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Effect of a c-MYC Gene Polymorphism (g.3350G>C) on Meat Quality Traits in Berkshire
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 Title & Authors
Effect of a c-MYC Gene Polymorphism (g.3350G>C) on Meat Quality Traits in Berkshire
Oh, J.D.; Kim, E.S.; Lee, H.K.; Song, K.D.;
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c-MYC (v-myelocytomatosis viral oncogene homologue) is a transcription factor that plays important role in many biological process including cell growth and differentiation, such as myogenesis and adipogenesis. In this study, we aimed to detect MYC gene polymorphisms, their genotype frequencies and to determine associations between these polymorphisms and meat quality traits in Berkshire pigs. We identified a single nucleotide polymorphism (SNP) in intron 2 of MYC gene by Sanger sequencing, i.e., g.3350G>C (rs321898326), that is only found in Berkshire pigs, but not in other breeds including Duroc, Landrace, and Yorkshire pigs that were used in this study. Genotypes of total 378 Berkshire pigs (138 sows and 240 boars) were determined using Hha I restriction enzyme digestion after polymerase chain reaction. Observed allele frequencies of GG, GC, and CC genotypes were 0.399, 0.508, and 0.093 respectively. Statistical analysis indicated that the g.3350G>C polymorphism was significantly associated with and cooking loss (p<0.05), suggesting that g.3350G>C SNP can be used for pre-selection of and cooking loss traits in Berkshire pigs.
Berkshire;Meat Quality Traits;v-Myelocytomatosis Viral Oncogene Homologue;Single Nucleotide Polymorphism;Association Analysis;
 Cited by
Andersson, L., C. S. Haley, H. Ellegren, S. A. Knott, M. Johansson, K. Andersson, L. Andersson-Eklund, I. Edfors-Lilja, M. Fredholm, I. Hansson, J. Hakansson, and K. Lundstrom. 1994. Genetic mapping of quantitative trait loci for growth and fatness in pigs. Science 263:1771-1774. crossref(new window)

Barge, M. T., G. Destefanis, G. P. Toscano, and A. Brugiapaglia. 1991. Two reading techniques of the filter paper press method for measuring meat water-holding capacity. Meat Sci. 29:183-199. crossref(new window)

Cameron, N. D. 1990. Genetic and phenotypic parameters for carcass traits, meat and eating quality traits in pigs. Livest. Prod. Sci. 26:119-135. crossref(new window)

Cepica, S., A. Stratil, M. Kopecny, P. Blazkova, J. Jr. Schroffel, R. Davoli, L. Fontanesi, G. Reiner, H. Bartenshlager, and G. Moser. 2003. Linkage and QTL mapping for Sus scrofa chromosome 4. J. Anim. Breed. Genet. 120:28-37. crossref(new window)

Cho, S. H., J. H. Kim, P. N. Seong, Y. M. Cho,W. T. Chung, B. Y. Park, M. O. Chung, D. H. Kim, J. M. Lee, and C. N. Ahn. 2008. Physico-chemical meat quality properties and nutritional composition of hanwoo steer beef with 1++ quality grade. Korean J. Food Sci. An. 28:422-430. crossref(new window)

Cliplef, R. L. and R. M. McKay. 1993. Carcass quality characteristics of swine selected for reduced backfat thickness and increased growth rate. Can. J. Anim. Sci. 73:483-494. crossref(new window)

Collier, J. J., T. T. Doan, M. C. Daniels, J. R. Schurr, J. K. Kolls, and D. K. Scott. 2003. c-Myc is required for the glucose-mediated induction of metabolic enzyme genes. J . Biol. Chem. 278: 6588-6595. crossref(new window)

Crawford, S. M., S. J. Moeller, H. M. Zerby, K. M. Irvin, P. S. Kuber, S. G. Velleman, and T. D. Leeds. 2010. Effects of cooked temperature on pork tenderness and relationships among muscle physiology and pork quality traits in loins from Landrace and Berkshire swine. Meat Sci. 84:607-612. crossref(new window)

Do, C. H., B. W. Cho, and D. H. Lee. 2012. Study on the prolactin receptor 3 (PRL3) gene and the retinol-binding protein 4 (RBP4) gene as candidate genes for production traits in Berkshire pigs. Asian Australas. J. Anim. Sci. 25:183-188.

de Koning, D. J., A. P. Rattink, B. Harlizius, M. A. M. Groenen, E. W. Brascamp, and J. A. M. van Arendonk. 2001. Detection and characterization of quantitative trait loci for growth and reproduction traits in pigs. Livest. Prod. Sci. 72:185-198. crossref(new window)

Edwards, D. B., C. W. Ernst, R. J. Tempelman, G. J. M. Rosa, N. E. Raney, M. D. Hoge, and R. O. Bates. 2008. Quantitative trait loci mapping in an F2 Duroc$\times$Pietrain resource population: I. Growth traits. J. Anim. Sci. 86:241-253.

Freytag, S. O. and T. J. Geddes. 1992. Reciprocal regulation of adipogenesis by Myc and C/EBP alpha. Science 256:379-382. crossref(new window)

Huff-Lonergan E., T. J. Baas, M. Malek, J. C. Dekkers, K. Prusa, and M. F. Rothschild. 2002. Correlations among selected pork quality traits. J. Anim. Sci. 80:617-27.

