Advanced SearchSearch Tips
The Effects of Propolis on Biochemical Parameters and Activity of Antioxidant Enzymes in Broilers Exposed to Lead-Induced Oxidative Stress
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
The Effects of Propolis on Biochemical Parameters and Activity of Antioxidant Enzymes in Broilers Exposed to Lead-Induced Oxidative Stress
Seven, Ismail; Aksu, Taylan; Seven, Pinar Tatli;
  PDF(new window)
This study aimed to determine the effects of vitamin C and propolis-supplemented feeds on some blood parameters, lipid peroxidation, and activities of some antioxidant enzymes in broilers exposed to oxidative stress. 360 three-day-old broiler chicks (Ross 308) were randomly divided into four treatment groups each containing 90 animals, including six replicate groups for each treatment. The experimental groups were designated for a 3-42 days period as follows: no supplement to basal ration (Control-Group I); supplement of 500 ppm vitamin C and 200 ppm lead (as lead acetate) to basal ration (Group II); supplement of 1 g/kg propolis and 200 ppm lead (as lead acetate) to basal ration (Group III); and supplement of 200 ppm lead (as lead acetate) to basal ration (Group IV). The highest TG level (86.83 mg/dl) was observed in the lead supplemented group; however, the lowest aspartate aminotransferase (SGOT) level (90.71 IU/L) was observed in the control group (p<0.05). The addition of lead increased the plasma malondialdehyde (MDA) level (p<0.01) compared to other treatments. However, the addition of vitamin C and propolis decreased the plasma MDA level close to control levels. The highest erythrocyte superoxide dismutase (SOD) activity was observed in the lead addition group (p<0.01) while no significant differences were observed for SOD activities of the control, vitamin C +lead, and propolis+lead groups. The plasma reduced glutathione (GSH) activity of the control (/ml) was significantly lower than the lead administered group (/ml) (p<0.01); while this parameter was determined to be similar to other groups. No significant differences were observed between groups for liver GSH activity, but heart GSH activity of the control was significantly higher in comparison to other treatments (p<0.05). To obtain similar antioxidant effects, it is recommend that using propolis (1 g/kg) and vitamin C (500 mg/kg) supplementation in broiler diets may overcome the adverse effects of oxidative stress originating from dietary lead.
Broiler;Lead;Oxidative Stress;Propolis;Blood;Malondialdehyde;Antioxidant Enzymes;
 Cited by
Effect of Dietary Sodium Nitrate Consumption on Egg Production, Egg Quality Characteristics and Some Blood Indices in Native Hens of West Azarbaijan Province,Safary, H.;Daneshyar, Mohsen;

Asian-Australasian Journal of Animal Sciences, 2012. vol.25. 11, pp.1611-1616 crossref(new window)
The Influence of Propolis as Supplement Diet on Broiler Meat Growth Performance, Carcass Body Weight, Chemical Composition and Lipid Oxidation Stability, Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 2015, 63, 2, 411  crossref(new windwow)
Effect of dietary supplementation with an ethanolic extract of propolis on broiler intestinal morphology and digestive enzyme activity, Journal of Animal Physiology and Animal Nutrition, 2014, 98, 2, 393  crossref(new windwow)
Antioxidant Properties of Chinese Propolis in Ross Broilers Exposed to Heat Stress in Egypt, Open Journal of Veterinary Medicine, 2015, 05, 09, 197  crossref(new windwow)
The effects of propolis and vitamin C supplemented feed on performance, nutrient utilization and carcass characteristics in broilers exposed to lead, Livestock Science, 2012, 148, 1-2, 10  crossref(new windwow)
Functions of propolis as a natural feed additive in poultry, World's Poultry Science Journal, 2016, 72, 01, 37  crossref(new windwow)
The effects of propolis on serum malondialdehyde, fatty acids and some blood parameters in Japanese quail (Coturnix coturnix japonica) under high stocking density, Journal of Applied Animal Research, 2017, 45, 1, 417  crossref(new windwow)
Effects of Dietary Lycopene Supplementation on Plasma Lipid Profile, Lipid Peroxidation and Antioxidant Defense System in Feedlot Bamei Lamb, Asian-Australasian Journal of Animal Sciences, 2015, 28, 7, 958  crossref(new windwow)
Effect of Saponin on the Phytoextraction of Pb, Cd and Zn from Soil Using Italian Ryegrass, Bulletin of Environmental Contamination and Toxicology, 2015, 94, 1, 129  crossref(new windwow)
Intestinal morphology and activity of digestive enzymes in broilers fed crude propolis, Canadian Journal of Animal Science, 2014, 94, 1, 105  crossref(new windwow)
Effect of Dietary Lead on Intestinal Nutrient Transporters mRNA Expression in Broiler Chickens, BioMed Research International, 2015, 2015, 1  crossref(new windwow)
Effect of Dietary Sodium Nitrate Consumption on Egg Production, Egg Quality Characteristics and Some Blood Indices in Native Hens of West Azarbaijan Province, Asian-Australasian Journal of Animal Sciences, 2012, 25, 11, 1611  crossref(new windwow)
Aebi, H. 1984. Catalase in vitro methods in enzymology (Ed. B. J. Willam), Academic Press, New York, ABD, 105:121-126.

