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Relationships of Plasma and Very Low Density Lipoprotein Lipids and Subfractions with Abdominal Fat in Chickens
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 Title & Authors
Relationships of Plasma and Very Low Density Lipoprotein Lipids and Subfractions with Abdominal Fat in Chickens
Loh, Teck Chwen; Tan, Bee Koon; Foo, Hooi Ling; Norhani, Abdullah; Zulkifli, Idrus;
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A study was conducted to determine the relationships between triacylglycerol (TAG) of plasma, very low density lipoprotein (VLDL) and fat deposition in two different breeds of chickens. The VLDL apolipoproteins of both breeds were also characterised. The breeds used were crossbred village chicken (AK) (Sasso crossed) and commercial broiler (CB) (Avian). They were housed in six pens with 30 female and 30 male birds of each breed per pen. Three male and three female birds from each pen were slaughtered and the blood was collected. The VLDL was isolated and sub-fractionated using Fast Protein Liquid Chromatography (FPLC). VLDL TAG of CB was significantly lower than AK. The particle size was negatively correlated with VLDL TAG and positively correlated with abdominal fat. Sub-fraction 2 contained more apo E that will enhance the lipolysis process of the VLDL TAG than subfraction 1. CB had a higher proportion of sub-fraction 2 than AK. The results showed that the proportion of sub-fraction 2 was negatively correlated with VLDL TAG concentration and positively correlated with abdominal fat.
Very Low Density Lipoprotein;Broiler;Crossbred Village Chicken;Apolipoprotein;
 Cited by
Bachorik, P. S. 1982. Collection of blood samples for lipoprotein analysis. Clin. Chem. 28/6:1375-1378.

Benson, J. D., V. Hearn, T. Boyd and A. Bensadoun. 1975. Triacylglycerol hydrolase of chicken and rat pre- and postheparin plasma. Effects of fasting and comparison with adipose tissue lipoprotein lipase. Int. J. Biochem. 6:727-734. crossref(new window)

Clark, A. B. and S. H. Quarfordt. 1985. Apolipoprotein effects on the lipolysis of perfused triglyceride by heparinimmobilised milk lipase. J. Biol. Chem. 260:4778-4783.

Cryer, A. 1981. Tissue lipoprotein lipase activity and its action in lipoprotein metabolism. Int. J. Biochem. 13: 525-541. crossref(new window)

Eisenberg, S. and T. Olivecrona. 1979. Very low density lipoprotein. Fate of phospholipid, cholesterol and apolipoprotein C during lipolysis in vitro. J. Lipid Res. 20:614-623.

Elovson, J., J. E. Chatterton, G. T. Bell, V. N. Schumaker, M. A. Reuben, D. L. Puppione, J. R. Reeve, Jr. and N. L. Young. 1988. Plasma very low density lipoprotein contain a single molecule of apolipoprotein B. J. Lipid Res. 29:1461-1473.

Evans, A. J., M. W. Huff and B. M. Wolfe. 1989. Accumulation of an apo-E poor subfraction of very low density lipoprotein in hypertriglyceridemic men. J. Lipid Res. 30:1691-1701.

Fungwee, T. V., L. Cagen, H. G. Wilcox and M. Heimberg. 1992. Regulation of hepatic secretion of very low density lipoprotein by dietary cholesterol. J. Lipid Res. 33:179-191.

Gaya, L. G., G. B. Mourão, E. M. de Rezende, E. C. de Mattos, T. M. Filho, L. G. Figueiredo, J. B. Ferraz and J. P. Eler. 2005. Genetic trends of abdominal fat content in a male broiler chicken line. Genet. Mol. Res. 4:760-764.

Gibson, J. C. and W. V. Brown. 1988. Effect of lipoprotein lipase and hepatic triacylglycerol lipase activity on the distribution of apolipoprotein E among the plasma lipoprotein. Atherosclerosis 73:45-55. crossref(new window)

Gomez-Coronado, D., G. T. Saez, M. A. Lasuncion and E. Herrera. 1993. Different hydrolytic efficiencies of adipose tissue lipoprotein lipase on very low density lipoprotein subfractions separated by heparin-Sepharose chromatography. Biochim. Biophys. Acta 1167:70-78. crossref(new window)

Gornall, A. G., C. S. Bardawill and M. M. David. 1949. Determination of serum protein by means of Biuret reaction. J. Biol. Chem. 177:751.

