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Effects of Steroid Hormone in Avian Follicles
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 Title & Authors
Effects of Steroid Hormone in Avian Follicles
Caicedo Rivas, R.E.; Nieto, M. Paz-Calderon; Kamiyoshi, M.;
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The aim of the present study was to examine the effects of testosterone (T) and estradiol- () on the production of progesterone () by granulosa cells, and of the on the production of and T by theca internal cells. In the first experiment, granulosa cells isolated from the largest () and third largest () preovulatory follicle were incubated for 4 h in short-term culture system, production by granulosa cells of both and was increased in a dose-dependent manner by ovine luteinizing hormone (oLH), but not T or . In the second experiment, and granulosa cells cultured for 48 h in the developed monolayer culture system were recultured for an additional 48 h with increasing doses of various physiological active substances existing in the ovary, including T and . Basal production for 48 h during 48 to 96 h of the cultured was about nine fold greater by granulosa cells than by granulosa cells. In substances examined oLH, chicken vasoactive intestinal polypeptide (cVIP) and T, but not , stimulated in a dose-dependent manner production in both and granulosa cells. In addition, when the time course of production by granulosa cells in response to oLH, cVIP, T and was examined for 48 h during 48 to 96 h of culture, although had no effect on production by granulosa cells of during the period from 48 to 96 h of culture, production with oLH was found to be increased at 4 h of the culture, with a maximal 9.14 fold level at 6 h. By contrast, production with cVIP and T increased significantly (p<0.05) from 8 and 12 h of the culture, respectively, with maximal 6.50 fold response at 12 h and 6, 48 fold responses at 36 h. Furthermore, when granulosa cells were precultured with for various times before 4 h culture with oLH at 96 h of culture, the increase in production in response to oLH with a dose-related manner was only found at a pretreatment time of more than 12 h. In the third experiment, theca internal cells of , and the largest third to fifth preovulatory follicles () were incubated for 4 h in short-term culture system with increasing doses of . The production of and T by theca internal cells were increased with the addition of of . These increases were greater in smaller follicles. These results indicate that, in granulosa cells of the hen, T may have a direct stimulatory action in the long term on production, and on in long-term action which may enhance the sensitivity to LH for production, and thus, in theca internal cells, in short term action may stimulate the production of and T.
Preovulatory Follicles;Steroids Hormone;Granulosa;Theca Internal and External Cells;
 Cited by
Armstrong, D. G. 1984. Ovarian aromatase activity in the domestic fowl (Gallus domesticus). J. Endocrinol. 100:81-86. crossref(new window)

Asem, E. K. and F. Hertelendy. 1986. Steroidogenesis and cAMP production in isolated avian granulosa cells during follicular maturation: Lack of positive correlation. Acta Endocrimol. 113:289-297.

Barano, J. L. and J. M. Hammond. 1985. Serum-free medium enhances growth and differentiation of cultured pig granulosa cells. Endocrinology 116:51-58. crossref(new window)

Barnes, J. L. S. and J. M. Hammond. 1980. Serum-free cell culture: A unifying approach. Cell 22:649-655. crossref(new window)

Caicedo, R. E. 2004. Steroidogenesis in theca cells of chicken follicles. Tecnociencia 6:71-83.

Caicedo, R. E., J. L. Zumaquero, and J. D. Quintero. 2005. Effect of estrogen in the progesterone production in granulosa cells. Scientia 17:47-56.

Calvo, F. O. and J. M. Bahr. 1983. Adenylyl cyclase system of the small preovulatory follicles of the domestic hen: Responsiveness to follicle-stimulating hormone and luteinizing hormone. Biol. Reprod. 29:542-547. crossref(new window)

Channing, C. P., V. Tsai, and D. Sachs. 1976. Role of insulin, cortisol in luteinization of porcine granulosa cells growth in chemically defined media. Biol. Reprod. 15:235-247. crossref(new window)

Chin, E. C. and D. R. E. Abayasecara. 2004. Progesterone secretion by luteinizing human granulosa cells: A possible cAMP-dependent but PKA-independent mechanism involved in its regulation. J. Endocrinol. 183:51-60. crossref(new window)

Erickson, G. F., C. Wang, and A. J. W. Hsuech. 1979. FSH induction of functional LH receptors in granulosa cells cultured in a chemically defined medium. Nature 279:336-338. crossref(new window)

Evans, G., M. Dobias, G. J. King, and D. T. Armstrong. 1981. Estrogen, androgen, and progesterone biosynthesis by theca and granulosa of preovulatory follicles in the pig. Biol. Reprod. 25:673-682. crossref(new window)

Freeman, D. A. 1985. Estradiol acts as a competitive inhibitor of the 3${\beta}$-hidroxysteroid dehydrogenase/${\Delta}^5-{\Delta}^4$ isomerase enzyme of cultured Leydig tumor cells. Endocrinology 117:2127-2133. crossref(new window)

Griffith, J. B. 1970. The effect of insulin on the growth and metabolism of the human diploid cell, WI-38. J. Cell Sci. 7:575-585.

