Advanced SearchSearch Tips
Oxidative Stress and Antioxidant Status during Transition Period in Dairy Cows
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Oxidative Stress and Antioxidant Status during Transition Period in Dairy Cows
Sharma, N.; Singh, N.K.; Singh, O.P.; Pandey, V.; Verma, P.K.;
  PDF(new window)
The study was conducted on 20 Holstein X Sahiwal cross bred dairy cows, with an average milk production of liters in days during a single lactation, that were divided in to two groups of 10 animals. We investigated the oxidative stress and antioxidant status during the transition period in dairy cows. In this study, plasma level of MDA was considered as an indicator of lipid peroxidation and SOD, catalase, GSH and GSHPx as antioxidants. The lipid peroxidation was significantly (p<0.001) higher in cows during early lactation as compared to the cows in advanced pregnancy. A significant positive correlation (r = +0.831, p<0.01) was determined between MDA and catalase in early lactating cows. In early lactating cows, blood glutathione was significantly lower than in advanced pregnant cows. However, early lactating cows showed non-significant negative correlation for all antioxidant enzymes with lipid peroxidation. In conclusion, dairy cows seemed to have more oxidative stress and low antioxidant defense during early lactation or just after parturition than advanced pregnant cows, and this appears to be the reason for their increased susceptibility to production diseases (e.g. mastitis, metritis, retention of fetal membranes etc.) and other health problems.
Antioxidative Enzymes;Oxidative Stress;Production Diseases;Transition Period;Dairy Cow;
 Cited by
Selenium Concentration and Glutathione Peroxidase (GSH-Px) Activity in Serum of Cows at Different Stages of Lactation, Biological Trace Element Research, 2012, 147, 1-3, 91  crossref(new windwow)
Heat shock protein 70, oxidative stress, and antioxidant status in periparturient crossbred cows supplemented with α-tocopherol acetate, Tropical Animal Health and Production, 2012, 45, 1, 239  crossref(new windwow)
Effects of Supplementary Selenium Source on the Blood Parameters in Beef Cows and Their Nursing Calves, Biological Trace Element Research, 2013, 152, 2, 204  crossref(new windwow)
Status of bovine mastitis and associated risk factors in subtropical Jeju Island, South Korea, Tropical Animal Health and Production, 2013, 45, 8, 1829  crossref(new windwow)
Effect of Supranutritional Organic Selenium Supplementation on Postpartum Blood Micronutrients, Antioxidants, Metabolites, and Inflammation Biomarkers in Selenium-Replete Dairy Cows, Biological Trace Element Research, 2014, 161, 3, 272  crossref(new windwow)
Amelioration of Reproduction-Associated Oxidative Stress in a Viviparous Insect Is Critical to Prevent Reproductive Senescence, PLoS ONE, 2014, 9, 4, e87554  crossref(new windwow)
Oxidative stress and antioxidant status in dairy cows during prepartal and postpartal periods, Acta Veterinaria Brno, 2015, 84, 2, 133  crossref(new windwow)
Digestibility, ruminal fermentation, blood metabolites and antioxidant status in ewes supplemented with DL-methionine or hydroxy-4 (methylthio) butanoic acid isopropyl ester, Journal of Animal Physiology and Animal Nutrition, 2016, 101, 5, e266  crossref(new windwow)
Bovine mastitis: prevalence, risk factors and isolation of Staphylococcus aureus in dairy herds at Hawassa milk shed, South Ethiopia, BMC Veterinary Research, 2016, 12, 1  crossref(new windwow)
Selenium and Antioxidant Status in Dairy Cows at Different Stages of Lactation, Biological Trace Element Research, 2016, 171, 1, 89  crossref(new windwow)
Liver transcriptome analysis reveals important factors involved in the metabolic adaptation of the transition cow, Journal of Dairy Science, 2017, 100, 11, 9311  crossref(new windwow)
Acetoacetate induces hepatocytes apoptosis by the ROS-mediated MAPKs pathway in ketotic cows, Journal of Cellular Physiology, 2017, 232, 12, 3296  crossref(new windwow)
Oxidative Stress, Hemato-biochemical and Plasma Mineral Profile in Transition Buffaloes, Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 2017, 87, 4, 1091  crossref(new windwow)
Aebi, H. 1983. Catalase. In Methods Enzymology (Ed. H. U. Berg Meyer). pp. 276-286. Academic Press, New York.

Aitken, S. L., E. L. Karcher, P. Rezamand, J. C. Gandy, M. J. Vandehaar, A. V. Capuco and L.M. Sordillo. 2009. Evaluation of antioxidant and proinflammatory gene expression in bovine mammary tissue during the transition period. J. Dairy Sci. 92:589-598. crossref(new window)

Allison, R. D. and R. A. Laven. 2000. Effect of vitamin E supplementation on the health and fertility of dairy cows: a review. Vet. Rec. 147:703-708.

Balasinska, B. 2004. Evaluation of antioxidant status in living organisms. Med. Weter. 60:579-583.

Bernabucci, U., B. Ronchi, N. Lacetera and A. Nardone. 2005. Influence of body condition score on relationships between metabolic status and oxidative stress in periparturient dairy cows. J. Dairy Sci. 88:2017-2026. crossref(new window)

Beutler, E. 1963. Red cell metabolism. In a manual of biochemical methods, pp. 67-69.

Formigoni, A., D. Calderone, P. Pezzi and A. Panciroli. 1997. Evolution of oxidative status in dairy cows: preliminary observations. In: Proceedings of the 12th Associazione Scientifica Produzioni Animali Congress, Pisa. pp. 203-204.

