Advanced SearchSearch Tips
Effects of Homolactic Bacterial Inoculant Alone or Combined with an Anionic Surfactant on Fermentation, Aerobic Stability and In situ Ruminal Degradability of Barley Silage
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effects of Homolactic Bacterial Inoculant Alone or Combined with an Anionic Surfactant on Fermentation, Aerobic Stability and In situ Ruminal Degradability of Barley Silage
Baah, J.; Addah, W.; Okine, E.K.; McAllister, T.A.;
  PDF(new window)
The effect of a homolactic inoculant containing a blend of Lactobacillus plantarum, Pediococcus acidilactici and Enterococcus faecium or, the anionic surfactant, sodium dodecyl sulphate (SDS), alone or in combination on fermentation characteristics, aerobic stability and in situ DM, OM and NDF degradability of barley silage was investigated. Barley (Hordeum vulgare, L.) was harvested (45% DM), chopped and treated with water at 24 ml/kg forage (Control), inoculant at cfu/g forage (I), SDS at 0.125% (wt/wt) of forage (S) or with the inoculant ( cfu/g) plus SDS (0.125% wt/wt; I+S). The treated forages were ensiled in triplicate mini silos and opened for chemical and microbiological analyses on d 1, 2, 3, 7, 14, 42 and 77. Silage samples from d 77 were opened and aerobically exposed for 7 d. The in situ rumen degradability characteristics of silage DM, OM and NDF were also determined. The terminal concentration of NDF in S and I+S was lower (p<0.001) than in other treatments. Lactate concentration was higher (p<0.001) and the rate and extent of pH decline were greater (p<0.001) in I and I+S than S and Control silages. A homolactic pathway of fermentation in I and I+S was evidenced by reduced (p<0.001) water-soluble carbohydrates concentration, higher lactate (p<0.01), lower acetate (p<0.01) and lower pH values (p<0.001) than in S and Control silages. All silages remained stable over 7 d of exposure to air as indicated by lower temperatures and moulds, and by non-detectable yeast populations. The treated silages had lower DM and OM degradability than in the Control but NDF degradation characteristics of I+S were improved compared to other treatments. It is concluded that the inoculant alone improved the fermentation characteristics whereas the combination of the inoculant with SDS improved both fermentation and NDF degradability of barley silage.
Aerobic Stability;Barley Silage;SDS;Homolactic Inoculant;Ruminal Degradability;
 Cited by
Effects of Microbial Additives on Chemical Composition and Fermentation Characteristics of Barley Silage,Amanullah, S.M.;Kim, D.H.;Lee, H.J.;Joo, Y.H.;Kim, S.B.;Kim, S.C.;

Asian-Australasian Journal of Animal Sciences, 2014. vol.27. 4, pp.511-517 crossref(new window)
Effects of Bacterial Inoculants and Cutting Height on Fermentation Quality of Barley Silage,Lee, Hyuk Jun;Kim, Dong Hyeon;Amanullah, Sadar M.;Kim, Sam Churl;Song, Young Min;Kim, Hoi Yun;

한국초지조사료학회지, 2014. vol.34. 3, pp.163-168 crossref(new window)
Use of thermal imaging and the in situ technique to assess the impact of an inoculant with feruloyl esterase activity on the aerobic stability and digestibility of barley silage, Canadian Journal of Animal Science, 2012, 92, 3, 381  crossref(new windwow)
Effects of Bacterial Inoculants and Cutting Height on Fermentation Quality of Barley Silage, Journal of The Korean Society of Grassland and Forage Science, 2014, 34, 3, 163  crossref(new windwow)
Effect of Microbial and Chemical Combo Additives on Nutritive Value and Fermentation Characteristic of Whole Crop Barley Silage, Asian-Australasian Journal of Animal Sciences, 2015, 28, 9, 1274  crossref(new windwow)
Baah, J., J. A. Shelford, A. N. Hristov, T. A. McAllister and K.-J. Cheng. 2005. The effects of Tween 80 and fibrolytic enzymes on ruminal fermentation and digestibility of feeds in Holstein cows. Asian-Aust. J. Anim. Sci. 18:816-824. crossref(new window)

Cai, Y. 1999. Identification and characterization of Enterococcus species isolated from forage crops and their influence on silage fermentation. J. Dairy Sci. 82:2466-2471. crossref(new window)

Canadian Council on Animal Care. 1993. A guide to the care and use of experimental animals. Vol. 1, 2nd edn. (Ed. E. D. Olfert, B. M. Cross and A. A. McWilliams). CCAC, Ottawa, ON.

Fitzsimmons, A., F. Duffner, D. Curtin, G. Brophy, P. O'Kiely and M. O'Connel. 1992. Assessment of Pediococcus acidilactici as a potential silage inoculant. Appl. Environ. Microbiol. 58:3047-3052.

Hristov, A. N. and T. A. McAllister. 2002. Effect of inoculants on whole-crop barley silage fermentation and dry matter disappearance in situ. J. Anim. Sci. 80:510-516.

Inglis, G. D., L. J. Yanke, L. M. Kawchuk and T. A. McAllister. 1999. The influence of bacterial inoculants on the microbial ecology of aerobic spoilage of barley silage. Can. J. Microbiol. 45:77-87. crossref(new window)

Jung, H. G. 1997. Analysis of forage fibre and cell walls in ruminant nutrition. J. Nutr. 127:810S-813S.

Kamande, G. M. 1994. Manipulation of rumen and silage fermentation. PhD Thesis. Univ. British Columbia, Vancouver, British Columbia, Canada.

