Advanced SearchSearch Tips
Effect of Antioxidant Flavonoids (Quercetin and Taxifolin) on In vitro Maturation of Porcine Oocytes
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effect of Antioxidant Flavonoids (Quercetin and Taxifolin) on In vitro Maturation of Porcine Oocytes
Kang, Jung-Taek; Moon, Joon Ho; Choi, Ji-Yei; Park, Sol Ji; Kim, Su Jin; Saadeldin, Islam M.; Lee, Byeong Chun;
  PDF(new window)
Quercetin (QT) and taxifolin (TF) are structurally similar plant-derived flavonoids that have antioxidant properties and act as free radical scavengers. The objective of this study was to investigate effects of QT and TF on nuclear maturation of porcine oocytes. Effects of TF at 0, 1, 10, and on oocyte nuclear maturation (polar body extrusion) were investigated. After incubation for 44 h, there were no significant differences between the treatment and control groups except in the group which was significantly lower (59.2%, p<0.05) than the other groups (control: >80%). After parthenogenetic activation, further in vitro development of QT- or TF-treated vs control oocytes was investigated. A significantly higher proportion of QT-treated () oocytes developed into blastocysts compared to controls (24.3% vs 16.8%, respectively); however, cleavage rate and blastocyst cell number were not affected. The TF-treated group was not significantly different from controls. Levels of reactive oxygen species (ROS) and intracellular glutathione (GSH) in oocytes and embryos in a culture medium supplemented with QT or TF were measured. Both treatment groups had significantly lower (p<0.05) levels of ROS than controls, however GSH levels were different only in QT-treated oocytes. We conclude that exogenous flavonoids such as QT and TF reduce ROS levels in oocytes. Although at high concentration () both QT and TF appear to be toxic to oocytes.
In vitro Maturation;Porcine Oocyte;Antioxidants;Quercetin;Reactive Oxygen Species [ROS];
 Cited by
Progesterone influences cytoplasmic maturation in porcine oocytes developingin vitro, PeerJ, 2016, 4, e2454  crossref(new windwow)
Querectin Alleviates Zinc Oxide Nanoreprotoxicity in Male Albino Rats, Journal of Biochemical and Molecular Toxicology, 2016  crossref(new windwow)
Abeydeera, L. R., W. H. Wang, T. C. Cantley, R. S. Prather, and B. N. Day. 1998. Presence of beta-mercaptoethanol can increase the glutathione content of pig oocytes matured in vitro and the rate of blastocyst development after in vitro fertilization. Theriogenology 50:747-756. crossref(new window)

Bagchi, D., O. R. Carryl, M. X. Tran, M. Bagchi, A. Garg, M. M. Milnes, C. B. Williams, J. Balmoori, D. J. Bagchi, S. Mitra, and S. J. Stohs. 1999. Acute and chronic stress-induced oxidative gastrointestinal mucosal injury in rats and protection by bismuth subsalicylate. Mol. Cell Biochem. 196:109-116. crossref(new window)

Boquest, A. C., L. R. Abeydeera, W. H. Wang, and B. N. Day. 1999. Effect of adding reduced glutathione during insemination on the development of porcine embryos in vitro. Theriogenology 51:1311-1319. crossref(new window)

de Matos, D. G. and C. C. Furnus. 2000. The importance of having high glutathione (GSH) level after bovine in vitro maturation on embryo development: Effect of beta-mercaptoethanol, cysteine and cystine. Theriogenology 53:761-771. crossref(new window)

Galeati, G., C. Vallorani, D. Bucci, C. Bernardini, C. Tamanini, A. Parmeggiani, and M. Spinaci. 2010. Daidzein does affect progesterone secretion by pig cumulus cells but it does not impair oocytes IVM. Theriogenology 74:451-457. crossref(new window)

Guerin, P., S. El Mouatassim, and Y. Menezo. 2001. Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings. Hum. Reprod. Update 7:175-189.

Jefferson, W. N., E. Padilla-Banks, E. H. Goulding, S. P. Lao, R. R. Newbold, and C. J. Williams. 2009. Neonatal exposure to genistein disrupts ability of female mouse reproductive tract to support preimplantation embryo development and implantation. Biol. Reprod. 80:425-431.

