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Effects of the Combined Stress Induced by Stocking Density and Feed Restriction on Hematological and Cytokine Parameters as Stress Indicators in Laying Hens
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 Title & Authors
Effects of the Combined Stress Induced by Stocking Density and Feed Restriction on Hematological and Cytokine Parameters as Stress Indicators in Laying Hens
Kang, Sun-Young; Ko, Young-Hyun; Moon, Yang-Soo; Sohn, Sea-Hwan; Jang, In-Surk;
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A study was conducted to investigate the effects of the combined stressor induced by high stocking density with feed restriction on immunological parameters such as leukocyte differential counts and cytokine expression in laying hens. A total of forty White Leghorn laying hens were randomly allotted into the control (12 kg of body weight/) and the stress (44 kg of body weight/) groups, and then birds of the stress group were given 75% of voluntary intake of the control birds for 12-d on a daily basis. There was a significant decrease in body weight without affecting the relative weights of the liver and spleen after 12-d of the combined stressor. In hematological values, no significant difference in leukocyte differential counts including heterophils (H), lymphocytes (L), monocytes and H:L ratio was observed between the two groups. In cytokines, hepatic lipopolysaccharide-induced tumor necrosis factor- (LITNF-) and inducible nitric oxide synthase (iNOS) expression levels in the stress group were significantly (p<0.05) higher compared with those in the control group. However, the expression levels of interleukin (IL)-4 and IL-6 in the liver were not affected by the combined stressor. Splenic LITNF- expression in the combined stressor group was significantly (p<0.05) up-regulated compared with that in the control birds. However, the combined stressor did not affect splenic IL-4, IL-6 and iNOS expression. In conclusion, the combined stressor caused by high stocking density with feed restriction enhanced some pro-inflammatory cytokines including LITNF- and iNOS in lymphoid and non-lymphoid organs of birds, suggesting that altered cytokine expression to given stressors can be another parameter that can be used in assessing stress responses of birds.
The Combined Stressor;Stocking Density;Feed Restriction;Hematological Values;Cytokines;Laying Hens;
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Andrews, S. M., H. M. Omed and C. J. Phillips. 1997. The effect of a single or repeated period of high stocking density on the behavior and response to stimuli in broiler chickens. Poult. Sci. 76(12):1655-1660. crossref(new window)

Belmore, J., H. K. Kang, Y. J. Kim, V. K. Subramani, I. S. Jang, S. H. Sohn and Y. S. Moon. 2010. The effect of stocking density on stress related genes and telomeric broiler chickens. Asian-Aust. J. Anim. Sci. 23:437-443. crossref(new window)

Buijs, S., L. Keeling, S. Rettenbacher, E. Van Poucke and F. A. Tuyttens. 2009. Stocking density effects on broiler welfare: identifying sensitive ranges for different indicators. Poult. Sci. 88(8):1536-1543. crossref(new window)

Cook, M. E. 1991. Nutrition and immune response of the domestic fowl. CRC Crit. Rev. Poult. Biol. 3:167-189.

Delezie, E., Q. Swennen, J. Buyse and E. Decuypere. 2007. The effect of feed withdrawal and crating density in transit on metabolism and meat quality of broilers at slaughter weight. Polut. Sci. 86:1414-1423. crossref(new window)

de Jong, I. C., A. S. van Voorst and H. J. Blokhuis. 2003. Parameters for quantification of hunger in broiler breeders. Physiol. Behav. 78:773-783. crossref(new window)

de Jong, I. C., S. van Voorst, D. A. Ehlhardt and H. J. Blokhuis. 2002. Effects of restricted feeding on physiological stress parameters in growing broiler breeders. Br. Poult. Sci. 43(2):157-168. crossref(new window)

Fassbinder-Orth, C. A. and W. H. Karasov. 2006. Effects of feed restriction and realimentation on digestive and immune function in the leghorn chick. Poult. Sci. 80:1449-1456.

Felten, S. Y., K. S. Madden, D. L. Bellinger, B. Kruszewska, J. A. Moynihan and D. L. Felten. 1998. The role of the sympathetic nervous system in the modulation of immune responses. Adv. Pharmacol. 42:583-587.

Guzik, T. J., R. Korbut and T. Adamek-Guzik. 2003. Nitric oxide and superoxide in inflammation and immune regulation. J. Physiol. Pharmacol. 54(4):469-487.

Hangalapura, B. N., M. G. Kaiser, J. J. Poel, H. K. Parmentier and S. J. Lamont. 2006. Cold stress equally enhances in vivo pro-inflammatory cytokine gene expression in chicken lines divergently selected for antibody responses. Dev. Comp. Immunol. 30(5):503-511. crossref(new window)

Hocking, P. M., M. H. Maxwell and M. A. Mitchell. 1994. Haematology and blood composition at two ambient temperatures in genetically fat and lean adult broiler breeder females fed ad libitum or restricted throughout life. Br. Poult. Sci. 35(5):799-807. crossref(new window)

Hong, Y. H., H. S. Lillehoj, S. H. Lee, D. W. Park and E. P. Lillehoj. 2006. Molecular cloning and characterization of chicken lipopolysaccharide-induced TNF-alpha factor (LITAF). Dev. Comp. Immunol. 30(10):919-929. crossref(new window)

Huff, G. R., W. E. Huff, J. M. Balog, N. C. Rath, N. B. Anthony and K. E. Nestor. 2005. Stress response differences and disease susceptibility reflected by heterophil to lymphocyte ratio in turkeys selected for increased body weight. Poult. Sci. 84(5):707-717.