Ahamed, M. and M. K. J. Siddiqui. 2007. Environmental lead toxicity and nutritional factors. Clin. Nutr. 26:400-408. crossref(new window)

Akgul, E., N. Ilhan, I. Halifeoglu, N. Ihan and A. Var. 1997. Plasma lipid peroxide levels and antioxidant enzyme activities in type II diabetics: Relationship with diabetic microagiopatic complications. Atherosclerosis 130:9.

Aliciguzel, Y., I. Ozen, M. Aslan and U. Karayalcin. 2003. Activities of xanthine oxidoreductase and antioxidant enzymes in different tissues of diabetic rats. J. Lab. Clin. Med. 142:172-177. crossref(new window)

Altan, O., A. Pabuccuoglu, A. Altan, S. Konyalioglu and H. Bayraktar. 2003. Effect of heat stress on oxidative stress, lipid peroxidation and some stress parameters in broilers. Br. Poult. Sci. 44:545-550. crossref(new window)

AOAC. 1995. Official methods of analysis, 16th ed. Association of Official Analytical Chemists, Arlington, VA, 4.1-4.17.

Asayama, K., H. Hayashibe, K. Dobashi, T. Niitsu, A. Miyao amdK. Kato. 1989. Antioxidant enzyme status and lipid peroxidation in various tissues of diabetic and starved rats. Diabetes Res. 12:85-91.

Ates, B., M. I. Dogru, M. Gul, A. Erdogan, A. K. Dogru, I. Yilmaz,M. Yurekli and M. Esrefoglu. 2006. Protective role of caffeic acid phenethyl ester in the liver of rats exposed to cold stress. Fundam. Clin. Pharmacol. 20:283-289. crossref(new window)

Bains, B. S. 1996. The role of vitamin C in stress management. Missset World Poultry 12:38-41.

Bendich, A. 1993. Physiological role of antioxidants in the immune system. J. Dairy Sci. 76:2789-2794. crossref(new window)

Biavatti, M. W., M. H. Bellaver, L. Volpato, C. Costa and C.Bellaver. 2003. Preliminary studies of alternative feed additives for broilers: Alternanthera Brasiliana Extract, Propolis Extract and Linseed Oil. Rev. Bras. Cienc. Avic. 5:147-151.

Blonska, M., J. Bronikowska, G. Pietsz, Z. P. Czuba, S. Schellerand W. Krol. 2004. Effects of ethanol extract of propolis (EEP) and its flavones on inducible gene expression in J774A.1 Makrophages. J. Ethnopharmacol. 91:25-30. crossref(new window)

Burdock, G. A. 1998. Review of the biological properties and toxicity of bee propolis. Food Chem. Toxicol. 36:347-363. crossref(new window)

Ellman, G. L. 1959. Tissue sulfhydryl groups. Arch. Biochem. Biophys. 82:70-77. crossref(new window)

Erdogan, Z., S. Erdogan, T. Aksu and E. Baytok. 2004. The Effects of dietary lead exposure and ascorbic acid on performance, lipid peroxidation status and biochemical parameters of broilers. Turk J. Vet. Anim. Sci. 29:1053-1059.

