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Garlic (Allium sativum) Supplementation: Influence on Egg Production, Quality, and Yolk Cholesterol Level in Layer Hens
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 Title & Authors
Garlic (Allium sativum) Supplementation: Influence on Egg Production, Quality, and Yolk Cholesterol Level in Layer Hens
Mahmoud, Kamel Z.; Gharaibeh, Saad M.; Zakaria, Hana A.; Qatramiz, Amer M.;
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Forty-eight 40-wk-old Hi-sex laying hens were individually caged in an environmentally controlled house to evaluate the effect of garlic (Allium Sativum) juice administration on egg production, egg quality, and yolk cholesterol. Garlic juice was prepared by blending pealed garlic cloves with distilled water (1:1, w/w). Hens were randomly divided into four equal groups; one served as a control and the other three groups were individually gavaged, 3.75 ml, 7.5 ml, or 15 ml garlic juice, three times a week, which respectively represented 0.25, 0.50 and 1% of body weight. Egg production was recorded on a daily basis; egg weight, albumen height, albumen and yolk pH, Haugh unit, and bacterial count of E. coli-challenged eggs were recorded at day of oviposition (day-1) and after 5 and 10 days of storage at room temperature. Yolk cholesterol content was analyzed for five successive weeks. Garlic juice increased (p<0.05) egg weight and mass with no change in egg production intensity. Garlic juice administration recorded higher (p<0.05) albumen height and improvement in Haugh unit. Also, eggs from garlic-treated hens recorded lower (p<0.05) albumen and yolk pH when compared to eggs collected from control hens. Garlic reduced (p<0.05) the of bacterial count in egg contents linearly when challenged with E. coli. Egg-yolk cholesterol content was not influenced by garlic juice administration. It is concluded that garlic juice improved performance characteristics and may increase egg shelf life as indicated by egg quality improvement and lower bacterial count of E. coli-challenged eggs. The levels of garlic juice used in this study were insufficient to influence egg yolk cholesterol.
Garlic;Hen;Egg;Bacterial Count;Cholesterol;
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아세아태평양축산학회지, 2015. vol.28. 4, pp.538-543 crossref(new window)
Garlic (Allium sativum) supplementation in poultry diets: effect on production and physiology, World's Poultry Science Journal, 2012, 68, 03, 417  crossref(new windwow)
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Effect of Olive Leaf (Olea europaea) Powder on Laying Hens Performance, Egg Quality and Egg Yolk Cholesterol Levels, Asian-Australasian Journal of Animal Sciences, 2015, 28, 4, 538  crossref(new windwow)
Effects of Volatile Oil of Garlic on Feed Utilization, Blood Biochemistry and Performance of Heat-stressed Japanese Quail, Asian Journal of Poultry Science, 2017, 11, 2, 83  crossref(new windwow)
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Adibmoradi, M., B. Navidshad, J. Seifdavati and M. Royan. 2006.Effect of dietary garlic meal on histological structure of small intestine in broiler chickens. Jpn. Poult. Sci. 43:378-383. crossref(new window)

Ahn, D. U., J. L. Sel, C. Jo, M. Chamruspollert and M. Jeffrey.1999. Effect of dietary conjugated linoleic acid on the quality characteristics of chicken eggs during refrigerated storage. Poult. Sci. 78:922-928. crossref(new window)

Ankri, S. and D. Mirelman. 1999. Antimicrobial properties of allicin from garlic. Microbes Infect. 2:125-129.

AOAC. 1990. Official methods of analysis, 15th ed. Assoc. Ana. Chem., Arlington, VA.

Berthold, H. K., T. Sudhop and K. Von Bergmann. 1998. Effects of a garlic oil preparation on serum lipoproteins and cholesterol metabolism. J. Am. Med. Assoc. 279:1900-1902. crossref(new window)

Birrenkott, G., G. E. Brockenfel, M. Owens and E. Halpin. 2000. Yolk and blood cholestrol levels and organolptic assement of eggs from hens feds a garlic supplemented diet. Poult. Sci. 79(Suppl. 1):75(Abstr.).

Block, E. 1985. The chemistry of garlic and onion. Sci. Am. 252: 114-119.