Janeway, C. A., P. Travers, M. Walport and M. Shlomchik. 2001. The adaptive immune responses. In; Immunology V (Ed. C. A. Janeway). Garland Publishing New York, NY. pp. 295-381.

Jang, I. S., S. Y. Kang, Y. H. Ko, Y. S. Moon and S. H. Sohn. 2009. Effect of qualitative and quantitative feed restriction on growth performance and immune function in broiler chickens. Asian-Aust. J. Anim. Sci. 22(3):388-395. crossref(new window)

Jolly, C. A. 2004. Dietary restriction and immune function. J. Nutr. 134(8):1853-1856.

Khajavi, M., S. Rahimi, Z. M. Hassan, M. A. Kamali and T. Mousavi. 2003. Effect of feed restriction early in life on humoral and cellular immunity of two commercial broiler strains under heat stress conditions. Br. Poult. Sci. 44(3):490-497. crossref(new window)

Liew, P. K., I. Zulkifli, M. Hair-Bejo, A. R. Omar and D. A. Israf. 2003. Effects of early age feed restriction and heat conditioning on heat shock protein 70 expression, resistance to infectious bursal disease, and growth in male broiler chickens subjected to heat stress. Poult. Sci. 82(12):1879-1885. crossref(new window)

Mashaly, M. M., G. L. 3rd Hendricks, M. A. Kalama, A. E. Gehad, A. O. Abbas and P. H. Patterson. 2004. Effect of heat stress on production parameters and immune responses of commercial laying hens. Poult. Sci. 83(6):889-894. crossref(new window)

Mashaly, M. M., M. L. Webb, S. L. Youtz, W. B. Roush and H. B. Graves. 1984. Changes in serum corticosterone concentration of laying hens as a response to increased population density. Poult. Sci. 63(11):2271-2274. crossref(new window)

Maxwell, M. H., G. W. Robertson, S. Spence and C. C. McCorquodale. 1990. Comparison of hematological values in restricted- and ad libitum-fed domestic fowls: red blood cell characteristics. Br. Poult. Sci. 31(2):407-413. crossref(new window)

McFarlane, J. M. and S. E. Curtis. 1989. Multiple concurrent stressors in chicks. 3. Effects on plasma corticosterone and the heterophil:lymphocyte ratio. Poult. Sci. 68(4):522-527. crossref(new window)

Mtileni, B. J., K. A. Nephawe and A. E. Nesamvuni. 2007. The influence of stocking density on body weight, egg weight, and feed intake of adult broiler breeder hens. Poult. Sci. 86:1615-1619. crossref(new window)

Nicol, C. J., S. N. Brown, E. Glen, S. J. Pope, F. J. Short, P. D. Warriss, P. H. Zimmerman and L. J. Wilkins. 2006. Effects of stocking density, flock size and management on the welfare of laying hens in single-tier aviaries. Br. Poult. Sci. 47(2):135-146. crossref(new window)

Ozkan, S., I. Plavnik and S. Yahav. 2006. Effects of early feed restriction on performance and asscites development in broiler chickens subsequently raised at low ambient temperature. J. Appl. Poult. Res. 15:9-19. crossref(new window)

Patterson, P. H. and H. S. Siegel. 1998. Impact of cage density on pullet performance and blood parameters of stress. Poult. Sci. 77(1):32-40 crossref(new window)

Puvadolpirod, S. and J. P. Thaxton. 2000. Model of physiological stress in chickens 1. Response parameters. Poult. Sci. 79(3):363-369. crossref(new window)

SAS. 1989. SAS/STAT User's Guide, Version 6, 4th Edition. Vol 2. SAS Ins., Cary, NC.

Shi, X. and A. L. Nuttall. 2003. Upregulated iNOS and oxidative damage to the cochlear stria vascularis due to noise stress. Brain Res. 967(1-2):1-10. crossref(new window)

Shini, S. and P. Kaiser. 2009. Effects of stress, mimicked by administration of corticosterone in drinking water, on the expression of chicken cytokine and chemokine genes in lymphocytes. Stress 12(5):388-399. crossref(new window)

Star, L., M. G. B. Nieuwland, B. Kemp and H. K. Parmentier. 2007. Effect of single or combined climatic and hygienic stress on natural and specific humoral immune competence in four layer lines. Poult. Sci. 86:1894-1903. crossref(new window)

Thaxton, J. P., W. A. Dozier 3rd, S. L. Branton, G. W. Morgan, D. W. Miles, W. B. Roush, B. D. Lott and Y. Vizzier-Thaxton. 2006. Stocking density and physiological adaptive response of broilers. Poult. Sci. 85(5):819-824. crossref(new window)

Turkyilmaz, M. K. 2008. Effect of stocking density on stress reaction in broiler chickens during summer. Turk. J. Vet. Anim. Sci. 32(1):31-36.

Ubosi, C. O., W. B. Gross, P. B. Hamilton, M. Ehrich and P. B. Siegel. 1985. Aflatoxin effects in white Leghorn chickens selected for response to sheep erythrocyte antigen. 2. Serological and organ characteristics. Poult. Sci. 64(6):1071-1076. crossref(new window)

Zimmerman, P. H., A. C. Lindberg, S. J. Pope, E. Glen, J. E. Bolhuis and C. J. Nicol. 2006. The effect of stocking density, flock size and modified management on laying hen behavior and welfare in a non-cage system. Appl. Anim. Behav. Sci. 101:111-124. crossref(new window)