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Age Prediction in the Chickens Using Telomere Quantity by Quantitative Fluorescence In situ Hybridization Technique
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 Title & Authors
Age Prediction in the Chickens Using Telomere Quantity by Quantitative Fluorescence In situ Hybridization Technique
Kim, Y.J.; Subramani, V.K.; Sohn, S.H.;
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Telomeres are special structures at the ends of eukaryotic chromosomes. Vertebrate telomeres consist of tandem repeats of conserved TTAGGG sequence and associated proteins. Birds are interesting models for molecular studies on aging and cellular senescence because of their slow aging rates and longer life spans for their body size. In this longitudinal study, we explored the possibility of using telomeres as an age-marker to predict age in Single Comb White Leghorn layer chickens. We quantified the relative amount of telomeric DNA in isolated peripheral blood lymphocytes by the Quantitative Fluorescence in situ Hybridization technique on interphase nuclei (IQ FISH) using telomere-specific DNA probes. We found that the amount of telomeric DNA (ATD) reduced significantly with an increase in chronological age of the chicken. Especially, the telomere shortening rates are greatly increased in growing individuals compared to laying and old-aged individuals. Therefore, using the ATD values obtained by IQ FISH we established the possibility of age prediction in chickens based on the telomere theory of aging. By regression analysis of the ATD values at each age interval, we formulated an equation to predict the age of chickens. In conclusion, the telomeric DNA values by IQ FISH analyses can be used as an effective age-marker in predicting the chronological age of chickens. The study has implications in the breeding and population genetics of poultry, especially the reproductive potential.
Chickens;Telomere;Aging;Age Prediction;Fluorescence In situ Hybridization;
 Cited by
닭 텔로미어 길이의 유전력 추정과 유전 전이 양상,박단비;손시환;

한국가금학회지, 2014. vol.41. 3, pp.217-225 crossref(new window)
Inheritance and Heritability of Telomere Length in Chicken, Korean Journal of Poultry Science, 2014, 41, 3, 217  crossref(new windwow)
Dynamics of telomere length in the chicken, World's Poultry Science Journal, 2014, 70, 04, 721  crossref(new windwow)
Does Reproductive Investment Decrease Telomere Length in Menidia menidia?, PLOS ONE, 2015, 10, 5, e0125674  crossref(new windwow)
Allsopp, R. C., H. Vaziri, C. Patterson, S. Goldstein, E. V. Younglai, A. B. Futcher, C. W. Greider and C. B. Harley. 1992. Telomere length predicts replicative capacity of human fibroblasts. Proc. Natl. Acad. Sci. USA. 89:10114-10118. crossref(new window)

Aviv, A., W. Chen, J. P. Gardner, M. Kimura, M. Brimacombe, X. Cao, S. R. Srinivasan and G. S. Berenson. 2009. Leukocyte telomere dynamics: longitudinal findings among young adults in the Bogalusa Heart Study. Am. J. Epidemiol. 169:323-329.

Baird, D. M. and D. Kipling. 2004. The extent and significance of telomere loss with age. Ann. NY Acad. Sci. 1019:265-268. crossref(new window)

Bize, P., F. Criscuolo, N. B. Metcalfe, L. Nasir and P. Monaghan. 2009. Telomere dynamics rather than age predict life expectancy in the wild. Proc. Biol. Sci. 276:1679-1683. crossref(new window)

Blackburn, E. H. 1991. Telomeres. Trends Biochem. Sci. 16:378-381. crossref(new window)

Blackburn, E. H., S. Chan, J. Chang, T. B. Fulton, A. Krauskopf, M. McEachern, J. Prescott, J. Roy, C. Smith and H. Wang. 2000. Molecular manifestations and molecular determinants of telomere capping. Cold Spring Harb. Symp. Quant. Biol. 65:253-263. crossref(new window)

Campisi, J., S. H. Kim, C. S. Lim and M. Rubio. 2001. Cellular senescence, cancer and aging: the telomere connection. Exp. Gerontol. 36:1619-1637. crossref(new window)

Cawthon, R. M. 2002. Telomere measurement by quantitative PCR. Nucleic Acids Res. 30:e47. crossref(new window)

Cawthon, R. M. 2009. Telomere length measurement by a novel monochrome multiplex quantitative PCR method. Nucleic Acids Res. 37(3):e21. crossref(new window)

Cawthon, R. M., K. R. Smith, E. O'Brien, A. Sivatchenko and R. A. Kerber. 2003. Association between telomere length in blood and mortality in people aged 60 years or older. Lancet 361:393-395. crossref(new window)

Cho, E. J., C. H. Choi and S. H. Sohn. 2005. The amount of telomeres and telomerase activity on chicken embryonic cells during developmental stages. J. Anim. Sci. Technol. (Kor) 47:187-794. crossref(new window)

Counter, C. M., A. A. Avilion, C. E. LeFeuvre, N. G. Stewart, C. W. Greider, C. B. Harley and S. Bacchetti. 1992. Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity. EMBO J. 11:1921-1929.

