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Evaluation of the Apparent Ileal Digestibility (AID) of Protein and Amino Acids in Nursery Diets by In vitro and In vivo Methods
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 Title & Authors
Evaluation of the Apparent Ileal Digestibility (AID) of Protein and Amino Acids in Nursery Diets by In vitro and In vivo Methods
Cho, J.H.; Kim, I.H.;
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The objective was to evaluate in vitro prediction of ileal digestibility of protein and amino acids (AA) for current nursery pig diets (n = 10) by using pepsin and pancreatin incubations. To compare in vivo ileal digestibility, forty nursery pigs (4 pigs per diet) with an initial BW of kg were surgically equipped with T-cannula in the distal ileum. In all cases, the values of in vitro digestibility were higher than those of in vivo digestibility (p<0.05). With regard to the relationships of essential and non essential AA (CP), the value was 0.76. With regard to AA, high relationships were observed in Ile, Thr, and Gly (0.85, 0.83, and 0.89, respectively). Also, there was a lower relationship for Arg, Met, Ala, Asp, Glu, Pro, Ser, and Tyr with values of 0.56, 0.54, 0.40, 0.54, 0.45, 0.24, 0.49, and 0.35, respectively between in vitro and in vivo digestibility. The EAA relationship ( = 0.71) was generally higher than that of NEAA ( = 0.50) numerically. In conclusion, there were strong linear relationships between in vivo and in vitro ileal digestibility (CP, Ile, Thr, and Gly). In vitro prediction of ileal digestibility (CP, Ile, Thr, and Gly) seems to have significant potential for practical application.
In vivo;In vitro;Ileal Digestibility;Nursery Pigs;
 Cited by
Boisen, S. 1991. A model for feed evaluation based on in vitro digestible dry matter and protein. In: In Vitro Digestion in Pigs and Poultry (Ed. M. F. Fuller). Commonwealth Agricultural Bureaux International. Slough. pp. 135-145.

Boisen, S. and B. O. Eggum. 1991. Critical evaluation of in vitro methods for estimating digestibility in simple-stomach animals. Nutr. Res. Rev. 4:141-162. crossref(new window)

Boisen, S. and J. A. Fernandez. 1995. Prediction of the apparent ileal digestibility of protein and amino acids in feedstuffs and feed mixtures for pigs by in vitro analyses. Anim. Feed Sci. Technol. 51:29-43. crossref(new window)

Boisen, S. and J. A. Fernandez. 1997. Prediction of the total tract digestibility of energy in feedstuffs and pig diets by in vitro analyses. Anim. Feed Sci. Technol. 68:277-286. crossref(new window)

Cone, J. W. and A. F. B. van der Poel. 1993. Prediction of apparent ileal digestibility in pigs with a two-step in-vitro method. J. Sci. Food Agric. 62:393-400. crossref(new window)

Duncan, D. B. 1995. Multiple range and multiple F test. Biometrics 11:1-42.

Huang, R. L., Z. L. Tan, T. X. Xing, Y. F. Pan and T. J. Li. 2000. An in vitro method for the estimation of ileal crude protein and amino acids digestibility using the dialysis tubing for pig feedstuffs. Anim. Feed Sci. Technol. 88:79-89. crossref(new window)

Jezierny, D., R. Mosenthin, N. Sauer and M. Eklund. 2010. In vitro prediction of standardised ileal crude protein and amino acid digestibilities in grain legumes for growing pigs. Animal 4:1987-1996. crossref(new window)

Kimura, F. and V. L. Miller. 1957. Improved determination of chromic oxide in cow feed and feces. J. Agric. Food Chem. 5:216. crossref(new window)

Noblet, J. and Y. Jaguelin-Peyraud. 2007. Prediction of digestibility of organic matter and energy in the growing pig from an in vitro method. Anim. Feed Sci. Technol. 134:211-222. crossref(new window)

NRC. 1998. Nutrient requirement of pigs. 10th Edition. National Research Council, Academy Press. Washington, DC.

Pujol, S. and D. Torrallardona. 2007. Evaluation of in vitro methods to estimate the in vivo nutrient digestibility of barley in pigs. Livest. Sci. 109:186-188. crossref(new window)

Regmi, P. R., W. C. Sauer and R. T. Zijlstra. 2008. Prediction of in vivo apparent total tract energy digestibility of barley in grower pigs using an in vitro digestibility technique. J. Anim. Sci. 86:2619-2626. crossref(new window)

Stein, H. H., C. F. Shipley and R. A. Easter. 1998. Technical Note: A technique for inserting a T-cannula into the distal ileum of pregnant sows. J. Anim. Sci. 76:1433-1436.

Wunsche, J., I. I. Herrman, M. Meinl, U. Hennig, F. Kreienbring and P. Zwierz. 1987. Einfluss exogener Faktoren auf die prazlkale Nlhrstoff- und aminosiurenre-sorption, ermittelt an Schweinen mit Ileo-Rectal-anastomosen. Tierernaehrung. 37:745-764. crossref(new window)