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Differentiated Human Embryonic Stem Cells Enhance the In vitro and In vivo Developmental Potential of Mouse Preimplantation Embryos
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 Title & Authors
Differentiated Human Embryonic Stem Cells Enhance the In vitro and In vivo Developmental Potential of Mouse Preimplantation Embryos
Kim, Eun-Young; Lee, Keum-Sil; Park, Se-Pill;
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In differentiating human embryonic stem (d-hES) cells there are a number of types of cells which may secrete various nutrients and helpful materials for pre-implantation embryonic development. This study examined whether the d-hES could function as a feeder cell in vitro to support mouse embryonic development. By RT-PCR analysis, the d-hES cells revealed high expression of three germ-layered differentiation markers while having markedly reduced expression of stem cell markers. Also, in d-hES cells, LIF expression in embryo implantation-related material was confirmed at a similar level to undifferentiated ES cells. When mouse 2PN embryos were cultured in control M16 medium, co-culture control CR1aa medium or co-cultured with d-hES cells, their blastocyst development rate at embryonic day 4 (83.9%) were significantly better in the d-hES cell group than in the CR1aa group (66.0%), while not better than in the M16 group (90.7%)(p<0.05). However, at embryonic days 5 and 6, embryo hatching and hatched-out rates of the dhES cell group (53.6 and 48.2%, respectively) were superior to those of the M16 group (40.7 and 40.7%, respectively). At embryonic day 4, blastocysts of the d-hES cell group were transferred into pseudo-pregnant recipients, and pregnancy rate (75.0%) was very high compared to the other groups (M16, 57.1%; CR1aa, 37.5%). In addition, embryo implantation (55.9%) and live fetus rate (38.2%) of the d-hES cell group were also better than those of the other groups (M16, 36.7 and 18.3%, respectively; CR1aa, 23.2 and 8.7%, respectively). These results demonstrated that d-hES cells can be used as a feeder cell for enhancing in vitro and in vivo developmental potential of mouse pre-implantation embryos.
Mouse Embryo;d-hES Feeder Cell;Co-culture;Developmental Potential;
 Cited by
Assady, S., G. Maor, M. Amit, J. Itskovitz-Eldor, K. L. Skorecki and M. Tzukerman. 2001. Insulin production by human embryonic stem cells. Diabetes 50(8):1691-1697. crossref(new window)

Bavister, B. D., T. A. Rose-Hellekant and T. Pinyopummintr. 1992. Development of in vitro matured/in vitro fertilized bovine embryos into morulae and blastocysts in defined culture media. Theriogenology 37:127-146. crossref(new window)

Fouladi-Nashta, A. A., C. J. Jones, N. Nijjar, L. Mohamet, A. Smith, I. Chambers and S. J. Kimber. 2005. Characterization of the uterine phenotype during the periimplantation period for LIF-null, MF1 strain mouse. Dev. Biol. 281(1):1-21. crossref(new window)

Fukuda, Y., M. Ichikawa, K. Naito and Y. Toyoda. 1990. Birth of normal calves resulting from bovine oocytes matured, fertilized, and cultured with cumulus cells in vitro up to the blastocyst stage. Biol. Reprod. 42:114-119. crossref(new window)

Hotoya, S., Y. Sugiyama, R. Torii, V. Wijewardana, D. Kumagai, K. Sugiura, K. Kida, N. Kawate, H. Tamada and T. Sawada. 2006. Effect of co-culturing with embryonic fibroblasts on IVM, IVF and IVC of canine oocytes. Theriogenology 1566(5):1083-1090.

