JOURNAL BROWSE
Search
Advanced SearchSearch Tips
In vitro-growth and Gene Expression of Porcine Preantral Follicles Retrieved by Different Protocols
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
In vitro-growth and Gene Expression of Porcine Preantral Follicles Retrieved by Different Protocols
Ahn, J.I.; Lee, S.T.; Park, J.H.; Kim, J.Y.; Park, J.H.; Choi, J.K.; Lee, G.; Lee, E.S.; Lim, J.M.;
  PDF(new window)
 Abstract
This study was conducted to determine how the isolation method of the porcine preantral follicles influenced the following follicular growth in vitro. Mechanical and enzymatical isolations were used for retrieving the follicles from prepubertal porcine ovaries, and in vitro-growth of the follicles and the expression of folliculogenesis-related genes were subsequently monitored. The enzymatic retrieval with collagenase treatment returned more follicles than the mechanical retrieval, while the percentage of morphologically normal follicles was higher with mechanical retrieval than with enzymatic retrieval. After 4 days of culture, mechanically retrieved, preantral follicles yielded more follicles with normal morphology than enzymatically retrieved follicles, which resulted in improved follicular growth. The mRNA expression of FSHR, LHR Cx43, DNMT1 and FGFR2 genes was significantly higher after culture of the follicles retrieved mechanically. These results suggest that mechanical isolation is a better method of isolating porcine preantral follicles that will develop into competent oocytes in in vitro culture.
 Keywords
Porcine;Preantral Follicle;Enzymatic Retrieval;Mechanical Retrieval;In vitro Culture;Follicular Growth;
 Language
English
 Cited by
 References
1.
Carrell, D. T., L. Liu, I. Huang and C. M. Peterson. 2005. Comparison of maturation, meiotic competence, and chromosome aneuploidy of oocytes derived from protocols for in vitro culture of mouse secondary follicles. J. Assist. Reprod. Genet. 9-10:347-534.

2.
Cozzi, E., E. Basio, M. Seveso, D. Rubello and E. Ancona. 2009. Xenotransplantation as a model of integrated, multidisciplinary research. Organogenesis 5:288-296.

3.
Demeestere, I., A. Delbaere, C. Gervy, M. Van Den Bergh, F. Devreker and Y. Englert. 2002 Effect of preantral follicle isolation technique on in-vitro follicular growth, oocyte maturation and embryo development in mice. Hum. Reprod. 17:2152-2159. crossref(new window)

4.
Eppig, J. J., M. O'Brien and K. Wigglesworth. 1996. Mammalian oocyte growth and development in vitro. Mol. Reprod. Dev.44:260-273. crossref(new window)

5.
Gougeon, A. 1996. Regulation of ovarian follicular development in primates: facts and hypotheses. Endocr. Rev. 17:121-155. crossref(new window)

6.
Hammer, C. 1998. Physiological obstacles after xenotransplantation. Ann. NY Acad. Sci. 862:19-27. crossref(new window)

7.
Iverson, W. O. and T. Talbot. 1998. Definition of production specification for xenotransplantation. Ann. NY Acad. Sci. 862: 121-124. crossref(new window)

8.
Lee, S. T., M. H. Choi, E. J. Lee, S. P. Gong, M. Jang, S. H. Park, H. Jee, D. Y. Kim, J. Y. Han and J. M. Lim. 2008. Establishment of autologous embryonic stem cells derived from preantral follicle culture and oocyte parthenogenesis. Fertil. Steril. 90:1910-1920. crossref(new window)

9.
Mao, J., G. Wu, M. F. Smith, T. C. McCauley, T. C. Cantley, R. S. Prather, B. A. Didion and B. N. Day. 2002. Effects of culture medium, serum type, and various concentrations of follicle-stimulating hormone on porcine preantral follicular development and antrum formation in vitro. Biol. Reprod. 67:1197-1203. crossref(new window)

10.
Mao, J., M. F. Smith, E. B. Rucker, G. M. Wu, T. C. McCauley, T. C. Cantley, R. S. Prather and B. N. Day. 2004. Effect of epidermal growth factor and insulin-like growth factor I on porcine preantral follicular growth, antrum formation, and stimulation of granulosa cell proliferation and suppression of apoptosis in vitro. J. Anim. Sci. 82:1967-1975.

11.
Orisaka, M., K. Tajima, B. K. Tsang and F. Kotsuji. 2009. Oocyte-granulosa-theca cell interaction during preantral follicular development. J. Ovarian Res. 9:2-9.

12.
Oxender, W. D., B. Colenbrander, D. F. M. Van de Wiel and C. J. G. Wensing. 1979. Ovarian development in fetal and prepubertal pigs. Biol. Reprod. 21:715-721. crossref(new window)

13.
Ozawa, M., T. Nagai, T. Somfai, M. Nakai, N. Maedomari, M. Fahrudin, N. W. Karja, H. Kaneko, J. Noguchi, K. Ohnuma, N. Yoshimi, H. Miyazaki and K. Kikuchi. 2008. Comparison between effects of 3-isobutyl-1-methylxanthine and FSH on gap junctional communication, LH-receptor expression, and meiotic maturation of cumulus-oocyte complexes in pigs. Mol. Reprod. Dev. 75:857-866. crossref(new window)

14.
Su, Y. Q., K. Suqiura and J. J. Eppig. 2009. Mouse oocyte control of granulose cell development and function: paracrine regulation of cumulus cell metabolism. Semin. Reprod. Med. 27:32-42. crossref(new window)

15.
Telfer, E. E. 1996. The development of methods for isolation and culture of preantral follicles from bovine and porcine ovaries. Theriogenology 45:101-110. crossref(new window)

16.
Wu, D., Q. C. Cheung, L. Wen and J. Li. 2006. A growth-maturation system that enhances the meiotic and developmental competence of porcine oocytes isolated from small follicles. Biol. Reprod. 75:547-554. crossref(new window)

17.
Wu, J., and Q. Tian. 2007b. Role of follicle stimulating hormone and epidermal growth factor in the development of porcine preantral follicle in vitro. Zygote 15:233-240. crossref(new window)

18.
Wu, J., B. R. Emery and D. T. Carrell. 2001. In vitro growth, maturation, fertilization, and embryonic development of oocytes from porcine preantral follicles. Biol. Reprod. 64:375-381. crossref(new window)

19.
Wu, J., B. Xu and W. Wang. 2007a. Effects of luteinizing hormone and follicle stimulating hormone on the developmental competence of porcine preantral follicle oocytes grown in vitro. J. Assist. Reprod. Genet. 24:419-24. crossref(new window)