Advanced SearchSearch Tips
Effects of Feeding Solid-state Fermented Rapeseed Meal on Performance, Nutrient Digestibility, Intestinal Ecology and Intestinal Morphology of Broiler Chickens
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effects of Feeding Solid-state Fermented Rapeseed Meal on Performance, Nutrient Digestibility, Intestinal Ecology and Intestinal Morphology of Broiler Chickens
Chiang, G.; Lu, W.Q.; Piao, X.S.; Hu, J.K.; Gong, L.M.; Thacker, P.A.;
  PDF(new window)
This trial was conducted to determine the effects of feeding a diet containing solid-state fermented rapeseed meal on performance, nutrient digestibility, intestinal ecology and intestinal morphology of broiler chickens. A mixed liquid culture, containing approximately 5 log cfu/ml Lactobacillus fermentum, Enterococcus faecium, Saccharomyces cerevisae and Bacillus subtilis was prepared in a 1:1:1:1 ratio. A basal substrate (BS) containing 75% rapeseed, 24% wheat bran and 1% brown sugar was mixed with the liquid culture in a ratio of 10:3. Over the 30-day fermentation, isothiocyanates were reduced from 119.6 to 14.7 mmol/kg. A total of 168, day-old male Arbor Acres broiler chicks were assigned to one of three dietary treatments including a corn-soybean meal based control diet as well as two experimental diets in which the control diet was supplemented with 10% of the BS containing unfermented rapeseed meal or 10% of the BS containing rapeseed meal subjected to solid state fermentation. There were 8 pens per treatment and 7 birds per pen. From days 19-21 and days 40-42, uncontaminated excreta were collected from each pen for digestibility determinations. In addition, digesta from the colon and ceca were collected to determine the number of lactobacilli, enterobacteria and total aerobes. The middle sections of the duodenum, jejunum, and ileum were collected for intestinal morphology. Over the entire experimental period (d 1-42), the weight gain and feed conversion of birds fed fermented rapeseed meal were superior (p<0.05) to that of birds fed nonfermented rapeseed meal and did not differ from the soybean control. On day 42, birds fed fermented rapeseed meal had higher (p<0.05) total tract apparent digestibility coefficients for dry matter, energy, and calcium than birds fed non-fermented rapeseed meal. Colon and ceca digesta from broilers fed the fermented feed had higher (p<0.05) lactobacilli counts than birds fed the control and non-fermented rapeseed meal diets on day 21 and 42. Fermentation also improved (p<0.05) villus height and the villus height:crypt depth ratio in the ileum and jejunum on day 21 and 42. The results indicate that solid-state fermentation of rapeseed meal enhanced performance and improved the intestinal morphology of broilers and may allow greater quantities of rapeseed meal to be fed to broilers potentially reducing the cost of broiler production.
Broiler;Intestinal Morphology;Nutrient Digestibility;Microbial Characteristics;Rapeseed Meal;Solid-state Fermentation;
 Cited by
Effects of Fermented Potato Pulp on Performance, Nutrient Digestibility, Carcass Traits and Plasma Parameters of Growing-finishing Pigs,;;;;;;

아세아태평양축산학회지, 2011. vol.24. 10, pp.1456-1463 crossref(new window)
Effects of Fermented Soybean Meal on Immune Response of Weaned Calves with Experimentally Induced Lipopolysaccharide Challenge,;;;;;;;;

아세아태평양축산학회지, 2011. vol.24. 7, pp.957-964 crossref(new window)
Effects of Fermented Rapeseed Meal on Growth Performance and Serum Parameters in Ducks,;;;;;;

아세아태평양축산학회지, 2011. vol.24. 5, pp.678-684 crossref(new window)
Effects of Replacement of Soybean Meal by Fermented Cottonseed Meal on Growth Performance, Serum Biochemical Parameters and Immune Function of Yellow-feathered Broilers,;;;;;;

아세아태평양축산학회지, 2012. vol.25. 3, pp.393-400 crossref(new window)
Effects of Replacing Soybean Meal with Fermented Rapeseed Meal on Performance, Serum Biochemical Variables and Intestinal Morphology of Broilers,;;;

아세아태평양축산학회지, 2012. vol.25. 12, pp.1734-1741 crossref(new window)
Energy and Ileal Digestible Amino Acid Concentrations for Growing Pigs and Performance of Weanling Pigs Fed Fermented or Conventional Soybean Meal,;;;;;;;

