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Isolation and Characterization of Parthenogenetic Embryonic Stem (pES) Cells Containing Genetic Background of the Kunming Mouse Strain
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 Title & Authors
Isolation and Characterization of Parthenogenetic Embryonic Stem (pES) Cells Containing Genetic Background of the Kunming Mouse Strain
Yu, Shu-Min; Yan, Xing-Rong; Chen, Dong-Mei; Cheng, Xiang; Dou, Zhong-Ying;
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 Abstract
Parthenogenetic embryonic stem (pES) cells could provide a valuable model for research into genomic imprinting and X-linked diseases. In this study, pES cell lines were established from oocytes of hybrid offspring of Kunming and 129/Sv mice, and pluripotency of pES cells was evaluated. The pES cells maintained in the undifferentiated state for more than 50 passages had normal karyotypes with XX sex chromosomes and exhibited high activities of alkaline phosphatase (AKP) and telomerase. Meanwhile, these cells expressed ES cell molecular markers SSEA-1, Oct-4, Nanog, and GDF3 but not SSEA-3 detected by immunohistochemistry and RT-PCR. The pES cells could be differentiated into various types of cells from three germ layers in vitro by analysis of embryoid bodies (EBs) with immunohistochemistry and RT-PCR, and in vivo by observation of pES cell-derived teratoma sections. Therefore, the established pES cell lines contained all features of mouse ES cells. This work provides a new strategy for isolating pES cells from Kunming mice, and the pES cell lines could be applied as the cell model in research into genomic imprinting and epigenetic regulation of Kunming mice.
 Keywords
Mouse;Parthenogenetical Activation;Parthenogenetic Embryonic Stem (pES) Cells;Kunming Mouse (Mus musculus Km);Genetic Background;
 Language
English
 Cited by
1.
Improved Establishment of Embryonic Stem (ES) Cell Lines from the Chinese Kunming Mice by Hybridization with 129 Mice, International Journal of Molecular Sciences, 2014, 15, 3, 3389  crossref(new windwow)
2.
Generation of parthenogenetic goat blastocysts: effects of different activation methods and culture media, Zygote, 2015, 23, 03, 327  crossref(new windwow)
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