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Detection of the SRY Transcript and Protein in Bovine Ejaculated Spermatozoa
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 Title & Authors
Detection of the SRY Transcript and Protein in Bovine Ejaculated Spermatozoa
Li, Chunjin; Sun, Yongfeng; Yi, Kangle; Li, Chengjiao; Zhu, Xiaoling; Chen, Lu; Zhou, Xu;
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The sex-determining region on the Y (SRY) gene is important in mammalian sex determination and differentiation. We report a study of the abundance of SRY gene products in bovine ejaculate. RT-PCR experiments using RNA extracted from bovine spermatozoa with SRY-specific primers yielded a 456 bp product, but the amount of SRY mRNA in sperm was lower than that in the testes (p<0.01). A protein of approximately 27 KDa was detected by western blotting. The SRY transcript was detected in the midpiece of approximately half the spermatozoa by in situ hybridization, and the SRY protein was detected in the heads of half the spermatozoa by immunofluorescence, indicating that SRY mRNA and protein may only be present in Y-bearing spermatozoa. These results suggest that the SRY transcript and protein are present in bovine ejaculated Y-sperm. The roles of the SRY gene in spermatogenesis, sperm motility, and the sperm-oocyte interaction merit further investigation.
SRY Gene;Bovine Spermatozoa;In situ Hybridization;Bovine;
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Aquila, S., M. Gentile, E. Middea, S. Catalano and S. Ando. 2004. Autocrine regulation of insulin secretion in human ejaculated spermatozoa. Endocrinology 146(2):552-557. crossref(new window)

Aquila, S., M. Gentile, E. Middea, S. Catalano, C. Morelli, V. Pezzi and S. Ando. 2005. Leptin Secretion by Human Ejaculated Spermatozoa. J. Clin. Endocrinol. Metab. 90(8):4753-4761. crossref(new window)

Dadoune, J. P., A. Pawlak, M. F. Alfonsi and J. P. Siffroi. 2005. Identification of transcripts by macroarrays, RT-PCR and in situ hybridization in human ejaculate spermatozoa. Mol. Hum. Reprod. 11(2):133-140. crossref(new window)

Dadoune, J. P. 2003. Expression of mammalian spermatozoal nucleoproteins. Microsc. Res. Tech. 61(1):56-75. crossref(new window)

Gilbert, I., N. Bissonnette, G. Bossinneault, M. Valee and C. Robert. 2007. A molecilar analysis of the population of mRNA in bovine spermatozoa. Reproduction 133(6):1073-1086. crossref(new window)

Giordano, R., A. R. Magnano, G. Zaccagnini, C. Pittoggi, N. Moscufo, R. Lorenzini and C. Spadafora. 2000. Reverse transcriptase activity in mature spermatozoa of mouse. J. Cell. Biol. 148(6):1107-1113. crossref(new window)

Goodfellow, P. N. and R. Lovell-Badge. 1993. SRY and sex determination in mammals. Annu. Rev. Genet. 27:71-92. crossref(new window)

Goodwin, L. O., D. S. Karabinus, R. G. Pergolizzi and S. Benoff. 2000. L-type voltage dependent calcium channel alpha-1C subunit mRNA is present in ejaculated human spermatozoa. Mol. Hum. Reprod. 6(2):127-136. crossref(new window)

Goodwin, L. O., D. S. Karabinus and R. G. Pergolizzi. 2000. Presence of N-cadherin transcripts in mature spermatozoa. Mol. Hum. Reprod. 6(6):487-497. crossref(new window)

Gur, Y. and H. Breitbart. 2006. Mammalian sperm translate nuclear-encoded proteins by mitochondrial-type ribosomes. Genes Dev. 20(4):411-416. crossref(new window)

Hacker, A., B. Capel, P. N. Goodfellow and R. Lovell-Badge. 1995. Expression of SRY, the mouse sex determining gene. Development 121(6):1603-1614.