Gitto, E., R. J. Reiter, M. Karbownik, D. X. Tan, P. Gitto, S. Barberi and I. Barberi. 2002. Causes of oxidative stress in the pre- and perinatal period. Biol. Neonate 81:146-157. crossref(new window)

Goff, J. P. and R. L. Horst. 1997. Physiological changes at parturition and their relationship to metabolic disorders. J. Dairy Sci. 80:1260-1268. crossref(new window)

Hafeman, D. G., R. A. Sunde and W. G. Hoekstra. 1974. Effect of dietary selenium on erythrocyte and liver glutathione peroxidase in the rats. J. Nutr. 104:580-587.

Halliwell, B. and J. M. C. Gutteridege. 2007. Free radicals in biology and medicine, 4th Ed. Oxford University Press. Grune Strottan, New York.

Halliwell, B. and M. Whiteman. 2004. Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean?. Br. J. Pharmacol. 142:231-255. crossref(new window)

Holbrook, J. and C. L. Hicks. 1978. Variation of superoxide dismutase in bovine milk. J. Dairy Sci. 61:1072. crossref(new window)

Kale, M., N. Rathore, S. John and D. Bhatnagar. 1999. Lipid peroxidative damage on pyrethroid exposure and alterations in antioxidant status in rat erythrocytes: a possible involvement of reactive oxygen species. Toxicol. Lett. 105:197-205. crossref(new window)

Kumaraguruparan, R., R. Subapriya, J. Kabalimoorthy and S. Nagini. 2002. Antioxidant profile in the circulation of patients with fibroadenoma and adenocarcinoma of the breast. Clin. Biochem. 35:275-279. crossref(new window)

Machlin, L. J. and A. Bendich. 1987. Free radical tissue damage: protective role of antioxidant nutrients. Fed. Am. Soc. Exp. Biol. 1:441.

Madsen, F. C., R. E. Rompala and J. K. Miller. 1990. Effect of disease on the metabolism of essential trace elements: a role for dietary coordination complexes. Feed Manage. 41:20.

Madsen, F. C. 1991. Disease and stress: a reason to consider the use of organically complexed trace elements. In: Biotechnology in the Feed Industry. Proceedig of the Alltech 7th Annual Symposium. Alltech Tech. Publ., Nicholasville, KY. p. 147.

Marklund, S. and G. Marklund. 1974. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 47:469-476. crossref(new window)

Ohkawa, H., N. Ohishi and K. Yagi. 1979. Assay for lipid peroxides in animal tissues by Thiobarbituric acid reaction. Anal. Chem. 95:351-358.

Ronchi, B., U. Bernabucci, N. Lacetera and A. Nardone. 2000. Oxidative and metabolic status of high yielding dairy cows in different nutritional conditions during the transition period. In: Proceedings of 51st Annual Mtg. E.A.A.P., Vienna. p. 125.

Saleh, M., A. Salam and I. M. H. MEL Mileegy. 2007. Oxidative antioxidant status during transition from late pregnancy to early lactation in native and cross bred cows in the Egyptian oasis. Assiut. Vet. Med. J. 53:113.

Shanks, R. D., A. E. Freeman and F. N. Dickinson. 1981. Postpartum distribution of costs and disorders of health. J. Dairy Sci. 64:683. crossref(new window)

Snedecor, G. W. and W. G. Cochran. 1994. Statistical methods. 8th Ed. Iowa State University Press, Ames, Iowa, USA.

Sordillo, L. M. and S. L. Aitken. 2009. Impact of oxidative stress on the health and immune function of dairy cattle. Vet. Immunol. Immunopathol. 128:104-109. crossref(new window)

Sordillo, L. M., G. A. Contreras and S. L. Aitken. 2009. Metabolic factors affecting the inflammatory response of periparturient dairy cows. Anim. Health Res. Rev. 10:53-63. crossref(new window)

Sordillo, L. M., N. O'Boyle, J. C. Gandy, C. M. Corl and E. Hamilton. 2007. Shifts in thioredoxin reductase activity and oxidant status in mononuclear cells obtained from transition dairy cattle. J. Dairy Sci. 90:1186-1192. crossref(new window)

Sordillo, L. M. 2005. Factors affecting mammary gland immunity and mastitis susceptibility. Livest. Prod. Sci. 98:89-99. crossref(new window)

Trevisan, M., R. Browne, M. Ram, P. Muti, J. Freudenheim, A. N. Carosella and D. Armstrong. 2001. Correlates of markers of oxidative status in the general population. Am. J. Epidemiol. 154:348-356. crossref(new window)

Valko, M., D. Leibfritz, J. Moncol, M. T. Cronin, M. Mazur and J. Telser. 2007. Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell Biol. 39:44-84. crossref(new window)

Vannucchi, H., A. A. Jordoa-Junior, A. C. Iglessias, M. V. Morandi and P. G. Chiarello. 1997. Effects of different diatery concentrations of vitamin E on lipid peroxidation in rats. Arch. Latinoam. Nutr. 47:34-37.

Waller, K. P. 2000. Mammary gland immunology around parturition. Influence of stress, nutrition and genetics. Adv. Exp. Med. Biol. 480: 231-245.

Weiss, W. P., J. S. Hogan, D. A. Todhunter and K. L. Smith. 1997. Effect of vitamin E supplementation in diets with a low concentration of selenium on mammary gland health of dairy cows. J. Dairy Sci. 80:1728-1737. crossref(new window)