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Effect of Single Nucleotide Polymorphisms of Acetyl-CoA Carboxylase α(ACACA) Gene on Carcass Traits in Hanwoo (Korean Cattle)
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 Title & Authors
Effect of Single Nucleotide Polymorphisms of Acetyl-CoA Carboxylase α(ACACA) Gene on Carcass Traits in Hanwoo (Korean Cattle)
Shin, Sung-Chul; Heo, Jae-Pil; Chung, Eui-Ryong;
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Meat production and quality traits in beef cattle are largely affected by genetic factors. Acetyl-Coenzyme A carboxylase- (ACACA) plays a key role in the regulation and metabolism of fatty acid biosynthesis in mammalian animals. The gene encoding ACACA enzyme was chosen as a candidate gene for carcass and meat traits. In this study, we investigated effects of single nucleotide polymorphisms (SNPs) in the ACACA gene on beef carcass and meat traits in Hanwoo (Korean cattle) populations. We have sequenced a fragment of intron I region of the Hanwoo ACACA gene and identified two SNPs. Genotyping of the two SNP markers (g.2344T>C and g.2447C>A) was carried out using PCR-SSCP analysis in 309 Hanwoo steers to evaluate their association with carcass and meat production traits. The g.2344C SNP marker showed a significant increasing effect on LW (p = 0.009) and CW (p = 0.017). Animals with the CC genotype had higher CW and LW compared with TT and TC genotypes (p<0.05). The g.2447A SNP marker was associated with higher MC (p = 0.019). Animals with the AA genotype had higher MC than animals with CC and CA genotypes (p<0.05). Although the degree of linkage disequilibrium (LD) was not strong between g.2344T>C and g.2447C>A in the LD analysis, four major haplotype classes were formed with two SNP information within the ACACA gene. We constructed haplotypes using the HaploView software package program and analyzed association between haplotypes and carcass traits. The haplotype of ACACA gene significantly affected the LW (p = 0.027), CW (p = 0.041) and MC (p = 0.036). The effect of h1 haplotype on LW and CW was larger than that of h3 haplotype. Animals with the h1 haplotype also had greater MC than did animals with h2 haplotype. Consequently, the ACACA gene could be useful as a DNA marker for meat production traits such as carcass yield and meat contents in Hanwoo.
Acetyl-CoA Carboxylase Gene;SNP Marker;Carcass Traits;Hanwoo;
 Cited by
한우의 Fatty Acid Synthase (FASN)와 Acetyl CoA Carboxylase-α (ACACA) 유전자내의 단일염기변이가 한우집단내의 도체형질에 미치는 영향,전은경;김상욱;최윤정;김내수;조만욱;이명일;정용호;이진석;김관태;고경철;김관석;

Journal of Animal Science and Technology, 2011. vol.53. 5, pp.389-395 crossref(new window)
Effect of the Fatty Acid Synthase and Acetyl CoA Carboxylase Genes on Carcass Quality in Commercial Hanwoo Population, Journal of Animal Science and Technology, 2011, 53, 5, 389  crossref(new windwow)
Abu-Elheiga, L., A. Jayakumar, A. Baldini, S. S. Chirala and S. J. Wakil. 1995. Human acetyl-CoA carboxylase: characterization, molecular cloning, and evidence for two isoforms. Proc. Natl. Acad. Sci. 92:4011-4015. crossref(new window)

Arnyasi, M., E. Grindflek, A. Javor and S. Lien. 2006. Investigation of two candidate genes for meat quality traits in a quantitative trait locus region on SSC6: the porcine short heterodimer partner and heart fatty acid binding protein genes. J. Anim. Breed. Genet. 123:198-203. crossref(new window)

Badaoui, B., J. M. Serradilla, A. Tomas, B. Urrutia, J. L. Ares, J. Carrizosa, A. Sanchez, J. Jordana and M. Amills. 2007. Goat acetylcoenzyme A carboxylase alpha: molecular characterization, polymorphism, and association with milk traits. J. Dairy Sci. 90:1039-1043. crossref(new window)

Barrett, J. C. and L. R. Cardon. 2006. Evaluating coverage of genome-wide association studies. Nat. Genet. 38:659-662. crossref(new window)

Bendixen, E. 2005. The use of proteomics in meat science. Meat Sci. 71:138-149. crossref(new window)

Falconer, D. S. and T. F. C. Mackay. 1996. Introduction to quantitative genetics. 4th Edition. Pearson Education Ltd., Essex, England.