Hanley, N. A., D. M. Hagan, M. Clement-Jones, S. G. Ball, T. Strachan, L. Salsa-Cortes, K. McElreavey, S. Lindsay, S. Robson, P. Bullen, H. Ostrer and D. I. Wilson. 2000. SRY, SOX9 and DAX1 expression patterns during human sex determination and gonadal development. Mech. Dev. 91(1-2):403-407. crossref(new window)

Koopman, P., J. Gubbay, N. Vivian, P. Goodfellow and R. Lovell-Badge. 1991. Male development of chromosomally female mice transgenic for Sry. Nature 351(6322):117-121. crossref(new window)

Lambard, S., I. Galeraud-Denis, G. Martin, R. Levy, A. Chocat and S. Carreau. 2004. Analysis and significance of mRNA in human ejaculated sperm from normozoospermic donors: relationship to sperm motility and capacitation. Mol. Hum. Reprod. 10(7):535-541. crossref(new window)

Lambard, S., I. Galeraud-Denis, P. T. Saunders and S. Carreau. 2004. Human immature germ cells and ejaculated spermatozoa contain aromatase and oestrogen receptors. J. Mol. Endocrinol. 32(1):279-289. crossref(new window)

Li, C. J., C. J. Li, X. L. Zhun, C. Q. Wang, Z. Liu, W. H. Li and C. Lu. The expression and putative role of brain-derived neurotrophic factor and its receptor in bovine sperm. (unpublished)

Lin, Y. M., C. W. Chen, H. S. Sun, S. J. Tsai, J. S. Nan and P. L. Kuo. 2002. Presence of DAZL transcript and protein in mature human spermatozoa. Fertil. Steril. 77(3):626-629. crossref(new window)

Modi, D., P. Berde and D. Bhartiya. 2003. Down syndrome: a study of chromosomal mosaicism. Reprod. Biomed. Online. 6(4):499-503. crossref(new window)

Modi, D., S. Sane and D. Bhartiya. 2003. Accelerated germ cell apoptosis in sex chromosome aneuploid fetal human gonads. Mol. Hum. Reprod. 9(4):219-225. crossref(new window)

Modi, D., C. Shah, G. Sachdeva, S. Gadka, D. Bhartiya and C. Puri. 2005. Ontogeny and cellular location of SRY transcripts in the human testes and its detection in spermatozoa. Reproduction 130(5):603-613. crossref(new window)

Ostermeier, G. C., D. J. Dix, D. Miller, P. Khatri and S. A. Krawetz. 2002. Spermatozoal RNA profiles of normal fertile men. Lancet 360(9335):772-777. crossref(new window)

Ostermeier, G. C., D. Miller, J. D. Huntriss, M. P. Diamond and S. A. Krawetz. 2004. Reproductive biology: delivering spermatozoan RNA to the oocyte. Nature 429(6988):154.

Parrish, J. J., J. L. Susko-Parrish, M. L. Leibfried-Rutledge, E. S. Critser, W. H. Eyestone and N. L. First. 1986. Bovine in vitro fertilization with frozen-thawed semen. Theriogenology 25(4):591-600. crossref(new window)

Parsons, R. 2004. Human cancer, PTEN and the PI-3 kinase pathway. Semin. Cell Dev. Biol. 15(2):171-176. crossref(new window)

Shah, C. A., D. Modi, G. Sachdeva, S. Gadkar, S. D'Souza and C. P. Puri. 2005. N terminal region of progesterone receptor B isoform in human spermatozoa. Int. J. Androl. 28(6):360-371. crossref(new window)

Steger, K. 2003. Perspectives in the diagnosis of testicular biopsies using molecular biological techniques. Andrologia 35(3):183.

Zuccarello, D., A. Garolla, A. Ferlin, M. Menegazzo, L. D. Toni, M. Carraro, C. Veronese and C. Foresta. 2009. Androgen receptor is expressed in both X- and Y-carrier human spermatozoa. Fertil. Steril. 91(1):193-200. crossref(new window)