Groothuis, T. G., W. Muller, N. von Engelhardt, C. Carere, and C. Eising. 2005. Maternal hormones as a tool to adjust offspring phenotype in avian species. Neurosci. Biobehav. Rev. 29:329-352. crossref(new window)

Ham, R. G. 1965. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc. Natl. Acad. Sci. 53:288-293. crossref(new window)

Hammond, R. W., D. W. Olson, R. B. Frenkel, H. V. Biellier, and F. Hertelendy. 1980. Prostaglandins and steroid hormones in plasma and ovarian follicles during the ovulation cycle of the domestic hen (Gallus domesticus). Gen. Comp. Endocrinol. 42:195-202. crossref(new window)

Hertelendy, F., B. Nemecz, and E. K. Asem, 1987. Influence of follicular maturation on LH-promoted $Ca^{2+}$ mobilization phosphoinositide hydrolysis in granulosa cells of the domestic fowl. Biol. Reprod. Suppl. 1:125.

Heuson, J. C., A. Coune, and R. Heimann. 1967. Cell proliferation induced by insulin in organ culture of rat mammary carcinoma. Exp. Cell Res. 45:351-360. crossref(new window)

Hsueh, A. J. W., P. B. C. Jones, E. Y. Adashi, C. Wang, L. Z. Zhuang, and T. H. J. Welsh. 1983. Intraovarian mechanisms in the hormonal control of granulosa cell differentiation in rats. J. Reprod. Fertil. 69:325-342. crossref(new window)

Huang, E. S. R. and A. V. Nalbandov. 1979. Steroidogenesis of chicken granulosa theca cells: in vitro incubation system. Biol. Reprod. 20:442-453. crossref(new window)

Johnson, A. L. 1990. Steroidogenesis and actions of steroids in the hen ovary. Cri. Rev. Poult. Biol. 2:319-346.

Johnson, A. L. and J. T. Bridghan. 2001. Regulation of steroidogenic acute regulatory protein and luteinizing hormone receptor messenger ribonucleic acid in hen granulosa cells. Endocrinology 142:3116-3124. crossref(new window)

Johnson, A. L., Z. Li, J. A. Gibney, and S. Malamed. 1994. Vasoactive intestinal peptide-induced expression of cytochrome P-450 cholesterol side-chain cleavage $17{\alpha}$-hydroxylase enzyme activity in hen granulosa cells. Biol. Reprod. 51:327-333. crossref(new window)

Johnson, A. L. and J. L. Tilly. 1988. Effects of vasoactive intestinal peptide on steroid secretion and plasminogen activator activity in granulosa cells of the hen. Biol. Reprod. 38:296-303. crossref(new window)

Johnson, P. A., C. Green, H. T. Lee, and J. M. Bahr. 1988. Inhibition of progesterone secretion from granulosa cells by estradiol and androgens in the domestic hen. Endocrinology 123:473-477. crossref(new window)

Kamiyoshi, M., M. Kawashima, and K. Tanaka. 1992. Estrogens promote cultured granulosa cells of the hen for progesterone production in response to LH. Jpn. Poult. Sci, 29:91-97. crossref(new window)

Kamiyoshi, M., T. Niwa, and K. Tanaka. 1986. Nuclear estrogen receptor bindings in granulosa cells estradiol-$17{\beta}$ in follicular membranes of the ovary of the hen during the ovulatory cycle. Gen. Comp. Endocrinol. 61:428-435. crossref(new window)

Kikushi, M. and S. Ishii. 1992. Changes in luteinizing hormone receptors in the granulosa and theca layers of the ovarian follicle during follicular maturation in the Japanese quail. Gen. Comp. Endocrinol. 85:124-137. crossref(new window)

Knecht, M., C. H. T. Morris, and K. J. Catt. 1985. Estrogen dependence of luteinizing hormone receptor expression in cultured rat granulosa cells. Inhibition of granulosa cell development by the antiestrogens tamoxifen and keoxifene. Endocrinology 116:1771-1777. crossref(new window)

Krzysik-Walker, S. M., Olga M. Ocon-Grove, S. B. Maddineni, G. L. Hendricks III, and R. Ramachandran. 2007. Identification of calcitonin expression in the chicken ovary: Influence of follicular maturation and ovarian steroids. Biol. Reprod. 4:626-635.

Lee, H. T. and J. M. Bahr. 1989. Inhibitory sites of androgens and estradiol in progesterone biosynthesis in granulosa cells of the domestic hen. Endocrinology 125:760-765. crossref(new window)

Li, X., H. Peegel, and K. M. J. Menon. 2001. Regulation of high density lipoprotein receptor messenger ribonucleic acid expression and cholesterol transport in theca-interstitial cells by insulin and human chorionic gonadotropin. Endocrinology 142:174-181. crossref(new window)

Marrone, B. L. and F. Hertelendy. 1983. Steroidogenesis by avian ovarian cells: Effects of luteinizing hormone and substrate availability. Am. J. Physiol. 244:E487-E493.