Delany, M. E., L. M. Daniels, S. E. Swanberg and H. A. Taylor. 2003. Telomeres in the chicken: genome stability and chromosome ends. Poult. Sci. 82:917-926. crossref(new window)

Forsyth, N. R., W. E. Wright and J. W. Shay. 2002. Telomerase and differentiation in multicellular organisms: turn it off, turn it on, and turn it off again. Differentiation 69:188-197. crossref(new window)

Frenck, R. W. Jr., E. H. Blackburn and K. M. Shannon. 1998. The rate of telomere sequence loss in human leukocytes varies with age. Proc. Natl. Acad. Sci. USA. 95:5607-5610. crossref(new window)

Friedrich, U., E. Griese, M. Schwab, P. Fritz, K. Thon and U. Klotz. 2000. Telomere length in different tissues of elderly patients. Mech. Ageing Dev. 119:89-99. crossref(new window)

Harley, C. B. 1995. Telomeres and aging. In Telomeres (Ed. E. H. Blackburn and C. W. Greider). Cold Spring Harbor Laboratory Press, New York.

Harley, C. B., A. B. Futcher and C. W. Greider. 1990. Telomeres shorten during ageing of human fibroblasts. Nature 345:458-460. crossref(new window)

Hastie, N. D., M. Dempster, M. G. Dunlop, A. M. Thompson, D. K. Green and R. C. Allshire. 1990. Telomere reduction in human colorectal carcinoma and with ageing. Nature 346:866-868. crossref(new window)

Hastings, R., N. C. Li, P. S. Lacy, H. Patel, K. E. Herbert, A. G. Stanley and B. Williams. 2004. Rapid telomere attrition in cardiac tissue of the ageing Wistar rat. Exp. Gerontol. 39:855-857. crossref(new window)

Haussmann, M. F., C. M. Vleck and I. C. Nisbet. 2003. Calibrating the telomere clock in common terns, Sterna hirundo. Exp. Gerontol. 38:787-789. crossref(new window)

Haussmann, M. F., D. W. Winkler and C. M. Vleck. 2005. Longer telomeres associated with higher survival in birds. Biol. Lett. 1:212-214. crossref(new window)

Hewakapuge, S., R. A. van Oorschot, P. Lewandowski and S. Baindur-Hudson. 2008. Investigation of telomere lengths measurement by quantitative real-time PCR to predict age. Leg Med. (Tokyo) 10:236-242. crossref(new window)

Holmes, D. J. and S. N. Austad. 1995. Birds as animal models for the comparative biology of aging: a prospectus. J. Gerontol. A Biol. Sci. Med. Sci. 50:B59-66.

Holmes, D. J., S. L. Thomson, J. Wu and M. A. Ottinger. 2003. Reproductive aging in female birds. Exp. Gerontol. 38:751-756. crossref(new window)

Joeng, K. S., E. J. Song, K. J. Lee and J. Lee. 2004. Long lifespan in worms with long telomeric DNA. Nat. Genet. 36:607-611. crossref(new window)

Jung, G. S., E. J. Cho, D. S. Choi, M. J. Lee, C. Park, I. S. Jeon and S. H. Sohn. 2006. Analysis of telomere length and telomerase activity of tissues in Korean Native Chicken. Kor. J. Poul. Sci. 33:97-103.

Lavoie, E. T. and K. A. Grasman. 2005. Isolation, cryopreservation, and mitogenesis of peripheral blood lymphocytes from chickens (Gallus domesticus) and wild herring gulls (Larus argentatus). Arch. Environ. Contam. Toxicol. 48:552-558. crossref(new window)

McKevitt, T. P., L. Nasir, P. Devlin and D. J. Argyle. 2002. Telomere lengths in dogs decrease with increasing donor age. J. Nutr. 132:1604S-1606S.