Jeong, D. W., Y. M. Choi, S. H. Lee, J. H. Choe, K. C. Hong, H. C. Park, and B. C. Kim. 2010. Correlations of trained panel sensory values of cooked pork with fatty acid composition, muscle fiber type, and pork quality characteristics in Berkshire pigs. Meat Sci. 86:607-615. crossref(new window)

Jung, J. H., C. W. Kim, B. Y. Park, J. S. Choi, and H. C.Park. 2011. Genetic parameter estimates for meat quality traits in berkshire pigs. J. Anim. Sci. Technol. 53:289-296. crossref(new window)

Kang, Y. K., Y. M. Choi, S. H. Lee, J. H. Choe, K. C. Hong, and B. C. Kim. 2011. Effects of myosin heavy chain isoforms on meat quality, fatty acid composition, and sensory evaluation in Berkshire pigs. Meat Sci. 89:384-389. crossref(new window)

Kim, C. J. and E. S. Lee. 2003. Effects of quality grade on the chemical, physical and sensory characteristics of Hanwoo (Korean native cattle) beef. Meat Sci. 63:397-405. crossref(new window)

Kipshidze, N., P. Iversen, E. Keane, D. Stein, P. Chawla, V. Skrinska, L. R. Shankar, R. Mehran, V. Chekanov, G. Dangas, R. Komorowski, C. Haudenschild, A. Khanna, M. Leon, M. H. Keelan, and J. Mose. 2002. Complete vascular healing and sustained suppression of neointimal thickening after local delivery of advanced c-myc antisense at six months follow-up in a rabbit balloon injury model. Cardiovasc. Radiat. Med. 3:26-30. crossref(new window)

Lee, E. A., J. M. Kim, K. S. Lim, Y. C. Ryu, W. M. Jeon, and K. C. Hong. 2012. Effects of variation in porcine MYOD1 gene on muscle fiber characteristics, lean meat production, and meat quality traits. Meat Sci. 92:36-43. crossref(new window)

Lee, S. H., Y. M. Choi, J. H. Choe, J. M. Kim, K. C. Hong, H. C. Park, and B. C. Kim. 2010. Association between polymorphisms of the heart fatty acid binding protein gene and intramuscular fat content, fatty acid composition, and meat quality in Berkshire breed. Meat Sci. 86:794-800. crossref(new window)

Levens, D., R. C. Duncan, T. Tomonaga, G. A. Michelotti, I. Collins, T. Davis-Smyth, T. Zheng, and E. F. Michelotti. 1997. DNA conformation, topology, and the regulation of c-myc expression. Curr. Top. Microbiol. Immunol. 224:33-46.

Liu, G., D. G. Jennen, E. Tholen, H. Juengst, T. Kleinwachter, M. Holker, D. Tesfaye, G. Un, H. J. Schreinemachers, E. Murani, S. Ponsuksili, J. J. Kim, K. Schellander, and K. Wimmers. 2007. A genome scan reveals QTL for growth, fatness, leanness and meat quality in a Duroc-Pietrain resource population. Anim. Genet. 38:241-252. crossref(new window)

MacDougald, O. A. and M. D. Lane. 1995. Transcriptional regulation of gene expression during adipocyte differentiation. Annu. Rev. Biochem. 64:345-373. crossref(new window)

Marklund, L., P. E. Nystrom, S. Stern, L. Andersson-Eklund, and L. Andersson. 1999. Confirmed quantitative trait loci for fatness and growth on pig chromosome 4. Heredity 82:134-141. crossref(new window)

Miner, J. H. and B. J. Wold. 1991. c-myc inhibition of MyoD and myogenin-initiated myogenic differentiation. Mol. Cell. Biol. 11:2842-2851. crossref(new window)

Ninomiya-Tsuji, J., F. M. Torti, and G. M. Ringold. 1993. Tumor necrosis factor-induced c-myc expression in the absence of mitogenesis is associated with inhibition of adipocyte differentiation. Proc. Natl. Acad. Sci. USA. 90:9611-9615. crossref(new window)

Oh, J. D., K. D. Song, J. H. Seo, D. K. Kim, S. H. Kim, K. S. Seo, H. T. Lim, J. B. Lee, H. C. Park, Y. C. Ryu, M. S. Kang, S. Cho, E. S. Kim, H. S. Choe, H. S. Kong, and H. K. Lee. 2014. Genetic traceability of black pig meats using microsatellite markers. Asian Australas. J. Anim. Sci. 27:926-931. crossref(new window)

Park, B. Y., S. H. Cho, J. H. Kim, P. N. Seong, G. H. Kang, D. W. Jeong, C. Kim, H. C. Park, J. H. Jeong, J. S. Choi, and D. H. Kim. 2010. Comparison of pork quality by different berkshire line. Korean J. Food Sci. Ani. Resour. 30:867-871. crossref(new window)

Potter, M. and K. B. Marcu.1997. The c-myc story: Where we've been, where we seem to be going. Curr. Top. Microbiol. Immunol. 224:1-17.

Reiner, G., W. Hecht, T. Leeb, B. Brenig, A. Robic, and V. Dzapo. 1999. Isolation and characterization of the porcine c-myc proto-oncogene and chromosomal assignment to SSC 4p13. Anim. Genet. 30:204-206. crossref(new window)

Schmidt, T. B., M. W. Schilling, J. M. Behrends, V. Battula, V. Jackson, R. K. Sekhon, and T. E. Lawrence. 2010. Use of cluster analysis and preference mapping to evaluate consumer acceptability of choice and select bovine M. longissimus lumborum steaks cooked to various end-point temperatures. Meat Sci. 84:46-53. crossref(new window)

Stalder, K. J., J. Maya, L. L. Christian, S. J. Moeller, and K. J. Prus. 1998. Effects of preslaughter management on the quality of carcasses from porcine stress syndrome heterozygous market hogs. J. Anim. Sci. 76:2435-2443.

Whitelaw, P. F. and J. E. Hesketh. 1992. Expression of c-myc and c-fos in rat skeletal muscle. Evidence for increased levels of c-myc mRNA during hypertrophy. Biochem. J. 281( Pt 1):143-147. crossref(new window)