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Ileal Digestibility of Amino Acids in Conventional and Low-Kunitz Soybean Products Fed to Weanling Pigs
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 Title & Authors
Ileal Digestibility of Amino Acids in Conventional and Low-Kunitz Soybean Products Fed to Weanling Pigs
Goebel, K.P.; Stein, H.H.;
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An experiment was conducted to determine the standardized ileal digestibility (SID) of amino acids (AA) in four sources of full-fat soybeans (FFSB) and in one source of soybean meal (SBM). The FFSB had different concentrations of trypsin inhibitor units (TIU) and included two sources of conventional FFSB, and two sources of a soybean variety that was selected for a reduced concentration of the Kunitz trypsin inhibitor. The conventional FFSB was either low temperature-processed (LT-FFSB-CV; 37.7% CP, 35.4 TIU/mg) or high temperature-processed (HT-FFSB-CV; 40.5% CP, 4.4 TIU/mg). The low-Kunitz FFSB was also either low temperature-processed (LT-FFSB-LK; 36.2% CP, 23.5 TIU/mg) or high temperature-processed HT-FFSB-LK; (38.2% CP, 4.0 TIU/mg). The SBM contained 47.5% CP and 3.20 TIU/mg. Twelve weanling barrows (initial BW: ) were fitted with a T-cannula in the distal ileum. Pigs were allotted to a replicated Latin square design with six diets and six periods per square. Five diets were prepared using each of the soybean sources as the only source of AA in the diet. An N-free diet was also included in the experiment to measure basal endogenous losses of AA. The two low temperature-processed FFSB had lower (p<0.05) AID and SID values for all indispensable AA than the two high temperature-processed FFSB and SBM. The SID values for all indispensible AA except Trp were greater (p<0.05) in LT-FFSB-LK than in LT-FFSB-CV, but the SID of AA in HT-FFSB-CV and HT-FFSB-LK were not different. The SID of AA in SBM were not different from the SID in HT-FFSB-CV and in HT-FFSB-LK. Results of this experiment show that a reduction of the TIU from 35.4 to 23.5 TIU/mg will improve the SID of AA, but this reduction is not sufficient to completely ameliorate the negative impact of trypsin inhibitors. Results also show that the SID of AA in high temperature-processed FFSB is similar to that in de-hulled SBM.
Amino Acid Digestibility;Low Kunitz Soybeans;Full-fat Soybean;Trypsin Inhibitor;Pig;
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AOAC Int. 2007. Official methods of analysis .18th ed. Rev. 2 (Ed. W. Howitz, and G. W. Latimer Jr.). AOAC Int., Gaithersburg, MD.

AOCS. 2006. Official methods and recommended practices (5th Ed). Association of Oil Chemists Society. Urbana, IL.

Cervantes-Pahm, S. K. and H. H. Stein. 2008. Effect of dietary soybean oil and soybean protein concentration on the concentration of digestible amino acids in soybean products fed to growing pigs. J. Anim. Sci. 86:1841-1849. crossref(new window)

Clark, R. W. and T. Hymowitz. 1972. Activity variation between and within two soybean trypsin inhibitor eletrophoretic forms. Biochem. Genet. 6:169-182. crossref(new window)

Clemente, A., E. Jimenez, M. C. Marin-Manzano and L. A. Rubio. 2008. Active Bowman-Birk inhibitors survive gastrointestinal digestion at the terminal ileum of pigs fed chickpea-based diets. J. Sci. Food Agric. 88:513-521. crossref(new window)

Cook, D. A., A. H. Jensen, J. R. Fraley and T. Hymowitz. 1988. Utilization by growing and finishing pigs of raw soybeans of low Kunitz trypsin inhibitor content. J. Anim. Sci. 66:1686-1691.

Dilger, R. N., J. S. Sands, D. Ragland and O. Adeola. 2004. Digestibility of nitrogen and amino acids in soybean meal with added soyhulls. J. Anim. Sci. 82:715-724.