McKevitt, T. P., L. Nasir, C. V. Wallis and D. J. Argyle. 2003. A cohort study of telomere and telomerase biology in cats. Am. J. Vet. Res. 64:1496-1499. crossref(new window)

Monnier, V. M. 1990. Nonenzymatic glycosylation, the Maillard reaction and the aging process. J. Gerontol. 45:B105-111. crossref(new window)

Moyzis, R. K., J. M. Buckingham, L. S. Cram, M. Dani, L. L. Deaven, M. D. Jones, J. Meyne, R. L. Ratliff and J. R. Wu. 1988. A highly conserved repetitive DNA sequence, (TTAGGG)n, present at the telomeres of human chromosomes. Proc. Natl. Acad. Sci. USA. 85:6622-6626. crossref(new window)

Munshi-South, J. and G. S. Wilkinson. 2010. Bats and birds: Exceptional longevity despite high metabolic rates. Ageing Res. Rev. 9:12-19. crossref(new window)

Nanda, I. and M. Schmid. 1994. Localization of the telomeric (TTAGGG)n sequence in chicken (Gallus domesticus) chromosomes. Cytogenet. Cell Genet. 65:190-193. crossref(new window)

Nasir, L., P. Devlin, T. McKevitt, G. Rutteman and D. J. Argyle. 2001. Telomere lengths and telomerase activity in dog tissues: a potential model system to study human telomere and telomerase biology. Neoplasia 3:351-359. crossref(new window)

O'Callaghan, N., V. Dhillon, P. Thomas and M. Fenech. 2008. A quantitative real-time PCR method for absolute telomere length. BioTechniques 44:807-809. crossref(new window)

Olovnikov, A. M. 1973. A theory of marginotomy. The incomplete copying of template margin in enzymic synthesis of polynucleotides and biological significance of the phenomenon. J. Theor. Biol. 41:181-190. crossref(new window)

Proctor, C. J. and T. B. Kirkwood. 2002. Modelling telomere shortening and the role of oxidative stress. Mech. Ageing Dev. 123:351-363. crossref(new window)

Ren, F., C. Li, H. Xi, Y. Wen and K. Huang. 2009. Estimation of human age according to telomere shortening in peripheral blood leukocytes of tibetan. Am. J. Forensic Med. Pathol. 30:252-255. crossref(new window)

Salomons, H. M., G. A. Mulder, L. van de Zande, M. F. Haussmann, M. H. Linskens and S. Verhulst. 2009. Telomere shortening and survival in free-living corvids. Proc. Biol. Sci. 276:3157-3165. crossref(new window)

Shiels, P. G., A. J. Kind, K. H. Campbell, D. Waddington, I. Wilmut, A. Colman and A. E. Schnieke. 1999. Analysis of telomere lengths in cloned sheep. Nature 399:316-317.

Slijepcevic, P. 2001. Telomere length measurement by Q-FISH. Methods Cell Sci. 23:17-22. crossref(new window)

Sohn, S. H., A. S. Multani P. K. Gugnani and S. Pathak. 2002. Telomere erosion-induced mitotic catastrophe in continuously grown chinese hamster don cells. Exp. Cell Res. 279:271-276. crossref(new window)

Sohn, S. H., E. J. Cho, W. J. Son and C. Y. Lee. 2007. Diagnosis of bovine freemartinism by fluorescence in situ hybridization on interphase nuclei using a bovine Y chromosome-specific DNA probe. Theriogenology 68:1003-1011. crossref(new window)

Solovei, I., E. R. Gaginskaya and H. C. Macgregor. 1994. The arrangement and transcription of telomere DNA sequences at the ends of lampbrush chromosomes of birds. Chromosome Res. 2:460-470. crossref(new window)

Swanberg, S. E. and M. E. Delany. 2005. Differential expression of genes associated with telomere length homeostasis and oncogenesis in an avian model. Mech. Ageing Dev. 126:1060-1070. crossref(new window)

Taylor, H. A. and M. E. Delany. 2000. Ontogeny of telomerase in chicken: impact of downregulation on pre- and postnatal telomere length in vivo. Dev. Growth Differ. 42:613-621. crossref(new window)

Thomas, P., N. J. O'Callaghan and M. Fenech. 2008. Telomere length in white blood cells, buccal cells and brain tissue and its variation with ageing and Alzheimer's disease. Mech. Ageing Dev. 129:183-190. crossref(new window)

Tsuji, A., A. Ishiko, T. Takasaki and N. Ikeda. 2002. Estimating age of humans based on telomere shortening. Forensic Sci. Int. 126:197-199. crossref(new window)

Vaziri, H., F. Schachter, I. Uchida, L. Wei, X. Zhu, R. Effros, D. Cohen and C. B. Harley. 1993. Loss of telomeric DNA during aging of normal and trisomy 21 human lymphocytes. Am. J. Hum. Genet. 52:661-667.