Mikami, S. 1980. Hypothalamic control of the avian adenohypophysis. In: Biological Rhythmus in Birds: Neural Endocrine Aspects (Eds. Y. Tanabe, K Tanaka, and T. Ookawa). Jp. Sc. Soc. Press, Springer-Verlag, Heidelberg, Berlin, Germany. 17-32.

Orly, J., G. Sato, and G. F. Erickson. 1980. Serum suppresses the expression of hormonally induced functions in cultured granulosa cells. Cell 20:817-827. crossref(new window)

Perry, M. M., A. B. Gilbert, and A. J. Evans. 1978. Electron microscope observations on the ovarian follicle of the domestic fowl during the rapid growth phase. J. Anat. 125:481-497.

Porter, T. E., B. M. Hargis, J. L. Silsby, and M. E. El Halawani. 1989. Differential steroid production between theca interna and theca externa cells: A three-cell model for follicular steroidogenesis in avian species. Endocrinology 125:109-116. crossref(new window)

Richards, J. S., J. J. Ireland, M. C. Rao, G. A. Bernath, A. R. J. Midgley, and L. E. J. Reichert. 1976. Ovarian follicular development in the rat: Hormone receptor regulation by estradiol, follicle stimulating hormone and luteinizing hormone. Endocrinology 99:1562-1570. crossref(new window)

Ritzhaup, L. K. and J. M. Bahr. 1987. A decrease in FSH receptors of granulosa cells during follicular maturation in the domestic hen. J. Endocrinol. 115:303-310. crossref(new window)

Robinson, F. E. and R. A. Renema. 1999. Principios del manejo de los foto periodos en Reproductoras de engorde. Editorial Cobb-Vantress Incorporation, Arkansas, USA. Boletin Tecnico 7:1-6.

Rothwell, B. and S. E. Salomon. 1977. The ultrastructure of the follicle wall of the domestic fowl during the phase of rapid growth. Br. Poult. Sci. 18:605-610. crossref(new window)

Schally, A. V., R. M. Nair, T. W. Redding, and A. Arimura. 1971. Isolation of the luteinizing hormone and follicle-stimulating hormone-releasing hormone from porcine hypothalamus. J. Biol. Chem. 23:7230-7236.

Seol, H. S., K Sato, Y. Matsubara, W. J. Schneider, and Y. Akiba. 2007. Modulation of sterol regulatory element binding protein-2 in response to rapid follicle development in chickens. Comp. Biochem. Physiol. Part B: Biochem. Mol. Biol. 147:698-703. crossref(new window)

Von Engelhard, N. K. and T. G. G. Groothuis. 2005. Measuring steroid hormones in avian eggs. Ann. NY Acad. Sci. 1046: 181-192. crossref(new window)

Wang, S. C. and J. M. Bahr. 1983. Estradiol secretion by theca cells of the domestic hen during the ovulatory cycle. Biol. Reprod. 28:618-624. crossref(new window)

Wells, J. W., H. R. Dick, and A. B. Gilbert. 1981. The biosynthesis of progesterone by fowl granulosa cells in vitro from $^{14}C$-labelled substrates. J. Steroid Biochem. 14:651-656. crossref(new window)

Williams, J. B. and P. J. Sharp. 1978. Control of the preovulatory surge of luteinizing hormone in the hen (Gallus domesticus): The role of progesterone and androgens. J. Endocrinol. 77:57-65. crossref(new window)

Woods, D. C. and A. L. Johnson. 2005. Regulation of folliclestimulating hormone-receptor messenger RNA in hen granulosa cells relative to follicle selection. Biol. Reprod. 72:643-650. crossref(new window)

Woods, D. C., M. J. Haugen, and A. L. Johnson. 2007. Actions of epidermal growth factor receptor/mitogen-activated protein kinase and protein kinase C signaling in granulose cells from Gallus gallus are dependent upon stage of differentiation. Biol. Reprod. 77:61-70. crossref(new window)

Wu, Q., S. Sucheta, A. Azhar, and K. M. J. Menon. 2003. Lipoprotein enhancement of ovarian theca-interstitial cell steroidogenesis: Relative contribution of scavenger receptor class B (type I) and adenosine 5- triphosphate-binding cassette (type A1) transporter in high-density lipoprotein-cholesterol transport and androgen synthesis. Endocrinology 144:2437-2445. crossref(new window)

Yoshimura, Y., T. Okamoto, and T. Tamura. 1995. Effects of luteinizing hormone follicle-stimulating hormone on the progesterone receptor induction in chicken granulosa cells in vivo. Poult. Sci. 74:147-151. crossref(new window)

Zarrow, M. X. and J. W. Bastian. 1953. Blockade of ovulation in the hen with adrenolytic and parasympatholytic drugs. Proc. Soc. Exp. Biol. Med. 84:457-459. crossref(new window)