아세아태평양축산학회지, 2014. vol.27. 5, pp.706-716 crossref(new window)
Effects of dietary inclusion of fermented cottonseed meal on growth, cecal microbial population, small intestinal morphology, and digestive enzyme activity of broilers, Tropical Animal Health and Production, 2013, 45, 4, 987  crossref(new windwow)
The effect of different dietary levels of canola meal on growth performance, nutrient digestibility, and gut morphology of broiler chickens, Poultry Science, 2014, 93, 5, 1130  crossref(new windwow)
leaves on antioxidant capacity, intestinal morphology and microbial ecology in broiler chicks, British Poultry Science, 2015, 56, 3, 370  crossref(new windwow)
Fermented liquid feed for pigs: an ancient technique for the future, Journal of Animal Science and Biotechnology, 2015, 6, 1  crossref(new windwow)
fermented rapeseed meal on nutrient digestibility, growth performance and serum parameters in growing pigs, Animal Science Journal, 2015, 87, 4, 557  crossref(new windwow)
Physicochemical Properties Analysis and Secretome of Aspergillus niger in Fermented Rapeseed Meal, PLOS ONE, 2016, 11, 4, e0153230  crossref(new windwow)
Effects of fermented rapeseed meal on antioxidant functions, serum biochemical parameters and intestinal morphology in broilers, Food and Agricultural Immunology, 2016, 27, 2, 182  crossref(new windwow)
Effect of fermentation of soybean meal with varying protein solubility on ileal digestibility of nutrients in growing pigs, Animal Production Science, 2016, 56, 12, 2023  crossref(new windwow)
Effects of Protease Addition and Replacement of Soybean Meal by Corn Gluten Meal on the Growth of Broilers and on the Environmental Performances of a Broiler Production System in Greece, PLOS ONE, 2017, 12, 1, e0169511  crossref(new windwow)
Effects of soybean meal fermented by L. plantarum, B. subtilis and S. cerevisieae on growth, immune function and intestinal morphology in weaned piglets, Microbial Cell Factories, 2017, 16, 1  crossref(new windwow)
Response of broiler chickens to dietary inclusion of fermented canola meal under heat stress condition, Italian Journal of Animal Science, 2017, 16, 4, 546  crossref(new windwow)
Effects of fermented cottonseed meal on growth performance, serum biochemical parameters, immune functions, antioxidative abilities, and cecal microflora in broilers, Food and Agricultural Immunology, 2017, 28, 4, 725  crossref(new windwow)
Effects of fermented cottonseed meal on the growth performance, gastrointestinal microflora population and small intestinal morphology in broiler chickens, British Poultry Science, 2017, 58, 4, 402  crossref(new windwow)
Solid-state fermentation of corn-soybean meal mixed feed with Bacillus subtilis and Enterococcus faecium for degrading antinutritional factors and enhancing nutritional value, Journal of Animal Science and Biotechnology, 2017, 8, 1  crossref(new windwow)
Adarsh, P. V. and W. Amandeep. 2001. Beneficial effects of Rhizopus oligosporus fermentation on reduction of glucosinolates, fibre and phytic acid in rapeseed (Brassica napus) meal. Bioresour. Technol. 78:309-312 crossref(new window)

AOAC. 1990. Official methods of analysis. 16th ed. Association of Official Analytical Chemists, Washington, DC

Bourdon, D. and A. Aumaitre. 1990. Low glucosinolate rapeseed and rapeseed meal: Effect of technological treatments on chemical composition, digestible energy content and feeding value for growing pigs. Anim. Feed Sci. Technol. 30:175-191 crossref(new window)

Britzman, D. G. 2006. Soybean meal: An excellent protein source for poultry feeds. American Soybean Association Technical Bulletin. Available at Britzman.pdf (Last accessed Nov 20, 2008)

Campbell, L. D. and T. K. Smith. 1979. Response of growing chicks to high dietary content of rapeseed meal. Br. Poult. Sci. 20:231-237 crossref(new window)

Canibe, N. and B. B. Jensen. 2003. Fermented and non-fermented liquid feed to growing pigs: Effect on aspects of gastrointestinal ecology and growth performance. J. Anim. Sci. 81:2019-2031

Canibe, N., E. Virtanen and B. B. Jensen. 2006. Microbial and nutritional characteristics of pig liquid feed during fermentation. Anim. Feed Sci. Technol. 134:108-123

Canibe, N., H. Miettinen and B. B. Jensen. 2008. Effect of adding Lactobacillus plantarum or a formic acid containing product to fermented liquid feed on gastrointestinal ecology and growth performance of piglets. Livest. Sci. 144:251-262

Cheng, D. L., K. Hosimoto and Y. Uda. 2004. In vitro digestion of sinigrin and glucotropeolin by single strain of Bifidobacterium and identification of digestive products. Food Chem. Toxicol. 42:351-357 crossref(new window)

Choi, M. M. F., S. S. Shuang, H. Y. Lai, S. C. Cheng, R. C. W. Cheng, B. K. B. Cheung and W. M. Lee. 2004. Gas chromatography-mass spectrometric determination of total isothiocyanates in Chinese medicinal herbs. Anal. Chem. Acta. 516:155-163 crossref(new window)

Clandinin, D. R., L. Bayley and A. Camballero. 1966. Effect of (${\pm}$)-5-vinyl-2-oxazolididinethione-A toxic content in rapeseed meal, on the rate of growth and thyroid function of chicks. Poult. Sci. 45:833-838

Elangovan, A. V. S., V. S. Verma, V. R. B. Sastry and S. D. Singh. 2001. Effect of feeding high glucosinolate rapeseed meal to laying Japanese Quail. Asian-Aust. J. Anim. Sci. 14:1304-1307