Halliwell, B. and J. M. Gutteridge. 1986. Oxygen free radicals and iron in relation to biology and medicine: some problems and concepts. Arch. Biochem. Biophys. 2:501-514.

Havsteen, B. H. 2002. The biochemistry and medical significance of the flavonoids. Pharmacol. Ther. 96:67-202. crossref(new window)

Hosnuter, M., A. Gurel, O. Babuccu, F. Armutcu, E. Kargi and A.Isikdemir. 2004. The effect of CAPE on lipid peroxidation and nitric oxide levels in the plasma of rats following thermal injury. Burs. 30:121-125.

Hsu, P. C., C. C. Hsu, M. Y. Liu, L. Y. Chen and Y. L. Guo. 1998. Lead-induced changes in spermatozoa function and metabolism. J. Toxicol. Environ. Health 55:45-64. crossref(new window)

Huang, W. C., S. W. Juang, I. M. Liu, T. C. Chi and J. T. Cheng.1999. Changes of superoxide dismutase gene expression and activity in the brain of streptozotocin-induced diabetic rats. Neurosci. Lett. 5:25-28.

Ichikawa, H., K. Satoh, T. Tobe, I. Yasuda, F. Ushio, K. Matsumoto, K. Endo and C. Ookubo. 2002. Free radical scavenging activity of propolis. Redox Rep. 7:347-50. crossref(new window)

Irmak, M. K., E. Fadıllıoglu, S. Sogut, H. Erdogan, M. Gulec, M.Ozer, M. Yagmurca and M. E. Gozukara. 2003. Effects of caffeic acid phenethyl ester and alpha-tocopherol on reperfusion injury in rat brain. Cell Biochem. Funct. 21:283-289. crossref(new window)

Kimoto, N., M. Hirose, M. Kawabe, T. Satoh, H. Miyataka and T.Shirai. 1999. Post-initiation effects of a super critical extract of propolis in a rat two-stage carcinogenesis model in female F344 rats. Cancer Lett. 147:221-227. crossref(new window)

Lowry, O. H., N. J. Rosebrough, A. L. Farr and R. J. Randall. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193:265-275.

Lysenko, M. 2005. Unithiol - the means preventing lead accumulation in tissues and organs of broiler. Conference Information: Proceedings of the XVII European Symposium on the Quality of Poultry Meat and XI European Symposium on the Quality of Eggs and Egg Products, Golden Tulip Parkhotel Doorwerth, Doorwerth, Netherlands, 23-26 May 2005.

NRC. 1994. Nutrient requirements of poultry, National Research Council, National Academy Press, Washington, DC.

Ohkawa, H., N. Ohishi and K. Yagi. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95:351-358. crossref(new window)

Ohtsuka, A., H. Kojima, T. Ohtani and K. Hayashi. 1998. Vitamin E reduces glucocorticoid-induced oxidative stress in rat skeletal muscle. J. Nutr. Sci. Vitam. 44:779-786. crossref(new window)

Okonenko, L. B., B. B. Aidarkhanov, A. A. Rakhmetova, SSh.Zhakisheva and ZhS. Iksymbaeva. 2006. Vitamin E and propolis as antioxidants after excessive administration of polyunsaturated fatty acids. Fundam. Clin. Pharmacol. 20:283-289. crossref(new window)

Okutan, H., N. Ozcelik, H.Y. Ramazan and U. Efkan. 2005. Effects of caffeic acid phenethyl ester on lipid peroxidation and antioxidant enzymes in diabetic rat heart. Clin. Biochem. 38:191-196. crossref(new window)

Orzechowski, O., P. Ostaszewski, A. Brodnicka, J. Wilczak, M.Jank, B. Balasinska, K. Grzelkowska, T. Ploszaj, J. Olczak andA. Mrowczynska. 2000. Excess of glucocorticoids impairs whole-body antioxidant status in young rats. Relation to the effect of dexamethasone in soleus muscle and spleen. Horm. Metab. Res. 32:174-180. crossref(new window)

Ozkaya, Y. G., A. Agar and P. Yargicoglu. 2002. The effect of exercise on brain antioxidant status of diabetic rats. Diabetes Metab. 28:377-384.