Chowdhury, S. R., S. D. Chowdhury and T. K. Smith. 2002. Effects of dietary garlic on cholesterol metabolism in laying hens. Poult. Sci. 81:1856-1862. crossref(new window)

Guo, F. C., B. A. Williams, R. P. Kwakkel, H. S. Li, X. P. Li, J. Y.Luo, W. Li and M. W. Verstegen. 2004. Effects of mushroom and herb polysaccharides as alternatives for an antibiotic on the cecal microbial ecosystem in broiler chicks. Poult. Sci. 83:175-182. crossref(new window)

Gurler, M. and K. Fehlhaber. 2004. Growth of Salmonella enteritidis in yolk from eggs laid by immunized hens. Int. J. Food Microbiol. 90:107-113. crossref(new window)

Hargis, P. S. 1988. Modifying egg yolk cholesterol in domestic fowl-A review. Worlds Poult. Sci. J. 44:17-29. crossref(new window)

Harris, J. C., S. L. Cottrell, S. Plummer and D. Lloyd. 2001. Antimicrobial properties of Allium sativum (garlic). Appl. Microbiol. Biotechnol. 57:282-286. crossref(new window)

Haugh, R. R. 1937. Haugh unit for measuring egg quality. US Egg Poult. Mag. 43:522-555.

Huang, T. C., C. P. Chen, V. Wefler and A. Raftery. 1961. A stable reagent for the libermann-Burchard reaction. Anal. Chem. 33:1405-1407. crossref(new window)

Kasuga, S., N. Uda, E. Kyo, M. Ushijima, N. Morihara and Y.Itakura. 2001. Pharmacologic activities of aged garlic extract in comparison with other garlic preparations. J. Nutr. 131: 1080-1084.

Khan, S. H., R. Sardar and M. Anjum. 2007. Effects of dietary garlic on performance and serum and egg yolk cholesterol concentration in laying hens. Asian J. Poult. Sci. 1:22-27. crossref(new window)

Lawson, L. and B. G. Hughes. 1992. Characterization of the formation of allicin and other thiosulfinates from garlic. Planta Med. 58:345-350. crossref(new window)

Lee, Y. L., T. Cesario, Y. Wang, E. Shanbrom and L. Thrupp. 2003. Antibacterial activity of vegetables and juices. Nutrition 19: 994-996. crossref(new window)

Li-Chan, E. and S. Nakai. 1989. Biochemical basis for the properties of egg white. Crit. Rev. Poult. Biol. 2:21-59.

Lim, K. S., S. J. You, B. K. An and C. W. Kang. 2006. Effects of dietary garlic powder and copper on cholesterol content and quality characteristics of chicken eggs. Asian-Aust. J. Anim. Sci. 19:582-590. crossref(new window)

Marks, H. L. and K. W. Washburn. 1977. Divergent selection for yolk cholesterol in laying hens. Br. Poult. Sci. 18:179-188. crossref(new window)

Monia, K. N., M. Salahuddin and G. Miah. 2003. Effect of breed and holding period on egg quality characteristics of chicken. Int. J. Poult. Sci. 2:261-263. crossref(new window)

Mirunalini, S., R. Kumaraguruparan, R. Subapriya and S. Nagini.2004. Garlic oil enhances hepatic and blood antioxidants during hamster buccal pouch carcinogenesis. Pharm. Biol. 42:240-245. crossref(new window)

Neil, A. and C. Sigaly. 1994. Garlic; its cardio protective properties. Curr. Opin. Lipidol. 5:6-10. crossref(new window)

Nusairat, B. M. 2007. Dietary supplementation of garlic (Allium Sativum): Influence on performance parameters, meat quality and humoral immune response in broiler chicks. M.S. Thesis, Jordan University of Science and Technology, Irbid, Jordan.