Guo, X. H., D. F. Li, W. Q. Lu, X. S. Piao and X. L. Chen. 2006. Screening of Bacillus strains as potential probiotics and subsequent confirmation of the in vivo effectiveness of Bacillus subtilis MA139 in pigs. Int. J. Gener. Mol. Microbiol. 90:139-146 crossref(new window)

Huang, C., S. Qiao, D. Li, X. Piao and J. Ren. 2004. Effects of lactobacilli on the performance, diarrhea incidence, VFA concentration and gastrointestinal microbial flora of weaning pigs. Asian-Aust. J. Anim. Sci. 17:401-409

Koneman, E. W., S. D. Allen, V. R. Dowell, Jr. and H. M. Sommers. 1983. Color atlas and textbook of diagnostic microbiology. 2nd Ed. J. B. Lippincott Company. Philadelphia, PA. pp. 348-349

Li, D. F., R. C. Thaler, J. L. Nelssen, D. L. Harmon, G. L. Allee and T. L. Weeden. 1990. Effects of fat sources and combinations on starter pig performance, nutrient digestibility and intestinal morphology. J. Anim. Sci. 68:3694-3704

McNeill, L., K. Bernard and M. G. MacLeod. 2004. Food intake, growth rate, food conversion and food choice in broilers fed on diets high in rapeseed meal and pea meal with observations of the resulting poultry meat. Br. Poult. Sci. 45:519-523 crossref(new window)

Mieke, T. 2007. Oil World Annual. ISTA. Mielk GmbH, Hamburg, Germany

Mithen, R. F., M. Dekker, R. Verkerk, S. Rabot and I. T. Johnson. 2000. The nutritional significance, biosynthesis and bioavailability of glucosinolates in human foods. J. Sci. Food Agric. 80:967-984 crossref(new window)

Mueller, W. 1956. Feasibility of the chromic oxide and lignin indicator methods for metabolism experiments with chickens. J. Nutr. 58:29-36

NRC. 1994. Nutrient requirements of poultry. 9th rev. ed. National Academy Press, Washington, DC

Paton, A. W., R. Morona and J. C. Paton. 2006. Designer probiotics for prevention of enteric infections. Nat. Rev. Microbiol. 4:193-200 crossref(new window)

Pluske, J. R., M. J. Thompson, C. S. Atwood, P. H. Bird, L. H. Williams and P. E. Hartmenn. 1996. Maintenance of villus height and crypt depth, and enhancement of disaccharide digestion and monosaccharide absorption, in piglets fed on cows' whole milk after weaning. Br. J. Nutr. 76:409-422 crossref(new window)

Rozan, P., C. Villaume, H. M. Bau, A. Schwert, J. P. Nicolas and I. Mejean. 1996. Detoxification of rapeseed meal by Rhizopus oligosporus sp. T3: A first step towards rapeseed protein concentrate. Ind. J. Food Sci. Technol. 31:85-90 crossref(new window)

SAS Institute. 1996. SAS user's guide: Statistics. Version 7.0. SAS Institute, Cary, NC

Sauer, W. C. and K. de Lange. 1992. Novel methods for determining protein and amino acid digestibilities in feedstuffs. In: Modern methods in protein nutrition and metabolism (Ed. S. Nissen). Academic Press Inc., San Diego, CA. pp. 87-120

Skrede, G., S. Sahlstrøm, A. Skrede, A. Holck and E. Slinde. 2001. Lactic acid fermentation of wheat and barley whole meal flour modifies carbohydrate composition and increases digestibility in mink (Mustela vison). Anim. Feed Sci. Technol. 90:199-212 crossref(new window)

Skrede, G., T. Storebakken, A. Skrede, S. Sahlstrøm, M. Sørensen, K. D. Shearer and E. Slinde. 2002. Lactic acid fermentation of wheat and barley whole meal flours improves digestibility of nutrients and energy in Atlantic salmon (Salmo salar L.) diets. Aquaculture 210:305-321 crossref(new window)

Tripathi, M. K. and A. S. Mishra. 2006. Glucosinolates in animal nutrition: A review. Anim. Feed Sci. Technol. 132:1-27 crossref(new window)

van Winsen, R. L., B. A. P. Urlings, L. J. A. Lipman, J. M. A. Snijders, D. Keuzenkamp, J. H. M. Verheijden and F. van Knapen. 2001. Effect of fermented feed on the microbial population of the gastrointestinal tracts of pigs. Appl. Environ. Microbiol. 67:3071-3076 crossref(new window)

Vig, A. P. and A. Walia. 2001. Beneficial effects of Rhizopus oligosporus fermentation on reduction of glucosinolates, fiber and phytic acid in rapeseed (Brassica napus) meal. Bioresour. Technol. 78:309-312 crossref(new window)

Xu, Z. R., C. H. Hu, M. S. Xia, X. A. Zhan and M. Q. Wang. 2003. Effects of dietary fructooligosaccharide on digestive enzyme activities, intestinal microflora and morphology of male broilers. Poult. Sci. 82:648-654

Zhang, W., Z. Xu, J. Sun and X. Yang. 2006. A study on the reduction of gossypol levels by mixed culture solid substrate fermentation of cottonseed meal. Asian-Aust. J. Anim. Sci. 19:1314-1321