Gentilicore, D., R. Chaikomin, K. L. Jones, A. Rusco, C. Feinle-Bisset, J. M. Wishart, C. K. Rayner and M. Horitz. 2006. Effect of fat on gastric emptying and the glycemic, insulin, and incretin responses to a carbohydrate meal and Type 2 diabetes. J. Clin. Endocrinol. Metab. 91:2062-2067. crossref(new window)

Herkelman, K. L., G. L. Cromwell, T. S. Stahly, T. W. Pfeiffer and D. A. Knabe. 1992. Apparent digestibility of amino acids in raw and heated conventional and low-trypsin-inhibitor soybeans for pigs. J. Anim. Sci. 70:818-826.

Kim, B. G. and H. H. Stein. 2009. A spreadsheet program for making a balanced latin square design. Rev. Colomb. Cienc. Pecu. 22:591-596.

Kim, I. H., J. D. Hancock, D. B. Jones and P. G. Reddy. 1999. Extrusion processing of low-inhibitor soybeans improves growth performance of early-weaned pigs. Asian-Aust. J. Anim. Sci. 12:1251-1257. crossref(new window)

Kunitz, M. 1947a. Crystalline soybean trypsin inhibitor. J. Gen. Physiol. 30:291-310. crossref(new window)

Kunitz, M. 1947b. Isolation of a crystalline protein compound of trypsin and of soybean trypsin-inhibitor. J. Gen. Physiol. 30:311-320. crossref(new window)

Liener, I. E. 1981. Factors affecting the nutritional quality of soya products. J. Am. Oil Chem. Soc. 58:406-415. crossref(new window)

Liener, I. E. and M. L. Kakade. 1980. Protease inhibitors. In: Toxic Constituents of plant foodstuffs (Ed. I. E. Liener). pp 7-71. Academic Press, New York.

Losso, J. N. 2008. The biochemical and functional food properties of the Bowman-Birk inhibitor. Crit. Rev. Food Sci. Nutr. 48:94-118. crossref(new window)

NRC. 1998. Nutrient requirements of swine (10th Ed.). National Academy Press, Washington DC.

Palacios, M. F., R. A. Easter, K. T. Soltwedel, C. M. Parsons, M. W. Douglas, T. Hymowitz and J. E. Pettigrew. 2004. Effect of soybean variety and processing on growth performance of young chicks and pigs. J. Anim. Sci. 82:1108-1114.

Qin, G., E. R. ter Elst, M. W. Bosch and A. F. B. van der Poel. 1996. Thermal processing of whole soya beans: Studies on the inactivation of antinutritional factors and effects on ileal digestibility in piglets. Anim. Feed Sci. Technol. 57:313-324. crossref(new window)

Rackis, J. J. 1972. Biologically active components. Pages 158-202. In: Soybeans: Chemistry and Technology (Ed. A. K. Smith and S. J. Circle). Vol. I. Proteins. The AVI Publ. Co. Inc., Westport, CT.

Saxton, A. M. 1998. A macro for converting mean separation output to letter groupings in Proc Mixed. Pages 1243-1246 in Proc. 23rd SAS Users Group Intl., SAS Institute Inc., Cary, NC.

Stein, H. H., B. Sève, M. F. Fuller, P. J. Moughan and C. F. M. de Lange. 2007. Invited review: Amino acid bioavailability and digestibility in pig feed ingredients: Terminology and application. J. Anim. Sci. 85:172-180. crossref(new window)

Stein, H. H., C. F. Shipley and R. A. Easter. 1998. Technical Note: A technique for inserting a T-cannula into the distal ileum of pregnant sows. J. Anim. Sci. 76:1433-1436.

Webster, M. J., R. D. Goodband, M. D. Tokach, J. L. Nelssen, S. S. Dritz, J. C. Woodworth, M. De La Llata and N. W. Said. 2003. Evaluating processing temperature and feeding value of high temperature-processed-expelled soybean meal on nursery and finishing pig growth performance. J. Anim. Sci. 81:2032-2040.

Yen, J. T., T. Hymowitz and A. H. Jensen. 1974. Effects of soybeans of different trypsin-inhibitor activities on performance of growing swine. J. Anim. Sci. 38:304-309.

Yen, J. T., A. H. Jensen and J. Simon. 1977. Effect of dietary raw soybean and soybean trypsin inhibitor on trypsin and chymotrypsin activities in the pancreas and in small intestinal juice of growing swine. J. Nutr. 107:156-165.