Federica, S., N. Francesco, D. M. Giovanna, S. M. Carmela, C. Gennaro, T. Carmela and M. Bianca. 2008. Identification of novel single nucleotide polymorphisms in promoter III of the acetyl-CoA carboxylase-a gene in goats affecting milk production traits. J. Hered. 100:386-389.

Gao, Y., R. Zhang, X. Hu and N. Li. 2007. Application of genomic technologies to the improvement of meat quality of farm animals. Meat Sci. 77:36-45. crossref(new window)

Haegeman, A., J. L. Williams, A. Law, A. Van Zeveren and L. J. Peelman. 2003. Mapping and SNP analysis of bovine candidate genes for meat and carcass quality. Anim. Genet. 34:349-353. crossref(new window)

Mao, J., S. Marcos, S. K. Davis, J. Burzlaff and H. M. Seyfert. 2001. Genomic distribution of three promoters of the bovine gene encoding acetyl-CoA carboxylase alpha and evidence that the nutritionally regulated promoter I contains a repressive element different from that in rat. Biochem. J. 358:127-135. crossref(new window)

Miller, S. A., D. D. Dykes and H. F. Polesky. 1988. A simple salting out procedure for extracting DNA from human nucleated cells. Nucl. Acids Res. 16:1215. crossref(new window)

Moibi, J. A., E. D. Ekpe and R. J. Christopherson. 2000. Acetyl-CoA carboxylase and fatty acid synthase activity and immunodetectable protein in adipose tissues of ruminants: effect of temperature and feeding level. J. Anim. Sci. 78:2383-2392.

Moioli, B., F. Napolitano, L. Orru and G. Catillo. 2005. Single nucleotide polymorphism detection in promoter III of the acetyl-CoA carboxylase-a gene in sheep. J. Anim. Breed. Genet. 122:418-420. crossref(new window)

Pannier, L., T. Y. Sweene, R. M. Hamill, F. Ipek, P. C. Stapleton and A. M. Mullen. 2009. Lack of an association between single nucleotide polymorphisms in the bovine leptin gene and intramuscular fat in Bos taurus cattle. Meat Sci. 81:731-737. crossref(new window)

Ponce-Castaneda, M. V., F. Lopez-Casillas and K. H. Kim. 1991. Acetylcoenzyme A carboxylase messenger ribonucleic acid metabolism in liver, adipose tissues, and mammary glands during pregnancy and lactation. J. Dairy Sci. 74:4013-4021. crossref(new window)

Plastow, G. S., D. S. Carrión, M. Gil, J. A. García-Regueiro, M. Fonti Furnols, M., Gispert, M. A. Oliver, A. Velarde, M. D. Guàrdia, M. Hortós, M. A. Ruis, C. Sárraga, I. Díaz, A. Valero, A. Sosnicki, R. Klont, S. Dornan, J. M. Wilkinson, G. Evans, C. Sargent, G. Davey, D. Connolly, B. Houeix, C. M. Maltin, H. E. Hayes, V. Anandavijayan, A. Foury, N. Geverink, M. Cairns, R. E. Tilley, P. Mormède and S. C. Blott. 2005. Quality pork genes and meat production. Meat Sci. 70:409-421. crossref(new window)

SAS. 2008. SAS/STAT Software for PC. Release 9.2, SAS Institute Inc., Cary, NC, USA.

Travers, M. T. and M. C. Barber. 2001. Acetyl-CoA carboxylase-$\alpha$: Gene structure-function relationships. J. Anim. Sci. 79(E. Suppl.):E136-E143.

Zhang, S., T. J. Knight, J. M. Reecy, T. L. Wheeler, S. D. Shackelford, L. V. Cundiff and D. C. Beitz. 2009. Associations of polymorphisms in the promoter I of bovine acetyl-CoA carboxylase-$\alpha$ gene with beef fatty acid composition. Anim. Genet. 41:417-420.