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Molecular Cloning, Tissue Distribution and Expression of Porcine y+L Amino Acid Transporter-1
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 Title & Authors
Molecular Cloning, Tissue Distribution and Expression of Porcine y+L Amino Acid Transporter-1
Zhi, Ai-min; Zhou, Xiang-yan; Zuo, Jian-jun; Zou, Shi-geng; Huang, Zhi-yi; Wang, Xiao-lan; Tao, Lin; Feng, Ding-yuan;
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In this study, we cloned, sequenced and characterized porcine y+L Amino Acid Transporter-1 (y+LAT1). By screening a translated EST database with the protein sequence of the human LAT1 and by using rapid amplification of cDNA ends (RACE), the full-length cDNA encoding porcine LAT1 was isolated from porcine intestine RNA. It was 2,111 bp long, encoding a 511 amino acid trans-membrane glycoprotein composed of 12 transmembrane domains. The predicted amino acid sequence was found to be 91%, 90%, 87% and 87% identical to those of cattle, human, mouse and rat LAT1 respectively. Real-time RT-PCR results indicated that the small intestine had the highest LAT1 mRNA abundance and the lung had the lowest LAT1 mRNA abundance. Baby hamster kidney (BHK) cells transfected with green fluorescent protein (GFP) tagged porcine LAT1 cDNA indicated that the cellular localization of the gene product in BHK was on the plasma membrane.
Cloning;Pig;Amino Acid Transporter;LAT1;SLC7A7;
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