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Effects of Steroid Hormone in Avian Follicles
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 Title & Authors
Effects of Steroid Hormone in Avian Follicles
Caicedo Rivas, R.E.; Nieto, M. Paz-Calderon; Kamiyoshi, M.;
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The aim of the present study was to examine the effects of testosterone (T) and estradiol- () on the production of progesterone () by granulosa cells, and of the on the production of and T by theca internal cells. In the first experiment, granulosa cells isolated from the largest () and third largest () preovulatory follicle were incubated for 4 h in short-term culture system, production by granulosa cells of both and was increased in a dose-dependent manner by ovine luteinizing hormone (oLH), but not T or . In the second experiment, and granulosa cells cultured for 48 h in the developed monolayer culture system were recultured for an additional 48 h with increasing doses of various physiological active substances existing in the ovary, including T and . Basal production for 48 h during 48 to 96 h of the cultured was about nine fold greater by granulosa cells than by granulosa cells. In substances examined oLH, chicken vasoactive intestinal polypeptide (cVIP) and T, but not , stimulated in a dose-dependent manner production in both and granulosa cells. In addition, when the time course of production by granulosa cells in response to oLH, cVIP, T and was examined for 48 h during 48 to 96 h of culture, although had no effect on production by granulosa cells of during the period from 48 to 96 h of culture, production with oLH was found to be increased at 4 h of the culture, with a maximal 9.14 fold level at 6 h. By contrast, production with cVIP and T increased significantly (p<0.05) from 8 and 12 h of the culture, respectively, with maximal 6.50 fold response at 12 h and 6, 48 fold responses at 36 h. Furthermore, when granulosa cells were precultured with for various times before 4 h culture with oLH at 96 h of culture, the increase in production in response to oLH with a dose-related manner was only found at a pretreatment time of more than 12 h. In the third experiment, theca internal cells of , and the largest third to fifth preovulatory follicles () were incubated for 4 h in short-term culture system with increasing doses of . The production of and T by theca internal cells were increased with the addition of of . These increases were greater in smaller follicles. These results indicate that, in granulosa cells of the hen, T may have a direct stimulatory action in the long term on production, and on in long-term action which may enhance the sensitivity to LH for production, and thus, in theca internal cells, in short term action may stimulate the production of and T.
Preovulatory Follicles;Steroids Hormone;Granulosa;Theca Internal and External Cells;
 Cited by
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