JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Genetic Diversity of Chinese Indigenous Pig Breeds in Shandong Province Using Microsatellite Markers
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Genetic Diversity of Chinese Indigenous Pig Breeds in Shandong Province Using Microsatellite Markers
Wang, J.Y.; Guo, J.F.; Zhang, Q.; Hu, H.M.; Lin, H.C.; Wang, Cheng; Zhang, Yin; Wu, Y.;
  PDF(new window)
 Abstract
To investigate the genetic diversity of six Chinese indigenous pig breeds in Shandong province (Laiwu Black, Dapulian Black, Licha Black, Yantai Black, Yimeng Black and Wulian Black), explain their genetic relationship and assess their integrity and degree of admixture with three Western commercial breeds (Landrace, Yorkshire and Duroc), 303 individuals from these breeds were genotyped for 26 microsatellite markers. In general, high genetic diversity (observed heterozygosity ranging from 0.5495 to 0.7746) and large breed differentiation ( = 0.188) were observed. The indigenous pig breeds in Shandong exhibited consistently higher levels of genetic diversity than the three Western breeds. However, compared with the Western breeds, which have an value of 0.252, the indigenous breeds in Shandong have smaller value of 0.145. The analysis of breed relationship indicated that the six indigenous breeds are classified into two groups. One includes four breeds, Licha, Yantai, Yimeng and Wulian, which have experienced large gene introgression of the Western breeds through progressive crossbreeding as well as gene flow among themselves. The other includes Laiwu and Dapulian, which are less influenced by the Western breeds and other indigenous breeds in Shandong in the recent past. The results show that some measures must be taken to effectively protect these indigenous pig breeds in Shandong.
 Keywords
Microsatellite Marker;Genetic Diversity;Chinese Indigenous Pig Breeds;Shandong Province;
 Language
English
 Cited by
1.
Molecular genetic diversity and genetic structure of Vietnamese indigenous pig populations, Journal of Animal Breeding and Genetics, 2014, 131, 5, 379  crossref(new windwow)
 References
1.
Chang, W. H., H. P. Chu, Y. N. Jiang, S. H. Li, Y. Wang, C. H. Chen, K. J. Chen, C. Y. Lin and Y. T. Ju. 2009. Genetic variation and phylogenetics of Lanyu and exotic pig breeds in Taiwan analyzed by nineteen microsatellite markers. J. Anim. Sci. 87(1):1-8. crossref(new window)

2.
Fan, B., Z. G. Wang, Y. J. Li, X. L. Zhao, B. Liu, S. H. Zhao, M. Yu, M. H. Li, S. L. Chen, T. A. Xiong and K. Li. 2002. Genetic variation analysis within and among Chinese indigenous swine populations using microsatellite markers. Anim. Genet. 33(6):422-427. crossref(new window)

3.
Fang, M. and L. Andersson. 2006. Mitochondrial diversity in European and Chinese pigs is consistent with population expansions that occurred prior to domestication. Proc. R. Soc. B. 273(1595):1803-1810. crossref(new window)

4.
Fang, M., X. Hu, T. Jiang, M. Braunschweig, L. Hu, Z. Du, J. Feng, Q. Zhang, C. Wu and N. Li. 2005. The phylogeny of Chinese indigenous pig breeds inferred from microsatellite markers. Anim. Genet. 36(1):7-13. crossref(new window)

5.
Felsenstein, J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39:783-791. crossref(new window)

6.
Goudet, J. 2001. Fstat, a program to estimate and test gene diversities and fixation indices (version 2.9.3). Available from http://www.Unil.Ch/izea/softwares/fstat.Html.

7.
Guo, S. W. and E. A. Thompson. 1992. Performing the exact test of Hardy-Weinberg proportion for multiple alleles. Biometrics 48(2):361-372. crossref(new window)

8.
Jiang, Y. N., C. Y. Wu, C. Y. Huang, H. P. Chu, M. W. Ke, M. S. Kung, K. Y. Li, C. H. Wang, S. H. Li, Y. Wang and Y. T. Ju. 2008. Interpopulation and intrapopulation maternal lineage genetics of Lanyu pig (Sus scrofa) by analysis of mitochondrial cytochrome b and control region sequences. J. Anim. Sci. 86:2461-2470. crossref(new window)

9.
Li, S. J., S. L. Yang, S. H. Zhao, B. Fan, M. Yu, H. S. Wang, M. H. Li, B. Liu, T. A. Xiong and K. Li. 2004. Genetic diversity analyses of 10 indigenous Chinese pig populations based on 20 microsatellites. J. Anim. Sci. 82(2):368-374.