Pappas, A. C., T. Acamovic, N. H. Sparks, P. F. Surai and R. M.McDevitt. 2005. Effects of supplementing broiler breeder diets with organic selenium and polyunsaturated fatty acids on egg quality during storage. Poult. Sci. 84:865-874. crossref(new window)

Ramakrishna, R. R., K. Platel and K. Srinivasan. 2003. In vitro influence of spices and spice-active principles on digestive enzymes of rat pancreas and small intestine. Nahrung 47: 408-412. crossref(new window)

Reddy, R. V., S. F. Lightsey and D. V. Maurice. 1991. Effect of feeding garlic oil on performance and egg yolk cholesterol concentration. Poult. Sci. 70:2006-2009. crossref(new window)

Reeve, V. E., M. Bosnic, E. Rosinova and C. Boehm-Wilcox. 1993. A garlic extract protects from ultraviolet B (280-320 nm) radiation-induced suppression of contact hypersensitivity. Photochem. Photobiol. 58:813-817. crossref(new window)

Rosso, L., J. R. Lobry, S. Bajard and J. P. Flandrois. 1995.Convenient model to describe the combined effects of temperature and pH on microbial growth. Appl. Environ. Microbiol. 61:610-616.

Saeed, A. M. and C. W. Koons. 1993. Growth and heat resistance of Salmonella enteritidis in refrigerated and abused eggs. J. Food Prot. 56:927-931.

Sarica, S., A. Ciftci, E. Demir, K. Kilinc and Y. Yildirim. 2005.Use of an antibiotic growth promoter and two herbal natural feed additives with and without exogenous enzymes in wheat based broiler diets. S. Afr. J. Anim. Sci. 35:61-72.

SAS Institute Inc. 1996. SAS/STAT user’s guide. SAS Institute Inc., Cary, NC.

Scott, T. A. and F. G. Silversides. 2000. The effect of storage and strain of hen on egg quality. Poult. Sci. 79:1725-1729. crossref(new window)

Sharma, R. K., R. A. Singh, R. N. Pal and C. K. Aggarwal. 1979.Cholesterol content of chicken eggs as affected by feeding garlic, sarpagandha, and nicotinic acid. Haryana Agric. Univ. J. Res. 9:263-265.

Sigaly, C., W. Neil and W. H. Andrew. 1994. A meta-analysis of the effect of garlic on blood pressure. J. Hypertens. 12:463-468.

Sigaly, C. and A. Neil. 1994. Garlic, its cardio-protective properties. Curr. Opin. Lipidol. 5:6-10. crossref(new window)

Silversides, F. G. and T. A. Scott. 2001. Effect of storage and layer age on quality of eggs from two lines of hens. Poult. Sci. 80:1240-1245. crossref(new window)

Silversides, F. G. and K. Budgell. 2004. The relationships among measures of egg albumen height, pH, and whipping volume. Poult. Sci. 83:1619-1623. crossref(new window)

Sivam, G. P. 2001. Protection against Helicobacter pylori and other bacterial infections by garlic. J. Nutr. 131:1106-1108.

Thomson, M. and M. Ali. 2003 Garlic (Allium sativum): a review of its potential use as an anti-cancer agent. Curr. Cancer Drug Tar. 3:67-81. crossref(new window)

Tucker, L. A. 2002. Plant extracts to maintain poultry performance. Feed Inter. 23:26-29.

Vargas, R. E., J. B. Allred, M. D. Biggert and E. C. Neber. 1986. Effect of dietary 7-ketocholesterol, pure, or oxidized cholesterol on hepatic 3-hepatic 3-hydroxy-3-methylgiutarylconenzyme A reductase activity, energy balance, egg cholesterol concentration and 15C-acetate incorporation into yolk lipids of laying hens. Poult. Sci. 65:1333-1342. crossref(new window)

Yalcin, S., I. Onbaslar, A. Sehu and S. Yalcin. 2007. The effect of dietary garlic powder on the performance, egg triats and blood serum cholesterol of laying quails. Asian-Aust. J. Anim. Sci. 20:944-950. crossref(new window)

Yalcin, S., E. E. Onbasilar, Z. Reisli and S. Yalcin. 2006. Effect of garlic powder on the performance, egg traits and blood parameters of laying hens. J. Sci. Food Agric. 86:1336-1339. crossref(new window)

Yang, G. C., M. P. Yasaei and S. W. Page. 1993. Garlic as antioxidant and free radical scavenger. J. Food Drug Anal. 1:357-364.

Yu, T. H., C. M. Wu and Y. C. Liou. 1989. Volatile compounds from garlic. J. Agric. Food Chem. 37:725-730. crossref(new window)