.

Sticker, L. S., D. L. Thompson, L. D. Bunting, J. M. Fernandez and C. L. DePew. 1995. Dietary protein and (or) energy restriction in mares: plasma glucose, insulin, nonesterified fatty acid and urea nitrogen responses to feeding, glucose and epinephrine. J. Anim. Sci. 73:136-144.

Sunagawa, K., F. Otani, A. Hagino, K. Takahashi, Y. Otomo, Y.Shoji, K. Ambo and T. Tsuda. 1986. Kinetics of exogenous and endogenous acetate metabolism in sheep exposed to cold environment. Jpn. J. Zootech. Sci. 57:201-208.

Sutton, J. D., M. S. Dhanoa, S. V. Morant, J. France, D. J. Napperand E. Schuller. 2003. Rates of production of acetate, propionate and butyrate in the rumen of lactating dairy cows given normal and low roughage diets. J. Dairy Sci. 86:3620-3633. crossref(new window)

Tserng, K. Y. and S. C. Kalhan. 1983. Calculation of substrate turnover rate in stable isotope tracer studies. Am. J. Physiol. 245:E308-E311.

Van Soest, P. J. 2006. Rice straw, the role of silica and treatments to improve quality. J. Anim. Feed Sci. Technol. 130:137-171. crossref(new window)

Weatherburn, M. W. 1967. Phenol-hypochlorite reaction for determination of ammonia. J. Anal. Chem. 39:971-974. crossref(new window)

Wu, Y., W. Hu and J. Liu. 2005. Effects of supplementary ureamineral lick block on the kinetics of fiber digestion, nutrient digestibility and nitrogen utilization of low quality roughages. J. Zhejiang Univ. Sci. 8:793-797.