10.
Megens, H. J., R. P. Crooijmans, M. San Cristobal, X. Hui, N. Li and M. A. Groenen. 2008. Biodiversity of pig breeds from China and Europe estimated from pooled DNA samples: Differences in microsatellite variation between two areas of domestication. Genet. Sel. Evol. 40(1):103-128.

11.
Nei, M. 1972. Genetic distance between populations. Am. Nat. 106:283-292. crossref(new window)

12.
Ota, T. 1993. Dispan: Genetic distance and phylogenetic analysis. Pennsylvania State University, USA.

13.
Park, S. D. E. 2001. Trypanotolerance in West African cattle and the population genetic effects of selection. [ph.D.Thesis], University of Dublin.

14.
Pritchard, J. K., M. Stephens and P. Donnelly. 2000. Inference of population structure using multilocus genotype data. Genetics 155:945-959.

15.
Rousset, F. 2008. GENEPOP'007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol. Ecol. Resour. 8(1):103-106. crossref(new window)

16.
Saitou, N. and M. Nei. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4(4):406-425.

17.
Sambrook, J. and D. Russell. 2001. Molecular cloning: A laboratory manual. 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA.

18.
Si, J. C., H. B. Zhang and J. J. Tang. 1999. Livestock breeds in Shandong province. Haitian Press, Shenzhen, China.

19.
Sneath, P. H. A. and R. R. Sokal. 1973. Numerical taxonomy. W. H. Freeman, San Francisco.

20.
Wang, J. Y., J. F. Guo, S. L. Sun, C. Wang, Y. Zhang and Y. Wu. 2009. The genetic diversity and phylogenetic relationship among pig breeds of Shandong province based on partial sequence of mtDNA D-Loop region. Chinese J. Anim. Vet. Sci. 40(6):792-799.

21.
Wang, J. Y., J. F. Guo, X. J. Hao, H. M. Hu, H. C. Lin, Y. Zhang, C. Wang and Y. Wu. 2010. Phylogenetic relationship of pig breeds from Shandong province of China and their influence by modern commercial breeds by analysis of mitochrondrial DNA sequences. Ital. J. Anim. Sci. 9:e48.

22.
Weir, B. S. and C. C. Cockerham. 1984. Estimating F-statistics for the analysis of population structure. Evolution. 38(6):1358-1370.

23.
Wu, G. S., Y. G. Yao, K. X. Qu, Z. L. Ding, H. Li, M. G. Palanichamy, Z. Y. Duan, N. Li, Y. S. Chen and Y. P. Zhang. 2007. Population phylogenomic analysis of mitochondrial DNA in wild boars and domestic pigs revealed multiple domestication events in eastern Asia. Genome Biol. 8(11):R245. crossref(new window)

24.
Yang, J., J. Wang, J. Kijas, B. Liu, H. Han, M. Yu, H. Yang, S. Zhao and K. Li. 2003a. Genetic diversity present within the near-complete mtDNA genome of 17 breeds of indigenous Chinese pigs. J. Hered. 94(5):381-385. crossref(new window)

25.
Yang, S. L., Z. G. Wang, B. Liu, G. X. Zhang, S. H. Zhao, M. Yu, B. Fan, M. H. Li, T. A. Xiong and K. Li. 2003b. Genetic variation and relationships of eighteen Chinese indigenous pig breeds. Genet. Sel. Evol. 35(6):657-671. crossref(new window)

26.
Zhang, G. X., Z. G. Wang, F. Z. Sun, W. S. Chen, G. Y. Yang, S. J. Guo, Y. J. Li, X. L. Zhao, Y. Zhang, J. Sun, B. Fan, S. L. Yang and K. Li. 2003. Genetic diversity of microsatellite loci in fifty-six Chinese native pig breeds. Yi Chuan Xue Bao. 30(3):225-233.

27.
Zhang Z. G., B. D. Li and X. H. Chen. 1986. Pig breeds in China. Shanghai Scientific and Technical Publisher, Shanghai, China.