Advanced SearchSearch Tips
Maturational Changes in Binding Capacity of Fowl Sperm to the Epithelium of the Sperm Storage Tubules during Their Passage through the Male Reproductive Tract
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Maturational Changes in Binding Capacity of Fowl Sperm to the Epithelium of the Sperm Storage Tubules during Their Passage through the Male Reproductive Tract
Ahammad, Muslah U.; Okamoto, S.; Kawamoto, Yasuhiro; Nakada, T.;
  PDF(new window)
The objective of this study was to examine the binding potential of sperm to the epithelium of the sperm storage tubules (SST) in vitro and in vivo to assess the functional maturation of fowl sperm. Sperm from the testis, epididymis, as well as the proximal, middle and distal vas deferens were incubated in vitro with either the uterovaginal junction (UVJ)- or infundibular tissue containing SST at for 30 min. Aliquots of sperm were also artificially inseminated into the uteri of hens, and the UVJ and infundibulum were collected 24 h post artificial insemination (AI). After incubation and AI, tissues were washed to remove loosely adhered sperm and subjected to fluorescence staining with 4', 6-diamidino-2-phenylindole, dihydrochloride (DAPI) for counting the number of bound sperm per 0.25 mm2 of surface area. Sperm from the testis, epididymis, and the three segments of the vas deferens exhibited their differential (p<0.05) binding capacity, which increased gradually from the testicular to distal vas deferens sperm under both in vitro and in vivo conditions. Existing similar trend, sperm, regardless of their source had a lesser affinity to bind to the epithelium of the infundibular SST than to the UVJ-SST. These experimental results suggested that fowl sperm may undergo gradual changes in the process of functional maturation, whereby they gain the ability to bind to the epithelium of the SST during their passage through the male reproductive tract (MRT).
Binding Capacity;Sperm Storage Tubules;Maturation;Male Reproductive Tract;
 Cited by
Lectin staining of the uterovaginal junction and sperm-storage tubule epithelia in broiler hens, Poultry Science, 2016, 95, 4, 948  crossref(new windwow)
Maturational changes in the survivability and fertility of fowl sperm during their passage through the male reproductive tract, Animal Reproduction Science, 2011, 128, 1-4, 129  crossref(new windwow)
Ahammad, M. U., C. Nishino, H. Tatemoto, N. Okura, S. Okamoto, Y. Kawamoto and T. Nakada. 2011. Maturational changes in motility, acrosomal proteolytic activity, and penetrability of the inner perivitelline layer of fowl sperm, during their passage through the male genital tract. Theriogenology 76:1100-1109. crossref(new window)

Aitken, R. J. and J. S. Clarkson. 1988. Significance of reactive oxygen species and antioxidants in defining the efficacy of sperm preparation techniques. J. Androl. 9:367-376.

Ashizawa, K. and H. Nishiyama. 1983. Prolonged survival of fowl spermatozoa in the oviducal tissues in organ culture. Br. Poult. Sci. 24:27-32. crossref(new window)

Bakst, M. R. 1998. Structure of the avian oviduct with emphasis on sperm storage in poultry. J. Exp. Zool. 282:618-626. crossref(new window)

Bakst, M. R., G. J. Wishart and J. P. Brillard. 1994. Oviductal sperm selection, transport, and storage in poultry. Poult. Sci. Rev. 5:117-143.

Brillard, J. P. and M. R. Bakst. 1990. Quantification of spermatozoa in the sperm-storage tubules of turkey hens and the relation to sperm numbers in the perivitelline layer of eggs. Biol. Reprod. 43:271-275. crossref(new window)

Burrows, W. H. and J. P. Quinn. 1935. A method of obtaining spermatozoa from the domestic fowl. Poult. Sci. 14:251-254. crossref(new window)

Chalah, T., F. Seigneurin, E. Blesbois and J. P. Brillard. 1999. In vitro comparison of fowl sperm viability in ejaculates frozen by three different techniques and relationship with subsequent fertility in vivo. Cryobiology 39:185-191. crossref(new window)

Douard, V., D. Hermier, M. Magistrini, E. Blesbois. 2005. Role of seminal plasma in damage to turkey spermatozoa during in vitro storage. Theriogenology 63:126-137. crossref(new window)

Esponda, P. and J. M. Bedford. 1985. Surface of the rooster spermatozoa changes in passing through the Wolffian duct. J. Exp. Zool. 234:441-449. crossref(new window)

Howarth, B. Jr. 1990. Fertility following intrauterine insemination near the time of oviposition. Poult. Sci. 69:138-141. crossref(new window)

Howarth, B. Jr. 1983. Fertilizing ability of cock spermatozoa from the testis, epididymis and vas deferens following intramagnal insemination. Biol. Reprod. 28:586-590. crossref(new window)

Jones, R. C. and M. Lin. 1993. Spermatogenesis in birds. Oxf. Rev. Reprod. Biol. 15:233-64.

Lake, P. E. and O. Ravie. 1988. Effect of trypsin and neuraminidase on fowl spermatozoa and their retention in the oviduct. In: Proceedings of the 18th World's Poultry Congress, Nagoya, Japan. pp. 594-596.

Morris, S. A., B. Jr. Howarth, J. W. Crim, D. E. Rodriguez, S. Cordoba, P. Esponda, and J. M. Bedford. 1987. Specificity of sperm-binding Wolffian duct proteins in the rooster and their persistence on spermatozoa in the female host glands. J. Exp. Zool. 242:189-198. crossref(new window)

Pe'rez, F. A., S. M. Roma, M. O. Cabada and P. E. Marini. 2006. Sperm binding glycoprotein is differentially present surrounding the lumen of isthmus and ampulla of the pig's oviduct. Anat. Embryol. 211:619-624. crossref(new window)

Peterson, R. N., L. Russel, D. Bundman and M. Freund. 1980. Sperm-egg interaction: evidence for boar sperm plasma membrane receptors for porcine zona pellucida. Science 207:73-74. crossref(new window)

Petrunkina, A. M., R. Gehlhaar, W. Drommer, D. Waberski and E. Topfer-Petersen. 2001. Selective sperm binding to pig oviductal epithelium in vitro. Reproduction 121:889-896. crossref(new window)

R Development Core Team. 2008. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN 3-900051-0. (URL:

Robertson, L., G. J. Wishart and A. J. Horrocks. 2000. Identification of perivitelline N-linked glycans as mediators of sperm-egg interactions in chickens. Reproduction 120:397-403. crossref(new window)

Steel, M. G. and G. J. Wishart. 1996. Demonstration that the removal of sialic acid from the surface of chicken spermatozoa impedes their transvaginal migration. Theriogenology 46:1037-1044. crossref(new window)

Van Krey, H. P., F. X. Ogasawara and J. Pangborn. 1967. Light and electron microscopic studies of possible sperm gland emptying mechanisms. Poult. Sci. 46:69-78. crossref(new window)

Waberski, D., F. Magnus, F. Ardon, A. M. Petrunkina, K. F. Weitze and E. Topfer-Petersen. 2006. Binding of boar spermatozoa to oviductal epithelium in vitro in relation to sperm morphology and storage time. Reproduction 131:311-318. crossref(new window)

Zavaleta, D. and F. Ogasawara. 1987. A review of the mechanism of the release of spermatozoa from storage tubules in the fowl and turkey oviduct. World's Poult. Sci. J. 43:132-139 crossref(new window)