Johnson, M. H. and M. H. Nasr-Esfahani. 1994. Radical solutions and cultural problems: could free oxygen radicals be responsible for the impaired development of preimplantation mammalian embryos in vitro? Bioessays 16:31-38. crossref(new window)

Kang, J. T., M. Atikuzzaman, D. K. Kwon, S. J. Park, S. J. Kim, J. H. Moon, O. J. Koo, G. Jang, and B. C. Lee. 2012. Developmental competence of porcine oocytes after in vitro maturation and in vitro culture under different oxygen concentrations. Zygote 20:1-8. crossref(new window)

Kang, J. T., O. J. Koo, D. K. Kwon, H. J. Park, G. Jang, S. K. Kang, and B. C. Lee. 2009. Effects of melatonin on in vitro maturation of porcine oocyte and expression of melatonin receptor RNA in cumulus and granulosa cells. J. Pineal Res. 46:22-28. crossref(new window)

Kang, J. T., D. K. Kwon, S. J. Park, S. J. Kim, J. H. Moon, O. J. Koo, G. Jang, and B. C. Lee. 2013. Quercetin improves the in vitro development of porcine oocytes by decreasing reactive oxygen species levels. J. Vet. Sci. 14:15-20. crossref(new window)

Kim, J. H., S. H. Lee, S. Kim, Y. W. Jeong, O. J. Koo, M. D. Hashem, S. M. Park, E. G. Lee, M. S. Hossein, S. K. Kang, B. C. Lee, and W. S. Hwang. 2006. Embryotrophic effects of ethylenediaminetetraacetic acid and hemoglobin on in vitro porcine embryos development. Theriogenology 66:449-455. crossref(new window)

Kure-bayashi, S., M. Miyake, K. Okada, and S. Kato. 2000. Successful implantation of in vitro-matured, electro-activated oocytes in the pig. Theriogenology 53:1105-1119. crossref(new window)

Kwon, J. H., S. B. Kim, K. H. Park, and M. W. Lee. 2011. Antioxidative and anti-inflammatory effects of phenolic compounds from the roots of Ulmus macrocarpa. Arch. Pharm. Res. 34:1459-1466. crossref(new window)

Makena, P. S., S. C. Pierce, K. T. Chung and S. E. Sinclair. 2009. Comparative mutagenic effects of structurally similar flavonoids quercetin and taxifolin on tester strains Salmonella typhimurium TA102 and Escherichia coli WP-2 uvrA. Environ. Mol. Mutagen. 50:451-459. crossref(new window)

Naderi, G. A., S. Asgary, N. Sarraf-Zadegan, and H. Shirvany. 2003. Anti-oxidant effect of flavonoids on the susceptibility of LDL oxidation. Mol. Cell Biochem. 246:193-196. crossref(new window)

Santini, S. E., G. Basini, S. Bussolati, and F. Grasselli. 2009. The phytoestrogen quercetin impairs steroidogenesis and angiogenesis in swine granulosa cells in vitro. J. Biomed. Biotechnol. Article ID 419891.

Tang, X., C. Zhang, W. Zeng, Y. Mi, and H. Liu. 2006. Proliferating effects of the flavonoids daidzein and quercetin on cultured chicken primordial germ cells through antioxidant action. Cell Biol. Int. 30:445-451. crossref(new window)

Tao, Y., B. Zhou, G. Xia, F. Wang, Z. Wu, and M. Fu. 2004. Exposure to L-ascorbic acid or alpha-tocopherol facilitates the development of porcine denuded oocytes from metaphase I to metaphase II and prevents cumulus cells from fragmentation. Reprod. Domest. Anim. 39:52-57. crossref(new window)

Telugu, B. P., T. Ezashi, S. Sinha, A. P. Alexenko, L. Spate, R. S. Prather, and R. M. Roberts. 2011. Leukemia inhibitory factor (LIF)-dependent, pluripotent stem cells established from inner cell mass of porcine embryos. J. Biol. Chem. 286:28948-28953. crossref(new window)

Whitaker, B. D. and J. W. Knight. 2004. Exogenous gammaglutamyl cycle compounds supplemented to in vitro maturation medium influence in vitro fertilization, culture, and viability parameters of porcine oocytes and embryos. Theriogenology 62:311-322. crossref(new window)

Yang, H. W., K. J. Hwang, H. C. Kwon, H. S. Kim, K. W. Choi, and K. S. Oh. 1998. Detection of reactive oxygen species (ROS) and apoptosis in human fragmented embryos. Hum. Reprod. 13:998-1002. crossref(new window)

You, J., J. Kim, J. Lim, and E. Lee. 2010. Anthocyanin stimulates in vitro development of cloned pig embryos by increasing the intracellular glutathione level and inhibiting reactive oxygen species. Theriogenology 74:777-785. crossref(new window)

Yuan, Z. P., L. J. Chen, L. Y. Fan, M. H. Tang, G. L. Yang, H. S. Yang, X. B. Du, G. Q. Wang, W. X. Yao, Q. M. Zhao, B. Ye, R. Wang, P. Diao, W. Zhang, H. B. Wu, X. Zhao, and Y. Q. Wei. 2006. Liposomal quercetin efficiently suppresses growth of solid tumors in murine models. Clin. Cancer Res. 12:3193-3199. crossref(new window)