Griffin, H. D., K. Guo, D. Windsor and S. C. Butterwith. 1992. Adipose tissue lipogenesis and fat deposition in leaner broiler chickens. J. Nutr. 122:363-368.

Griffin, H. D., D. Windsor and C. C. Whitehead. 1991. Changes in lipoprotein metabolism and body composition in chickens in response to divergent selection for plasma very low density lipoprotein concentration. Br. Poult. Sci. 32:195-201. crossref(new window)

Hermier, D. 1997. Lipoprotein metabolism and fattening in poultry. J. Nutr. 127:805S-808S.

Huff, M. W. and D. E. Telford. 1984. Characterisation and metabolic fate of two very low density lipoprotein subfractions separated by heparin-Sepharose chromatography. Biochim. et Biophys. Acta 796:251-261. crossref(new window)

Loh, T. C., I. J. Lean and P. E. Dodds. 2001. Association of backfat thickness with postheparin lipoprotein lipase activity and very low density lipoprotein subfractions. Asian-Aust. J. Anim. Sci. 14:1592-1597. crossref(new window)

Lowry, O. H., N. J. Rosebrough, A. L. Farr and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265-275.

Maguire, G. F., M. Lee and P. W. Connelly. 1989. Sodium dodecyl sulfate-glycerol polyacrylamide slab gel electrophoresis for the resolution of apolipoproteins. J. Lipid Res. 30:757-761.

Minitab. 1995. Minitab reference manual release 10. Pennsylvania, Pennsylvania State College.

Nestel, P., T. Billington, N. Tada, P. Nugent and N. Fidge. 1983. Heterogeneity of very low density lipoprotein metabolism in hyperlipidemic subjects. Metabolism 32:810-817. crossref(new window)

Nestel, P. J., W. E. Coonor, M. F. Reardon, S. Connor, S. Wong and R. Boston. 1984. Suppression by diets rich in fish oil of very low density lipoprotein production in man. J. Clin. Invest. 74:82-89. crossref(new window)

Nozaki, S., M. Kubo, Y. Matsuzawa and S. Tarui. 1984. Sensitive non-radioisotopic method for measuring lipoprotein lipase and hepatic triglyceride lipase in postheparin plasma. Clin. Chem. 30/5:748-751.

Saadoun, A. and B. Leclercq. 1987. In vivo lipogenesis of genetically lean and fat chickens: effects of nutritional state and dietary fat. J. Nutr. 117:428-435.

SAS. 1989. Statistical analysis systems. SAS/STAS User's Guide. Release 6.08 edition. SAS Institute INC., Cary. NC.

Smith, L. C., H. J. Pownall and A. M. Gotto. 1978. The plasma lipoproteins: structure and metabolism. Ann. Rev. Biochem. 47:751-777. crossref(new window)

Tan, B. K., H. L. Foo, T. C. Loh, A. Norhani and I. Zulkifli. 2005. Purification and characterisation of very low density lipoprotein in commercial broiler and crossbred village chickens by fast liquid chromatography. Asian-Aust. J. Anim. Sci. 18:1780-1785. crossref(new window)

Tam, S. P., L. Dory and D. Rubinstein. 1981. Fate of apolipoproteins CII, CIII and E during lipolysis of human very low density lipoprotein in vitro. J. Lipid Res. 222:641-651.

Trezzi, E., C. Calvi, P. Roma and A. L. Catapano. 1983. Subfractionation of human very low density lipoprotein by heparin-Sepharose chromatography. J. Lipid Res. 24:790-795.

Whitehead, C. C. 1988. Selection for leanness in broilers using plasma lipoprotein concentration as selection criterion. In Leanness in domestic birds. pp 41-57. London, UK. Butterworth & Co.

Whitehead, C. C. and H. D. Griffin. 1982. Plasma lipoprotein concentration as an indicator of fatness in broilers: effect of age and diet. Br. Poult. Sci. 23:299-305. crossref(new window)

Wright, M. 1993. Metabolism of very low density lipoprotein of sow. PhD Thesis, Wye College, University of London.

Wright, M., I. J. Lean, E. Herrera and P. F. Dodds. 1995. Changes in the composition of plasma very low density lipoprotein during pregnancy and lactation in genetic lines of pigs. Anim. Sci. 61:361-365. crossref(new window)

Young, S. G. 1990. Recent progress in understanding apolipoprotein B. Circulation 82/5:1574-1594. crossref(new window)

Zubair, A. K. and S. Leeson. 1996. Compensatory growth in the broiler chicken : a review. World's Poult. Sci. J. 52:189-201. crossref(new window)