Hwu, Y., R. K. Lee, C. Chen, J. Su, Y. Chen and S. Lin. 1998. Development of hatching blastocysts from immature human oocytes following in-vitro maturation and fertilization using a co-culture system. Hum. Reprod. 13:1916-1921. crossref(new window)

Kaufman, D. S., E. T. Hanson, R. L. Lewis, R. Auerbach and J. A. Thomson. 2001. Hematopoietic colony-forming cells derived from human embryonic stem cells. Proc. Natl. Acad. Sci. USA. 98(19):10716-10721. crossref(new window)

Kehat, I., D. Kenyagin-Karsenti, M. Snir, H. Segev, M. Amit, A. Gepstein, E. Livne, O. Binah, J. Itskovitz-Eldor and L. Gepstein. 2001. Human embryonic stem cells can differentiate into myocytes with structural and functional properties of cardiomyocytes. J. Clin. Invest. 108(3):407-414. crossref(new window)

Khatir, H., A. Anouassi and A. Tibary. 2004. Production of dromedary (Camelus dromedaries) embryos by IVM and IVF and co-culture with oviductal or granulosa cells. Theriogenology 62(7):1175-1185. crossref(new window)

Malekshah, A. K. and A. E. Moghaddam. 2005. Follicular fluid and cumulus cells synergistically improve mouse early embryo development in vitro. J. Reprod. Dev. 51(2):195-199. crossref(new window)

Mercader, A., J. A. Garcia-Valesco, E. Escudero, J. Remohi, A. Pellicer and C. Simon. 2003. Clinical experience and perinatal outcome of blastocyst transfer after co-culture of human embryos with human endometrial epithelial cells: a 5-year follow-up study. Fertil. Steril. 80(5):1162-1168. crossref(new window)

Noh, J. H., K. N. Chung and Y. B. Kim. 2006. The effect of Vero cell co-culture on the development of mouse embryos exposed to monoclonal antibodies specific for mammalian heat shock protein 60. J. Korean Med. Sci. 21(2): 304-308. crossref(new window)

Park, S. P., Y. J. Lee, K. S. Lee, H. A. Shin, H. Y. Cho, K. S. Chung, E. Y. Kim and J. H. Lim. 2004. Establishment of human embryonic stem cell lines from frozen-thawed blastocysts using STO cell feeder layers. Hum. Reprod. 19(3):676-684. crossref(new window)

Reubinoff, B. E., M. F. Pera, C. Y. Fong, A. Trounson and A. Bongso. 2000. Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro. Nat. Biotechnol. 18:399-404. crossref(new window)

Rohwedel, J., K. Guan and A. M. Wobus. 1999. Induction of cellular differentiation by retinoic acid in vitro. Cells Tissues Organs 65:190-202.

Schuldiner, M., O. Yanuka, J. Itskovitz-Eldor, D. A. Melton and N. Benvenisty. 2000. Effects of eight growth factors on the differentiation of cells derived from human embryonic stem cells. Proc. Natl. Acad. Sci. USA 97(21):11307-11312. crossref(new window)

Smith, A. G., J. K. Heath, D. D. Donaldson, G. G. Wong, J. Moreau, M. Stahl and D. Rogers. 1988. Inhibition of pluripotential embryonic stem cell differentiation by purified polypeptides. Nature 359:693-699.

Srinivasan, K. R., A. Dwivedi, S. K. Jain and P. K. Mehrotra. 2006. Demonstration of uterine receptivity in vitro by co-culture of rat epithelial cells and blastocyst. Cell Tissue Res. 325(1):135-141. crossref(new window)

Thomson, J. A., J. Itskovitz-Eldor, S. S. Shapiro, M. A. Waknitz, J. J. Swiergiel, V. S. Marshall and J. M. Jones. 1998. Embryonic stem cell lines derived from human blastocysts. Science 282(5391):1145-1147 crossref(new window)

Tomida, M., Y. Yamamoto-Yamaguchi and M. Hozumi. 1984. Purification of a factor inducing differentiation of mouse myeloid leukemic M1 cells from conditioned medium of mouse fibroblast L929 cells. J. Biol. Chem. 259:10978-10982.

Yang, Z. M., S. P. Le, D. B. Chen, J. Cota, V. Siero, K. Yasukawa and M. J. Harper. 1995. Leukemia Inhibitory factor, LIF receptor, and gp130 in the mouse uterus during early pregnancy. Mol. Reprod. Dev. 42(4):407-414. crossref(new window)