Keady, T. W. J. and R. W. J. Steen. 1994. Effects of treating low dry-matter grass with a bacterial inoculant on the intake and performance of beef cattle and studies on its mode of action. Grass Forage Sci. 49:438-446. crossref(new window)

Kim, W., Y. Gamo, Y. M. Sani, Y. Wusiman, S. Ogawa, S. Karita and M. Goto. 2006. Effect of Tween 80 on hydrolytic activity and substrate accessibility of carbohydrolase I (CBH I) from Trichoderma viride. Asian-Aust. J. Anim. Sci. 19:684-689. crossref(new window)

Kung, Jr., L., C. L. Myers, J. M. Neylon, C. C. Taylor, J. Lazartic, J. A. Mills and A. G. Whiter. 2004. The effects of buffered propionic acid-based additives alone or combined with microbial inoculation on the fermentation of high moisture corn and whole-crop barley. J. Dairy Sci. 87:1310-1316. crossref(new window)

Lee, C. H., H. G. Sung, M. Eslami, S. Y. Lee, J. Y. Song, S. S. Lee and J. K. Ha. 2007. Effects of Tween 80 pretreatment on dry matter disappearance of rice straw and cellulolytic bacterial adhesion. Asian-Aust. J. Anim. Sci. 20:1397-1401. crossref(new window)

Lee, S. S., H. S. Kim, Y. H. Moon, N. J. Choi and J. K. Ha. 2004. The effects of a non-ionic surfactant on the fermentation characteristics, microbial growth, enzyme activity and digestibility in the rumen of cows. Anim. Feed Sci. Technol. 115:37-50. crossref(new window)

McAllister, T. A. and A. N. Hristov. 1999. Silage inoculants and enzymes - practical uses and comparisons. Proceedings of the 20th Western Nutrition Conference, Calgary, Canada. pp. 63-75.

McAllister, T. A., R. Feniuk, Z. Mir, P. Mir, L. B. Selinger and K.-J. Cheng. 1998. Inoculants for alfalfa silage: effects on aerobic stability, digestibility and growth performance of feedlot steers. Livest. Prod. Sci. 53:171-181. crossref(new window)

McAllister, T. A., L. B. Selinger, L. R. McMahon, H. D. Bae, T. J. Lysyk, S. J. Oosting and K.-J. Cheng. 1995. Intake, digestibility and aerobic stability of barley silage inoculated with mixtures of Lactobacillus plantarum and Enterococcus faecium. Can. J. Anim. Sci. 75:425-432. crossref(new window)

McAllister, T. A., K. Stanford, H. D. Bae, R. J. Treacher, A. N. Hristov, J. Baah, J. A. Shelford and K.-J. Cheng. 2000. Effect of a surfactant and exogenous enzymes on digestibility of feed and on growth performance and carcass traits of lambs. Can. J. Anim. Sci. 80:35-44. crossref(new window)

Monteils, V. and S. Jurjanz. 2005. Ruminal degradability of corn forages depending on the processing method employed. Anim. Res. 54:3-15. crossref(new window)

Moshtaghi Nia, S. A. and K. M. Wittenberg. 1999. Use of forage inoculants with or without enzymes to improve preservation and quality of whole crop barley forage ensiled as large bales. Can. J. Anim. Sci. 79:525-532. crossref(new window)

Muck, R. E. 1996. Inoculation of silage and its effects on silage quality. Proceedings of the Informational Conference on Dairy and Forage Industry. Dairy Research Centre, Madison, Wisconsin, USA. pp. 43-51.

Muck, R. E. 2004. Effects of corn silage inoculants on aerobic stability. Trans. ASAE 47:1011-1016. crossref(new window)

Pauly, T. M. and P. Lingvall. 1999. Effects of mechanical forage treatment and surfactants on fermentation of grass silage. Acta Agric. Scand., Sect. A, Anim. Sci. 49:197-205.

Rahman, P. K. S. M. and E. Gakpe. 2008. Production, characterisation and application of biosurfactants - Review. Biotechnology 7:360-380. crossref(new window)

Reich, L. J. and L. Kung, Jr. 2010. Effects of combining Lactobacillus buchneri 40788 with various lactic acid bacteria on the fermentation and aerobic stability of corn silage. Anim. Feed Sci. Technol. 159:105-109. crossref(new window)

SAS 2006. SAS for mixed models. 2nd edn. SAS Institute Inc., Cary, North Carolina.

Singh, A., J. D. van Hamme and O. P. Ward. 2006. Surfactants in microbiology and biotechnology: Part 2. Application aspects. Biotechnol. Adv. 25:99-121.

Sucu, E. and I. Filya. 2006. Effects of bacterial inoculants on fermentation, aerobic stability and rumen degradability characteristics of wheat silages. Turk. J. Vet. Anim. Sci. 30:187-193.

van Hamme, J. D., A. Singh and O. P. Ward. 2006. Surfactants in microbiology and biotechnology: Part 1. physiological aspects. Biotechnol. Adv. 24:604-620. crossref(new window)

van Soest, P. J. 1982. Nutritional ecology of the ruminant. O and B Books Inc. Corvallis, Oregon, USA. pp. 81-82.

Weinberg, Z. G. and R. E. Muck. 1996. New trends and opportunities in the development and use of inoculants for silage. FEMS Microbiol. Rev. 19:53-68. crossref(new window)

Zahiroddini, H., J. Baah and T. A. McAllister. 2006. Effect of microbial inoculants on fermentation, nutrient retention and aerobic stability of barley silage. Asian-Aust. J. Anim. Sci. 19:1429-1436. crossref(new window)

Zahiroddini, H., J. Baah, W. Absalom and T. A. McAllister. 2004. Effect of an inoculant and hydrolytic enzymes on fermentation characteristics and nutritive value of whole crop barley silage. Anim. Feed Sci. Technol. 117:317-330. crossref(new window)