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Eggshell Pigmentation Study in Blue-shelled and White-shelled Ducks
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 Title & Authors
Eggshell Pigmentation Study in Blue-shelled and White-shelled Ducks
Liu, H.C.; Hsiao, M.C.; Hu, Y.H.; Lee, S.R.; Cheng, W.T.K.;
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This study attempted to clarify the difference in eggshell pigmentation between blue-shelled ducks (BSD) and whiteshelled ducks (WSD). The eggshell pigmentation deposition process is discussed. Ultraviolet spectro-photometer and HPLC were used to determine the biliverdin concentration in the shell gland, uterus liquid and eggshell at 6, 12, 18, 20, 23.5 h post-oviposition. The biliverdin concentration in the eggshell and uterus fluid showed significant differences between BSD and WSD, but not in the shell gland. The heme oxygenase activity in the shell gland of both kinds of ducks remained mostly constant during the ovulatory cycle with no variation. The assay of exogenous biliverdin injection into the shell gland antrum in the WSD indicated that exogenous biliverdin could be deposited continuously into the eggshell until the source was exhausted. A layer-by-layer dissolution assay was used to examine the eggshell pigment deposition process. The biliverdin concentration in the first to sixth layers of the eggshell in the BSD was significantly higher than that in the white-shelled counterpart. The blue pigment concentration increased persistently from the 6th layer to the layer. The BSD eggshells did not accumulate a large quantity of biliverdin in the most external layer. They tended to increase the deposition layer by layer. Our results demonstrated that different BSD and WSD eggshell colors were influenced by the amount of biliverdin in the uterus fluid and not determined by the amount of biliverdin in the shell gland. This implies the existence of a mechanism that controls biliverdin transportation from the shell gland into the uterus fluid, thereby playing a key role in regulating duck eggshell color.
Eggshell Pigmentation;Biliverdin;Blue-shelled Duck;Uterus Liquid;
 Cited by
Baird, T., S. E. Solomon and D. R. Tedstone. 1975. Localisation and characterisation of egg shell porphyrin in several avian species. Br. Poult. Sci. 16:201-208 crossref(new window)

Barnett, G. M., M. F. Hudson and K. M. Smith. 1975. Concerning of meso-tetraphenylporphyrin purification. J. Chem. Soc. 14:1041-1043

Chen, D. T., S. R. Lee, Y. H. Hu, C. C. Huang, Y. S. Cheng, C. Tai, J. P. Poivey and R. Rouvier. 2003. Genetic trends for laying traits in the Brown Tsaiya (Anas platyrynchos) selected with restricted genetic selection index. Asian-Aust. J. Anim. Sci. 16(12):1705-1710

Cornelius, C. E. 1991. Bile pigments in fishes: a review. Vet. Clin. Pathol. 20:106-115 crossref(new window)

Johnson, A. L. 2000. Reproduction in the female. In: Sturkie’s avian physiology (Ed. G. C. Whittow). Academic press, USA. pp. 569-596

Gosler, A. G., J. P. Higham and S. J. Reynolds. 2005. Why are birds' eggs speckled? Ecol. Lett. 8(10):1105-1113 crossref(new window)

Kennedy, G. Y. and H. G. Ververs. 1973. Eggshell pigments of the Arauano fowl. Comp. Biochem. Physiol. 44B:11-35

Kennedy, G. Y. and H. G. Ververs. 1975. A survey of avian eggshell pigments. Comp. Biochem. Physiol. 55B:117-123 crossref(new window)

Lang, M. R. and J. W. Wells. 1987. A review of eggshell pigmentation. World's Poult. Sci. J. 43(3):238-246 crossref(new window)

Liu, S. C., J. F. Huang, T. J. Sun, S. R. Lee and C. T. Wang. 1998. The inheritance of blue eggshell in Brown Tsaiya. J. Taiwan Lives. Res. 31(4):373-382

Ma, R. C. S. 1968. The time of release of the luteinizing hormone from the adenohypophysis of laying domestic ducks. Poult. Sci. 47:404-410

Mik$\check{s}$$\acute{i}$k, I., V. Hol$\acute{a}$$\check{n}$ and Z. Deyl. 1994. Quantification and variability of eggshell pigment content. Comp. Biochem. Physiol. 109A:769-772 crossref(new window)

Moreno, J. and J. L. Osorno. 2003. Avian egg colour and sexual selection: does eggshell pigmentation reflect female condition and genetic quality? Ecol. Lett. 6(9):803-806 crossref(new window)

Nakano, T., N. I. Ikawa and L. Ozimek. 2003. Chemical composition of chicken eggshell and shell membranes. Poult. Sci. 82:510-514

Polin, D. 1957. Formation of porphyrin from delta-aminolevulenic acid by uterine and liver tissue from laying hens. Proc. Soc. Exp. Biol. Med. 94:276-279

Poole, H. K. 1965. Spectrophotometric identification of eggshell pigments and timing of supercial pigment deposition in the Japanese quail. Proc. Soc. Exp. Biol. Med. 119:547-551

Ryter, S. W., E. Kvam and R. W. Tyrrell. 2000. Heme oxygenase activity. Methods Mol. Biol. 99:369-391

SAS Institute Inc. 2002. SAS Qualification Tools User's Guide, version 9.1.3 SAS Institute Inc., Cary, North Carolina

Schwartz, S. W., A. Raux, B. A. Schacter, B. D. Stephenson and R. N. Shoffner. 1980. Loss of hereditary uterine protoporphyria through chromosomal rearrangement in mutant Rhode Island Red hens. Int. J. Biochem. 12:935-940 crossref(new window)

Solomon, S. E. 1991. Eggshell colour. In: Egg and eggshell quality. Wolfe publishing Ltd. England. pp. 123-129

Solomon, S. E. 2002. The oviduct in chaos. World's Poult. Sci. J. 58:41-48 crossref(new window)

Tai, C., R. Rouvier and J. P. Poivey. 1989. Genetic parameters of some growth and egg production traits in laying Brown Tsaiya. Genet. Sel. Evol. 21:377-384 crossref(new window)

Wang, X. T., X. M. Deng, C. J. Zhao, J. Y. Li, G. Y. Xu, L. S. Lian and C. X. Wu. 2007. Study of the deposition process of eggshell pigments using an improved dissolution method. Poult. Sci. 86:2236-2238

Warren, D. C. and R. M. Conrad. 1942. Time of pigment deposition in brown shelled hen eggs and in turkey eggs. Poult. Sci. 21:515-520

Wicke, W. 1858. Ueber das pigment in den Eischalen der V$\ddot{o}$gel. Naumannia. 8:393-397

Zhao, R., G. Y. Xu, Z. Z. Liu, X. Y. Li and N. Yang. 2006. A study on eggshell pigmentation: Biliverdin in blue-shelled chickens. Poult. Sci. 85:546-549

Zhao, R., Z. Z. Liu, G. Y. Xu and N. Yang. 2007. Analysis of SNP markers for chicken blue-shelled gene using PCR-SSCP. Chin. J. Agric. Biotech. 4(1):53-56 crossref(new window)