Padmavathi, R., P. Senthilnathan, D. Codon and D. Sakthisekaran.2006. Therapeutic effect of paclitaxel and propolis on lipid peroxidation and antioxidant system in 7,12 Dimethyl Benz(a) Anthracene-Induced Breast Cancer in female sprague dawley rats. Life Sci. 78:2820-2825. crossref(new window)

Patra, R. C., D. Swarup and S. K. Dwidedi. 2001. Antioxidant Effects of $\alpha$-tocopherol, ascorbic acid and l-methionine on lead-induced oxidative stress of the liver, kidney and brain in rats. Toxicology 162:81-88. crossref(new window)

Placer, Z. A., L. L. Cushman and B. C. Johnson. 1966. Estimation of product of lipid peroxidation (Malonyl Dialdehyde) in biochemical systems. Anal. Biochem. 16:359-364. crossref(new window)

Prytzyk, E., A. P. Dantas, K. Salomão, A. S. Pereira, V. S. Bankova, S. L. De Castro and F. R. Aquino Neto. 2003. Flavonoids and trypanocidal activity of bulgarian propolis. J Ethnopharmacol. 88:189-193. crossref(new window)

Sies, H. 1991. Oxidative stress: from basic research to clinical application. Am. J. Med. 91:31-38.

SPSS. 1999. $SPSS^{\circledR}$ for Windows Base System User’s Guide, Release 11.5, USA.

Stefano, C. and C. Francesco. 2002. Propolis an old remedy used in modern medicine. Fitoterapia 73:1-6. crossref(new window)

Tatli Seven, P. 2008. The effects of dietary Turkish propolis and vitamin C on performance, digestibility, egg production and egg quality in laying hens under different environmental temperatures. Asian-Aust. J. Anim. Sci. 21:1164-1170. crossref(new window)

Tatli Seven, P. and I. Seven. 2008 The effect of dietary Turkish propolis as alternative to antibiotic on performance and digestibility in broilers exposed to heat stress. J. Appl. Anim. Res. 34:193-196. crossref(new window)

Tatli Seven, P., I. Seven, M. Yilmaz and G. Simsek. 2008. The effects of Turkish propolis on growth and carcass characteristics in broilers under heat stress. Anim. Feed Sci. Technol. 146:137-148. crossref(new window)

Tatli Seven, P., S. Yilmaz, I. Seven, I. H. Cerci, M. A. Azman andM. Yilmaz. 2009. The effect of propolis on selected blood indicators and antioxidant enzyme activities in broilers under heat stress. Acta Vet. Brno 78:75-83. crossref(new window)

Wang, B. J., Y. H. Lien and Z. R. Yu. 2004. Supercritical fluid extractive fractionation: study of the antioxidant activities of propolis. Food Chem. 86:237-243. crossref(new window)

Wohaieb, S. A. and D. V. Godin. 1987. Alterations in free radical tissue-defence mechanisms in streptozocin-induced diabetes in rat. Effects of insulin treatment. Diabetes 36:1014-1018.

Yıldırım, O. and Z. Buyukbingol. 2003. Effect of cobalt on the oxidative status in heart and aorta of streptozotocin-induced diabetic rats. Cell Biochem. Funct. 21:27-33. crossref(new window)

Yousef, M. I., T. I. Awad and E. H. Mohamed. 2006. Deltamethrininduced oxidative damage and biochemical alterations in rat and its attenuation by vitamin E. Toxicology 227:240-247. crossref(new window)

Zaidi, S. M., T. M. Al-Qirim and N. Banu. 2005. Effects of antioxidant vitamins on glutathione depletion and lipid peroxidation induced by restraint stress in the rat liver. Drugs R. D. 6:157-165. crossref(new window)